CN212819967U - Liquid separating device for culture medium - Google Patents

Liquid separating device for culture medium Download PDF

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Publication number
CN212819967U
CN212819967U CN202021018644.0U CN202021018644U CN212819967U CN 212819967 U CN212819967 U CN 212819967U CN 202021018644 U CN202021018644 U CN 202021018644U CN 212819967 U CN212819967 U CN 212819967U
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China
Prior art keywords
bottle
culture medium
port
interface
way valve
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Expired - Fee Related
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CN202021018644.0U
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Chinese (zh)
Inventor
张坤灵
谢峻
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Maanshan Teachers College
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Maanshan Teachers College
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Priority to CN202021018644.0U priority Critical patent/CN212819967U/en
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Abstract

The utility model discloses a liquid device is divided to culture medium. This divide liquid device can include: the three-way valve comprises a first interface, a second interface and a third interface, the sampling bottle is connected with the first interface through a first connecting pipe, the liquid storage bottle comprises a first port and a second port, the first port is connected with the second interface of the three-way valve, and the culture bottle is connected with the third interface of the three-way valve through a second connecting pipe. The utility model discloses a three-way valve can make sample bottle and stock solution bottle intercommunication, perhaps make stock solution bottle and blake bottle intercommunication, when sample bottle and stock solution bottle intercommunication, can follow the second port of stock solution bottle through outside drawing liquid equipment and take a sample the inside culture medium suction of bottle to the stock solution bottle, when stock solution bottle and blake bottle intercommunication, carry the culture medium in the stock solution bottle to the blake bottle in, thereby the transfer of culture medium has been accomplished, and the culture medium is at the in-process that shifts, it is short to expose at external time, the risk that receives the pollution has been reduced.

Description

Liquid separating device for culture medium
Technical Field
The utility model belongs to the culture medium field of handling, more specifically relates to a culture medium divides liquid device.
Background
In the biochemical teaching of school and the production of industrial nursery, the liquid separation operation of culture medium is often required to be carried out in a clean bench. In the liquid separation process, the staff need not only ensure that the liquid separation of the culture medium can be quantitative and accurate, but also reduce the risk of pollution caused by exposure of the culture medium in the environment outside the system as much as possible in the process of subpackaging the sterilized liquid culture medium into a plurality of culture bottles. However, the existing operating equipment has the following problems: the pipetting quantity of the culture medium is inaccurate, and the pipetting quantity is inconvenient and intuitive to read; in the pipetting process, the culture medium in the blue-cap bottle is residual, which causes waste; in the liquid transferring process, the bottle mouth is exposed to the environment outside the system for a long time in the liquid separating process, so that the pollution risk is increased; the existing automation device has higher cost and can not be flexibly adapted to the simple liquid distribution device.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a liquid device is divided to culture medium realizes reducing the probability that the liquid medium received the pollution in the experiment to reduce equipment cost.
In order to achieve the above object, the utility model provides a culture medium liquid separating device, which comprises,
a three-way valve;
the sampling bottle is connected with a first interface of the three-way valve through a first connecting pipe;
the liquid storage bottle comprises a first port and a second port, and the first port is connected with the second interface of the three-way valve;
and the culture bottle is connected with the third interface of the three-way valve through a second connecting pipe.
Optionally, a tampon is included, the tampon being disposed within the second port.
Optionally, the liquid absorbing device is further included, and the extraction end of the liquid absorbing device is connected with the second port.
Optionally, the imbibition device is electronic pipette, electronic pipette's suction head with the second port is connected.
Optionally, the imbibition device is the aurilave, the suction nozzle of aurilave with the second port is connected.
Optionally, the bottom of the inner cavity of the sampling bottle is provided with a drainage block, and the drainage block is provided with a conical groove.
Optionally, one end of the first connection pipe is connected to the first interface, and the other end of the first connection pipe extends into the sampling bottle and is located at the bottom end of the tapered groove.
Optionally, the first connecting pipe is made of glass.
Optionally, the liquid storage bottle is a transparent liquid storage bottle, and a dial gauge is arranged on the liquid storage bottle.
Optionally, one end of the second connecting pipe is connected to the third interface, and the other end of the second connecting pipe extends into the culture bottle and is located at the upper part of the inner cavity of the culture bottle.
The beneficial effects of the utility model reside in that, can make sample bottle and stock solution bottle intercommunication through the three-way valve, perhaps make stock solution bottle and blake bottle intercommunication, when sample bottle and stock solution bottle intercommunication, can follow the second port of stock solution bottle through outside drawing liquid equipment and will take a sample the inside culture medium suction of bottle to the stock solution bottle, when stock solution bottle and blake bottle intercommunication, carry the culture medium in the stock solution bottle to the blake bottle in, thereby the transfer of culture medium has been accomplished, and the culture medium is at the in-process that shifts, it is short to expose at external time, the risk that receives the pollution has been reduced.
Other features and advantages of the present invention will be described in detail in the detailed description which follows.
Drawings
The above and other objects, features and advantages of the present invention will become more apparent by describing in more detail exemplary embodiments thereof with reference to the attached drawings, in which like reference numerals generally represent like parts throughout the exemplary embodiments of the present invention.
Fig. 1 shows a schematic structural diagram of a culture medium separating device according to an embodiment of the present invention.
Fig. 2 shows a schematic structural diagram of a liquid storage bottle and a scale of a culture medium liquid separation device according to an embodiment of the present invention.
In the figure: 1-sampling bottle, 2-liquid storage bottle, 3-culture bottle, 4-three-way valve, 5-first connecting pipe, 6-second connecting pipe, 7-absorbent cotton plug, 8-electric liquid-transferring gun, 9-ear washing ball, 10-drainage block and 11-scale meter.
Detailed Description
Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
A culture medium liquid separating device comprises a liquid separating device,
a three-way valve;
the sampling bottle is connected with a first interface of the three-way valve through a first connecting pipe;
the liquid storage bottle comprises a first port and a second port, and the first port is connected with the second interface of the three-way valve.
The culture bottle is connected with the third interface of the three-way valve through a second connecting pipe.
Can make sample bottle and stock solution bottle intercommunication through the three-way valve, perhaps make stock solution bottle and blake bottle intercommunication, when sample bottle and stock solution bottle intercommunication, can follow the second port of stock solution bottle through outside drawing liquid equipment and take a sample the inside culture medium suction of bottle to the stock solution bottle, when stock solution bottle and blake bottle intercommunication, carry the culture medium in the stock solution bottle to the blake bottle in, thereby the transfer of culture medium has been accomplished, and the culture medium is at the in-process that shifts, it is short to expose at external time, the risk that receives the pollution has been reduced.
Fig. 1 shows a structural schematic diagram of a culture medium divides liquid device according to an embodiment of the utility model, and fig. 2 shows a structural schematic diagram of a stock solution bottle and a scale of a culture medium divides liquid device according to an embodiment of the utility model.
Examples
A culture medium liquid separating device comprises a liquid separating device,
a three-way valve 4;
the sampling bottle 1 is connected with a first interface of the three-way valve 3 through a first connecting pipe 5, and the volume specification of the sampling bottle 1 can be 50ml, 100ml, 200ml, 500ml or 1000 ml;
the liquid storage bottle 2, the liquid storage bottle 2 includes first port and second port, the second interface connection of first port and three-way valve 4.
The culture bottle 3, culture bottle 3 passes through second connecting pipe 6 and the third interface connection of three-way valve 4.
The three-way valve 4 in this embodiment is a common three-way valve 4 on the market, and the three-way valve 4 is provided with a three-way valve 4 knob, and a user can control the conduction state of the three-way valve 4 by rotating the three-way valve 4 knob.
Specifically, the absorbent tampon 7 is further included, the absorbent tampon 7 is arranged in the second port, and the second port can be made to be breathable but not completely closed by inserting the absorbent tampon 7 into the second port, so that the liquid suction device can be conveniently extended into the second port to draw liquid.
Particularly, still include the suction means, the extraction end and the second port of suction means are connected, and the suction means is used for absorbing the culture medium in the sampling bottle 1 to the stock solution bottle 2 in.
Particularly, the imbibition device is electronic pipette 8, and electronic pipette 8's suction head is connected with the second port, and electronic pipette 8 power is big, convenient to use, and the imbibition is fast, can be with the quick suction of the culture medium in the sampling bottle 1 to the stock solution bottle 2 in.
Particularly, the suction device is aurilave 9, and aurilave 9's suction nozzle is connected with the second port, and aurilave 9 is manual suction device, can inhale the culture medium in the sample bottle 1 to stock solution bottle 2 through user's manually operation aurilave 9, and aurilave 9 cost is cheap, and is with low costs.
Specifically, the bottom of the inner cavity of the sampling bottle 1 is provided with a drainage block 10, the drainage block 10 is provided with a conical groove, and the tip of the conical groove is positioned at the bottom of the sampling bottle 1. The liquid suction device can conveniently draw the liquid in the sampling bottle 1 through the drainage block 10.
Particularly, the one end and the first interface connection of first connecting pipe 5, the other end of first connecting pipe 5 stretch into in sampling bottle 1 and be located the bottom department of bell jar, and first connecting pipe 5 stretches into the bottom that reaches the bell jar and can make the imbibition effect better, reduces the liquid residue in the sampling bottle 1.
Specifically, the first connecting pipe 5 is made of glass.
Specifically, the liquid storage bottle 2 is a transparent liquid storage bottle 2, the liquid storage bottle 2 is provided with a scale 11, the amount of liquid extracted from the liquid storage bottle 2 can be better observed through the scale 11, so that the liquid separation work of the culture medium is more accurate, and the liquid storage bottle 2 is also provided with adaptive specification and size according to the specification and actual requirements of the sampling bottle 1; since the volume of the liquid storage bottle 2 released into the culture bottle 3 is calculated by adopting a liquid releasing method for reading the liquid separation volume, the scale 11 of the liquid storage bottle 2 adopts inverted scales, namely, the scale numerical value is gradually increased from 0 from top to bottom, so that the liquid separation volume can be visually read only by keeping the liquid storage bottle 2 vertical without other posture adjustment in the liquid separation process.
Specifically, the one end and the third interface connection of second connecting pipe 6, the other end of second connecting pipe 6 stretch into 3 interior and lie in 3 inner chamber upper portions departments of blake bottle, and the second connecting pipe 6 that stretches into blake bottle 3 should not be too short, should not too long in inserting 3 bottles of blake bottle. When the time is too short, the bottle cap is easy to slide out, so that the exposure risk of the environment outside the system is caused; when the length is too long, the liquid surface of the liquid culture medium released into the culture bottle 3 is not extended into the pipe orifice of the second connecting pipe 6 of the culture bottle 3, which easily causes suck-back and causes pollution.
The utility model discloses a use method as follows:
before the device uses, connect the installation with whole set of device, sampling bottle 1 is connected with three-way valve 4, is connected by second connecting pipe 6 between three-way valve 4 and blake bottle 3, and the equal opening of lower extreme on the stock solution bottle 2, and three-way valve 4 is connected to the below opening part, and the second port is stopped up with medical absorbent tampon 7, makes it both ventilative, but unlikely to totally enclosed, cooperates aurilave 9 (or electronic pipetting gun 8) again to realize inhaling, putting the liquid.
The above-described complete set of devices (except the ear washing bulb 9 or the electric pipette 8) was sterilized in a high-pressure steam cooker and then transferred to a clean bench.
The first connecting tube 5 is inserted into the bottom of the sampling bottle 1 to ensure that all the culture medium in the sampling bottle 1 can be transferred and separated at the end of the separation.
The knob of the three-way valve 4 is rotated to communicate the liquid storage bottle 2 with the sampling bottle 1, the ear washing ball 9 (or the electric pipette 8) is used for sucking, and the liquid culture medium in the sampling bottle 1 is sucked to the scale liquid storage bottle 2 through the first connecting pipe 5 by using pressure difference.
The knob of the three-way valve 4 is rotated to communicate the liquid storage bottle 2 with the culture bottle 3, liquid is discharged by natural gravity (or discharged by the electric liquid-transferring gun 8) according to the dial gauge 11 on the night storage bottle, the liquid discharging amount is read visually, and the liquid is discharged according to actual needs.
The culture bottle 3 is replaced, the liquid culture medium in the scale liquid storage bottle 2 is continuously released, and continuous liquid release for multiple times can be realized.
While various embodiments of the present invention have been described above, the above description is intended to be illustrative, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.

Claims (10)

1. A culture medium liquid separating device is characterized by comprising,
a three-way valve;
the sampling bottle is connected with a first interface of the three-way valve through a first connecting pipe;
the liquid storage bottle comprises a first port and a second port, and the first port is connected with the second interface of the three-way valve;
and the culture bottle is connected with the third interface of the three-way valve through a second connecting pipe.
2. A device for distributing media as recited in claim 1 further comprising a tampon pledget disposed within said second port.
3. The device for dispensing media of claim 1 further comprising a pipetting device, wherein the withdrawal end of the pipetting device is connected to the second port.
4. The device for dispensing media of claim 3 wherein the pipetting device is an electric pipette having a pipette tip connected to the second port.
5. The device for separating culture medium according to claim 3, wherein the liquid absorbing device is an aurilave, and the suction nozzle of the aurilave is connected with the second port.
6. The culture medium separating device according to claim 1, wherein a drainage block is arranged at the bottom of the inner cavity of the sampling bottle, and a conical groove is arranged on the drainage block.
7. The device for separating culture medium according to claim 6, wherein one end of the first connecting tube is connected to the first interface, and the other end of the first connecting tube extends into the sampling bottle and is located at the bottom end of the conical groove.
8. The device for separating culture medium according to claim 7, wherein the first connecting tube is made of glass.
9. The culture medium separating device according to claim 1, wherein the liquid storage bottle is a transparent liquid storage bottle, and a scale is arranged on the liquid storage bottle.
10. The culture medium separating device according to claim 1, wherein one end of the second connecting pipe is connected to the third interface, and the other end of the second connecting pipe extends into the culture bottle and is located at the upper part of the inner cavity of the culture bottle.
CN202021018644.0U 2020-06-05 2020-06-05 Liquid separating device for culture medium Expired - Fee Related CN212819967U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021018644.0U CN212819967U (en) 2020-06-05 2020-06-05 Liquid separating device for culture medium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021018644.0U CN212819967U (en) 2020-06-05 2020-06-05 Liquid separating device for culture medium

Publications (1)

Publication Number Publication Date
CN212819967U true CN212819967U (en) 2021-03-30

Family

ID=75170730

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202021018644.0U Expired - Fee Related CN212819967U (en) 2020-06-05 2020-06-05 Liquid separating device for culture medium

Country Status (1)

Country Link
CN (1) CN212819967U (en)

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Granted publication date: 20210330