CN212459099U

CN212459099U - Portable chromosome staining device

Info

Publication number: CN212459099U
Application number: CN202021279335.9U
Authority: CN
Inventors: 雷良玉; 杨正亮
Original assignee: Luohe First Peoples Hospital
Current assignee: Luohe First Peoples Hospital
Priority date: 2020-07-04
Filing date: 2020-07-04
Publication date: 2021-02-02
Anticipated expiration: 2030-07-04

Abstract

The utility model provides a portable chromosome dyeing apparatus has effectively solved chromosome dyeing apparatus's among the prior art not enough, the problem of a large amount of dyeings simultaneously, has solved the problem that mechanized glass cover and avoid producing the bubble simultaneously, and the technical scheme of its solution is, can make the plummer submergence that bears glass slide guarantee to dye fully in dyeing solution suitable time including intermittent drive device, and the agitating unit who is equipped with can make dyeing solution misce bene make the dyeing even, and the cover glass device that is equipped with can mechanize and realize covering glass cover to the effectual bubble that avoids producing of cover glass covers from one side slope, the utility model discloses not only the structure is ingenious, and is simple and practical simultaneously again.

Description

Portable chromosome staining device

Technical Field

The utility model relates to a chromosome dyeing apparatus, especially portable chromosome dyeing apparatus.

Background

Chromosome staining is a work often done in experiments, but is usually performed manually at present, and the staining is performed manually, so that the staining efficiency is low, and a situation of short supply and short demand can occur when a large amount of chromosome staining is needed.

Chromosome staining methods are various, and staining can be performed by using an appropriate method for adherent cells, suspension cells, tissue sections and the like, wherein when staining suspension cells, the general steps are as follows:

centrifuging to collect cell sample and centrifuging tube;

adding a proper amount of fixing solution to slowly suspend the cells for a proper time;

centrifuging to remove the fixing solution, and washing twice with PBS or sodium chloride solution with concentration of nine percent;

after the last centrifugation, sucking most of the liquid and keeping a proper amount of liquid, slowly suspending the cells, and dripping the cells onto the glass slide to ensure that the cells are uniformly distributed as much as possible;

slightly drying the cell to ensure that the cell is stuck on the glass slide and is not easy to flow along with liquid;

staining with 0.5ml Hoechst 33258 staining solution;

a clean cover glass is covered to avoid bubbles as much as possible.

When staining the suspension cells, the cell suspension should be dropped onto the slide from an appropriate height to ensure successful staining.

SUMMERY OF THE UTILITY MODEL

In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a portable chromosome dyeing device, which effectively solves the defects of the chromosome dyeing device in the prior art, and can not simultaneously dye a large amount of chromosomes, and simultaneously solves the problems of mechanization covering a cover glass and avoiding bubbles generation, and the technical proposal of the solution is that the portable chromosome dyeing device comprises a box body, and is characterized by comprising an intermittent transmission device fixedly connected at the left end of the box body, wherein an output shaft of the intermittent transmission device is transversely arranged and extends into the box body, a plurality of bevel gears which are uniformly arranged at intervals are fixedly connected on the output shaft, a bearing shaft which is longitudinally and rotatably connected in the box body is uniformly in gear transmission on each bevel gear, a bearing platform is fixedly connected on each bearing platform, a slide fixing device is fixedly connected on the upper end surface of each bearing platform, and a dyeing tank which is filled with dyeing solution is arranged below the bearing, lie in the box trailing flank and go up the rotation and be connected with a plurality of cover plate devices, each cover plate device is corresponding with each plummer position, and horizontal arrangement is gone up through the reversal motor drive's of fixed connection on the box major axis of horizontal arrangement on the box trailing flank, fixed connection between major axis and each cover plate device lower extreme, and the level is arranged between major axis position and each plummer up end, and equal fixed connection sucking disc is gone up to each cover plate device, and the sucking disc is all controlled through adsorption equipment.

Preferably, the intermittent transmission device comprises a driving motor fixedly connected to the left end of the box body, a short shaft which is connected with the box body in a rotating mode and driven by the driving motor and transversely arranged, a guide disc is fixedly connected to the right end of the short shaft, a sector groove is coaxially formed in the guide disc, a poking rod which is fixedly connected to the left end of the guide disc and coincides with an angular bisector of the sector groove, a short column which is transversely arranged is fixedly connected to the lower end of the poking rod, a special-shaped triangular block fixedly connected with an output shaft, an outer edge face of the special-shaped triangular block is an arc face capable of being in sliding fit with an outer edge face of the guide disc, and three sliding grooves capable of being in.

Preferably, slide glass fixing device includes the draw-in groove that is used for placing the slide glass that the plummer upper end was seted up, and the spacing groove has been seted up to the draw-in groove left end, has seted up the transverse arrangement groove of sliding respectively on the both sides wall around the spacing groove, and sliding fit has longitudinal arrangement's grip block in the groove of sliding, and grip block upper end left side is equipped with dials the board, and grip block rear end and spacing groove left side wall pass through spring coupling, and the plummer right side is equipped with the baffle that left end and draw-in groove right side wall parallel and level just are located the grip block right.

Preferably, the cover slip device comprises a placing plate fixedly connected with the long shaft at the lower end, a placing groove used for placing the cover slip is formed in the front end of the placing plate, the depth of the placing groove is larger than the thickness of the cover slip so as to facilitate the matching of the glass slide and the cover slip, sponge layers are fixedly connected to the left side wall and the right side wall of the placing groove respectively, through grooves matched with the glass slide fixing device are formed in the left side wall and the right side wall of the placing groove respectively, and the cover slip device is controlled to rotate along the long shaft through an adsorption device.

Preferably, the adsorption device comprises a supporting plate fixedly connected with the rear end of the box body and transversely arranged, and an air pressure cylinder fixedly connected with the supporting plate and controlled by an electric controller, wherein the air pressure cylinder is connected with the sucker through an air guide hose.

Preferably, still including being used for carrying out the agitating unit that stirs to the dyeing tank, agitating unit is including being used for carrying out the agitating unit that stirs to the dyeing tank, agitating unit including be located dyeing tank the place ahead and with each a plurality of gear wheels that bear axle fixed connection, the equal gear drive of each gear wheel has the pinion, has a plurality of axis of rotation of longitudinal rotation connection in the box through each pinion transmission, the equal fixedly connected with stirring board of a plurality of evenly arranging in each axis of rotation.

Preferably, the device is characterized by further comprising a trigger device for controlling the cover plate device to move, wherein the trigger device comprises a trigger rod fixedly connected to the bearing shaft and located between the front of the dyeing bath and the large gear, an arc contact piece fixedly connected to the front end of the dyeing bath and located above the left side of the bearing shaft and matched with the trigger rod, an electric controller is started when the trigger rod is matched with the arc contact piece and controls the reverse motor and the adsorption device to be started, the reverse motor and the adsorption device stop running when the trigger rod is separated from the arc contact piece, and the cover plate device resets after the cover plate device stays for a set time.

Preferably, a through groove for limiting the height of the dyeing solution is formed in the upper side of the right end of the dyeing tank, and a faucet for discharging the dyeing solution is fixedly connected to the lower side of the dyeing tank.

Preferably, the device further comprises a dropping rack for dropping liquid to the glass slide, and the upper end of the dropping rack is provided with a plurality of limiting holes which can correspond to the center of the glass slide one by one.

The utility model has the advantages that:

1. the drop staining can be simultaneously carried out on a plurality of glass slides, and the experiment with larger demand can be met;

2. the arranged intermittent device ensures that the glass slide can be dyed for a long time, so that the dyeing is uniform;

3. the stirring device can uniformly mix the dyeing solution and ensure uniform dyeing;

4. the cover glass device that is equipped with has realized automatic cover glass, has alleviateed the manpower, and the production of bubble has been avoided to the cover glass from one side lid simultaneously.

Drawings

Fig. 1 is a perspective view of the first view angle of the present invention.

Fig. 2 is a perspective view of the utility model with the second visual angle of the baffle at the upper end and the right end of the box body removed.

Fig. 3 is an enlarged schematic view of the area a in the second perspective view of the present invention.

Fig. 4 is the schematic diagram of the clamping block and the sliding groove of the present invention.

Fig. 5 is a schematic view of the utility model in full section from the right.

Fig. 6 is an enlarged schematic view of the B region in the full-section right-view schematic view of the present invention.

Fig. 7 is a schematic view of the utility model of a full-section left side.

Fig. 8 is an enlarged schematic view of the C region in the full-section left-view schematic view of the present invention.

Fig. 9 is the utility model discloses special-shaped triangle piece and deflector complex sketch map.

Reference numerals: 1. the automatic dyeing machine comprises a box body, 2. an output shaft, 3. a bevel gear, 4. a bearing shaft, 5. a bearing table, 6. a dyeing groove, 7. a reverse motor, 8. a long shaft, 9. a sucking disc, 10. a driving motor, 11. a short shaft, 12. a guide disc, 13. a sector groove, 14. a poking rod, 15. a short column, 16. a special-shaped triangular block, 17. an arc surface, 18. a sliding groove, 19. a clamping groove, 20. a limiting groove, 21. a sliding groove, 22. a clamping block, 23. a poking plate, 24. a spring, 25. a baffle, 26. a placing plate, 27. a placing groove, 28. a sponge layer, 29. a through groove, 30. a supporting plate, 31. a pneumatic cylinder, 32. an air guide hose, 33. a large gear, 34. a small gear, 35. a rotating shaft, 36. a stirring plate, 37. a trigger rod, 38. an arc-shaped contact piece, 39. a leakage groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention. The embodiment of the utility model provides an in the attached drawing: the different types of hatching in the figures are not given the national standards, do not require the material of the elements, and distinguish between cross-sectional views of the elements in the figures.

The following description of the present invention will be made in detail with reference to the accompanying drawings 1 to 9.

The first embodiment adopts the technical scheme that the device comprises a box body 1 and is characterized by comprising an intermittent transmission device fixedly connected to the left end of the box body 1, an output shaft 2 of the intermittent transmission device is transversely arranged and extends into the box body 1, a plurality of bevel gears 3 uniformly arranged at intervals are fixedly connected to the output shaft 2, bearing shafts 4 longitudinally and rotatably connected into the box body 1 are in gear transmission on each bevel gear 3, bearing platforms 5 are fixedly connected to each bearing shaft 4, a slide glass fixing device is fixedly connected to the upper end face of each bearing platform 5, a dyeing tank 6 filled with dyeing solution is arranged below each bearing platform 5 in the box body 1, a plurality of cover plate devices are rotatably connected to the rear side face of the box body 1, each cover plate device corresponds to each bearing platform 5 in position, a long shaft 8 which is transversely arranged on the rear side face of the box body 1 and is driven by a reverse motor 7 fixedly connected onto the box body 1, fixed connection between major axis 8 and each cover plate device lower extreme, level between 8 axis positions of major axis and each plummer 5 up end arrange, equal fixed connection sucking disc 9 on each cover plate device, sucking disc 9 all controls through adsorption equipment. When the embodiment is used, a glass slide is placed in the clamping groove 19 on the bearing platform 5 and fixed by the fixing device, a cell suspension is dripped to the center position of the glass slide through the limiting hole 42, the cell suspension is dried slightly to enable the cell to be attached to the glass slide and not to flow along with liquid easily, the intermittent transmission device is started, the intermittent transmission device drives the output shaft 2 to rotate anticlockwise by one hundred twenty degrees and then stop rotating for a set time, the bevel gear 3 fixedly connected to the output shaft 2 transmits motion to the bearing shaft 4 longitudinally and rotatably connected in the box body 1 through gear transmission along with the anticlockwise rotation of the output shaft 2, the bearing shaft 4 rotates clockwise by one hundred twenty degrees and then stops rotating for a set time, at the moment, the bearing platform 5 fixedly connected with the bearing shaft 4 also rotates clockwise by one hundred twenty degrees, and the glass slide fixed at the upper end of the bearing platform 5 is immersed in a staining solution in the staining tank 6 and keeps the, the output shaft 2 rotates counterclockwise one hundred twenty degrees again after the output shaft stops rotating for a set time and stops rotating for a second time, the bevel gear 3 fixedly connected with the output shaft 2 transmits the motion to the bearing shaft 4 longitudinally and rotationally connected in the box body 1 through gear transmission, so that the bearing shaft 4 rotates clockwise one hundred twenty degrees for a second time and stops rotating for a set time, the bearing platform 5 fixedly connected with the bearing shaft 4 rotates clockwise one hundred twenty degrees for a second time, the glass slide fixed at the upper end of the bearing platform 5 is immersed in the dyeing solution in the dyeing tank 6 and keeps the set time, the output shaft 2 stops rotating after rotating counterclockwise one hundred twenty degrees again after the stop set time along with the continuous operation of the intermittent device, the bevel gear 3 fixedly connected with the output shaft 2 transmits the motion to the bearing shaft 4 longitudinally and rotationally connected in the box body 1 through gear transmission, the bearing shaft 4 is rotated clockwise one hundred twenty degrees for the third time and stops rotating, the bearing platform 5 fixedly connected with the bearing shaft 4 rotates clockwise one hundred twenty degrees for the third time and is fixed on the upper end surface of the bearing platform 5 and faces upwards again and is the same as the initial state, the trigger device is started in the third rotation process of the bearing shaft 4 to enable the electric controller to be switched on and control the intermittent device to stop rotating and start the cover glass device and the adsorption device, the cover glass device adsorbs the cover glass by the sucker 9, the cover glass device rotates anticlockwise around a long shaft 8 fixedly connected with the cover glass device and is matched with the bearing platform 5 to enable the cover glass to cover the glass slide, the adsorption device stops operating under the control of the electric controller at the moment and does not adsorb the cover glass, and the cover glass device resets under the action of a reversing motor 7 controlled by the electric controller.

In the second embodiment, on the basis of the first embodiment, the intermittent transmission device includes a driving motor 10 fixedly connected to the left end of the box 1, a short shaft 11 rotationally connected to the box 1 and driven by the driving motor 10 and transversely arranged, a guide disc 12 is fixedly connected to the right end of the short shaft 11, a sector groove 13 is coaxially formed in the guide disc 12, a toggle rod 14 fixedly connected to the left end of the guide disc 12 and coinciding with an angular bisector of the sector groove 13, a short column 15 transversely arranged is fixedly connected to the lower end of the toggle rod 14, a special-shaped triangular block 16 fixedly connected to the output shaft 2, an outer edge surface of the special-shaped triangular block 16 is an arc surface 17 capable of being in sliding fit with an outer edge surface of the guide disc 12, and three sliding grooves 18 capable of being in sliding fit with the short column 15 are uniformly formed in the special. When the special-shaped triangular block is used, one arc surface 17 of the special-shaped triangular block 16 is matched with the outer edge surface of the guide disc 12, the driving motor 10 is started, the driving motor 10 transmits power to the guide disc 12 through the short shaft 11 which is rotationally connected with the box body 1 and transversely arranged, so that the guide disc 12 rotates in an opposite direction and a clockwise direction, the poke rod 14 which is fixedly connected with the left end of the guide disc 12 and is superposed with the angular bisector of the fan-shaped groove 13 starts to rotate clockwise, the short column 15 which is fixedly connected with the lower end of the poke rod 14 and transversely arranged at the lower end of the poke rod 14 and one sliding groove 18 on the special-shaped triangular block 16 start to be matched, poked to rotate the special-shaped triangular block 16 and transmit power to the output shaft 2 along with the clockwise rotation of the poke rod 14, the arc surface 17 of the special-shaped triangular block 16 is gradually separated from the outer edge surface of the guide disc 12, the special-shaped triangular block 16 does not rotate any more and power is not transmitted to the output shaft 2 any more, meanwhile, the other circular arc surface 17 of the special-shaped triangular block 16 is matched with the outer edge surface of the guide disc 12 to keep the special-shaped triangular block 16 in a stop state, when the guide disc 12 rotates clockwise for one circle, the transversely arranged short column 15 fixedly connected with the lower end of the poke rod 14 is matched with the other sliding groove 18 on the special-shaped triangular block 16 to poke the special-shaped triangular block 16 to rotate again and transmit power to the output shaft 2, simultaneously the circular arc surface 17 of the special-shaped triangular block 16 is gradually separated from matching with the outer edge surface of the guide disc 12 along with the rotation of the special-shaped triangular block 16, when the guide disc 12 rotates clockwise for one hundred twenty degrees again, the short column 15 is separated from matching with the sliding groove 18, the special-shaped triangular block 16 does not rotate any more and power is not transmitted to the output shaft 2, simultaneously, the third circular arc surface 17 of the special-shaped, when the guide disc 12 rotates clockwise for two circles, the short column 15 which is fixedly connected with the lower end of the poke rod 14 and transversely arranged and is fixedly connected with the third sliding groove 18 on the special-shaped triangular block 16 start to be matched, the special-shaped triangular block 16 is poked to rotate for the third time, power is transmitted to the output shaft 2, meanwhile, the arc surface 17 of the special-shaped triangular block 16 gradually disengages from the outer edge surface of the guide disc 12 along with the rotation of the special-shaped triangular block 16, when the guide disc 12 rotates clockwise for one hundred twenty degrees again, the short column 15 disengages from the sliding groove 18, the special-shaped triangular block 16 does not rotate any more, the power is not transmitted to the output shaft 2 any more, if the driving motor 10 does not stop rotating, the process is repeated and the guide disc 12 rotates for.

Third embodiment, on the basis of first embodiment, slide glass fixing device includes that the draw-in groove 19 that is used for placing the slide glass that 5 upper ends of plummer seted up, spacing groove 20 has been seted up to draw-in groove 19 left end, transverse arrangement sliding groove 21 has been seted up on the both sides wall around spacing groove 20 respectively, sliding fit has longitudinal arrangement's grip block 22 in sliding groove 21, grip block 22 right-hand member face stretches out slightly to the draw-in groove 19 in, grip block 22 upper end left side is equipped with dials movable plate 23, grip block 22 rear end is connected through spring 24 with spacing groove 20 left side wall, 5 right sides of plummer are equipped with the baffle 25 of left end and draw-in groove 19 right side wall parallel and level. When the glass slide fixing device is used, the poking plate 23 is pushed rightwards by a hand, the poking plate 23 drives the clamping block 22 fixedly connected with the poking plate to move leftwards along the sliding groove 21 and compress the spring 24, a glass slide is placed in the clamping groove 19, the upper end face of the glass slide is slightly higher than the clamping groove 19 so as to be convenient for taking out the glass slide after dyeing is completed, the poking plate 23 is loosened, the clamping block 22 moves rightwards along the sliding groove 21 and is matched with the left end of the glass slide under the action of the spring 24, the glass slide is located in the clamping groove 19 at the moment, and the left end and the right end of the glass slide are respectively matched with the right end of the clamping block 22 and the.

Fourth embodiment, on the basis of first embodiment, the cover slip device includes that lower extreme and major axis 8 fixed connection's place board 26, place board 26 front end and offer the standing groove 27 that is used for placing the coverslip, standing groove 27 degree of depth is greater than coverslip thickness so that slide and coverslip cooperation, the left and right sides wall of standing groove 27 respectively fixedly connected with sponge layer 28, the left and right sides wall of standing groove 27 is seted up respectively can with slide fixing device complex logical groove 29, the cover slip device carries out its rotation motion along major axis 8 through adsorption equipment control. When the embodiment is used, the adsorption device is started, the cover glass is placed in the placing groove 27, the lower end of the cover glass is matched with the lower end of the placing groove 27, the rear end of the cover glass is adsorbed and fixed in the placing groove 27 by the sucking disc 9 controlled by the adsorption device, after the glass slide is dyed and the trigger device is triggered, the trigger device is controlled by the electric controller to start and drive the long shaft 8 to drive the cover glass device to rotate downwards to be matched with the bearing table 5, the through grooves 29 on the left side and the right side of the cover glass device are respectively matched with the fixing device and the baffle 25, the upper end of the glass slide is matched with the front end of the cover glass, the sponge layers 28 fixedly connected with the left side wall and the right side wall of the placing groove 27 are respectively matched with the left end and the right end of the matched cover glass and the left end and the right end of the matched glass slide to attract dyeing solution to enable dyeing to be more sufficient, after the time that the cover slip device is matched with the bearing table 5, the adsorption device is closed under the control of the electric controller which is switched on by the trigger device, and meanwhile, the reverse motor 7 reverses to reset the cover slip device.

Fifth embodiment, on the basis of the first embodiment, the suction device includes a horizontally disposed supporting plate 30 fixedly connected to the rear end of the box 1, an air cylinder 31 fixedly connected to the supporting plate 30 and controlled by an electric controller, and the air cylinder 31 is connected to the suction cup 9 through an air hose 32. When the cover glass placing device is used, the adsorption device is started to control the air pressure cylinder 31 to suck air, so that the suction disc 9 connected with the air pressure cylinder 31 through the air guide hose 32 generates suction force and adsorbs a cover glass into the placing groove 27, after the cover glass device is matched with the bearing table 5 for a set time, the adsorption device is closed under the control of the electric controller connected with the trigger device, and the suction disc 9 loses the adsorption effect on the cover glass.

Sixth embodiment, on the basis of first embodiment, still include the agitating unit that is used for stirring dyeing tank 6, agitating unit includes a plurality of gear wheels 33 that are located dyeing tank 6 the place ahead and with each bear axle 4 fixed connection, and each gear wheel 33 all gear drive has pinion 34, has a plurality of axis of rotation 35 of longitudinal rotation connection in box 1 through each pinion 34 transmission, all fixedly connected with a plurality of stirring boards 36 of evenly arranging on each axis of rotation 35. This embodiment is when using, output shaft 2 is in the rotation state with power transmission for bearing axle 4 through gear transmission and makes bearing axle 4 clockwise rotation, make simultaneously with bearing axle 4 fixed connection's gear wheel 33 clockwise rotation, gear wheel 33 makes its rotation with power transmission for axis of rotation 35 through the pinion 34 rather than the meshing, dyeing solution mixes fully under the stirring board 36 effect with axis of rotation 35 fixed connection to gear wheel 33 and pinion 34 cooperate can let axis of rotation 35 rotate the more stirring effect of number of times better through the drive ratio.

Seventh embodiment, on the basis of fourth embodiment, still include the trigger device that is used for controlling the cover plate device to carry out the motion, trigger device includes fixed connection and is located the dyeing tank 6 the place ahead and the gear wheel 33 on bearing shaft 4 trigger lever 37, fixed connection is in the dyeing tank 6 front end and be located bearing shaft 4 upper left side can cooperate with trigger lever 37 arc contact 38, when trigger lever 37 cooperates with arc contact 38 electrical controller start and control the reverse installation motor and drive the cover plate device to rotate and cooperate with plummer 5 and accomplish the cover plate, when trigger lever 37 and arc contact 38 break away from the cooperation the electrical controller control cover plate device stops to close the adsorption equipment after setting for the time, reverse the reversal motor 7 reversal makes the cover plate device reset simultaneously. When the embodiment is used, when power is transmitted to the bearing shaft 4 through the output shaft 2 by gear transmission to enable the bearing shaft 4 to rotate clockwise to approach one circle, the upper end of the trigger rod 37 fixedly connected to the bearing shaft 4 and positioned between the front of the dyeing tank 6 and the large gear 33 starts to be in sliding fit with the arc contact piece 38 and starts the electric controller, the electric controller controls the reverse motor to drive the cover piece device to rotate to be matched with the bearing platform 5 to complete the cover piece, when the trigger rod 37 is separated from the arc contact piece 38, the electric controller controls the cover piece device to stop for a set time and then closes the adsorption device, and meanwhile, the reverse motor 7 reverses to reset the cover piece device.

In the eighth embodiment, in addition to the first embodiment, a drain tank 39 for limiting the height of the dyeing solution is provided on the upper side of the right end of the dyeing tank 6, and a tap 40 for discharging the dyeing solution is fixedly connected to the lower side of the dyeing tank 6. In use, the embodiment is provided with a tank as a volume reference for the staining solution, the staining solution should be close to the drain tank 39 to ensure that the upper end surface of the bearing table 5 is not submerged and the staining solution can submerge the slide glass when the slide glass stays after being turned over, and the tap 40 can be opened to discharge the staining solution when the staining solution needs to be discharged.

Ninth embodiment, on the basis of the first embodiment, the slide glass further comprises a drip rack 41 for dripping the slide glass, and the upper end of the drip rack 41 is provided with a plurality of limiting holes 42 which can correspond to the centers of the slide glass one by one. When the embodiment is used, the height of the dropping liquid rack 41 is suitable for dropping the cell suspension, and the limiting hole 42 can be used for assisting the cell suspension to drop at the center of the glass slide.

Claims

1. A portable chromosome dyeing device comprises a box body (1), and is characterized by comprising an intermittent transmission device fixedly connected at the left end of the box body (1), an output shaft (2) of the intermittent transmission device is transversely arranged and extends into the box body (1), a plurality of bevel gears (3) which are uniformly arranged at intervals are fixedly connected onto the output shaft (2), a bearing shaft (4) which is longitudinally and rotatably connected into the box body (1) is arranged on each bevel gear (3), a bearing platform (5) is fixedly connected onto each bearing shaft (4), a slide glass fixing device is fixedly connected onto the upper end surface of each bearing platform (5), a dyeing tank (6) filled with dyeing solution is arranged below the bearing platform (5) in the box body (1), a plurality of cover plate devices are rotatably connected onto the rear side surface of the box body (1), and each cover plate device corresponds to each bearing platform (5), the long shaft (8) is transversely arranged on the rear side face of the box body (1) and driven by a reverse motor (7) fixedly connected to the box body (1), the long shaft (8) is fixedly connected with the lower ends of the cover plate devices, the axial positions of the long shaft (8) and the upper end faces of the bearing tables (5) are horizontally arranged, sucking discs (9) are fixedly connected to the cover plate devices, and the sucking discs (9) are controlled through an adsorption device.

2. The portable chromosome staining apparatus of claim 1, wherein the intermittent drive means comprises a drive motor (10) fixedly attached to a left end of the case (1), a short shaft (11) which is rotationally connected with the box body (1) and is driven by a driving motor (10) and transversely arranged, a guide disc (12) is fixedly connected at the right end of the short shaft (11), a fan-shaped groove (13) is coaxially arranged on the guide disc (12), a poke rod (14) which is fixedly connected at the left end of the guide disc (12) and is superposed with the angular bisector of the fan-shaped groove (13), a short column (15) which is transversely arranged is fixedly connected at the lower end of the poke rod (14), the special-shaped triangular block (16) is fixedly connected with the output shaft (2), the outer edge surface of the special-shaped triangular block (16) is an arc surface (17) which can be in sliding fit with the outer edge surface of the guide disc (12), and three sliding grooves (18) which can be in sliding fit with the short columns (15) are uniformly formed in the special-shaped triangular block (16).

3. The portable chromosome staining device according to claim 1, wherein the slide glass fixing device comprises a clamping groove (19) which is formed in the upper end of the bearing table (5) and used for placing a slide glass, a limiting groove (20) is formed in the left end of the clamping groove (19), sliding grooves (21) which are transversely arranged are formed in the front side wall and the rear side wall of the limiting groove (20) respectively, a clamping block (22) which is longitudinally arranged is arranged in the sliding grooves (21) in a sliding fit mode, the right end face of the clamping block (22) slightly extends into the clamping groove (19), a poking plate (23) is arranged on the left side of the upper end of the clamping block (22), the rear end of the clamping block (22) is connected with the left side wall of the limiting groove (20) through a spring (24), and a baffle (25) which is flush with the right side wall of.

4. The portable chromosome staining device according to claim 1, wherein the cover slip device comprises a placing plate (26) with a lower end fixedly connected with the long shaft (8), a placing groove (27) for placing a cover slip is formed in the front end of the placing plate (26), the depth of the placing groove (27) is larger than the thickness of the cover slip so as to facilitate the matching of the slide and the cover slip, sponge layers (28) are fixedly connected to the left side wall and the right side wall of the placing groove (27), through grooves (29) capable of being matched with the slide fixing device are formed in the left side wall and the right side wall of the placing groove (27), and the cover slip device is controlled to rotate along the long shaft (8) through an adsorption device.

5. The portable chromosome staining device according to claim 1, wherein the adsorption device comprises a horizontally arranged supporting plate (30) fixedly connected with the rear end of the box body (1), an air pressure cylinder (31) fixedly connected with the supporting plate (30) and controlled by an electric controller, and the air pressure cylinder (31) is connected with the suction cup (9) through an air guide hose (32).

6. The portable chromosome staining device according to claim 1, further comprising a stirring device for stirring the staining tank (6), wherein the stirring device comprises a plurality of large gears (33) which are positioned in front of the staining tank (6) and fixedly connected with the respective bearing shafts (4), each large gear (33) is in gear transmission with a small gear (34), a plurality of rotating shafts (35) which are longitudinally and rotatably connected in the box body (1) are in gear transmission with the respective small gear (34), and a plurality of uniformly arranged stirring plates (36) are fixedly connected to each rotating shaft (35).

7. The portable chromosome dyeing device according to claim 4, characterized by further comprising a trigger device for controlling the movement of the cover plate device, wherein the trigger device comprises a trigger rod (37) fixedly connected to the bearing shaft (4) and positioned between the front of the dyeing bath (6) and the large gear (33), an arc contact piece (38) which is fixedly connected to the front end of the dyeing bath (6) and positioned above the left side of the bearing shaft (4) and can be matched with the trigger rod (37), when the trigger rod (37) is matched with the arc contact piece (38), the electric controller starts and controls the reverse motor and the adsorption device to start, when the trigger rod (37) is disengaged from the arc contact piece (38), the reverse motor and the adsorption device stop running, and the cover plate device resets after the set time.

8. The portable chromosome staining device according to claim 1, wherein a leakage groove (39) for limiting the height of the staining solution is formed on the upper side of the right end of the staining tank (6), and a water tap (40) for discharging the staining solution is fixedly connected to the lower side of the staining tank (6).

9. The portable chromosome staining device according to claim 1, further comprising a drip rack (41) for dripping the slide glass, wherein the upper end of the drip rack (41) is provided with a plurality of limiting holes (42) which can correspond to the center of the slide glass one by one.