CN212293544U - Bio-generator for nitrobacteria culture - Google Patents
Bio-generator for nitrobacteria culture Download PDFInfo
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- CN212293544U CN212293544U CN202020497864.XU CN202020497864U CN212293544U CN 212293544 U CN212293544 U CN 212293544U CN 202020497864 U CN202020497864 U CN 202020497864U CN 212293544 U CN212293544 U CN 212293544U
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Abstract
The utility model discloses a biological generator is used in nitrobacteria cultivation, including the fermentation cylinder, be used for carrying out the feed supplement device of feed supplement and be used for carrying out the suction filtration device of suction filtration to the culture solution in the fermentation cylinder to the fermentation cylinder, the suction filtration device includes hollow fiber membrane module, drawing liquid control assembly, back flush subassembly and outer washing assembly. The utility model discloses a biological generator is used in nitrobacteria cultivation, be provided with feed supplement device and suction filtration device, can change the culture solution in the fermentation cylinder, make in the fermentation cylinder nitrite nitrogen and nitrate nitrogen concentration keep in lower concentration, make the bacterial can last high-efficient cultivation, suction filtration device is provided with back flush subassembly and outer washing assembly moreover, can carry out the back flush and wash the outside of hollow fiber membrane to the inside of hollow fiber membrane subassembly, it is smooth and easy to keep the hollow fiber membrane subassembly to switch on, the suction filtration of being convenient for goes on smoothly.
Description
Technical Field
The utility model relates to an environmental protection microbial technology field, concretely relates to biological generator is used in nitrobacteria cultivation.
Background
Nitrifying bacteria include nitrosobacteria and nitrifying bacteria. The nitrifying bacteria can convert ammonia nitrogen in the water body into nitrite nitrogen, and then the nitrifying bacteria can convert the nitrite nitrogen into nitrate nitrogen. The generation time of the nitrifying bacteria is shorter than that of the nitrifying bacteria in the growth process, which indicates that the growth rate of the nitrifying bacteria is faster, so that the generation rate of nitrite nitrogen in the culture solution is faster than the further oxidation rate of nitrifying bacteria in the culture process of the composite nitrifying bacteria. The nitrite nitrogen is accumulated in the nutrient solution, meanwhile, the nitrifying bacteria grow to generate the accumulation of nitrate nitrogen, and the nitrite nitrogen and the nitrate nitrogen in the culture solution can inhibit the activities of the nitrifying bacteria and the nitrifying bacteria when the concentrations of the nitrite nitrogen and the nitrate nitrogen reach certain degrees. When the concentration of nitrite nitrogen and nitrate nitrogen is excessively accumulated, the high-efficiency growth of the composite nitrifying bacteria liquid is obviously not facilitated.
CN101709278A uses activated sludge and nitrifying bacteria enrichment nutrient solution to enrich nitrosobacteria, and utilizes the flocculating settling to replace the nutrient solution in the culture process to discharge high-concentration nitrite nitrogen and nitrate nutrient solution, and replaces new nutrient solution to improve the thallus concentration. However, adding the flocculating agent, and the processes of precipitation and water change need time, which affects the culture efficiency of the nitrifying bacteria.
CN106434412A discloses the use of membrane filtration to separate culture fluids so that they can be cultured in a connected manner for mass production.
However, the traditional membrane filtration is easy to block in the separation and filtration, often needs to be disassembled for cleaning, is easy to interrupt the culture time of strains, and cannot achieve continuous culture.
SUMMERY OF THE UTILITY MODEL
The utility model aims at the above-mentioned among the prior art not enough, a biomass generator for nitrobacteria cultivation is provided, be provided with feed supplement device and suction filtration device, can change the culture solution in the fermentation tank, make in the fermentation tank nitrite nitrogen and nitrate nitrogen concentration keep in lower concentration, make the bacterial can last high-efficient cultivation, and the suction filtration device is provided with back flush subassembly and outer washing assembly, can carry out the back flush to the inside of hollow fiber membrane subassembly and wash the outside of hollow fiber membrane, it is smooth and easy to keep the hollow fiber membrane subassembly to switch on, the suction filtration of being convenient for goes on smoothly.
The purpose of the utility model is realized through the following technical scheme:
a biological generator for nitrobacteria culture comprises a fermentation tank, a material supplementing device used for supplementing materials to the fermentation tank and a suction filtration device used for suction filtration of culture solution in the fermentation tank, wherein the suction filtration device comprises a hollow fiber membrane component, a liquid pumping control component connected with the hollow fiber membrane component, a backwashing component used for backwashing the interior of the hollow fiber membrane component and an external washing component used for washing the outer side of the hollow fiber membrane component.
The liquid pumping control assembly comprises a liquid outlet diaphragm pump connected with the hollow fiber membrane assembly and a first electromagnetic valve used for controlling the opening and closing between the liquid outlet diaphragm pump and the hollow fiber membrane assembly.
The back washing component comprises an air pump connected with the hollow fiber membrane component and a second electromagnetic valve used for controlling the opening and closing of the air pump and the hollow fiber membrane component.
The outer washing component comprises an ultrafiltration membrane aeration pipe and a third electromagnetic valve, the ultrafiltration membrane aeration pipe is used for washing the outer side of the hollow fiber membrane component, the third electromagnetic valve is connected with the ultrafiltration membrane aeration pipe, the other end of the third electromagnetic valve is connected with an air pump, one end of the ultrafiltration membrane aeration pipe is communicated with the third electromagnetic valve, the other end of the ultrafiltration membrane aeration pipe extends to the bottom of the hollow fiber membrane component, and a small hole is formed in the side wall of the end.
Wherein, the feed supplement device includes feed supplement bucket, feed supplement pipe and is used for carrying the feed liquid diaphragm pump to the fermentation cylinder with feed liquid in the feed supplement bucket, the one end of feed supplement pipe extends the bottom of feed supplement bucket, and the other end of feed supplement pipe extends the fermentation tank bottom.
The biomass generator is characterized in that the biomass generator is further provided with an aeration device, the aeration device comprises a fourth electromagnetic valve, an air inlet pipe and a bottom aeration assembly, one end of the air inlet pipe is connected with an air pump, the other end of the air inlet pipe extends into the fermentation tank and is connected with the bottom aeration assembly, the fourth electromagnetic valve controls the opening and closing of the air inlet pipe, and one end, far away from the air inlet pipe, of the bottom aeration assembly is detachably connected with the feed supplementing pipe.
The bottom aeration component comprises a first aeration branch pipe, a second aeration branch pipe, a third aeration branch pipe, a fourth aeration branch pipe and a cross four-way joint, one end of the first aeration branch pipe is communicated with the air inlet pipe, the other end of the first aeration branch pipe is communicated with the cross four-way joint, one ends of the second aeration branch pipe, the third aeration branch pipe and the fourth aeration branch pipe are respectively communicated with the cross four-way joint, the second aeration branch pipe, the third aeration branch pipe and the fourth aeration branch pipe are arranged in a sealing mode, and one end, far away from the cross four-way joint, of the second aeration branch pipe is detachably connected with the material supplementing pipe.
Wherein, the first aeration branch pipe, the second aeration branch pipe, the third aeration branch pipe and the fourth aeration branch pipe are provided with aeration holes towards the bottom of the fermentation tank.
Wherein, biomass generator is provided with the electrode subassembly that is used for responding to the interior liquid level of fermentation cylinder, electrode subassembly includes probe, liquid level electrode upper probe and public electrode probe under the liquid level electrode, and probe, liquid level electrode upper probe and public electrode probe all are connected with the feed liquor diaphragm pump electricity under the liquid level electrode.
The utility model has the advantages that: the utility model discloses a biological generator is used in nitrobacteria cultivation, be provided with feed supplement device and suction filtration device, can change the culture solution in the fermentation cylinder, make in the fermentation cylinder nitrite nitrogen and nitrate nitrogen concentration keep in lower concentration, make the bacterial can last high-efficient cultivation, suction filtration device is provided with back flush subassembly and outer washing assembly moreover, can carry out the back flush and wash the outside of hollow fiber membrane to the inside of hollow fiber membrane subassembly, it is smooth and easy to keep the hollow fiber membrane subassembly to switch on, the suction filtration of being convenient for goes on smoothly.
Drawings
The invention is further described with the aid of the accompanying drawings, in which, however, the embodiments do not constitute any limitation to the invention, and for a person skilled in the art, other drawings can be derived from the following drawings without inventive effort.
Fig. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic structural diagram of the feeding device of the present invention.
Fig. 3 is a schematic structural diagram of the suction filtration device of the present invention.
Fig. 4 is a schematic structural view of the aeration device of the present invention.
Fig. 5 is a schematic view of the bottom aeration assembly of the present invention.
The reference numerals include: 1. a fermentation tank; 2. a material supplementing device; 21. a material supplementing barrel; 22. a material supplementing pipe; 23. a liquid inlet diaphragm pump; 3. a suction filtration device; 31. a hollow fiber membrane module; 32. a fluid withdrawal control assembly; 321. a first solenoid valve; 322. a liquid outlet diaphragm pump; 33. a backwash assembly; 331. a second solenoid valve; 332. an air pump; 34. an outer flushing assembly; 341. an ultrafiltration membrane aeration pipe; 342. a third electromagnetic valve; 4. an aeration device; 41. a fourth solenoid valve; 42. an air inlet pipe; 43. a bottom aeration assembly; 431. a first aeration branch pipe; 432. a second aeration branch pipe; 433. a third aeration branch pipe; 434. a fourth aeration branch pipe; 435. a cross four-way joint; 5. a liquid level electrode lower probe; 6. a probe on the liquid level electrode; 7. a common electrode probe.
Detailed Description
The present invention will be further described with reference to the following examples and accompanying drawings.
As shown in fig. 1 and 3, the utility model discloses a biological generator is used in nitrobacteria cultivation, including fermentation cylinder 1, be used for carrying out the feed supplement device 2 of feed supplement and be used for carrying out the suction filtration device 3 of suction filtration to the culture solution in the fermentation cylinder 1 to fermentation cylinder 1, suction filtration device 3 is including the hollow fiber membrane module 31 that is used for the suction filtration, the liquid control assembly 32 of drawing that is connected with hollow fiber membrane module 31, be used for carrying out the back flush subassembly 33 of back flush and be used for carrying out the outer washing subassembly 34 that washes to the hollow fiber membrane module 31 outside to hollow fiber membrane module 31 inside. Traditional biomass generator only is provided with hollow fiber membrane module 31, and hollow fiber membrane module 31 filters the culture solution in the fermentation cylinder 1, has used for a long time easy the jam, needs frequent washing maintenance or change, leads to the bacterium can not high-efficient continuous cultivation. The utility model discloses a biomass generator has the back flush subassembly 33 that is used for carrying out the back flush to hollow fiber membrane module 31 inside and is used for carrying out the outer washing subassembly 34 that washes in the hollow fiber membrane module 31 outside to add, can wash hollow fiber membrane module 31, avoids hollow fiber membrane module 31 to block up, makes biomass generator can last the high efficiency and carry out the bacterial cultivation.
As shown in fig. 1 and 3, the liquid suction control module 32 of the present invention includes a liquid outlet diaphragm pump 322 connected to the hollow fiber membrane module 31, and a first electromagnetic valve 321 for controlling the opening and closing between the liquid outlet diaphragm pump 322 and the hollow fiber membrane module 31. During operation, go out liquid diaphragm pump 322 and start, go out liquid diaphragm pump 322 and come out the culture solution suction filtration in the fermentation cylinder 1, when through hollow fiber membrane module 31, hollow fiber membrane module 31 filters and keeps the isolation bacterial and keep the bacterial and continue the fermentation in fermentation cylinder 1, and high concentration's nitrite nitrogen and nitrate nitrogen flow are unblocked and flow fermentation cylinder 1, reach the effect of suction filtration. The first electromagnetic valve 321 is arranged between the liquid outlet diaphragm pump 322 and the hollow fiber membrane module 31, and the first electromagnetic valve 321 controls the on-off between the liquid outlet diaphragm pump 322 and the hollow fiber membrane module 31 to control the liquid extraction.
As shown in fig. 1 and 3, the backwash module 33 of the present invention includes an air pump 332 connected to the hollow fiber membrane module 31, and a second electromagnetic valve 331 for controlling opening and closing between the air pump 332 and the hollow fiber membrane module 31. The air pump 332 is started, the air pump 332 introduces air into the hollow fiber membrane module 31, so that the air enters the hollow fiber membrane module 31 from the outside and overflows from the hollow fiber membrane module 31, the air performs air backwashing on the hollow fiber membrane module 31, the smoothness of membrane holes of the hollow fiber membrane module 31 is kept, and the blockage of the hollow fiber membrane module 31 is avoided. The second electromagnetic valve 331 controls the on/off of the air pump 332 and the hollow fiber membrane module 31 to control the progress and stop of backwashing. Preferably, the air pump 332 is provided with a plurality of output ports, enabling multipurpose use.
As shown in fig. 1 and 3, the external washing module 34 of the present invention includes an ultrafiltration membrane aeration pipe 341 for washing the outside of the hollow fiber membrane module 31, and a third electromagnetic valve 342 connected to the ultrafiltration membrane aeration pipe 341, and the other end of the third electromagnetic valve 342 is connected to the air pump 332. The air pump 332 is connected with the ultrafiltration membrane aeration pipe 341, the air pump 332 is started and inputs air to the ultrafiltration membrane aeration pipe 341, the air enters the fermentation tank 1 through the ultrafiltration membrane aeration pipe 341 and overflows from the culture solution to form bubbles, and the bubbles rise upwards and wash the outer side of the hollow fiber membrane module 31, so that the membrane pores of the hollow fiber membrane module 31 are smooth, and the blockage of the hollow fiber membrane module 31 is avoided. Specifically, one end of the filter membrane aeration pipe is communicated with the third electromagnetic valve 342, the other end of the ultrafiltration membrane aeration pipe 341 extends to the bottom of the hollow fiber membrane module 31, and a small hole is formed in the side wall of the end.
As shown in FIG. 1 and FIG. 2, the feeding device 2 of the present invention comprises a feeding barrel 21, a feeding tube 22 and a liquid inlet diaphragm pump 23 for conveying the feed liquid in the feeding barrel 21 to the fermentation tank 1, wherein one end of the feeding tube 22 extends to the bottom of the feeding barrel 21, and the other end of the feeding tube 22 extends to the bottom of the fermentation tank 1. After the suction filtration of the suction filtration device 3, the culture solution in the fermentation tank 1 is reduced, the feeding device 2 is started to supplement the culture solution in the fermentation tank 1, so that the culture solution in the fermentation tank 1 is kept at a certain amount and the concentrations of nitrate nitrogen and nitrite nitrogen are maintained at lower concentrations, and the continuous and efficient culture of the strains is ensured.
As shown in FIG. 1 and FIG. 4, the biomass generator of the present invention is further provided with an aeration device 4, the aeration device 4 comprises a fourth electromagnetic valve 41, an air inlet pipe 42 and a bottom aeration component 43, one end of the air inlet pipe 42 is connected with an air pump 332, the other end of the air inlet pipe 42 extends into the fermentation tank 1 and is connected with the bottom aeration component 43, the fourth electromagnetic valve 41 is used for controlling the opening and closing of the air inlet pipe 42, and one end of the bottom aeration component 43, which is far away from the air inlet pipe 42, is detachably connected with the feeding pipe 22. The aeration device 4 can aerate the fermentation tank 1 to provide air for the strains, specifically, the air pump 332 is started, the air pump 332 inputs air to the bottom aeration component 43 through the air inlet pipe 42, the air overflows from the bottom aeration component 43 to form bubbles, so as to aerate the culture solution, and the fourth electromagnetic valve 41 can control the opening and closing of the air inlet pipe 42, so as to control the aeration to be carried out and stopped. And one end of the bottom aeration component 43 is connected with the air inlet pipe 42, the other end of the bottom aeration component 43 is connected with the material supplementing pipe 22, and the two ends are connected to realize the fixation of the bottom aeration component 43, so that the bottom aeration component 43 is prevented from swinging along with the stirring of water flow, the stability of the bottom aeration device 4 in operation is ensured, and the bottom aeration device is prevented from being broken due to swinging.
As shown in fig. 1, fig. 4 and fig. 5, the bottom aeration assembly 43 of the present invention comprises a first branch aeration pipe 431, a second branch aeration pipe 432, a third branch aeration pipe 433, a fourth branch aeration pipe 434 and a cross four-way joint, wherein one end of the first branch aeration pipe 431 is communicated with an air inlet pipe 42, the other end of the first branch aeration pipe 431 is communicated with the cross four-way joint, the second branch aeration pipe 432, one ends of the third branch aeration pipe 433 and the fourth branch aeration pipe 434 are respectively communicated with the cross four-way joint, the second branch aeration pipe 432, the third branch aeration pipe 433 is sealed with the other end of the fourth branch aeration pipe 434, and one end of the second branch aeration pipe 432 far away from the cross four-way joint is detachably connected with a feed supplement pipe 22. Bottom aeration subassembly 43 is provided with four aeration branch pipes, can carry out abundant aeration to the culture solution, and four aeration branch pipes pass through cross four way connection intercommunication moreover, and four aeration branch pipes aerate to four different directions, make the aeration even. Specifically, the air pump 332 inputs air into the first branch aerator pipe 431 through the air inlet pipe 42, the other end of the first branch aerator pipe 431 is communicated with the cross four-way joint, and the air respectively enters the second branch aerator pipe 432, the third branch aerator pipe 433 and the fourth branch aerator pipe 434 through the cross four-way joint, specifically, the first branch aerator pipe 431, the second branch aerator pipe 432, the third branch aerator pipe 433 and the fourth branch aerator pipe 434 are provided with aeration holes towards one side of the bottom of the fermentation tank 1, namely, the aeration holes are arranged downwards, so that sludge is prevented from precipitating in the aeration holes, the aeration holes are prevented from being blocked, and the air overflows the branch aerator pipe through the aeration holes to form bubbles to aerate the culture solution.
As shown in figure 1 and figure 2, the utility model discloses a biomass generator is provided with the electrode subassembly that is used for responding to the interior liquid level of fermentation cylinder 1, electrode subassembly includes probe 5 under the liquid level electrode, on the liquid level electrode probe 6 and common electrode probe 7, and probe 6 and common electrode probe 7 all are connected with feed liquor diaphragm pump 23 electricity under the liquid level electrode probe 5, the liquid level electrode. Common electrode probe 7 forms the response electrode with probe 5 under the liquid level electrode and probe 6 on the liquid level electrode respectively, and when the liquid level was less than probe 5 under the liquid level electrode in fermentation cylinder 1, feed liquor diaphragm pump 23 started, carries the culture solution in to fermentation cylinder 1, and when the liquid level was higher than probe 6 on the liquid level electrode in fermentation cylinder 1, feed liquor diaphragm pump 23 closed, and the feeding stops.
It should be finally noted that the above embodiments are only intended to illustrate the technical solutions of the present invention, and not to limit the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. A biological generator for nitrobacteria culture is characterized in that: the device comprises a fermentation tank, a material supplementing device for supplementing materials to the fermentation tank and a suction filtration device for suction filtration of culture solution in the fermentation tank, wherein the suction filtration device comprises a hollow fiber membrane component, a liquid pumping control component connected with the hollow fiber membrane component, a backwashing component for backwashing the interior of the hollow fiber membrane component and an external washing component for washing the outer side of the hollow fiber membrane component.
2. The biomass generator for nitrobacteria culture according to claim 1, wherein: the liquid pumping control assembly comprises a liquid outlet diaphragm pump connected with the hollow fiber membrane assembly and a first electromagnetic valve used for controlling the opening and closing between the liquid outlet diaphragm pump and the hollow fiber membrane assembly.
3. The biomass generator for nitrobacteria culture according to claim 1, wherein: the back washing component comprises an air pump connected with the hollow fiber membrane component and a second electromagnetic valve used for controlling the opening and closing of the air pump and the hollow fiber membrane component.
4. The biomass generator for nitrobacteria culture according to claim 3, wherein: the outer washing component comprises an ultrafiltration membrane aeration pipe used for washing the outer side of the hollow fiber membrane component and a third electromagnetic valve connected with the ultrafiltration membrane aeration pipe, and the other end of the third electromagnetic valve is connected with an air pump.
5. The biomass generator for nitrobacteria culture according to claim 4, wherein: one end of the ultrafiltration membrane aeration pipe is communicated with the third electromagnetic valve, the other end of the ultrafiltration membrane aeration pipe extends to the bottom of the hollow fiber membrane component, and a small hole is formed in the side wall of the end.
6. The biomass generator for nitrobacteria culture according to claim 3, wherein: the feed supplement device comprises a feed supplement barrel, a feed supplement pipe and a liquid inlet diaphragm pump for conveying feed liquid in the feed supplement barrel to the fermentation tank, wherein one end of the feed supplement pipe extends to the bottom of the feed supplement barrel, and the other end of the feed supplement pipe extends to the bottom of the fermentation tank.
7. The biomass generator for nitrobacteria culture according to claim 6, wherein: the biomass generator is characterized in that the biomass generator is further provided with an aeration device, the aeration device comprises a fourth electromagnetic valve, an air inlet pipe and a bottom aeration assembly, one end of the air inlet pipe is connected with an air pump, the other end of the air inlet pipe extends into the fermentation tank to be connected with the bottom aeration assembly, the fourth electromagnetic valve is used for controlling the opening and closing of the air inlet pipe, and one end, far away from the air inlet pipe, of the bottom aeration assembly is detachably connected with the feed supplementing.
8. The biomass generator for nitrobacteria culture according to claim 7, wherein: bottom aeration subassembly includes first aeration branch pipe, second aeration branch pipe, third aeration branch pipe, fourth aeration branch pipe and cross four-way connection, first aeration branch pipe one end and intake pipe intercommunication, the other end and the cross four-way connection intercommunication of first aeration branch pipe, the one end of second aeration branch pipe, third aeration branch pipe and fourth aeration branch pipe communicates with the cross four respectively, the other end of second aeration branch pipe, third aeration branch pipe and fourth aeration branch pipe seals the setting, the one end that cross four-way connection was kept away from to the second aeration branch pipe can be dismantled with the feed supplement pipe and be connected, first aeration branch pipe, second aeration branch pipe, third aeration branch pipe, fourth aeration branch pipe are provided with the aeration hole towards the bottom of fermentation cylinder.
9. The biomass generator for nitrobacteria culture according to claim 6, wherein: the biomass generator is provided with an electrode assembly used for sensing the liquid level in the fermentation tank, the electrode assembly comprises a liquid level electrode lower probe, a liquid level electrode upper probe and a common electrode probe, and the liquid level electrode lower probe, the liquid level electrode upper probe and the common electrode probe are all electrically connected with the liquid inlet diaphragm pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020497864.XU CN212293544U (en) | 2020-04-07 | 2020-04-07 | Bio-generator for nitrobacteria culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020497864.XU CN212293544U (en) | 2020-04-07 | 2020-04-07 | Bio-generator for nitrobacteria culture |
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| Publication Number | Publication Date |
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| CN212293544U true CN212293544U (en) | 2021-01-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202020497864.XU Active CN212293544U (en) | 2020-04-07 | 2020-04-07 | Bio-generator for nitrobacteria culture |
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| CN (1) | CN212293544U (en) |
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2020
- 2020-04-07 CN CN202020497864.XU patent/CN212293544U/en active Active
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