CN211999706U - Quantitative excrement sample collecting device - Google Patents
Quantitative excrement sample collecting device Download PDFInfo
- Publication number
- CN211999706U CN211999706U CN201922271958.5U CN201922271958U CN211999706U CN 211999706 U CN211999706 U CN 211999706U CN 201922271958 U CN201922271958 U CN 201922271958U CN 211999706 U CN211999706 U CN 211999706U
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- China
- Prior art keywords
- piston handle
- head
- centrifuge tube
- collecting
- collection
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- 230000002550 fecal Effects 0.000 claims description 18
- 210000000088 Lip Anatomy 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000005070 sampling Methods 0.000 abstract description 13
- 210000002700 Urine Anatomy 0.000 abstract description 5
- 238000011002 quantification Methods 0.000 abstract description 2
- 210000003128 Head Anatomy 0.000 description 47
- 150000007523 nucleic acids Chemical class 0.000 description 11
- 108020004707 nucleic acids Proteins 0.000 description 11
- 238000001514 detection method Methods 0.000 description 5
- 239000003761 preservation solution Substances 0.000 description 5
- 239000012530 fluid Substances 0.000 description 4
- 238000003825 pressing Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 2
- 244000052616 bacterial pathogens Species 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000003608 Feces Anatomy 0.000 description 1
- 208000007906 Intestinal Disease Diseases 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 201000011231 colorectal cancer Diseases 0.000 description 1
- 230000004059 degradation Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000000968 intestinal Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic Effects 0.000 description 1
- 230000000630 rising Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Images
Abstract
The utility model discloses a quantitative excrement and urine sample collection system, include: the piston comprises a first piston handle, a second piston handle, an empty cylinder, a collecting head and a centrifuge tube; the second piston handle is sleeved on the first piston handle, and the second piston handle is arranged in the hollow cylinder; the tail end of the first piston handle is connected with a collecting head; the empty cylinder is matched with the centrifugal tube. The collecting device is simple and easy to operate, both thin and thick excrement and urine can be collected, repeated sampling is avoided in one-time definite quantification, and the collecting method is sanitary and easy to accept.
Description
Technical Field
The utility model belongs to the technical field of the medical instrument technique and specifically relates to a quantitative collection system of excrement and urine sample is related to.
Background
With the improvement of medical science and the improvement of detection technology, some intestinal diseases are detected by extracting human-derived, intestinal bacteria or virus nucleic acid substances from excrement for assay analysis.
When excrement nucleic acid is extracted, excrement sampling equipment is used, and a sample is collected and then placed into a treatment fluid test tube to prepare for subsequent detection. In many methods for extracting nucleic acid from excrement, quantitative excrement samples are required to be added due to the limitation of reagent amount, excrement sampling equipment in the market at present has the forms of sampling spoons, sampling rods and the like, and some excrement sampling equipment can achieve uniform sampling for many times, but the actual weight is not very clear, and repeated sampling is needed when the sample amount is insufficient sometimes, and the special characteristics of the detected materials easily cause the psychological conflict of samplers. When the collected sample is filled into the treatment fluid, due to different fecal viscosities, some fecal is easily adhered to the collector and is not easy to separate, the uniform mixing degree of the filled sample and the treatment fluid cannot be ensured, if the nucleic acid is not sufficiently mixed, the nucleic acid is easily degraded, the extraction quality of the nucleic acid at the later stage is influenced, and if the fecal sample needs to be stored for a short time, pathogenic microorganisms contained in the fecal are easily fermented or polluted, and the morphological odor of the pathogenic microorganisms easily causes the conflict psychology of experimenters at the next step.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a fecal sample ration collection system.
To achieve the above object, the present invention adopts the following:
a fecal specimen quantitative collection device comprising: the piston comprises a first piston handle, a second piston handle, an empty cylinder, a collecting head and a centrifuge tube; the second piston handle is sleeved on the first piston handle, and the second piston handle is arranged in the hollow cylinder; the tail end of the first piston handle is connected with a collecting head; the centrifuge tube is matched with the empty cylinder.
Preferably, the collection head comprises a collection head stem, a collection head neck and a collection head flap; the collecting head stem is connected with the first piston handle through a clamping piece; the collection head and neck is connected with the collection head stalk; the collection head and neck are in a herringbone shape, and the two arms are connected with a collection head flap; the two collecting head petals are both hemispherical and are spliced into a sphere.
The sphere formed by splicing the two collecting head valves is a collecting cavity, the capacity of the collecting cavity is certain, and the collecting cavities with different capacities can be selected according to different requirements.
Preferably, the centrifuge tube comprises an inner centrifuge tube, an outer centrifuge tube and a tube cap; the inner centrifuge tube is matched with the empty barrel; an outward lip protrusion is arranged at the pipe orifice of the inner centrifugal pipe; the lip convex part is matched with the outer centrifuge tube; and a pipe cover is arranged at the pipe opening of the outer centrifugal pipe.
Preferably, the second piston handle and the hollow cylinder are connected through threads. So that the second piston handle can rotate up and down, the collecting head flap can rotate, and the aim of rotating and stirring in the centrifugal tube is fulfilled.
Preferably, the tail end of the first piston handle is provided with a U-shaped buckle, and the inner edge of the buckle is provided with two anti-falling lugs; the upper end of the collecting head stem is provided with an anti-falling pit and matched with the anti-falling lug.
Preferably, the collection neck is made of an elastic material. The daily state remains in a chevron shape.
The utility model has the advantages of it is following:
1. the device can fully and uniformly mix the excrement sample and the treatment fluid, and can further effectively separate sample residues. Accurately quantifying the fecal sample, and definitely counting the concentration of certain bacteria or viruses according to the detection result to provide accurate data for disease treatment; the automatic mixing function facilitates the operation process of sampling personnel, reduces the possibility of degradation of nucleic acid substances, and simultaneously can remove residues of the excrement sample, thereby facilitating the next inspection or storage, providing convenience for the work of inspection personnel and improving the acceptance of work content.
2. The collecting device is simple and easy to operate, both thin and thick excrement and urine can be collected, repeated sampling is avoided in one-time definite quantification, and the collecting method is sanitary and easy to accept.
Drawings
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
Fig. 1 is a schematic structural view of a quantitative fecal sample collecting device of the present invention.
Fig. 2 is a schematic view of a partial enlarged structure of a quantitative fecal specimen collecting device of the present invention.
FIG. 3 is a schematic view of the structure of the centrifugal tube of the device for quantitatively collecting the stool sample
Fig. 4 is a schematic structural view of a collecting head of a quantitative fecal sample collecting device of the present invention.
In the figures, the various reference numbers are:
1-first piston handle, 2-second piston handle, 3-empty cylinder, 4-collecting head, 41-collecting head stem, 42-collecting head neck, 43-collecting head flap, 5-centrifuge tube, 51-inner centrifuge tube, 511-lip protrusion, 52-outer centrifuge tube, 53-tube cover, 6-anti-falling bump and 7-anti-falling pit.
Detailed Description
In order to illustrate the invention more clearly, the invention is further described below with reference to preferred embodiments. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "front", "rear", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected" and "disposed" are to be construed broadly, and may for example be fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1 to 4, a quantitative fecal specimen collecting apparatus includes: a first piston handle 1, a second piston handle 2, an empty cylinder 3, a collection head 4 and a centrifuge tube 5; the collecting head 4 comprises a collecting head stem 41, a collecting head neck 42 and a collecting head flap 43; the centrifuge tube 5 includes an inner centrifuge tube 51, an outer centrifuge tube 52, and a tube cap 53.
The first piston handle 1 is inserted into the second piston handle 2, and the first piston handle 1 and the second piston handle 2 slide in the vertical direction relatively and cannot rotate relatively.
It should be noted that, the connecting structure meeting the above-mentioned motion mode may be provided with a sliding groove and a sliding block between the first piston handle 1 and the second piston handle 2, or the first piston handle 1 is provided with an oval shape.
Further, No. two piston handles 2 and the hollow cylinder 3 adopt threaded connection between for No. two piston handles 2 can make spiral rising and spiral decline, thereby drive No. one piston handle 1 and make same motion.
Further, the lower end of the first piston handle 1 is provided with a U-shaped buckle, two anti-falling lugs 6 are arranged on the inner edge of the buckle, and an anti-falling pit 7 is arranged at the upper end of the collecting head stem 41 and is clamped with the anti-falling lugs 6. The anti-drop convex block 6 and the anti-drop concave pit 7 can be separated by pulling forcefully, so that the collecting heads 4 with different capacities can be replaced conveniently.
Further, the collection head neck 42 is connected with the collection head stem 41; the collection head neck 42 is in a herringbone shape, and the two arms are both connected with a collection head flap 43; the two collecting head flaps 43 are both hemispherical and are spliced into a sphere.
The connection mode of the acquisition head stem 41 and the acquisition head neck 42 is fixed connection, which may be glue bonding, heat welding, threaded connection, etc., and this utility model adopts a glue bonding connection mode. The collection head neck 42 is kept in a herringbone open state, and when the first piston handle 1 is pulled upwards, the hollow cylinder 3 can drive the collection head neck 42 to be closed, so that the two collection head flaps 43 form a spherical collection cavity. The collection neck 42 needs to have high elasticity and is therefore made of an elastic material.
Further, the mouth of pipe department of interior centrifuging tube 51 is provided with outside lip convex part 511, puts into outer centrifuging tube 52 with interior centrifuging tube 51, lip convex part 511 just blocks the opening at outer centrifuging tube 52, and the mouth of pipe outside of outer centrifuging tube 52 still is connected with tube cap 53 for seal centrifuging tube 5, wherein the bottom of interior centrifuging tube 51 is provided with inner core filtration membrane, and inner core filtration membrane can filter the material that the detection is not required, and when abandoning interior centrifuging tube 51, can abandon the residue material.
The working principle of the device is as follows: according to the experiment requirement, a collecting head 4 with a proper model is selected and forcibly inserted into the hollow cylinder 3, so that the anti-falling convex block 6 and the anti-falling concave pit 7 are buckled.
And lifting the first piston handle 1 to drive the sampling head 4 to be combined, and collecting the excrement sample while combining.
The collection head 4 is inserted into the inner centrifuge tube 51, the first piston handle 1 is pushed to restore the original position, and the excrement nucleic acid preservation solution or the extracting solution is added into the inner centrifuge tube 51.
Pressing, carrying No. two piston handles 2, the screw thread rotation drives the clockwise, anticlockwise rotation of collection head 4, and collection head lamella 43 plays fine stirring function for excrement and urine sample and nucleic acid preserve solution or extract are abundant to mix.
Pressing the first piston handle 1, the collecting head 4 falls off, and the collecting head 4 is discarded.
Centrifuge tube lid 53 is closed and centrifugation is performed, with liquid entering outer centrifuge tube 52 and fecal debris in inner centrifuge tube 51.
After centrifugation, the inner centrifuge tube 51 is discarded after opening the lid.
And (4) covering the tube cover, and storing the sample or performing the next nucleic acid extraction experiment.
The following example of operation:
colorectal cancer screening experiment requirements are as follows: 150mg of feces are taken today and suspended in 1ml of preservation solution for tomorrow detection assay.
1. According to the experimental requirements, a collecting head (150mg sampling amount) with the diameter of 5mm is selected and forcibly inserted into the empty cylinder, so that the anti-skid lugs and the anti-skid pits are buckled.
2. And drawing a first piston handle to drive the sampling head to be combined, and collecting 150mg of the excrement sample while combining.
3. 1ml of preservation solution is added into the inner centrifuge tube for standby.
4. And (3) pushing the first piston handle to restore the original position, and inserting the collection head into the preservation solution of the inner core of the centrifugal tube.
5. And pressing and lifting the second piston handle, rotating the collecting head clockwise and anticlockwise, and stopping operation after the excrement sample and the nucleic acid preservation solution are fully and uniformly mixed.
6. Pressing the first piston handle, and discarding the collecting head.
7. And covering a centrifugal tube cover, centrifuging for 5min at 10000rpm of a centrifugal machine, and taking out the centrifugal tube.
8. And after the cover is opened, taking out the inner centrifugal tube and discarding.
And covering a tube cover, putting the centrifuge tube into a refrigerator at 4 ℃ for storage, and detecting and testing the centrifuge tube in the next day.
Obviously, the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it is obvious for those skilled in the art to make other variations or changes based on the above descriptions, and all the embodiments cannot be exhausted here, and all the obvious variations or changes that belong to the technical solutions of the present invention are still in the protection scope of the present invention.
Claims (7)
1. A quantitative fecal sample collection device comprising: the piston comprises a first piston handle, a second piston handle, an empty cylinder, a collecting head and a centrifuge tube; the second piston handle is sleeved on the first piston handle, and the second piston handle is arranged in the hollow cylinder; the tail end of the first piston handle is connected with a collecting head; the centrifuge tube is matched with the empty cylinder.
2. The quantitative fecal sample collection device of claim 1 wherein the collection head comprises a collection head stem, a collection head neck and a collection head flap; the collecting head stem is connected with the first piston handle through a clamping piece; the collection head and neck is connected with the collection head stalk; the collection head and neck are in a herringbone shape, and the two arms are connected with a collection head flap; the two collecting head petals are both hemispherical and are spliced into a sphere.
3. The quantitative fecal sample collection device of claim 1 wherein the centrifuge tube comprises an inner centrifuge tube, an outer centrifuge tube and a tube cap; the inner centrifuge tube is matched with the empty barrel; an outward lip protrusion is arranged at the pipe orifice of the inner centrifugal pipe; the lip convex part is matched with the outer centrifuge tube; and a pipe cover is arranged at the pipe opening of the outer centrifugal pipe.
4. The quantitative fecal sample collecting device according to claim 1 characterized in that the second piston handle and the hollow cylinder are connected by screw thread.
5. The quantitative excrement sample collecting device according to claim 2, wherein a U-shaped buckle is arranged at the tail end of the first piston handle, and two anti-falling lugs are arranged on the inner edge of the buckle; the upper end of the collecting head stem is provided with an anti-falling pit and matched with the anti-falling lug.
6. The quantitative fecal sample collection device of claim 2 wherein the collection neck is made of an elastic material.
7. The quantitative fecal sample collecting device according to claim 3, wherein the lower end of the inner centrifuge tube is provided with an inner core filtering membrane.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201922271958.5U CN211999706U (en) | 2019-12-18 | 2019-12-18 | Quantitative excrement sample collecting device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201922271958.5U CN211999706U (en) | 2019-12-18 | 2019-12-18 | Quantitative excrement sample collecting device |
Publications (1)
Publication Number | Publication Date |
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CN211999706U true CN211999706U (en) | 2020-11-24 |
Family
ID=73408374
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201922271958.5U Active CN211999706U (en) | 2019-12-18 | 2019-12-18 | Quantitative excrement sample collecting device |
Country Status (1)
Country | Link |
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CN (1) | CN211999706U (en) |
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2019
- 2019-12-18 CN CN201922271958.5U patent/CN211999706U/en active Active
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