CN211814494U

CN211814494U - Multifunctional cell culture device

Info

Publication number: CN211814494U
Application number: CN202020049725.0U
Authority: CN
Inventors: 叶婷; 李芳芳; 周梅; 张翔; 李�灿
Original assignee: Guiyang University
Current assignee: Guiyang University
Priority date: 2020-01-07
Filing date: 2020-01-07
Publication date: 2020-10-30
Anticipated expiration: 2030-01-07

Abstract

The utility model discloses a multi-functional cell culture device, including body, cultivation layer, handle, barrel and scraper blade, the body is the square body, is equipped with the handle in one side of body, is equipped with the square hole at the body lateral wall that does not interfere with the handle, is equipped with the layer board in the bottom of square hole, cultivates the layer dress on the layer board, and with the cooperation of square hole, the barrel slope is established in one side of body, and the barrel is centrum form, and the main aspects of barrel up is equipped with the end cover in the entrance of barrel, the scraper blade dress is on the screens that the body inboard was equipped with. The utility model can make full use of the internal structure of the culture device, can directly brush off cells by using the scraper, can directly add cell culture solution into the bottle, and reduces the risk of pollution in the cell culture process; the adherent growth condition of the cells is not needed to be worried about, the survival rate of the cells is improved, and the experimental operation time is saved.

Description

Multifunctional cell culture device

Technical Field

The utility model relates to a laboratory cell culture utensil field relates to a multi-functional cell culture device.

Background

A common cell culture device is used for culturing a few cells in a laboratory, and is used for transferring the cells to a pore plate for experiment after the adherent growth of the cells reaches the required density by a temporary culture device of the cells when the cells are subjected to experimental toxicity test, protein influence determination and passage cryopreservation for cell preservation. In the process of passage treatment and cell transfer, the cells are separated from the bottom of the culture device by using a cell scraper when the cells are transferred, the operation steps of the process are complex, mixed bacteria are easily introduced, and the risk of cell pollution is increased.

Cell density is cultivateed, need to change the cell culture solution in-process, and the cell culture solution need be squeezed into so as not to influence adherent cell growth from the lateral wall, and the cell is resuspended in order to distribute evenly behind the blake bottle, and the laboratory technician can rock the blake bottle, but the cell receives centrifugal force effect like this and pastes peripherally, makes the cell flow in the bottle wall, reduces cell survival rate. Meanwhile, the traditional cell bottle has a small number of cells cultured in a single layer, is not beneficial to large-scale experiments, and wastes culture bottle materials.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a multifunctional cell culture device.

The utility model discloses a following technical scheme can realize.

The utility model provides a pair of multi-functional cell culture device, including body, cultivation layer, handle, barrel and scraper blade, the body is the square body, is equipped with the handle in one side of body, is equipped with the square hole at the body lateral wall that does not interfere with the handle, is equipped with the layer board in the bottom of square hole, cultivates the layer dress on the layer board, and cooperates with the square hole, the barrel slope is established in one side of body, and the barrel is centrum form, and the main aspects of barrel up is equipped with the end cover in the entrance of barrel, the scraper blade is adorned on the screens that the body inboard was equipped with.

The body is a square shell with an uncovered upper end.

The three layers of culture layers are arranged, surrounding edges are arranged on the peripheries of the culture layers, and handles are further arranged on one sides of the surrounding edges.

The gun barrel is three in number, is obliquely arranged on the side wall of the body and corresponds to the three culture layers, and the muzzle of the gun barrel is higher than the upper surfaces of the corresponding culture layers.

The scraper blade is rectangular form, and the upper portion of scraper blade is equipped with the bar decorative pattern form, and lower part and bottom are smooth and level.

The beneficial effects of the utility model reside in that: the utility model can make full use of the internal structure of the culture device, can directly brush off cells by using the scraper, can directly add cell culture solution into the bottle, and reduces the risk of pollution in the cell culture process; the adherent growth condition of the cells is not needed to be worried about, the survival rate of the cells is improved, and the experimental operation time is saved.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a schematic view of a structure without a culture layer;

fig. 3 is a partial schematic view of the present invention.

Detailed Description

The technical solution of the present invention is further described below with reference to the accompanying drawings, but the scope of protection claimed is not limited to the description.

As shown in fig. 1, fig. 2 and fig. 3, the utility model provides a pair of multi-functional cell culture device, including body 1, cultivation layer 2, handle 3, barrel 4 and scraper blade 5, body 1 is the square body, is equipped with handle 3 in one side of body 1, is equipped with square hole 11 at the body 1 lateral wall that does not interfere with handle 3, is equipped with layer board 12 in the bottom of square hole 11, cultivates layer 2 dress on layer board 12, and with the cooperation of square hole 11, barrel 4 slope is established in one side of body 1, and barrel 4 is the cone form, and the main aspects of barrel 4 up is equipped with the end cover in the entrance of barrel 4, scraper blade 5 dress is on the screens 13 that body 1 inboard was equipped with.

The body 1 is a square shell with an uncovered upper end, and the square body corresponds to the square hole 11.

Cultivate layer 2 three layers altogether, the periphery of cultivating layer 2 is equipped with the surrounding edge, and one side of surrounding edge still is equipped with handle 21, increases the area of cultivateing the cell, cultivates layer 2 simultaneously and can dismantle, is convenient for wash.

The gun barrel 4 is three in number, is obliquely arranged on the side wall of the body 1 and corresponds to the three culture layers 2, and the muzzle position of the gun barrel 4 is higher than the upper surface of the corresponding culture layer 2. The gun tube 4 is specially used for adding and sucking and discarding cell culture media, and can effectively prevent pollution.

Two screens 13 are designed on the culture layer 2 on the inner side of the body 1, the height of each screen 13 is higher than that of the upper surface of the culture layer 2, and each screen 13 is used for storing the scraper 5.

The scraper 5 is strip-shaped, the upper part of the scraper 5 is provided with strip-shaped patterns, and the lower part and the bottom are smooth and flat. The scraping plate 5 is provided with stripes which can increase friction force when being held by hand, and the bottom is a smooth scraping head. This scraper blade 5 both can be used to the culture solution drainage, can have the effect that the cell scraped again, has avoided the inconvenience in the aspect of single somatic cell scraping clearance, depositing etc. to reach the experiment purpose simultaneously.

Claims

1. The utility model provides a multi-functional cell culture device, its characterized in that, includes body (1), cultivates layer (2), handle (3), barrel (4) and scraper blade (5), body (1) is the square body, is equipped with handle (3) in one side of body (1), is equipped with square hole (11) at body (1) lateral wall that does not interfere with handle (3), is equipped with layer board (12) in the bottom of square hole (11), cultivates layer (2) dress on layer board (12), and with square hole (11) cooperation, barrel (4) slope is established in one side of body (1), and barrel (4) are the cone form, and the main aspects of barrel (4) are up, are equipped with the end cover at the entrance of barrel (4), scraper blade (5) dress is on screens (13) that body (1) inboard is equipped with.

2. The multipurpose cell culture device according to claim 1, wherein the body (1) is a square housing with no cover at the upper end.

3. The multifunctional cell culture device according to claim 1, wherein the culture layer (2) has three layers, the periphery of the culture layer (2) is provided with a surrounding edge, and one side of the surrounding edge is further provided with a handle (21).

4. The multifunctional cell culture device according to claim 1, wherein the number of the gun tubes (4) is three, the three gun tubes are respectively arranged on the side wall of the body (1) in an inclined manner and correspond to the three culture layers (2), and the muzzle of the gun tube (4) is higher than the upper surface of the corresponding culture layer (2).

5. The multi-functional cell culture device according to claim 1, wherein the scraper (5) is in the shape of a long bar, the upper part of the scraper (5) is provided with a rib shape, and the lower part and the bottom part are smooth and flat.