Centrifugal tank for biological agent
Technical Field
The utility model relates to a centrifugation jar technical field specifically is a centrifugation jar for biological agent.
Background
In biological tests, in order to separate specific components required in a target liquid, a centrifugal tank is generally required and a density gradient centrifugal separation method is adopted, wherein the method utilizes the difference of the densities of different components in the target liquid, and the different components are radially layered during high-speed centrifugation to separate and extract the components. Traditional centrifugation extraction need use the centrifuging tube and after centrifuge stops rotating, extracts required component, and is often inefficiency, needs a large amount of manual loaded down with trivial details operations.
SUMMERY OF THE UTILITY MODEL
The utility model discloses to the technical problem that will solve, provide a centrifugal tank that is used for biological agent can realize realizing centrifugal target component extraction under high flux, the high-speed rotation.
In order to solve the technical problem, the utility model discloses a realize through following technical scheme: a centrifugation tank for a biological agent, comprising a tank cover and a tank body which are fitted to each other, and a central shaft which penetrates into the inside of the tank cover, wherein,
the tank cover is internally provided with a first material inlet/outlet channel, one end of the first material inlet/outlet channel is communicated with the central shaft, and the other end of the first material inlet/outlet channel is connected to the inner cavity of the tank body;
the tank body is of a hollow structure, the center of the tank body is provided with an upright post, the edge of the tank body is provided with a material inlet/outlet groove, the bottom of the tank body is provided with a second material inlet/outlet channel, one end of the second material inlet/outlet channel is positioned at an outlet of the material inlet/outlet groove, and the other end of the second material inlet/outlet channel extends along the center of the upright post and is communicated with the central shaft;
the central shaft is internally provided with a first feeding/discharging hole and a second feeding/discharging hole which are parallel and independent, one end of the first feeding/discharging hole is communicated with the first feeding/discharging channel, the other end of the first feeding/discharging hole is connected to an external storage container, one end of the second feeding/discharging hole is communicated with the second feeding/discharging channel, and the other end of the second feeding/discharging hole is connected to the external storage container.
Furthermore, the centrifugal tank further comprises connectors symmetrically arranged on two sides of the top of the central shaft, a bearing sleeved on the outer side of the middle section of the central shaft, and a movable sealing ring sleeved on the outer side of the bottom of the central shaft.
Furthermore, the movable sealing rings are grouped sealing rings and distributed on the upper side and the lower side of the first material inlet/outlet channel.
Furthermore, joint holes are symmetrically formed in the top of the central shaft and used for installing the joints.
Furthermore, the tank body comprises an upper tank body used for realizing biological agent separation and a lower tank body connected to the power device, the lower tank body is provided with a shaft hole used for being matched with a power shaft of the power device, and the outer side of the shaft hole is provided with a fastening hole used for fixing the power device and the lower tank body and a positioning hole used for mounting and positioning.
Furthermore, an O-shaped sealing ring is arranged at the joint of the upright post and the tank cover.
Furthermore, a buffer groove is formed in the middle of the tank cover and is located at the connecting position of the first material inlet/outlet channel and the first material inlet/outlet hole.
Furthermore, the top of the tank cover is provided with a light path notch for passing through a light path during an image acquisition experiment.
Furthermore, the excircle of the dynamic seal ring is tightly attached to the inner wall of the tank cover and is fixed, and the inner seal lip is tightly attached to the central shaft and can rotate relatively.
Further, the dynamic sealing ring is a low-friction sealing ring.
Compared with the prior art, the utility model discloses an useful part is:
firstly, the utility model provides a centrifugal tank, through set up first advance/discharge channel on the cover, set up second advance/discharge channel on the jar body, set up first advance/discharge hole and second advance/discharge hole on the center pin, realize the functions such as totally enclosed integration, automatic centrifugation, separation, concentration of the feeding, extraction and the ejection of compact of each component, replaced the use of centrifuging tube in traditional centrifuge, realized fast, high-efficient, large flux, unmanned operation; centrifugal liquid such as biological agents enters the inner cavity of the tank body through the joint through the first inlet/outlet hole and the first inlet/outlet channel, centrifugal layering is achieved under the action of high-speed rotating centrifugal force, layered target components are distributed at an inlet/outlet of the second inlet/outlet channel of the tank body, the target components are extracted simultaneously under the conditions of high-speed rotation and high flux of the centrifugal tank, and efficiency is greatly improved.
And the design of a central shaft with a small shaft diameter and a specific dynamic sealing ring ensures that the dynamic sealing does not generate a large amount of heat under the high-speed rotation condition of the centrifugal tank, so that the target components extracted from the second material inlet/outlet channel are heated to change the properties, and simultaneously, the good sealing performance can be ensured, so that the whole centrifugal tank and all the channels thereof are not polluted by the outside.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
The present invention will be further explained with reference to the accompanying drawings:
FIG. 1 is a schematic structural view of a centrifuge tank according to the present invention;
FIG. 2 is a cross-sectional view of FIG. 1;
FIG. 3 is an enlarged view of a portion of FIG. 2;
FIG. 4 is a schematic structural view of the tank of the present invention;
FIG. 5 is a cross-sectional view of FIG. 4;
FIG. 6 is a schematic view of a central shaft according to an embodiment;
FIG. 7 is a cross-sectional view of FIG. 6;
1. a can lid; 2. a tank body; 3. a central shaft; 4. a joint; 5. an optical path notch; 6. a bearing; 7. a movable sealing ring; 8. an O-shaped sealing ring; 11. a first inlet/outlet channel; 12. a buffer tank; 21. feeding the tank body; 22. a tank body is arranged; 23. a fastening hole; 24. a column; 25. an inlet/outlet chute; 26. a second inlet/outlet channel; 27. positioning holes; 31. a first inlet/outlet hole; 32. a second inlet/outlet hole; 33. a connector bore.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present application, unless expressly stated or limited otherwise, the terms "connected" and "fixed" are to be construed broadly, e.g., "fixed" may be fixedly connected or detachably connected, or integrally formed; the connection can be mechanical connection, electrical connection, physical connection or wireless communication connection; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In addition, the technical solutions between the embodiments of the present invention can be combined with each other, but it is necessary to be able to be realized by a person having ordinary skill in the art as a basis, and when the technical solutions are contradictory or cannot be realized, the combination of such technical solutions should be considered to be absent, and is not within the protection scope of the present invention.
A centrifugal tank for biological agents as shown in figures 1-3, comprising a tank cover 1 and a tank body 2 which are matched with each other, and a central shaft 3 which penetrates into the tank cover 1, wherein the inner circle of the tank cover 1 is tightly attached to the outer circle of the tank body 2, and the central shaft 3 is coincident with the geometric centers of the tank cover 1 and the tank body 2.
A first material inlet/outlet channel 11 for injecting/extracting centrifugal liquid such as biological agents is formed in the tank cover 1, one end of the first material inlet/outlet channel 11 is communicated with the central shaft 3, and the other end of the first material inlet/outlet channel is connected to the inner cavity of the tank body 2; when injection is needed, centrifugal liquid such as biological agents enters the inner cavity of the tank body 2 through the joint 4 through the first inlet/outlet hole 31 and the first inlet/outlet channel 11; when extraction is required, extraction is reversed from the connector 4.
As shown in fig. 4 and 5, the tank body 2 has a hollow structure inside, a column 24 is arranged at the center, an inlet/outlet chute 25 for guiding liquid is arranged at the edge, a second inlet/outlet channel 26 is arranged at the bottom, one end of the second inlet/outlet channel 26 is positioned at the outlet of the inlet/outlet chute 25, and the other end extends along the center of the column 24 and is communicated with the central shaft 3; the second material inlet/outlet channel 26 and the first material inlet/outlet channel 11 are L-shaped integrally, and an O-shaped sealing ring 8 is arranged at the joint of the upright post 24 and the tank cover 1, so that centrifugal liquids such as biological agents in the tank body 2 are prevented from entering the central shaft 3.
As shown in fig. 6 and 7, the central shaft 3 is provided therein with a first inlet/outlet hole 31 and a second inlet/outlet hole 32 which are parallel and independent from each other, one end of the first inlet/outlet hole 31 is communicated with the first inlet/outlet channel 11, the other end is connected to an external storage container, one end of the second inlet/outlet hole 32 is communicated with the second inlet/outlet channel 26, and the other end is connected to the external storage container; centrifugal liquid such as biological agents enters the tank body 2 through the first inlet/outlet hole 31 and/or the joint 4, centrifugal layering is achieved under the action of high-speed rotating centrifugal force, layered target components are distributed at an inlet/outlet of the second inlet/outlet channel 26 of the tank body 2, the target components are extracted simultaneously under the conditions of high-speed rotation and high flux, and efficiency is greatly improved.
Preferably, the centrifugal tank further comprises connectors 4 symmetrically arranged on two sides of the top of the central shaft 3, a bearing 6 sleeved on the outer side of the middle section of the central shaft 3, and a dynamic seal ring 7 sleeved on the outer side of the bottom of the central shaft 3; in this embodiment, the dynamic seal ring 7 is a low friction seal ring, is used in groups, and is distributed on the upper side and the lower side of the first inlet/outlet channel 11, and the lip design and the fit interference magnitude of the special material and optimization ensure good sealing performance, and simultaneously avoid temperature rise caused by high-speed friction between the sealing lip and the central shaft.
The first material inlet/outlet channel 11 arranged on the tank cover 1, the second material inlet/outlet channel 26 arranged on the tank body 2 and the first material inlet/outlet hole 31 and the second material inlet/outlet hole 32 arranged on the central shaft 3 replace the use of a centrifugal tube in the traditional centrifuge, and the rapid and efficient automatic operation is realized.
Preferably, the tank body 2 comprises an upper tank body 21 for realizing biological agent separation and a lower tank body 22 connected to the power device, the lower tank body 22 is provided with a shaft hole for matching with a power shaft of the power device, and the outer side of the shaft hole is provided with a fastening hole 23 for fixing the power device and the lower tank body 22 and a positioning hole 27 for mounting and positioning; more specifically, the power device is a servo motor, and can directly drive the tank body 2 to rotate after being positioned by the positioning hole 27 and fixedly connected with the centrifugal tank by the fastening hole 23.
Preferably, as shown in fig. 3, a buffer tank 12 is provided at a connection position of the first inlet/outlet channel 11 and the first inlet/outlet hole 31 in the middle of the tank cover 1, centrifugal liquid such as biological agent enters from the joint 4, enters the buffer tank 12 through the first inlet/outlet hole 31, and then enters the first inlet/outlet channel 11 from the buffer tank 12, during the rotation process, the tank cover 1 and the tank body 2 rotate synchronously, the central shaft 3 remains stationary, and the buffer tank 12 is designed to ensure that the centrifugal liquid such as biological agent smoothly enters the first inlet/outlet channel 11 without being influenced by the rotation; as shown in fig. 1, the top of the tank cover 1 is provided with a light path notch 5 for passing a light path when performing an image acquisition experiment.
Preferably, the top of the central shaft 3 is symmetrically provided with joint holes 33 for installing joints 4, the joints 4 are luer joints, are standardized micro non-seepage joints, and belong to the prior art.
As preferred, the movable seal circle excircle is hugged closely the inner wall of cover 1 is hugged closely fixedly, the inner seal lip center pin 3 and can rotate relatively, and movable seal circle cooperates with the center pin 3 of tiny footpath, ensures the utility model discloses a centrifuge tank is under the high-speed rotatory state, and movable seal is unlikely to produce a large amount of heats, leads to the target component of following extraction in second advance/discharging channel 26 to be heated up and take place the nature change, can guarantee good sealing performance simultaneously, is unlikely to make whole centrifuge tank and each passageway by external contamination.
The specific use process of centrifugation jar is: the power shaft of the servo motor is positioned through the positioning hole 27, and the screw is matched with the fastening hole 23 to realize the positioning and the fixing of the utility model and the servo motor; the power supply is started, the tank cover 1, the tank body 2, the bearing 6, the dynamic sealing ring 7 and the O-shaped sealing ring 8 synchronously rotate, the central shaft 3 and the joint 4 are kept static, centrifugal liquid such as biological agents is provided by an external storage container, enters the buffer groove 12 from the joint 4 through the first material inlet/outlet hole 31, enters the inner cavity of the tank body 2 through the first material inlet/outlet channel 11 and the material inlet/outlet groove 25, centrifugal layering is realized under the action of high-speed rotation centrifugal force, the layered target components are distributed at the material inlet/outlet of the second material inlet/outlet channel 26 of the tank body 2, and are extracted to the external storage container through the second material inlet/outlet channel 26 and the second material inlet/outlet hole 32, so that centrifugal separation and extraction are completed.
The utility model provides a first advance/discharging channel 11, advance/discharging tank 25, second advance/discharging channel 26, first advance/discharging hole 31 and second advance/discharging hole 32 can advance the feeding or extract according to the experiment needs of difference.
Example two
A centrifugal can for biological agents as shown in fig. 1-5, comprising a can cover 1 and a can body 2 which are mutually matched and a central shaft 3 which penetrates into the interior of the can cover 1, which is different from the first embodiment: two first material inlet/outlet channels 11 are arranged in the tank cover 1; the edge of the tank body 2 is provided with two feeding/discharging grooves 25, the bottom of the tank body is provided with two second feeding/discharging channels 26, one end of each second feeding/discharging channel 26 is positioned at an outlet of the feeding/discharging groove 25, and the other end of each second feeding/discharging channel 26 extends along the center of the upright column 24 and is communicated with the central shaft 3; two first material inlet/outlet holes 31 and two second material inlet/outlet holes 32 are respectively formed in the central shaft 3, one end of each first material inlet/outlet channel 11 is respectively communicated with the material inlet/outlet groove 25 and the tank body 2 in sequence, the other end of each first material inlet/outlet channel is communicated with one first material inlet/outlet hole 31, one end of each second material inlet/outlet channel 26 is arranged at the bottom of one material inlet/outlet groove 25, and the other end of each second material inlet/outlet channel is communicated with one second material inlet/outlet hole 32.
Compared with the first embodiment, the double arrangement of the first material inlet/outlet channel 11, the material inlet/outlet groove 25, the second material inlet/outlet channel 26, the first material inlet/outlet hole 31 and the second material inlet/outlet hole 32 realizes faster material feeding and extraction, further improves the processing efficiency, and has the same using process and working principle as the first embodiment.
The above only be the preferred embodiment of the utility model discloses a not consequently restriction the utility model discloses a patent range, all are in the utility model discloses a conceive, utilize the equivalent structure transform of what the content was done in the description and the attached drawing, or direct/indirect application all is included in other relevant technical field the utility model discloses a patent protection within range.