CN211460665U - Mouse heart perfusion device - Google Patents
Mouse heart perfusion device Download PDFInfo
- Publication number
- CN211460665U CN211460665U CN201821378338.0U CN201821378338U CN211460665U CN 211460665 U CN211460665 U CN 211460665U CN 201821378338 U CN201821378338 U CN 201821378338U CN 211460665 U CN211460665 U CN 211460665U
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- Prior art keywords
- injection
- blood
- needle
- physiological solution
- perfusion device
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- Expired - Fee Related
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- 230000010412 perfusion Effects 0.000 title claims abstract description 25
- 239000007924 injection Substances 0.000 claims abstract description 87
- 238000002347 injection Methods 0.000 claims abstract description 87
- 239000008280 blood Substances 0.000 claims abstract description 66
- 210000004369 blood Anatomy 0.000 claims abstract description 66
- 238000000605 extraction Methods 0.000 claims abstract description 42
- 239000000243 solution Substances 0.000 claims abstract description 39
- 238000007789 sealing Methods 0.000 claims description 38
- 230000000740 bleeding effect Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 210000005240 left ventricle Anatomy 0.000 abstract description 8
- 210000005245 right atrium Anatomy 0.000 abstract description 6
- 230000004087 circulation Effects 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 4
- 229940079593 drug Drugs 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 239000003918 blood extract Substances 0.000 abstract 1
- 230000001839 systemic circulation Effects 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 13
- 241000699670 Mus sp. Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002837 heart atrium Anatomy 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 210000005241 right ventricle Anatomy 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The utility model discloses a mouse heart perfusion device, this perfusion device collection injection propulsion and blood extract in an organic whole, utilize piston motion and pressure variation, realize that physiological solution's injection and blood's extraction are stored, through the systemic circulation system of heart, have established physiological solution and have replaced closed loop. The push-pull rod is pulled to compress the physiological solution cavity to generate thrust for injecting the physiological solution into the left ventricle, and meanwhile, the blood storage cavity is expanded to generate suction for drawing blood from the right atrium, so that the blood is replaced by the physiological solution, the influence of the blood on the experimental determination of the tissue can be reduced, and the reaction of the tested tissue to environmental factors or drug factors can be reflected more truly. In addition, the perfusion device collects the flowing blood while perfusing physiological solution into the body for circulation, and recovers the whole blood, thereby avoiding the pollution of the blood to the experiment table and being convenient to operate.
Description
Technical Field
The utility model relates to an animal experiment field especially relates to a mouse heart perfusion device.
Background
The replacement of blood in tissues by animal heart perfusion is a common technology in physiological experiments, and can eliminate the influence of blood on the measurement of tissue related indexes and further observe the reaction of the tissues to various environmental factors or drug factors.
At present, a plurality of methods for mouse heart perfusion are introduced, but the method is not suitable for experimental teaching due to complex operation and high cost. When experimental teaching, insert mouse left ventricle with the syringe needle, cut off mouse atrium simultaneously to make blood flow, take out the syringe needle after the perfusion, and pour the blood in the tray to in the waste liquid bucket. In this operation process, on the one hand, blood is difficult for discharging totally, can cause the influence to the experimental determination of tissue itself, and on the other hand blood pollutes the laboratory bench easily.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a blood that not only can make the effectual replacement of physiological solution experimental part reduces the influence of blood to the experimental determination of tissue itself, can also collect blood in the replacement, avoids polluting a mouse heart perfusion device of laboratory bench.
In order to solve the technical problem, the utility model discloses the technical scheme who takes is:
a mouse heart perfusion device, comprising:
the injection extractor comprises an injection extraction cylinder made of transparent materials, a push-pull rod arranged in the injection extraction cylinder and a sealing plug fixedly arranged at the rear end in the injection extraction cylinder, wherein a piston is fixed at the front end of the push-pull rod, the rear end of the push-pull rod penetrates through the sealing plug and extends out of the injection extraction cylinder, and the push-pull rod moves axially along the injection extraction cylinder along with the piston, so that an inner cavity of the injection extraction cylinder forms an independent blood storage cavity and a physiological solution cavity at the front end and the rear end of the piston, a blood extraction nozzle is arranged on the end face of the front end of the blood storage cavity, and an injection nozzle is arranged on the side wall of the rear end of;
the blood drawing assembly comprises a first hose, a first connecting head, a needle head protecting sleeve, a fixed sucker and a bleeding needle, wherein the first connecting head is arranged at one end of the first hose and is in threaded connection with a blood drawing nozzle, the other end of the first hose is connected with one end of the needle head protecting sleeve, the other end of the needle head protecting sleeve is fixed with the fixed sucker made of rubber, the bleeding needle is fixed in the needle head protecting sleeve, and the sharp end of the bleeding needle extends into the fixed sucker; and
the injection assembly comprises a second hose, a second connector and an injection needle, wherein the second connector is arranged at one end of the second hose and is in threaded connection with the injection nozzle, and the other end of the second hose is connected with the injection needle.
The injection needle is connected with a second hose by means of a fixing piece, a conical flow passage is arranged on the fixing piece, the injection needle is inserted into the small end of the flow passage, and the second hose is fixed with the large end of the flow passage.
A further technical scheme is that the front end of the fixing piece is protruded with a barb structure arranged along the direction of the injection needle.
The technical scheme is that the sealing plug is made of rubber, a circle of positioning protrusion is arranged on an injection extraction cylinder at the front end of the sealing plug, a positioning cap in threaded connection with the outer wall of the injection extraction cylinder is arranged at the rear end of the injection extraction cylinder, and the positioning cap is screwed inwards to extrude the sealing plug.
The technical scheme is that the sealing plug comprises a positioning plug made of hard materials, the positioning plug is in threaded connection with the inner wall of the rear end of the injection extraction cylinder, and a plurality of sealing rings are arranged on the positioning plug at intervals in the through hole for penetrating the push-pull rod.
The further technical proposal is that the injection needle is a No. 4, No. 4.5, No. 5 or No. 5.5 needle.
A section of corrugated pipe made of rubber is arranged between the needle head protecting sleeve and the fixed sucker.
Adopt the produced beneficial effect of above-mentioned technical scheme to lie in:
the perfusion device integrates injection propulsion and blood extraction, realizes the injection of physiological solution and the extraction and storage of blood by utilizing the movement of a piston and the pressure variation, and establishes a physiological solution replacement closed loop through a body circulation system of a heart. The push-pull rod is pulled to compress the physiological solution cavity to generate thrust for injecting the physiological solution into the left ventricle, and meanwhile, the blood storage cavity is expanded to generate suction for drawing blood from the right atrium, so that the blood is replaced by the physiological solution, the influence of the blood on the experimental determination of the tissue can be reduced, and the reaction of the tested tissue to environmental factors or drug factors can be reflected more truly.
And the perfusion device collects the flowing blood while perfusing physiological solution into the body for circulation, thereby avoiding the pollution of the blood to the experiment table and being convenient to operate.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a schematic view of the injection extractor of the present invention;
fig. 3 is a schematic structural view of the blood drawing assembly of the present invention;
fig. 4 is a schematic view of the installation structure of the sealing plug in the first embodiment of the present invention;
fig. 5 is a schematic structural diagram of a sealing plug in the second embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, not all, embodiments of the present invention. Based on the embodiments only in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be implemented in other ways different from the specific details set forth herein, and one skilled in the art may similarly generalize the present invention without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
As shown in fig. 1 to 3, a mouse heart perfusion device comprises an injection extractor, a blood drawing assembly and an injection assembly. The mice are killed or anesthetized by the spinal cord cutting method, the chest cavities of the mice are opened, constant-temperature physiological saline solution (or wilsonii fluid) is perfused into the left ventricle of the mice through the injection assembly, and blood flowing out of the right atrium of the mice is recovered through the blood drawing assembly to form a physiological saline solution (or wilsonii fluid) replacement closed loop.
The injection extractor comprises an injection extraction cylinder 11 made of transparent materials, a push-pull rod 12 arranged in the injection extraction cylinder 11 and a sealing plug 13 fixedly arranged at the rear end in the injection extraction cylinder 11, wherein the sealing plug 13 can prevent liquid in the injection extraction cylinder 11 from leaking outwards, a piston 14 is fixedly arranged at the front end of the push-pull rod 12 to form a piston push rod in the existing injector, the rear end of the push-pull rod 12 penetrates through the sealing plug 13 and extends out of the injection extraction cylinder 11, the push-pull rod 12 moves axially along the injection extraction cylinder 11, so that a blood storage cavity 01 and a physiological solution cavity 02 are formed in the front end and the rear end of the piston 14 in the inner cavity of the injection extraction cylinder 11, a sealing ring is arranged on the piston 14, the blood storage cavity 01 and the physiological solution cavity 02 exist independently through the arrangement of the piston 14, the two cavities are sealed and not communicated, and a blood extraction nozzle 111 is arranged on the end face at the, an injection nozzle 112 is arranged on the side wall of the back end of the physiological solution cavity 02.
The blood drawing component comprises a first hose 21, a first connecting head 22, a needle head protecting sleeve 23, a fixed suction cup 24 and a blood drawing needle 25, wherein the first connecting head 22 is arranged at one end of the first hose 21 and is in threaded connection with a blood drawing nozzle 111, the other end of the first hose 21 is connected with one end of the needle head protecting sleeve 23, the other end of the needle head protecting sleeve 23 is fixed with the fixed suction cup 24 made of rubber materials, the blood drawing needle 25 is fixed in the needle head protecting sleeve 23, and the sharp end of the blood drawing needle extends into the fixed suction cup 24. The material of the fixed suction cup 24 is soft rubber with low sulfur content and good elasticity and flexibility, and can deform along with the pressure change.
The injection assembly comprises a second hose 31, a second connector 32 and an injection needle 33, wherein the second connector 32 is arranged at one end of the second hose 31 and is in threaded connection with the injection nozzle 112, and the other end of the second hose 31 is connected with the injection needle 33.
When the perfusion device is used:
firstly, filling physiological solution into a physiological solution cavity 02 of an injection extractor, pulling a piston 14 to one end of a sealing plug 13 to completely compress the physiological solution cavity 02, then connecting an injection nozzle 112 with a physiological solution bottle, pushing a push-pull rod 12 inwards to suck the physiological solution into the physiological solution cavity 02 until the volume of the physiological solution cavity 02 is enlarged to the inner cavity of the whole injection extractor 11, and then completely compressing a blood storage cavity 01;
secondly, assembling, namely connecting the blood drawing assembly with a blood drawing nozzle 111 of the injection extractor through the first connector 22, and connecting the injection assembly with an injection nozzle 112 of the injection extractor through the second connector 32;
thirdly, the fixed suction cup 24 of the blood drawing component is stuck to the right atrium wall of the mouse, and the injection needle 33 of the injection component is penetrated into the left ventricle of the mouse;
fourthly, the push rod 12 is pulled outwards, the physiological solution cavity 02 is gradually compressed and reduced, the physiology inside the physiological solution cavity is easily injected into the left ventricle of the mouse through the injection assembly, meanwhile, the volume of the blood storage cavity 01 is gradually enlarged, the internal air pressure is reduced, the fixed sucker 24 deforms to enable the bloodletting 25 to gradually contact the wall of the right atrium until the bloodletting is punctured, the pressure inside the blood storage cavity 01 is always lower than the outside along with the pulling of the piston 14, the blood continuously enters the blood storage cavity 01 through the bloodletting assembly until the blood storage cavity 01 is enlarged to the inner cavity of the whole injection extraction cylinder 11, at the moment, the physiological solution cavity 02 is completely compressed, and the blood with the fixed dilution factor is stored inside the blood storage cavity 01.
The perfusion device integrates injection propulsion and blood extraction, realizes the injection of physiological solution and the extraction and storage of blood by utilizing the movement of a piston and the pressure variation, and establishes a physiological solution replacement closed loop through a body circulation system of a heart. The push-pull rod 12 is pulled to compress the physiological solution cavity 02 to generate a pushing force for injecting the physiological solution into the left ventricle, and meanwhile, the blood storage cavity 01 is expanded to generate a suction force for drawing blood from the right atrium, so that the blood is replaced by the physiological solution, the influence of the blood on the experimental determination of the tissue can be reduced, and the reaction of the tested tissue to environmental factors or drug factors can be reflected more truly.
In addition, the perfusion device collects the flowing blood while perfusing physiological solution into the body for circulation, and recovers the whole blood, thereby avoiding the pollution of the blood to the experiment table and being convenient to operate.
Since the diameter of the injection needle 33 is different from the diameter of the second tube 31, and the injection needle 33 cannot be directly connected to the second tube 31, the injection needle 33 is connected to the second tube 31 by a fixing piece 34, the fixing piece 34 is provided with a tapered flow passage, the injection needle 33 is inserted into the small end of the flow passage, and the second tube 31 is fixed to the large end of the flow passage. In addition, the fixing plate 34 can be conveniently held by hand to control the injection assembly to accurately penetrate into the corresponding part, and the injection assembly can be fixed by hand during perfusion to prevent the injection assembly from being separated from the heart.
Further, the front end protrusion of stationary blade 34 has the barb structure that sets up along the syringe needle 33 direction, and the barb structure can pierce mouse left ventricle along with syringe needle 33, and after barb part got into the heart wall, because the deformation of heart wall tissue resumes, can increase the degree of difficulty that syringe needle 33 pulled out, prevents that syringe needle 33 from breaking away from, improves the steadiness of needle tubing.
Two embodiments are listed with respect to the form of mounting of the sealing plug 13.
Example one
As shown in fig. 4, the sealing plug 13 is made of rubber, a circle of positioning protrusions 15 is arranged on the injection extraction cylinder 11 at the front end of the sealing plug 13, a positioning cap 16 in threaded connection with the outer wall of the injection extraction cylinder 11 is arranged at the rear end of the injection extraction cylinder 11, and the positioning cap 16 is screwed inwards to extrude the sealing plug 13.
The sealing plug 13 is axially positioned through the positioning protrusion 15 and the positioning cap 16, and the positioning cap 16 is screwed inwards to press the sealing plug 13 inwards, so that the sealing plug 13 is compressed and deformed, on one hand, the inner hole of the sealing plug is reduced, and the sealing between the sealing plug 13 and the push-pull rod 12 is enhanced. On the other hand, the outer ring of the sealing plug 13 is expanded, and the sealing between the sealing plug 13 and the inner wall of the injection and extraction cylinder 11 is enhanced.
Example two
As shown in fig. 5, the sealing plug 13 includes a positioning plug 131 made of a hard material (such as plastic, metal, etc.), the positioning plug 131 is in threaded connection with the inner wall of the rear end of the injection extraction cylinder 11 to fix the sealing plug 13 and the injection extraction cylinder 11, a plurality of sealing rings 132 are arranged at intervals in a through hole for penetrating the push-pull rod 12 on the positioning plug 131 to seal the sealing plug 13 and the push-pull rod 12, so that the leakage of the physiological solution is avoided, and simultaneously, the physiological solution can be filled into the physiological solution cavity 02 and discharged from the injection nozzle 112.
The injection needle 33 is a 4-gauge, 4.5-gauge, 5-gauge or 5.5-gauge needle, and the outer diameter of the needle is preferably about 0.5 mm.
Still be equipped with the bellows 26 of one section rubber material between syringe needle lag 23 and fixed suction cup 24, can compress the distance between syringe needle lag 23 and the fixed suction cup 24 through setting up of bellows 26, make bloodletting needle 25 protrusion fixed suction cup 24 when using, pierce mouse right ventricle, when not using, in bloodletting needle 25 can retract fixed suction cup 24, prevent to cut the people.
The above is only the preferred embodiment of the present invention, and any person can make some simple modifications, deformations and equivalent replacements according to the present invention, all fall into the protection scope of the present invention.
Claims (7)
1. A mouse heart perfusion device, comprising:
the injection extractor comprises an injection extraction cylinder (11) made of transparent materials, a push-pull rod (12) arranged in the injection extraction cylinder (11) and a sealing plug (13) fixedly arranged at the rear end in the injection extraction cylinder (11), wherein a piston (14) is fixed at the front end of the push-pull rod (12), the rear end of the piston (14) penetrates through the sealing plug (13) and extends out of the injection extraction cylinder (11), the push-pull rod (12) moves axially along the injection extraction cylinder (11), so that an independent blood storage cavity (01) and a physiological solution cavity (02) are formed in the front end and the rear end of the piston (14) in the inner cavity of the injection extraction cylinder (11), a blood extraction nozzle (111) is arranged on the end face of the front end of the blood storage cavity (01), and an injection nozzle (112) is arranged on the side wall of the rear end of the physiological solution cavity (;
the blood drawing assembly comprises a first hose (21), a first connecting head (22), a needle head protecting sleeve (23), a fixed sucker (24) and a bleeding needle (25), wherein the first connecting head (22) is arranged at one end of the first hose (21) and is in threaded connection with a blood drawing nozzle (111), the other end of the first hose (21) is connected with one end of the needle head protecting sleeve (23), the other end of the needle head protecting sleeve (23) is fixed with the fixed sucker (24) made of rubber, the bleeding needle (25) is fixed in the needle head protecting sleeve (23), and the sharp end of the bleeding needle extends into the fixed sucker (24); and
injection assembly, including second hose (31), second connector (32) and syringe needle (33), the one end of second hose (31) and with injection nozzle (112) threaded connection are located in second connector (32), the other end and syringe needle (33) of second hose (31) are connected.
2. A perfusion device for the heart of a mouse as claimed in claim 1, wherein the injection needle (33) is connected to the second tube (31) by a fixing piece (34), the fixing piece (34) is provided with a tapered flow passage, the injection needle (33) is inserted into the small end of the flow passage, and the second tube (31) is fixed to the large end of the flow passage.
3. A perfusion device for the heart of a mouse, as claimed in claim 2, wherein the fixing plate (34) has a barb projecting from the front end thereof in the direction of the injection needle (33).
4. A perfusion device for the heart of a mouse according to claim 1, wherein the sealing plug (13) is made of rubber, a circle of positioning protrusions (15) are arranged on the injection and extraction tube (11) at the front end of the sealing plug (13), a positioning cap (16) in threaded connection with the outer wall of the injection and extraction tube (11) is arranged at the rear end of the injection and extraction tube (11), and the positioning cap (16) is screwed inwards to press the sealing plug (13).
5. The mouse heart perfusion device according to claim 1, wherein the sealing plug (13) comprises a positioning plug (131) made of a rigid material, the positioning plug (131) is in threaded connection with an inner wall of the rear end of the injection and extraction cylinder (11), and a plurality of sealing rings (132) are arranged on the positioning plug (131) at intervals in a through hole for penetrating the push-pull rod (12).
6. A mouse heart perfusion device according to claim 1, wherein the injection needle (33) is a 4-gauge, 4.5-gauge, 5-gauge or 5.5-gauge needle.
7. A perfusion device according to claim 1, wherein a rubber bellows (26) is further provided between the needle shield (23) and the fixed suction cup (24).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821378338.0U CN211460665U (en) | 2018-08-24 | 2018-08-24 | Mouse heart perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821378338.0U CN211460665U (en) | 2018-08-24 | 2018-08-24 | Mouse heart perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211460665U true CN211460665U (en) | 2020-09-11 |
Family
ID=72368525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821378338.0U Expired - Fee Related CN211460665U (en) | 2018-08-24 | 2018-08-24 | Mouse heart perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211460665U (en) |
-
2018
- 2018-08-24 CN CN201821378338.0U patent/CN211460665U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200911 |