CN211430515U - A hydrogen inhalation device and its system for studying the inhalation of hydrogen by animals - Google Patents

Abstract

The utility model relates to the technical field of animal experiments, in particular to a hydrogen absorption device and a system thereof for studying the inhalation of hydrogen by animals. Diffusion area and animal activity area, a gas inlet and a gas outlet are arranged on the wall of the gas diffusion area, an air inlet duct connected to the gas inlet is arranged in the gas diffusion area, and an air inlet duct is arranged on the air inlet duct. There are multiple gas diffusion ports to ensure even gas escape within the animal box. The animal box of the utility model separates the animal activity area and the gas diffusion area, and the gas diffusion ports are evenly distributed in the gas diffusion area, which is conducive to maintaining the uniform and stable gas environment in the animal box and ensures that the animals inhale evenly mixed hydrogen.

Description

A hydrogen inhalation device and its system for studying the inhalation of hydrogen by animals

technical field

The utility model belongs to the technical field of animal experiments, in particular to a hydrogen absorption device used for studying the inhalation of different concentrations of hydrogen by animals.

Background technique

Molecular hydrogen has long been considered a physiologically inert gas. Although an article published in the internationally renowned journal "Science" in 1975 reported the use of the antioxidant effect of high-pressure hydrogen to treat squamous cell carcinoma of the skin, due to the high concentration of hydrogen, various detection techniques and protective measures were limited at that time, and the operation The difficulty is high, and the article has not attracted the attention of the scientific community. Until 2007, Japanese professor Ota Chengao and others published an article in "Nature Medicine", reporting that animals breathing 2% hydrogen can effectively scavenge free radicals in the body, significantly improve cerebral ischemia-reperfusion injury, and it plays a role. The basis is the selective scavenging of free radicals by hydrogen molecules, that is, mainly scavenging harmful hydroxyl radicals and nitrite anions. The report completely changed the understanding of hydrogen in academia, and quickly attracted widespread attention from scholars in Japan, the United States, and China. Subsequently, the research on the biomedical effects of hydrogen molecules has blossomed and has been effectively verified in various disease models and clinical trials. And with the development of related industries, hydrogen supply methods tend to be diversified. So far, the main methods of using hydrogen to treat diseases include breathing hydrogen, drinking hydrogen-rich water, injecting hydrogen-rich saline, and bathing with hydrogen water. The methods used for animal experiments are mainly concentrated in the first three. Among them, the method of drinking hydrogen-rich water is not easy to control. There is a big difference in the drinking amount of each animal, and the hydrogen content will be greatly reduced with the drinking time. Without affecting the normal diet and drinking water of animals, the actual intake is not easy. control. The method of intraperitoneal injection of hydrogen-rich normal saline will affect the amount of hydrogen intake due to the limitation of the injection volume of animals, and the operation is relatively troublesome. Giving hydrogen by breathing can not only make the gas enter the body quickly, but also control the intake of different concentrations of hydrogen by adjusting the percentage of hydrogen in the mixture. In recent years, there have been devices that provide hydrogen through electrolysis of water. However, the hydrogen concentration of electrolyzed water is 66.7%, and the oxygen concentration is 33.3%. The proportion of oxygen content does not meet the normal oxygen content in the atmosphere (21%). It is necessary to give hydrogen in animal experiments.

Utility model content

The utility model provides a hydrogen inhalation device for studying the inhalation of hydrogen of different concentrations by animals, which can accurately provide the oxygen required for the animals to breathe and hydrogen of a specific concentration, so as to accurately study the influence of different concentrations of hydrogen on the indicators to be measured in experimental animal models Provide the basis.

The hydrogen absorption device of the utility model comprises a closed animal box, the animal box is divided into a gas diffusion area and an animal activity area by a partition plate with holes, and a gas inlet and a gas outlet are arranged on the wall of the gas diffusion area, The gas diffusion area is provided with an air intake conduit connected to the gas inlet, and a plurality of gas diffusion ports are arranged on the air intake conduit to ensure that the gas escapes evenly in the animal box.

By setting the animal box into a gas diffusion area and an animal activity area, the hydrogen absorption device of the utility model is beneficial to arranging an air intake duct with a gas diffusion port in the gas diffusion area, so as to realize the control of the gas diffusion, which is beneficial to the change of the gas. For a uniform distribution, improve the accuracy of the experiment.

Preferably, the perforated partition is arranged in the middle and lower part of the animal box, and divides the animal box into a lower gas diffusion area and an upper animal activity area. When the proportion of hydrogen in the intake air increases, the density of the gas is lower than that of the air, the gas diffuses from bottom to top, and is evenly distributed in the animal activity area, which is conducive to the effective inhalation of hydrogen by the animals. After the upper part of the gas is used by the animal, it is discharged from the lower part of the gas outlet under the action of pressure, which is beneficial to the animal to make full use of the gas and form a stable gas environment in the animal activity area.

Preferably, a fan is provided in the gas diffusion zone to further fully mix the gas led out of the air intake duct.

Preferably, an absorption device for absorbing carbon dioxide and water produced by the animal's breathing is also provided in the animal box. By setting the above device, the carbon dioxide and water generated during the animal's respiration can be adsorbed in time to keep the gas environment stable.

Preferably, the absorption device is arranged at the bottom of the gas diffusion zone.

As a preferred operation mode, the absorbing material in the absorbing device is a mixture of soda lime and discolored silica gel, and the absorbing material is spread on the bottom of the gas diffusion zone. The density of carbon dioxide and water vapor is relatively high, and placing the absorbing material in the controlled atmosphere area (bottom) is conducive to fully absorbing carbon dioxide and water, and maintaining a constant gas concentration in the animal box.

Preferably, the ratio of the diameter of the gas inlet to the gas outlet is 1:1-2. The diameter of the gas outlet is larger than the diameter of the inlet, which can ensure the safe operation of the device and prevent the pressure in the device from being too large.

Preferably, the gas outlet is connected to an air outlet pipe, and the air outlet pipe leads to the outdoor atmosphere. Through the above settings, the safety of the environment in the laboratory can be guaranteed.

As a preferred solution, the hydrogen absorption device includes a closed animal box, the animal box is divided into a lower gas diffusion area and an upper animal activity area by a perforated partition, and the wall of the gas diffusion area is provided with gas The inlet and the gas outlet, the ratio of the diameter of the gas inlet to the gas outlet is 1:1 to 2, and a gas inlet duct located in the gas diffusion area and connected to the gas inlet is also provided, and the gas inlet duct is provided with There are a plurality of gas diffusion ports, the gas diffusion ports are evenly distributed in the gas diffusion area, and a fan is arranged in the gas diffusion area to further fully mix the gas derived from the gas conduit, and the gas diffusion area is provided with a fan. An absorption device for absorbing carbon dioxide and water produced by animal respiration is arranged inside.

Preferably, the outer casing of the animal box is provided with an explosion-proof box. Since the hydrogen absorption device of the present invention involves the use of hydrogen in the process of use, in order to further ensure safety, an explosion-proof box is arranged on the outer casing of the animal box.

The utility model also protects the air-conditioning system including the hydrogen absorption device of the utility model, including a gas storage tank, a rotameter and a gas mixing device, and the gas storage tank is connected to the rotameter and the gas mixing device in sequence, The gas mixing device is connected to the gas inlet of the animal box.

By arranging the gas mixing device in the system, the utility model is favorable for the gas to be fully mixed.

Preferably, the gas storage tanks are hydrogen cylinders, oxygen cylinders and nitrogen cylinders.

Preferably, a gas pressure reducing valve is further provided between the gas storage tank and the rotameter.

Preferably, the air conditioning system of the present invention further comprises an alarm gas cylinder cabinet, and the gas storage tank is arranged in the alarm cylinder cabinet.

The utility model has the following beneficial effects:

1) The device of the present utility model can ensure the uniform distribution of gas in the device by setting a gas diffusion zone and improve the corresponding structure of the zone, providing a stable hydrogen environment for the growth of experimental animals, and the device has a simple structure, which is conducive to to be used on a large scale.

2) The hydrogen absorption system described in the present utility model meets the requirements of different concentrations of hydrogen in the animal box by setting a rotameter, and ensures that the oxygen concentration in the box is 21%, which is different from that in atmospheric conditions. Regular oxygen levels remain the same.

Description of drawings

Fig. 1 is the structural representation of the animal box described in Example 1;

2 is a schematic structural diagram of the air conditioning system described in Embodiment 2;

In the figure: 1 is explosion-proof box; 2 is animal box; 3 is animal activity area; 4 is partition plate with holes; 5 is gas diffusion area; 6 is intake duct; 7 is absorption device; 8 is gas inlet; 9 is 10 is a gas outlet; 11 is a pressure reducing valve; 12 is an alarm gas cylinder cabinet; 13 is an oxygen cylinder; 14 is a nitrogen cylinder; 15 is a hydrogen cylinder; 16 is a rotameter; 17 is a gas mixing device.

Detailed ways

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

Example 1

This embodiment relates to a hydrogen inhalation device for animals to inhale hydrogen (the schematic diagram of the structure is shown in Figure 1). The box is divided into a lower gas diffusion area 5 and an upper animal activity area 3 by a perforated partition 4, and a gas inlet 8 and a gas outlet 10 are provided on the wall of the gas diffusion area. The gas inlet 8 and the gas outlet 10 The ratio of the diameters of the air inlets is 1:2, and there is also an air inlet duct 6 located in the gas diffusion area and connected to the gas inlet. The air inlet duct is provided with a plurality of gas diffusion ports, and the gas diffusion ports Evenly distributed in the gas diffusion zone, a fan 9 is arranged in the gas diffusion zone to further fully mix the gas derived from the gas conduit, and carbon dioxide and carbon dioxide produced by absorbing animal breathing are arranged in the gas diffusion zone. Water absorption device 7, the absorption materials in the absorption device are soda lime and discolored silica gel, which are evenly laid on the bottom of the gas diffusion zone.

Example 2

This embodiment relates to an air conditioning system for animals to inhale hydrogen (the schematic diagram of its structure is shown in Figure 2), including gas storage tanks: a hydrogen cylinder 15, an oxygen cylinder 13, a nitrogen cylinder 14, a rotameter 16, and the gas storage tank There is also a gas pressure reducing valve 11, a gas mixing device 17 and an alarm gas cylinder cabinet 12 between the rotameter and the rotameter; the gas storage tank is arranged in the alarm cylinder cabinet 12, and is connected with the rotameter 16. It is connected to the gas mixing device 17 in sequence. The gas mixing device 17 is connected to the gas inlet of the animal box 2 described in Embodiment 1, and the gas outlet of the animal box 2 is connected to the outdoor atmosphere through a pipeline.

Example 3

The present embodiment relates to a method for using the modified atmosphere device described in Embodiment 2 to provide hydrogen with different concentrations to animals, including the following steps:

1) put 6 rats with a body weight of 300～400g into the animal box of Example 1, and the size of the animal box is 60cm×40cm×30cm (length×width×height);

2) Adjust the gas storage tank and the rotameter so that the proportion of hydrogen is the required concentration (such as 2% for low concentration, or 67% for high concentration), the proportion of oxygen is 21%, and the remainder is nitrogen, at a speed of 3L/min Into the animal box, the rat inhaled hydrogen for 2 hours, and the hydrogen and oxygen detectors were used to detect the change of gas concentration in the animal box in real time during the inhalation process. It was found that the concentration of hydrogen in the incubator was constant at The desired concentration, the concentration of oxygen is constant at 21% ± 1%.

Although, the present utility model has been described in detail above with general description, specific embodiments and experiments, but on the basis of the present utility model, some modifications or improvements can be made to it, which is very important for those skilled in the art. Obvious. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention belong to the scope of protection of the present invention.

Claims (10)

1. a hydrogen-absorbing device for researching animal inhalation hydrogen, is characterized in that, described hydrogen-absorbing device comprises an airtight animal box, and described animal box is divided into gas diffusion zone and animal activity zone by perforated clapboard, so The wall of the gas diffusion zone is provided with a gas inlet and a gas outlet, the gas diffusion zone is provided with an intake conduit connected to the gas inlet, and the intake conduit is provided with a plurality of gas diffusion ports to ensure The gas escapes uniformly within the animal box. 2 . The hydrogen absorption device according to claim 1 , wherein the perforated partition plate is arranged in the middle and lower part of the animal box, and the animal box is divided into a gas diffusion zone in the lower part and an animal activity in the upper part. 3 . Area. 3 . The hydrogen absorption device according to claim 2 , wherein a fan is provided in the gas diffusion area to further fully mix the gas derived from the intake duct. 4 . 4 . The hydrogen absorption device according to claim 3 , wherein the animal box is further provided with an absorption device for absorbing carbon dioxide and water produced by animal breathing. 5 . 5 . The hydrogen absorption device according to claim 4 , wherein the absorption device is placed at the bottom of the gas diffusion zone. 6 . 6 . The hydrogen absorption device according to claim 1 , wherein the ratio of the diameter of the gas inlet to the gas outlet is 1:1-2. 7 . 7 . The hydrogen absorption device according to claim 6 , wherein the gas outlet is connected to an air outlet duct, and the air outlet duct leads to the outdoor atmosphere. 8 . 8. The hydrogen absorption device according to claim 1 or 2, characterized in that, an explosion-proof box is provided on the outer casing of the animal box. 9. The air conditioning system comprising the hydrogen absorption device according to any one of claims 1 to 8, further comprising a gas storage tank, a rotameter and a gas mixing device, the gas storage tank and the rotameter It is connected with the gas mixing device in sequence, and the gas mixing device is connected with the gas inlet of the animal box. 10 . The air conditioning system according to claim 9 , wherein the gas storage tank is a hydrogen cylinder, an oxygen cylinder and a nitrogen cylinder, and a gas reducer is also provided between the gas storage tank and the rotameter. 11 . pressure valve.

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