CN210995660U - A separation washing unit for experimental animals intestinal - Google Patents

Abstract

The utility model discloses an in vitro washing unit for experimental animals intestinal, include: one end of the connecting pipe (1) extends into the intestinal tract of the animal, and the other end is connected with the flushing pipe; one end of the flushing pipe (2) is connected with the connecting pipe, and the other end of the flushing pipe is connected with the liquid feeding device; the intestinal canal fixer (3) comprises a first clamping piece (31) and a second clamping piece (32) for clamping the connecting pipe and the intestinal canal of the animal, and an adjuster (33) for adjusting the distance between the first clamping piece and the second clamping piece; the iron support (4) is used for fixing the intestinal fixer; a liquid receiver (5) is positioned below the intestinal canal fixer. The flushing device provided by the utility model has simple structure and convenient use, can complete the automatic flushing of intestinal tracts, and improves the progress of the whole experiment; in addition, the connecting pipe adopts conical setting, is convenient for penetrate and take out in the intestinal, and the flushing pipe can connect the dropping funnel and utilize the difference in height to wash the intestinal, also can select to set up peristaltic pump flow rate and wash.

Description

An in vitro irrigation device for the intestinal tract of experimental animals

technical field

The utility model relates to the field of laboratory equipment, in particular to an isolated flushing device for the intestinal tract of experimental animals.

Background technique

The intestinal tract is not only the most important "absorption" and "excretion" channel of the animal body, but also the largest micro-ecosystem. The ecological balance of the intestinal flora is an important factor in determining the health of the body. The normal flora structure can promote the health of the body, while the imbalance of the flora can lead to the occurrence of various diseases and sometimes systemic disorders. Studies have shown that the gut microbiota is associated with appetite control, energy balance, obesity, diabetes, immune function, allergies, behavioral disturbances, cardiovascular disease, and cancer. Effective irrigation of the lumen of intestinal tissues of different intestinal segments (such as duodenum, empty field, ileum, cecum, colon, and rectum) and the acquisition of the contents of different intestinal segments are important in the study of intestinal and intestinal flora. very important work.

The laboratory mostly uses small experimental animals such as rats, mice and rabbits to carry out research on the intestinal and intestinal flora. The acquisition of intestinal contents and the washing of intestinal tissues are an important part of sample collection in the anatomy of experimental animals. .

The current routine intestinal cleaning methods are as follows: (1) After removing the target intestinal segment, soak it in a vessel containing buffer solution, squeeze or fiddle with the outside of the intestinal wall with tweezers, take out the intestinal tissue and rinse it again in clean buffer solution After washing, a large amount of buffer solution containing intestinal contents in the vessel was transferred to a centrifuge tube; (2) the experimenter used forceps to hold the intestinal tract in one hand, and the other hand held a syringe and inserted it into the intestinal tract to flush the intestinal tract. The above two conventional methods mainly have the following disadvantages: (1) The intestines (especially the small intestines) of small experimental animals are usually fragile, and when the experimenter squeezes, fiddles or flushes the intestinal tissue, it is often easy to damage the intestinal tissue (intestinal wall and intestinal tract). (2) The intestinal (especially the small intestine) of small experimental animals is usually soft and thin, and it requires the experimenter to have a fine and Stable control ability, unable to liberate the hands of the experimenter; (3) unable to control the flow rate of flushing, which may cause damage to the integrity of the intestinal tissue inside and outside, affecting the follow-up observation of intestinal structure and other research; (4) unable to Controlling the amount of washing buffer used may cause the intestinal contents to be randomly diluted in different proportions, which will affect the subsequent qualitative and quantitative analysis of the intestinal microbial or biochemical composition in the intestinal contents; (4) Washing time It is difficult to control, and the flushing efficiency is low, which affects the progress of the entire experiment. However, there is currently no simple and efficient device for in vitro intestinal irrigation of experimental animals.

SUMMARY OF THE INVENTION

In view of the deficiencies of the prior art, the present utility model provides an in vitro rinsing device for the intestinal tract of experimental animals, which can automatically rinse the intestinal tract, accurately control the flow rate and the amount of rinsing buffer used, and can avoid the intestinal tract that may be caused by manual operation. Mechanical damage to the tissue, reduce the workload of the experimenter, and improve the efficiency of the experiment.

In order to solve the above-mentioned technical problems, the technical solution of the present invention is as follows: an in vitro rinsing device for the intestinal tract of experimental animals, the rinsing device comprises:

a connecting pipe, one end of the connecting pipe extends into the animal intestine, and the other end is connected with the flushing pipe; the connecting pipe is conical, with a height of 2-5 cm and a bottom diameter of 0.2-0.8 cm;

a flushing pipe, one end of the flushing pipe is connected with the connecting pipe, and the other end is connected with the liquid feeder;

an intestinal fixer, the intestinal fixer includes a first clip and a second clip for clamping the connecting tube and the animal intestine, and a regulator for adjusting the distance between the first clip and the second clip;

a liquid receiving device, the liquid receiving device is located below the intestinal fixer;

An iron stand, on which a liquid receiver is placed, and a bracket of the iron stand is used to fix the intestinal fixer.

Further, the liquid feeder is one or more of a peristaltic pump, a syringe and a dropping funnel.

Further, the flushing pipe is an in-line flushing pipe or a Y-shaped flushing pipe. You can choose a suitable flushing tube according to your needs. When you choose a Y-shaped flushing tube, one side of the top can be connected to a peristaltic pump (the water inlet of the peristaltic pump is connected to a centrifuge tube with a precise scale to control the amount of buffer solution), and the other end is connected to a syringe or drip. There is a flow controller on the funnel and the flushing pipe, so the appropriate flushing structure can be selected according to the situation.

Further, the first clip and the second clip are provided with arc-shaped grooves at positions in contact with the intestinal tract. The curved groove is used to increase the contact area with the intestine and avoid slippage.

Further, the flushing pipe or the liquid feeder is fixed on the iron stand.

Further, the regulator is a knob screw, the first clip is arranged on the first fixing plate, the knob screw is arranged on the second fixing plate, the end of the knob screw is connected with a second clip, the first clip is Opposite to the second clip. The first fixing plate and the second fixing plate are both arranged on the fixing seat, the fixing seat is fixed on the iron frame, and the fixing seat is also connected with a third fixing plate and a fixing rod for fixing the liquid feeder and/or the flushing pipe .

Optionally, the adjuster is an adjusting bolt and an adjusting nut, the ends of the first clip and the second clip are connected by a rotating shaft, a spring and an adjusting bolt are arranged between the two, and the outside of the first clip or the second clip is An adjusting nut is provided; the end of the first clip or the second clip is provided with a handle for fixing on the iron stand.

Optionally, the adjuster is a spring clip, the first clip and the second clip are hinged together through the spring clip, and the distance between the first clip and/or the second clip is adjusted by pressing the tail end of the first clip and/or the second clip, The end of the first clip or the second clip is provided with a handle for fixing on the iron stand.

The working principle of the utility model is as follows: because the intestine is relatively soft, it is difficult for the experimenter to extend the flushing tube into the intestine when flushing, and manual flushing takes up time and affects the experimental efficiency. In view of the above problems, the present utility model provides a A device that automatically flushes the intestines. After taking out a section of the intestines of rats, mice and other animals, a conical connecting tube is inserted into one end of the intestines to support the intestines to facilitate the entry of the solution. The channel holder is fixed, the upper end of the connecting pipe is connected to the flushing pipe, and the flushing pipe is connected to the dropping funnel or the peristaltic pump to complete the automatic flushing.

Compared with the prior art, the present utility model has the following beneficial effects: the flushing device provided by the present utility model has a simple structure, is convenient to use, can complete the automatic flushing of the intestinal tract, accelerates the whole experiment progress, and does not cause mechanical damage to the intestinal tract. In addition, the connecting tube adopts a conical configuration, which is convenient for penetration and removal in the intestinal tract, and can also support the intestinal tract for easy fixation; the irrigation tube can be connected to a drip funnel to flush the intestinal tract with the height difference, or a peristaltic pump can be selected to control the flow rate or Flow flushing allows precise control of flushing time, flow rate and flow to avoid damage to the integrity of the bowel.

Description of drawings

1 is a schematic structural diagram of the flushing device provided in Example 1 (the connecting tube is not inserted into the animal intestine in the figure);

Fig. 2 is the connection relation diagram of clip, connecting tube and animal intestine;

3 is a schematic structural diagram of the flushing device provided in Example 2;

Figure 4 is a schematic structural diagram of an intestinal fixer;

Marked in the figure: 1-connecting tube, 2-irrigation tube, 3-intestinal fixator, 4-iron stand, 5-liquid receiver, 6-peristaltic pump, 7-syringe, 8-dropping funnel, 9-arc Shape groove, 10- animal intestine, 11- flow controller, 12- adjusting bolt, 13- adjusting nut, 31- first clip, 32- second clip, 33- knob screw, 34- first fixing plate , 35-Second fixed plate, 36-Fixed seat, 37-Third fixed plate, 38-Fixed rod.

Detailed ways

The technical solutions of the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments, but the present invention is not limited to the following technical solutions.

Example 1

As shown in Figure 1 and Figure 2, the flushing device includes:

A connecting pipe 1, one end of the connecting pipe 1 extends into the animal intestine 10, and the other end is connected with the flushing pipe 2; the connecting pipe 1 is conical, with a height of 3-4 cm, and a bottom diameter of 0.2-0.5 cm;

Y-shaped flushing pipe 2, the bottom end of the flushing pipe 2 is connected with the connecting pipe 1, the top side of the flushing pipe 2 is connected with the peristaltic pump 6, and the water inlet of the peristaltic pump is connected with a centrifuge tube with a precise scale to control the amount of buffer used , the other end is connected with the syringe 7, and the syringe can also be replaced by a dropping funnel; the two branches of the Y-shaped flushing pipe are provided with a flow controller 11; Instead of the tube, the tip of the in-line flushing tube is connected to the peristaltic pump.

The intestinal fixer 3 includes a first clip 31 and a second clip 32 for clamping the connecting tube and the animal intestine, and a second clip 32 for adjusting the distance between the first clip 31 and the second clip 32. Adjuster; in this embodiment, the adjuster is a knob screw 33, the first clip 31 is arranged on the first fixing plate 34, the knob screw 33 is arranged on the second fixing plate 35, and the end of the knob screw 33 is connected with the first fixing plate 34. Two clips 32, the first clip 31 and the second clip 32 are arranged parallel to each other, and the distance between the two can be adjusted by rotating the knob screw, thereby fixing or loosening the animal intestine. The first clip 31 and the second clip 32 are provided with arc-shaped grooves 9 at the positions in contact with the intestinal tract.

The first fixing plate 34 and the second fixing plate 35 are both arranged on the fixing seat 36, the fixing seat 36 is fixed on the iron frame 4, the fixing seat 36 is also vertically provided with a third fixing plate 37, and the third fixing plate is connected with a A fixed rod 38 is fixed on the fixed rod 38 with a syringe and a flushing tube.

The liquid receiver 5 is located below the intestinal fixer. In this embodiment, the liquid receiver is a beaker. When the intestinal contents need to be collected, a centrifuge tube can be used instead of the beaker to receive the intestinal flushing. the liquid;

An iron stand 4, on which a liquid receiver is placed, and a bracket of the iron stand is used to fix the intestinal fixer.

The use process of the flushing device is as follows: the components of the flushing device are connected according to the above-mentioned positional relationship, a section of the intestinal tract of a mouse or a rat is selected as required, and the connecting tube is inserted into the upper end of the intestinal tract and then fixed on the first clip and the second clip. In the clip, then the irrigation tube is sealed with the connecting tube, and the peristaltic pump is turned on to rinse the intestines.

Example 2

As shown in FIGS. 3 and 4 , the difference from Embodiment 1 is that in this embodiment, the flushing pipe 2 is a straight-line flushing pipe, and the upper end of the flushing pipe 2 is connected to a dripping funnel 8 , and the dripping funnel 8 is fixed on an iron stand. The lower end of the flushing pipe is sealed and fixed with the connecting pipe. The structure of the intestinal fixer in this embodiment is as follows: the adjuster is an adjusting bolt 12 and an adjusting nut 13, the ends of the first clip 31 and the second clip 32 are connected by a rotating shaft, and a spring and an adjusting The bolt 12, the outer side of the first clip or the second clip is provided with an adjusting nut 13; the end of the first clip or the second clip is provided with a handle for fixing on the iron stand. The use process is the same as in Example 1.

The above are several technical solutions listed in the present invention, which are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims (8)

1. An ex vivo irrigation device for the intestinal tract of a laboratory animal, characterized in that it comprises:

one end of the connecting pipe (1) extends into the intestinal tract of the animal, and the other end of the connecting pipe (1) is connected with the flushing pipe (2); the connecting pipe (1) is conical, the height is 2-5 cm, and the diameter of the bottom is 0.2-0.8 cm;

one end of the flushing pipe (2) is connected with the connecting pipe, and the other end of the flushing pipe is connected with the liquid feeding device;

the intestinal canal fixer (3), the intestinal canal fixer (3) comprises a first clamping piece (31) and a second clamping piece (32) for clamping the connecting pipe and the intestinal canal of the animal, and a regulator for regulating the distance between the first clamping piece and the second clamping piece;

the liquid receiving device (5), the liquid receiving device (5) is positioned below the intestinal fixer;

the liquid receiving device is placed on the table top of the iron table stand (4), and the support of the iron table stand is used for fixing the intestinal fixer.

2. An in vitro irrigation apparatus for experimental animal intestinal tract according to claim 1, characterized in that the liquid feeding device is one or more of a peristaltic pump (6), a syringe (7) and a dropping funnel (8).

3. The device for the extracorporeal irrigation of the intestinal tract of a laboratory animal according to claim 1, characterized in that the irrigation tube (2) is a straight irrigation tube or a Y-shaped irrigation tube.

4. The extracorporeal irrigation device for the intestinal tract of a laboratory animal as set forth in claim 1, characterized in that the first jaw (31) and the second jaw (32) are provided with an arc-shaped groove (9) at a position contacting the intestinal tract.

5. The apparatus for ex vivo flushing of the intestinal tract of a laboratory animal of claim 1, wherein said flushing tube or liquid feeder is fixed to a brandreth.

6. The in vitro irrigation device for the intestinal tracts of laboratory animals according to any one of claims 1 to 5, wherein the regulator is a knob screw, the first clamping piece is arranged on a first fixing plate, the knob screw is arranged on a second fixing plate, the tail end of the knob screw is connected with a second clamping piece, and the first clamping piece and the second clamping piece are arranged oppositely;

the first fixing plate and the second fixing plate are both arranged on the fixing base, the fixing base is fixed on the iron support, and the fixing base is further connected with a third fixing plate and a fixing rod which are used for fixing the liquid feeding device and/or the flushing pipe.

7. The in vitro irrigation device for the intestinal tracts of experimental animals according to any one of claims 1 to 5, wherein the regulator is an adjusting bolt and an adjusting nut, the tail ends of the first clamping piece and the second clamping piece are connected through a rotating shaft, a spring and the adjusting bolt are arranged between the first clamping piece and the second clamping piece, and the adjusting nut is arranged on the outer side of the first clamping piece or the second clamping piece; the tail end of the first clamping piece or the second clamping piece is provided with a handle for being fixed on an iron support.

8. The in vitro irrigation apparatus for the intestinal tract of laboratory animals according to any one of claims 1 to 5, wherein the regulator is a spring clip, the first clip and the second clip are hinged together through the spring clip, the distance between the first clip and the second clip is adjusted by pressing the tail end of the first clip and/or the second clip, and the tail end of the first clip or the second clip is provided with a handle for fixing on an iron support.

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