CN210856148U - Electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation - Google Patents
Electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation Download PDFInfo
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- CN210856148U CN210856148U CN201921034223.4U CN201921034223U CN210856148U CN 210856148 U CN210856148 U CN 210856148U CN 201921034223 U CN201921034223 U CN 201921034223U CN 210856148 U CN210856148 U CN 210856148U
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Abstract
An electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation relates to the field of biological preparation production equipment. Including biological pre-reaction pressure vessel, biological reaction pressure vessel and gas-liquid mixer, the pulse magnetic field generator of relative arrangement is installed to both sides in the biological pre-reaction pressure vessel, and fixed mounting has electromagnetic wave generator in the biological reaction pressure vessel, and one side on biological reaction pressure vessel upper portion is provided with the inlet, and the opposite side on biological reaction pressure vessel upper portion is connected with the drain pipe, and bottom one side of biological reaction pressure vessel is provided with the backward flow mouth, and the drain pipe is connected with the entrance point of gas-liquid mixer, and the backward flow mouth of biological reaction pressure vessel is connected to the exit end of gas-liquid mixer. The utility model discloses can make culture medium biotransformation efficiency improve more than 1 times, throughput and finished product quality homoenergetic obtain improving, and the rainbow is inhaled the liquid blender and can be made the defoaming technology of whole microorganism growth in-process and realize zero interpolation.
Description
Technical Field
The utility model relates to the field of biological preparation production equipment, in particular to an electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation.
Background
Microbial fermentation refers to a process of converting raw materials into products required by human beings through a specific metabolic pathway by using microorganisms under appropriate conditions. The level of microbial fermentation production depends mainly on the genetic characteristics of the strain itself and the culture conditions. At present, the natto fermentation takes bacillus subtilis as a strain, only microbial raw materials are simply placed in a fermentation tank, the fermentation reaction is accelerated by common conventional means such as heating, stirring and the like, and the defect of low biotransformation efficiency exists.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation, which can effectively solve the problems in the background technology.
The technical scheme for realizing the purpose is as follows: an electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation is characterized in that: comprises a biological pre-reaction pressure vessel, a biological reaction pressure vessel and a gas-liquid mixer,
an electromagnetic wave generator is fixedly installed in the biological reaction pressure container, a first exhaust port is arranged at the top end of the biological reaction pressure container, the first exhaust port is connected with a second flow regulating valve, a liquid inlet is formed in one side of the upper portion of the biological reaction pressure container, a liquid outlet pipe is connected to the other side of the upper portion of the biological reaction pressure container, a backflow port is formed in one side of the bottom of the biological reaction pressure container, the liquid outlet pipe is connected with the inlet end of a gas-liquid mixer, and the outlet end of the gas-liquid mixer is connected with the backflow port;
the biological pre-reaction pressure vessel is characterized in that pulse magnetic field generators which are oppositely arranged are installed on two sides in the biological pre-reaction pressure vessel, a second exhaust port is arranged at the top end of the biological pre-reaction pressure vessel and connected with a third flow regulating valve, and the bottom end of the biological pre-reaction pressure vessel is connected with a liquid inlet of the biological reaction pressure vessel through a third control valve.
Furthermore, the electromagnetic wave generator is a TDP magic lamp, and the two electromagnetic wave generators are symmetrically arranged on two sides of the top of the biological reaction pressure vessel.
Furthermore, the liquid outlet pipe is provided with a plurality of liquid outlet pipes which are arranged up and down, and the outlet ends of the liquid outlet pipes are connected with the gas-liquid mixer through a fourth control valve after being mutually connected in parallel.
Further, be fixed with many vertical layout's siphon on the inner wall of biological reaction pressure vessel, fixed mounting has annular compressed air pipe in the biological reaction pressure vessel of the top of siphon, and annular compressed air pipe is connected with the intake pipe, and the intake pipe is connected compressed air source through first flow control valve, the first control valve that concatenates each other, still is connected with the jet-propelled pipe that extends to the upper port of siphon respectively on the annular compressed air pipe.
Furthermore, a first stirring device is arranged in the biological reaction pressure vessel.
Further, a second stirring device is arranged in the biological pre-reaction pressure container.
Furthermore, the biological pre-reaction pressure container is connected with a compressed air source through a second flow regulating valve and a second control valve which are mutually connected in series.
The utility model has the advantages that:
the utility model discloses during operation, the culture medium in the biological pre-reaction pressure vessel carries out the fermentation under the magnetic field environment of pulse magnetic field generator and expands and cultivate, expand and cultivate the back, biological culture medium reseedes to the biological reaction pressure vessel in, in the normal fermentation process, opens the electromagnetic wave generator in the biological reaction pressure vessel, improves the microorganism metabolic activity; compressed air is started, the compressed air reaches the bottom of the reaction container through the siphon pipe and then upwards conveys foam generated on the surface of the culture medium to the gas-liquid mixer in a siphoning mode quickly to be mixed, and then the foam is conveyed to the bottom of liquid in the biological reaction pressure container, so that the purpose of replacing chemical defoaming with physical circulation defoaming is realized, the cost is greatly saved, and the fermentation effect is ensured.
The arrangement of the magnetic field and the TDP magic lamp can improve the biotransformation efficiency of the culture medium by more than 1 time, both the production capacity and the finished product quality can be improved, and the siphon liquid mixer can realize zero addition of the defoaming process in the whole microorganism growth process.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of the distribution structure of siphons in the bioreactor pressure vessel.
Detailed Description
One, the technical scheme of the utility model
As shown in figures 1 and 2, the utility model discloses mainly be applied to the natto fermentation who uses the bacillus subtilis as the bacterial strain, including the volumetric biological pre-reaction pressure vessel 1 of 30L, the volumetric biological reaction pressure vessel 2 of 10000L and gas-liquid mixer 3, biological pre-reaction pressure vessel 1, biological reaction pressure vessel 2 are inclosed pressure vessel.
Two sides in the biological pre-reaction pressure vessel 1 are provided with pulse magnetic field generators 1.1 which are oppositely arranged, the top end of the biological pre-reaction pressure vessel 1 is provided with a second exhaust port 21, the second exhaust port 21 is connected with a third flow regulating valve 22, the magnetic field intensity is 0.5-1.5T, and the pulse number is 5-15.
An electromagnetic wave generator 4 is fixedly installed in the biological reaction pressure vessel 2, the electromagnetic wave generator 4 is a TDP magic lamp, two electromagnetic wave generators 4 are arranged and symmetrically installed on two sides of the top of the biological reaction pressure vessel 2, a first exhaust port 19 is arranged at the top end of the biological reaction pressure vessel 2, the first exhaust port 19 is connected with a second flow regulating valve 20, a liquid inlet 2.1 is arranged on one side of the upper part of the biological reaction pressure vessel 2, a liquid outlet pipe 5 is connected on the other side of the upper part of the biological reaction pressure vessel 2, a backflow port 2.2 is arranged on one side of the bottom of the biological reaction pressure vessel 2, the liquid inlet 2.1 of the biological reaction pressure vessel 2 is connected with the biological pre-reaction pressure vessel 1 through a third control valve 13, a plurality of liquid outlet pipes 5 are arranged up and down, the outlet ends of the liquid outlet pipes 5 are connected with each, the outlet end of the gas-liquid mixer 3 is connected with a reflux port 2.2 of the biological reaction pressure vessel 2.
Be fixed with many vertical layout's siphon 6 on the inner wall of biological reaction pressure vessel 2, fixed mounting has annular compressed air pipe 7 in the biological reaction pressure vessel 2 of the top of siphon 6, still be connected with the jet-propelled pipe 9 that extends to siphon 6's upper port respectively on the annular compressed air pipe 7, annular compressed air pipe 7 is connected with the intake pipe 8 that outwards stretches out biological reaction pressure vessel 2, intake pipe 8 is through the first flow control valve 14 that concatenates each other, compressed air source is connected to first control valve 15, compressed air source still is through the second flow control valve 16 that concatenates each other simultaneously, biological pre-reaction pressure vessel 1 is connected to second control valve 17.
Stirring devices are respectively arranged in the biological pre-reaction pressure container 1 and the biological reaction pressure container 2, each stirring device comprises a stirring shaft 10 respectively arranged in the biological pre-reaction pressure container 1 and the biological reaction pressure container 2, stirring blades 11 are arranged on the stirring shafts 10, and a driving motor 12 for driving the stirring shafts 10 to rotate is arranged at the top ends of the biological pre-reaction pressure container 1 and the biological reaction pressure container 2.
Secondly, taking the bacillus natto strain as an object, and testing the influence of the pulsed magnetic field generator 1.1 on microbial fermentation, wherein the test steps are as follows:
test example 1
1) Preparing a bacillus natto strain;
2) preparing an ion culture medium for producing nattokinase: weighing 180g of tryptone, 180g of glucose, 18g of yeast powder, 90g of beef extract powder and 90g of sodium chloride, putting the weighed materials into a biological pre-reaction pressure container 1, adding 14 kg of purified water, and uniformly stirring to obtain an ion culture medium for producing nattokinase;
3) inoculating the bacillus natto strain into the ion culture medium prepared in the step 2), placing the bacillus natto strain into a shake flask of an oscillator at 36-37 ℃, and carrying out shake culture for 8-12 h at 100-120 r/min to prepare a fermentation seed;
4) according to the inoculation ratio of 3-10%, the fermentation seeds are put into a biological pre-reaction pressure container 1, a second control valve 17 is opened, and the flow of a second flow control valve 16 is adjusted, so that the pressure in the biological pre-reaction pressure container 1 is 0.1MPa, and the air input per kilogram of fermentation liquor is 0.1m3Performing continuous fermentation culture for 24 hours in the environment with the magnetic field intensity of 0.5T and the pulse number of 5 and continuously stirring at the speed of 100 r/min;
5) sampling the fermentation liquor every two hours in the continuous fermentation process, and determining the activity value of the serine enzyme;
6) and after the fermentation is stopped, drying the fermented seeds within 24 h.
Test example two: the same procedure as in test example one, except that:
the fermentation process in the step (4) is carried out under the environment that the magnetic field intensity is 1T and the pulse number is 10;
test example three: the same procedure as in test example one, except that:
the fermentation process in the step 4) is carried out under the environment that the magnetic field intensity is 1.5T and the pulse number is 15;
test example four: the same procedure as in test example one, except that:
the fermentation process in the step 4) is carried out under the environment that the magnetic field intensity is 3.3T and the pulse number is 25;
test example five: the same procedure as in test example one, except that:
the fermentation process in the step 4) is carried out under the environment that the magnetic field intensity is 10T and the pulse number is 48;
table 1 activity values for the various embodiments at different magnetic field strengths:
as can be seen from Table 1: the magnetic field intensity and the pulse number have great influence on the growth effect of the bacillus subtilis, the bacillus subtilis is most sensitive to the pulse magnetic field in the logarithmic growth phase, the metabolism of the bacillus subtilis is reduced along with the increase of the magnetic field intensity (more than 1.5T) and the pulse number (more than 15), and the enzyme activity is in a descending trend; but when the magnetic field intensity (less than or equal to 1.5T) and the pulse number (less than or equal to 15) are relatively low, the pulse magnetic field plays a role in promoting the growth and accelerating the metabolism of the bacillus subtilis.
Thirdly, taking the bacillus natto strain as an object to test the influence of TDP magic lamp on microbial fermentation, and the test steps are as follows:
respectively with three test tubes, hold 0.2ml bacillus natto fungus liquid in each test tube, shine with TDP spirit lamp respectively, the irradiation distance is 30cm, and irradiance is 54 mW/square centimeter, shines 15min for each time, and 10 am and 4 pm are once respectively, and the incubator of 30 ℃ is put into after shining to cultivate, observes in the morning of the next day. The ambient temperature of the irradiation was 26 ℃. If the temperature of the test tube is too high, a rotary test tube method is adopted to achieve temperature balance.
OD (optical density absorbed by the object) values were measured, and after the irradiation treatment, the OD values of the bacterial solutions of each group were measured by an ultraviolet spectrophotometer, and the measurement results are shown in Table 2.
TABLE 2 OD value of Bacillus natto bacterial liquid after irradiation treatment
| Test tube numbering | 1 | 2 | 3 |
| OD value | 0.650 | 0.813 | 0.519 |
Note: the initial OD of the Bacillus natto bacterial liquid is 0.054, OD is lg (1/trans), wherein trans is the light transmission value of a detection object)
The research result of the experiment shows that: under the same conditions, TDP headlight illumination has a unique biological effect. There is an important prerequisite that the electromagnetic waves radiated by the TDP must be absorbed by the body before they can act on the body. The growth of the microorganism needs nutrient elements such as a carbon source, a nitrogen source, an energy source, inorganic salt and the like, and the TDP magic lamp can activate the activity of macromolecules in the microorganism, so that the molecules of the microorganism can be excited to be in a high vibration state, and the activity of macromolecules such as protein and the like is activated. The enzyme is a water-soluble protein with catalytic action made by living cells, is composed of enzyme protein and accessory factor, and is characterized in that one enzyme can only act on one kind of substrate. Beneficial and harmless specific electromagnetic waves to microorganisms can promote the change of an enzyme system in the microorganisms, increase enzyme metabolism, improve the activity of the enzyme and increase growth factors, thereby promoting the growth, development, reproduction and repair of organism tissues.
Fourthly, the bacillus natto strain is used as an object to test the influence of the technical scheme of the embodiment on the microbial fermentation, and the test steps are as follows:
1) preparing a bacillus natto strain;
2) weighing 180g of tryptone, 180g of glucose, 18g of yeast powder, 90g of beef extract powder and 90g of sodium chloride according to the following components by mass, placing the weighed materials in a biological pre-reaction pressure container 1, adding 14L of purified water, and uniformly stirring to obtain an ion culture medium for producing nattokinase;
3) 250kg of kidney bean powder, 100kg of glucose, 25kg of sodium chloride, 2.5kg of sodium hydroxide, 0.15kg of magnesium sulfate, 0.35kg of calcium chloride, 2.5kg of sodium hydroxide, 150g of tween (80), 1.5kg of potassium dihydrogen phosphate and 20kg of disodium hydrogen phosphate are weighed according to the following components by mass and placed in a biological reaction pressure container 2, and 5000L of purified water is added and stirred uniformly to obtain the ionic culture medium.
4) Inoculating the bacillus natto strain into the ion culture medium prepared in the step 2), placing the bacillus natto strain into a shake flask of an oscillator at 36-37 ℃, and carrying out shake culture at 100-120 r/min for 8-12 h to prepare a fermentation seed;
5) according to the inoculation ratio of 3-10%, putting fermentation seeds into the biological pre-reaction pressure container 1 in the step 2), opening the second control valve 17, adjusting the flow of the second flow control valve 16, adjusting the pressure in the biological pre-reaction pressure container 1 to be 0.06MPa, and adjusting the air input per kilogram of fermentation liquor to be 0.1m3Regulating a pulsed magnetic field generator 1.1, controlling the magnetic field intensity to be 1.4T and the pulse number to be 14, and stirring at the speed of 400r/min for continuous fermentation culture for 12 hours;
5) after the culture expansion is finished, opening a third control valve 13, inoculating the expanded bacterial liquid into the biological reaction pressure container 2, opening a first control valve 15, adjusting the pressure in the biological reaction pressure container 2 to be 0.05MPa through a first flow regulating valve 14 and a second flow regulating valve 20, and ensuring that the air input in the container is 10m3Min, displacement 6.5m3And/min, the stirring speed of the biological reaction pressure vessel 2 is kept between 80 and 120 r/min.
6) And (3) turning on the TDP magic lamp when the fermentation is normally carried out for 4 hours, continuously irradiating the surface of the fermentation liquor for 30min, then turning off the fermentation liquor, respectively turning on the TDP magic lamp when the fermentation is carried out for 8 hours, 12 hours, 16 hours and 20 hours, and continuously irradiating the surface of the fermentation liquor for 30 min.
7) In the fermentation process, if the foam height is too high, the fourth control valve 18 is opened, the upper foam enters the gas-liquid mixer 7 from the liquid outlet pipe 5 under the action of compressed air, is mixed and then enters the biological reaction pressure container 2 from the backflow port 2.2, and accordingly circular defoaming in a physical mode is achieved.
8) Sampling the fermentation liquor every two hours in the continuous fermentation process, sampling in batches for four times, and determining the nattokinase activity value;
9) after the fermentation is stopped, drying treatment is carried out within 24 h.
The final enzyme activities of the four batches are shown in table 3:
| batches of | Enzyme activity (U/g) |
| 1 | 158200 |
| 2 | 165378 |
| 3 | 158653 |
| 4 | 169621 |
| Conventional fermentation | 78232 |
TABLE 3
And (4) conclusion: the arrangement of the electromagnetic waves can improve the biotransformation efficiency of the culture medium by more than 1 time, both the production capacity and the finished product quality can be improved, and the siphon gas-liquid mixer can realize zero addition of the defoaming process in the whole microorganism growth process.
Claims (7)
1. An electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation is characterized in that: comprises a biological pre-reaction pressure vessel, a biological reaction pressure vessel and a gas-liquid mixer,
an electromagnetic wave generator is fixedly installed in the biological reaction pressure container, a first exhaust port is arranged at the top end of the biological reaction pressure container, the first exhaust port is connected with a second flow regulating valve, a liquid inlet is formed in one side of the upper portion of the biological reaction pressure container, a liquid outlet pipe is connected to the other side of the upper portion of the biological reaction pressure container, a backflow port is formed in one side of the bottom of the biological reaction pressure container, the liquid outlet pipe is connected with the inlet end of a gas-liquid mixer, and the outlet end of the gas-liquid mixer is connected with the backflow port;
the biological pre-reaction pressure vessel is characterized in that pulse magnetic field generators which are oppositely arranged are installed on two sides in the biological pre-reaction pressure vessel, a second exhaust port is arranged at the top end of the biological pre-reaction pressure vessel and connected with a third flow regulating valve, and the bottom end of the biological pre-reaction pressure vessel is connected with a liquid inlet of the biological reaction pressure vessel through a third control valve.
2. The electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation as claimed in claim 1, wherein: the electromagnetic wave generator is a TDP magic lamp, and the two electromagnetic wave generators are symmetrically arranged on two sides of the top of the biological reaction pressure vessel.
3. The electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation as claimed in claim 1, wherein: the liquid outlet pipe is provided with a plurality of liquid outlet pipes which are arranged up and down, and the outlet ends of the liquid outlet pipes are connected with a gas-liquid mixer through a fourth control valve after being mutually connected in parallel.
4. The electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation as claimed in claim 1, wherein: be fixed with many vertical layout's siphon on biological reaction pressure vessel's the inner wall, fixed mounting has annular compressed air pipe in the biological reaction pressure vessel of the top of siphon, and annular compressed air pipe is connected with the intake pipe, and the compressed air source is connected through the first flow control valve, the first control valve that concatenate each other to the intake pipe, still is connected with the jet-propelled pipe that extends to the upper port of siphon respectively on the annular compressed air pipe.
5. The electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation as claimed in claim 4, wherein: a first stirring device is arranged in the biological reaction pressure container.
6. The electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation as claimed in claim 1, wherein: and a second stirring device is arranged in the biological pre-reaction pressure container.
7. The electromagnetic wave siphon self-defoaming biological reaction device for natto fermentation as claimed in claim 6, wherein: the biological pre-reaction pressure container is connected with a compressed air source through a second flow regulating valve and a second control valve which are mutually connected in series.
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| CN110184186A (en) * | 2019-07-04 | 2019-08-30 | 舒禾江苏健康产业有限公司 | A kind of electromagnetic wave siphon self-defoaming biological reaction apparatus for natto fermentation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110184186A (en) * | 2019-07-04 | 2019-08-30 | 舒禾江苏健康产业有限公司 | A kind of electromagnetic wave siphon self-defoaming biological reaction apparatus for natto fermentation |
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Effective date of registration: 20211130 Address after: 225300 No. 5, standard workshop, Lingang Economic Park, Gaogang District, Taizhou City, Jiangsu Province Patentee after: Jiangsu Yikang Health Industry Co.,Ltd. Address before: 225300 Standard Workshop of Lingang Economic Park, Gaogang District, Taizhou City, Jiangsu Province Patentee before: SHUHE JIANGSU HEALTH INDUSTRY CO.,LTD. Patentee before: Lu Xiao |