CN210134121U

CN210134121U - Culture apparatus of effective microbiota active solution

Info

Publication number: CN210134121U
Application number: CN201920469105.XU
Authority: CN
Inventors: 李瑶
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-04-08
Filing date: 2019-04-08
Publication date: 2020-03-10
Anticipated expiration: 2029-04-08

Abstract

The utility model relates to a culture device of effective microbiota active liquid, which comprises a first strain activation tank, a second strain activation tank, a culture liquid tank, a biological culture tank and a main fermentation tank, wherein the first strain activation tank, the second strain activation tank, the culture liquid tank and the biological culture tank are all provided with a feed port and a finished product discharge port, and each discharge port is connected with the feed port; an aeration oxygen supply mechanism and a dissolved oxygen monitoring mechanism are arranged in the first strain activation tank, a centrifugal cylinder is arranged in the first strain activation tank, sieve pores are distributed on the side wall of the centrifugal cylinder, and a centrifugal motor for driving the centrifugal cylinder is fixedly arranged at the bottom of the first strain activation tank; a first stirring mechanism is arranged on the culture solution tank; the biological culture tank is arranged on the shaking table, and a light source plate is arranged in the biological culture tank; the main fermentation tank is placed in the constant-temperature water bath box, a second stirring mechanism and a pH regulating mechanism are arranged in the main fermentation tank, and an exhaust port is formed in the top of the main fermentation tank. The utility model discloses can obviously improve work efficiency and bacterial survival rate, and the simple operation, labour saving and time saving, greatly reduced working strength.

Description

Culture apparatus of effective microbiota active solution

Technical Field

The utility model relates to an effective microbiota cultivates and handles technical field, more specifically says, relates to a culture apparatus of effective microbiota active solution.

Background

Effective microbiota (EM bacteria for short) is used as the most common composite microecological preparation, aerobic microorganisms, anaerobic microorganisms and facultative microorganisms are organically combined together through scientific screening, proper quantity ratio relation and reasonable fermentation process to form a stable multifunctional microecological system, and the multifunctional microecological system has the effects of degrading pollution, purifying water quality, stabilizing water ecological system balance and improving the ecological regulation function. At present, the culture of EM bacterial liquid mostly adopts a mode of carrying out totally-closed plastic barrel culture by taking mixed strains as zymogens and a standing anaerobic culture mode of large plastic bags and glass jar containers, but the control of effective culture conditions and the control of sterilization measures are not available in the culture process, the pollution degree of mixed bacteria is large, the growth of dominant strains is slow, and the culture is easy to fail; and for each single strain, the strain is mixed with other strains for fermentation without complete culture and propagation, so that the synergistic fermentation effect among the strains can not be exerted, the growth and propagation rate and the culture density of the strains are influenced, and the number of effective viable bacteria of the EM bacterial liquid is greatly reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problem that a culture apparatus of effective microbiota active fluid is provided, work efficiency and bacterial survival rate can obviously be improved, and the simple operation, labour saving and time saving, greatly reduced working strength.

In order to solve the technical problem, the utility model provides a culture device of effective microbiota active liquid, which comprises a first strain activation tank, a second strain activation tank, a culture solution tank, a biological culture tank, a main fermentation tank and a main control box;

the top of the first strain activation tank is provided with a first feed port, the first feed port is provided with a first feed valve, the bottom of the first strain activation tank is provided with a first discharge port, the first discharge port is provided with a first discharge valve, the first strain activation tank is internally provided with an aeration oxygen supply mechanism and a dissolved oxygen monitoring mechanism for detecting the dissolved oxygen amount of a solution, the first strain activation tank is internally provided with a centrifugal cylinder, the outlet of the first feed port is opposite to the centrifugal cylinder, the side wall of the centrifugal cylinder is uniformly distributed with a plurality of sieve pores, the bottom of the first strain activation tank is fixedly provided with a centrifugal motor, and the driving shaft of the centrifugal motor is connected with the centrifugal cylinder;

a second feed port is formed in the top of the second strain activation tank, a second feed valve is arranged on the second feed port, a second discharge port is formed in the bottom of the second strain activation tank, and a second discharge valve is arranged on the second discharge port;

a third feeding hole is formed in the top of the culture liquid tank, a third feeding valve is arranged on the third feeding hole, a third discharging hole is formed in the bottom of the culture liquid tank, a third discharging valve is arranged on the third discharging hole, and a first stirring mechanism is arranged on the culture liquid tank;

the biological culture tank is arranged on the shaking table, a fourth feed port is formed in the top of the biological culture tank, a fourth feed valve is arranged on the fourth feed port, a fourth discharge port is formed in the bottom of the biological culture tank, a fourth discharge valve is arranged on the fourth discharge port, and a light source plate is arranged in the biological culture tank;

a feeding port is formed in the top of the main fermentation tank, a fifth feeding valve is arranged on the feeding port, the feeding port is connected with the first discharge port, the second discharge port, the third discharge port and the fourth discharge port, a finished product discharge port is formed in the bottom of the main fermentation tank, and a fifth discharge valve is arranged on the finished product discharge port; the main fermentation tank is placed in a constant-temperature water bath tank, a second stirring mechanism is arranged in the main fermentation tank, a pH regulating mechanism is arranged in the main fermentation tank, an exhaust port is formed in the top of the main fermentation tank, and an exhaust switch valve is arranged on the exhaust port;

the main control box is respectively electrically connected with the aeration oxygen supply mechanism, the dissolved oxygen monitoring mechanism, the centrifugal motor, the first stirring mechanism, the oscillating table, the light source plate, the constant-temperature water bath box, the second stirring mechanism, the pH regulation mechanism, the first feed valve, the first discharge valve, the second feed valve, the second discharge valve, the third feed valve, the third discharge valve, the fourth feed valve, the fourth discharge valve, the fifth feed valve, the fifth discharge valve and the exhaust switch valve.

As the preferred scheme of the utility model, the upper portion of first bacterial activation jar is equipped with the trickle mechanism, the trickle mechanism with the main control box electricity is connected.

As the preferred proposal of the utility model, the number of the first strain activation tanks is 2.

As the preferred proposal of the utility model, the number of the biological culture tanks is 2.

In a preferred embodiment of the present invention, the light source plate in one of the biological growth pots has a wavelength of 560nm to 620nm, and the light source plate in the other of the biological growth pots has a wavelength of 420nm to 480 nm.

As the preferred proposal of the utility model, 2 the illumination intensity of the light source plate in the biological culture tank is 3000 Lux-6000 Lux.

As the utility model discloses preferred scheme, the end of giving vent to anger of gas vent is equipped with the ultraviolet lamp, the ultraviolet lamp with the main control box electricity is connected.

As the utility model discloses preferred scheme, be equipped with temperature-detecting mechanism in the main fermentation jar, temperature-detecting mechanism with the main control box electricity is connected.

As the utility model discloses preferred scheme, the upper portion of main fermentation cylinder is equipped with cooling trickle mechanism, cooling trickle mechanism with the main control box electricity is connected.

As the utility model discloses preferred scheme, first bacterial activation jar, second bacterial activation jar, culture solution jar, shaking table all set up on culture platform, the constant temperature water bath sets up on fermentation platform, culture platform's position is higher than fermentation platform's position.

Implement the utility model discloses a culture apparatus of effective microbiota active fluid, compare with prior art, have following beneficial effect:

the utility model discloses a first bacterial activation jar improves the dissolved oxygen volume in the first bacterial activation jar through aeration oxygen suppliment mechanism, makes the aerobic microorganism in the first bacterial activation jar activate and breeds the cultivation, and during the operation of first bacterial activation jar, makes the centrifuge bowl make high-speed rotation through centrifugal motor, leans on centrifugal force to throw the fungus liquid that has activated outside the centrifuge bowl through the sieve mesh, makes the fungus liquid flow to the bottom along the bucket wall of first bacterial activation jar, plays the separation action, realizes aerobic cultivation in first bacterial activation jar; performing closed culture in a second strain activation tank to activate anaerobic microorganisms in the second strain activation tank and perform propagation culture, and performing anaerobic culture in the second strain activation tank; uniformly stirring various nutrients in the culture solution tank through a first stirring mechanism; the light source plate of the biological culture tank is illuminated to culture and proliferate microorganisms in the biological culture tank, and the biological culture tank is arranged on the shaking table to fully stir the solution, so that the microbial strains are uniformly illuminated to keep the temperature balanced, the adherence and agglomeration phenomena in the culture process are effectively prevented, and the full utilization rate of the light source and the utilization rate of the substrate are ensured; the method comprises the following steps of firstly, respectively carrying out enrichment culture on microorganisms in a first strain activation tank, a second strain activation tank and a biological culture tank, putting various microorganisms subjected to enrichment culture and culture solution in the culture tank into a main fermentation tank for mixed strain culture, stirring the solution in the main fermentation tank through a second stirring mechanism, ensuring the culture temperature of the main fermentation tank through a constant-temperature water bath box, ensuring the solution in the main fermentation tank to be at the optimum culture pH value through a pH regulation mechanism, better playing the synergistic fermentation effect among strains and improving the growth and propagation speed of the strains; the utility model discloses can obviously improve work efficiency and bacterial survival rate, and the simple operation, labour saving and time saving, greatly reduced working strength.

Drawings

In order to more clearly illustrate the technical solution of the embodiments of the present invention, the drawings of the embodiments will be briefly described below.

FIG. 1 is a schematic diagram of the structure of a culture apparatus for an active liquid of an effective microorganism group;

wherein, 1 is a first strain activation tank; 11 is a first feed inlet; 12 is a first discharge hole; 13 is an aeration oxygen supply mechanism; 14 is a dissolved oxygen monitoring mechanism; 15 is a centrifugal cylinder; 16 is a sieve pore; 17 is a centrifugal motor; 18 is a water spraying mechanism; 2 is a second strain activation tank; 21 is a second feed port; 22 is a second discharge hole; 3 is a culture solution tank; 31 is a third feed inlet; 32 is a third discharge hole; 33 is a first stirring mechanism; 4 is a biological culture tank; 41 is an oscillating table; 42 is a fourth feed port; 43 is a fourth discharge hole; 44 is a light source plate; 5 is a main fermentation tank; 51 is a first feed port; 52 is a second feed port; 53 is a third feed port; 54 is a finished product discharge port; 55 is a second stirring mechanism; 56 is a pH regulating mechanism; 57 is an exhaust port; 58 is a temperature detection mechanism; 59 is a cooling water spraying mechanism; 6 is a constant temperature water bath box; 7 is an ultraviolet lamp; 8 is a culture platform; 9 is a fermentation platform; the main control box is 10.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in fig. 1, the preferred embodiment of the present invention is a culture apparatus for effective microbial population active solution, which comprises a first strain activation tank 1, a second strain activation tank 2, a culture solution tank 3, a biological culture tank 4, a main fermentation tank 5 and a main control box 10; the top of the first strain activation tank 1 is provided with a first feed inlet 11, the first feed inlet 11 is provided with a first feed valve, a first discharge hole 12 is arranged at the bottom of the first strain activation tank 1, a first discharge valve is arranged on the first discharge hole 12, an aeration oxygen supply mechanism 13 and a dissolved oxygen monitoring mechanism 14 for detecting the dissolved oxygen amount of the solution are arranged in the first strain activation tank 1, and the dissolved oxygen is controlled between 1.5mg/L and 3.0mg/L, a centrifugal cylinder 15 is arranged in the first strain activation tank 1, the outlet of the first feeding hole 11 is opposite to the centrifugal cylinder 15, a plurality of sieve holes 16 are uniformly distributed on the side wall of the centrifugal cylinder 15, a centrifugal motor 17 is fixedly arranged at the bottom of the first strain activation tank 1, a driving shaft of the centrifugal motor 17 is connected with the centrifugal cylinder 15, and the speed of the driving shaft of the centrifugal motor 17 is 820 r/min-900 r/min; wherein the activation time in the first strain activation tank 1 is 1.5-3 h, the tank body of the first strain activation tank 1 is cuboid, the centrifugal cylinder 15 is cylindrical, and the distance between the outer wall of the centrifugal cylinder 15 and the inner wall of the first strain activation tank 1 is 8 cm; a second feed inlet 21 is formed in the top of the second strain activation tank 2, a second feed valve is arranged on the second feed inlet 21, a second discharge outlet 22 is formed in the bottom of the second strain activation tank 2, and a second discharge valve is arranged on the second discharge outlet 22; the top of the culture solution tank 3 is provided with a third feeding port 31, the third feeding port 31 is provided with a third feeding valve, the bottom of the culture solution tank 3 is provided with a third discharging port 32, the third discharging port 32 is provided with a third discharging valve, the culture solution tank 3 is provided with a first stirring mechanism 33, wherein the first stirring mechanism 33 comprises a stirring paddle and a stirring motor, the stirring motor is fixed on the top of the culture solution tank 3, the stirring paddle is connected with a driving shaft of the stirring motor, the stirring speed is 80 r/min-150 r/min, and the stirring time is 10 min; the biological culture tank 4 is arranged on a shaking table 41, a fourth feed port 42 is arranged at the top of the biological culture tank 4, a fourth feed valve is arranged on the fourth feed port 42, a fourth discharge port 43 is arranged at the bottom of the biological culture tank 4, a fourth discharge valve is arranged on the fourth discharge port 43, a light source plate 44 is arranged in the biological culture tank 4, the light source plate 44 is preferably a waterproof LED light source plate 44, the shaking table 41 is preferably a reciprocating shaking table 41, the working frequency of the reciprocating shaking table 41 is 1 to 3 times per day, and the shaking time is 0.5 to 1.2min each time; a feeding port is formed in the top of the main fermentation tank 5, a fifth feeding valve is arranged on the feeding port, the feeding port is connected with the first discharge hole 12, the second discharge hole 22, the third discharge hole 32 and the fourth discharge hole 43, a finished product discharge hole 54 is formed in the bottom of the main fermentation tank 5, and a fifth discharge valve is arranged on the finished product discharge hole 54; the main fermentation tank 5 is placed in a constant-temperature water bath tank 6, the constant-temperature water bath tank 6 controls the temperature in the main fermentation tank 5 to be 28-35 ℃, a second stirring mechanism 55 is arranged in the main fermentation tank 5, the working frequency of the second stirring mechanism 55 is 1 time/day, the stirring speed is 20 rr/min-30 r/min, the stirring time is 0.5 min/time-2 min/time, the second stirring mechanism 55 comprises a stirring paddle and a stirring motor, the stirring motor is fixed at the top of the main fermentation tank 5, the stirring paddle is connected with a driving shaft of the stirring motor, a pH regulating mechanism 56 is arranged in the main fermentation tank 5, the pH regulating mechanism 56 controls the pH value in the main fermentation tank 5 to be 3.0-3.8, and an acidic solution and an alkaline solution for regulating the pH value in the main fermentation tank 5 are arranged in the pH regulating mechanism 56; an exhaust port 57 is formed in the top of the main fermentation tank 5, an exhaust switch valve is arranged on the exhaust port 57, and the exhaust frequency of the exhaust port 57 is 1 time/day; the main control box 10 is respectively electrically connected with the aeration oxygen supply mechanism 13, the dissolved oxygen monitoring mechanism 14, the centrifugal motor 17, the first stirring mechanism 33, the oscillating table 41, the light source plate 44, the constant temperature water bath box 6, the second stirring mechanism 55, the pH regulation mechanism 56, the first feed valve, the first discharge valve, the second feed valve, the second discharge valve, the third feed valve, the third discharge valve, the fourth feed valve, the fourth discharge valve, the fifth feed valve, the fifth discharge valve and the exhaust switch valve.

The utility model discloses a first bacterial activation jar 1 improves the dissolved oxygen volume in the first bacterial activation jar 1 through aeration oxygen suppliment mechanism 13, makes the aerobic microorganism activation and the enrichment culture in the first bacterial activation jar 1, and when first bacterial activation jar 1 worked, makes centrifugal cylinder 15 make high-speed rotatory through centrifugal motor 17, leans on centrifugal force to throw activated fungus liquid outside centrifugal cylinder 15 through sieve mesh 16, makes the fungus liquid flow to the bottom along the bucket wall of first bacterial activation jar 1, plays the separation effect, realizes aerobic culture at first bacterial activation jar 1; performing closed culture in the second strain activation tank 2 to activate anaerobic microorganisms in the second strain activation tank 2 and perform propagation culture, and performing anaerobic culture in the second strain activation tank 2; various nutrients in the culture solution tank 3 are uniformly stirred by the first stirring mechanism 33; the light source plate 44 of the biological culture tank 4 is illuminated to culture and proliferate microorganisms in the biological culture tank 4, and the biological culture tank 4 is arranged on the shaking table 41 to fully stir the solution, so that the microorganism strains uniformly receive illumination, the temperature is kept balanced, the adherence and agglomeration phenomena in the culture process are effectively prevented, and the full utilization rate of the light source and the utilization rate of the substrate are ensured; firstly, microorganisms in a first strain activation tank 1, a second strain activation tank 2 and a biological culture tank 4 are respectively subjected to enrichment culture, various microorganisms subjected to enrichment culture and culture solution in a culture solution tank 3 are placed in a main fermentation tank 5 for mixed strain culture, the solution in the main fermentation tank 5 can be stirred by a second stirring mechanism 55, the culture temperature of the main fermentation tank 5 is ensured by a constant-temperature water bath tank 6, the solution in the main fermentation tank 5 is ensured to be at the optimum culture pH value by a pH regulation and control mechanism 56, the synergistic fermentation effect among strains is better played, and the growth and propagation speed of the strains is improved; the utility model discloses can obviously improve work efficiency and bacterial survival rate, and the simple operation, labour saving and time saving, greatly reduced working strength.

Illustratively, a water spraying mechanism 18 is arranged at the upper part of the first strain activation tank 1, the water spraying mechanism 18 is electrically connected with the main control box 10, the water spraying mechanism 18 is used for spraying water in a rotating manner, and the water spraying mechanism 18 washes the inner wall of the first strain activation tank 1 and the centrifugal cylinder 15 after the centrifugal cylinder 15 rotates and centrifuges.

Illustratively, the number of the first strain activation tanks 1 is 2, and the number of the biological culture tanks 4 is 2; wherein, the feeding port of the main fermentation tank 5 comprises a first feeding port 51, a second feeding port 52 and a third feeding port 53, the first discharge port 12 of each of the 2 first strain activation tanks 1 and the second discharge port 22 of each of the second strain activation tanks 2 are both in sealing connection with the first feeding port 51, the third discharge port 32 of the culture solution tank 3 is in sealing connection with the second feeding port 52, the fourth discharge port 43 of each of the 2 biological cultivation tanks 4 is both in sealing connection with the third feeding port 53, and the first feeding port 11, the second feeding port 21, the third feeding port 31, the fourth feeding port 42, the first feeding port 51, the second feeding port 52 and the third feeding port 53 are all provided with silica gel sealing rings.

Illustratively, the number of the biological culture tanks 4 is 2, the wavelength of the light source plate 44 in one biological culture tank 4 is 560nm to 620nm, the wavelength of the light source plate 44 in the other biological culture tank 4 is 420nm to 480nm, the illumination intensity of the light source plate 44 in 2 biological culture tanks 4 is 3000Lux to 6000Lux, and the light source plates 44 with different wavelengths are respectively arranged in 2 biological culture tanks 4, so that different photosynthetic microorganisms can be irradiated with the appropriate illumination wavelength.

In the opening sequence of the tanks of the culture apparatus for the effective microbiota active solution, the 1 st first strain activation tank 1 is opened first to enter the main fermentation tank 5, the 2 nd first strain activation tank 1 is opened after the enrichment culture is performed in the main fermentation tank 5 for 2 hours, the second strain activation tank 2 and the culture solution tank 3 are opened simultaneously after 2 hours, and the 2 biological culture tanks 4 are opened simultaneously after 15 hours.

Illustratively, an ultraviolet lamp 7 is arranged at the air outlet end of the air outlet 57, the ultraviolet lamp 7 is electrically connected with the main control box 10, and when the main fermentation tank 5 exhausts air, the sterilization is performed by opening the ultraviolet lamp 7, so that the influence on the effect caused by the entering of external bacteria from the air outlet 57 in the air exhaust process is avoided.

Exemplarily, a temperature detection mechanism 58 is arranged in the main fermentation tank 5, the temperature detection mechanism 58 is electrically connected with the main control box 10, a cooling and water spraying mechanism 59 is arranged on the upper portion of the main fermentation tank 5, and the cooling and water spraying mechanism 59 is electrically connected with the main control box 10. When the temperature detection mechanism 58 detects that the temperature of the solution in the main fermentation tank 5 is affected by the outside air temperature and is higher than 38 ℃ in summer, the temperature reduction and water spraying mechanism 59 is started to properly reduce the temperature to 28-35 ℃ to reach the optimal fermentation temperature.

Illustratively, the first strain activation tank 1, the second strain activation tank 2, the culture solution tank 3 and the shaking table 41 are all arranged on the culture platform 8, the constant-temperature water bath box 6 is arranged on the fermentation platform 9, and the position of the culture platform 8 is at least 50cm higher than that of the fermentation platform 9, so that the solution in the first strain activation tank 1, the second strain activation tank 2, the culture solution tank 3 and the biological culture tank 4 flows into the main fermentation tank 5 under the action of gravity.

The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the invention, therefore, the invention is not limited thereto.

Claims

1. A culture device for effective microbiota active liquid is characterized by comprising a first strain activation tank, a second strain activation tank, a culture liquid tank, a biological culture tank, a main fermentation tank and a main control box;

2. The apparatus for culturing an active microorganism group liquid according to claim 1, wherein a water spraying means is provided on the upper part of the first microorganism activating tank, and the water spraying means is electrically connected to the main control box.

3. The apparatus for culturing an active microbial population as in claim 1, wherein there are 2 first strain activation tanks.

4. The apparatus of claim 1, wherein there are 2 biological growth tanks.

5. The culture apparatus for an active microbial population solution according to claim 4, wherein the light source plate in one of said biological culture tanks has a wavelength of 560nm to 620nm, and the light source plate in the other of said biological culture tanks has a wavelength of 420nm to 480 nm.

6. The culture apparatus for effective microbiota active fluid of claim 4, wherein the light source plates in 2 of said biological culture tanks are each illuminated at 3000Lux to 6000 Lux.

7. The apparatus of claim 1, wherein an ultraviolet lamp is provided at the outlet end of the outlet port, the ultraviolet lamp being electrically connected to the main control box.

8. The apparatus of claim 1, wherein a temperature sensing means is provided in the main fermentor, and the temperature sensing means is electrically connected to the main control box.

9. The apparatus for culturing an active microbial population solution according to claim 8, wherein a temperature-reducing water-spraying mechanism is provided at the upper part of the main fermentation tank, and the temperature-reducing water-spraying mechanism is electrically connected to the main control box.

10. The apparatus of claim 1, wherein the first and second species activation tanks, the culture tank, and the shaker are all disposed on a culture platform, and the constant temperature water bath tank is disposed on a fermentation platform, the culture platform being located at a higher position than the fermentation platform.