CN210085443U - Umbilical cord stem cell extraction element - Google Patents
Umbilical cord stem cell extraction element Download PDFInfo
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- CN210085443U CN210085443U CN201821836552.6U CN201821836552U CN210085443U CN 210085443 U CN210085443 U CN 210085443U CN 201821836552 U CN201821836552 U CN 201821836552U CN 210085443 U CN210085443 U CN 210085443U
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- bottle
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- crushing
- umbilical cord
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Abstract
The utility model discloses an umbilical cord stem cell extraction device, which comprises a crushing bottle, a reaction extraction bottle and a placing bottle, wherein the crushing bottle is communicated with the interior of the reaction extraction bottle and is integrally formed from top to bottom, and the placing bottle is arranged at the lower side of the reaction extraction bottle; the placing bottle is connected with the reaction extracting bottle through a filter fixing piece; a double-layer filter screen is arranged in the filter fixing piece; the bottleneck department of reaction extraction bottle and placing bottle all is provided with the screw thread, reaction extraction bottle and placing bottle with filter mounting screw thread fixed connection. The utility model provides an umbilical cord stem cell extraction element can be so that the extract after drawing directly shifts, and contactless air has reduced manual operation's step, has avoided receiving the infringement of bacterium at the transfer in-process simultaneously, and extraction element and strorage device can separate, convenient washing is disinfected.
Description
Technical Field
The utility model relates to a stem cell draws technical field, concretely relates to umbilical cord stem cell extraction element.
Background
Mesenchymal Stem Cells (MSCs) are a class of stem cells in the bone marrow with high self-renewal and multipotent differentiation potential, in addition to Hematopoietic Stem Cells (HSCs). Under different induction conditions, the tissue can be differentiated into various tissue cells and has the functions of immunoregulation, tissue repair and the like. In the past, mesenchymal stem cells mainly come from bone marrow, but with aging, the mesenchymal stem cells are gradually reduced, the proliferation and differentiation capacity is greatly reduced, the material selection has damage to donors, and the application of the mesenchymal stem cells is limited. The search for a new alternative source is an urgent problem to be solved.
The placenta stem cells scientifically discovered at present comprise: placenta mesenchymal stem cells, placenta sub totipotent stem cells and placenta hematopoietic stem cells. The placental stem cells are stem cells obtained by collection of placental tissue. When a newborn is born, stem cells can be obtained through three channels. The first is the collection of hematopoietic stem cells from a source of blood within the cord. The second is the collection of umbilical cord-derived mesenchymal stem cells within the umbilical cord itself. And the third method is that placenta stem cells are collected from a placenta source. Usually, the stem cell extraction in the umbilical cord adopts Phosphate Buffer Solution (PBS) surface washing to remove blood stain, the clean umbilical cord is cut into pieces and then soaked in collagenase for treatment, then trypsinization treatment is carried out, and the liquid phase is centrifuged to obtain the target stem cell.
Among the prior art, the extraction and the whole relatively more complicacy of transfer device of placenta, manual operation is more, and the placenta carries out the in-process that shifts after extracting, and the contact air leads to the invasion of bacterium easily, is polluted easily, and then is unfavorable for the use and the research in later stage.
SUMMERY OF THE UTILITY MODEL
The utility model provides an umbilical cord stem cell extraction element can be so that the extract after drawing directly shifts, and contactless air has reduced manual operation's step, has avoided receiving the infringement of bacterium at the transfer in-process simultaneously, and extraction element and strorage device can separate, convenient washing is disinfected.
In order to achieve the above object, the present invention provides the following technical solutions:
an umbilical cord stem cell extraction device comprises a crushing bottle, a reaction extraction bottle and a placing bottle, wherein the crushing bottle is communicated with the interior of the reaction extraction bottle and is integrally formed from top to bottom, and the placing bottle is arranged at the lower side of the reaction extraction bottle; the placing bottle is connected with the reaction extracting bottle through a filtering fixing piece; a double-layer filter screen is arranged in the filter fixing piece; the bottleneck department of reaction extraction bottle and placing bottle all is provided with the screw thread, reaction extraction bottle and placing bottle with filter mounting screw thread fixed connection.
The utility model discloses in, preferred, smash the bottle include the bottle and with bottle threaded connection's bottle lid, be provided with an opening and shutting door on the bottle, the fixed crushing sword that is provided with on the diapire of bottle lid, the bottle lid runs through and is provided with crushing frame, the bottom of smashing the frame is circular dull and stereotyped and is provided with a plurality of aperture bodies, circular dull and stereotyped and crushing bottle interior bottle footpath unanimity.
In the present invention, preferably, the crushing blade is a cylindrical serrated blade.
In the utility model, preferably, a manual stirrer is arranged in the reaction extraction bottle, the manual stirrer is connected with the reaction extraction bottle in a sealing and rotating way, a rotating rod is arranged on the manual stirrer, and the rotating rod is arranged outside the reaction extraction bottle; the side wall of the reaction extraction bottle is provided with a sample adding hole; the smooth undergauge in bottom of bottle is drawed in the reaction and is provided with the control valve, be provided with on the outer wall of reaction draw bottle bottom liquid outlet and filter mounting assorted screw thread.
The utility model discloses in, it is preferred, the lateral wall bottom of placing the bottle is provided with the liquid outlet.
The utility model discloses in, it is preferred, it is the cavity ring to filter the mounting, the both ends of filtering the mounting are provided with respectively and place the inlet of bottle and draw the respective assorted internal thread of bottle liquid outlet with the reaction, it is provided with semi-annular opening on the mounting to filter, be equipped with semicircular draw-in groove on the inner wall of filtering the mounting, it is fixed with the draw-in groove joint that the filter screen runs through semi-annular opening, threaded connection has fixed cover on the outer wall of the intermediate position of filtering the mounting.
The utility model discloses in, preferred, the material that crushing bottle, reaction extracted the bottle, placed the bottle, smashed the frame is polytetrafluoroethylene.
The utility model discloses in, preferred, whole extraction element places on the experiment frame, the crushing bottle of the fixed centre gripping of clamping device of experiment frame.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model is provided with the crushing bottle, the reaction extraction bottle and the placing bottle which are mutually connected in a sealing way, and the crushing, extraction and transfer processes can be prevented from contacting with air, so the risk of interference by bacteria is greatly avoided; simultaneously, this device has reduced manual operation's step, and the reaction is drawed the bottle and is placed and be provided with the filtration mounting between the bottle, can further filter the extract for place the extract that the bottle was stayed at last and keep pure.
Drawings
FIG. 1 is a schematic view of the overall structure of an umbilical cord stem cell extraction device of the present invention;
FIG. 2 is a schematic structural diagram of a crushing frame of the umbilical cord stem cell extraction device of the present invention;
FIG. 3 is a schematic structural diagram of a bottle cap of the umbilical cord stem cell extraction device of the present invention;
FIG. 4 is a cross-sectional view of a filter fixture of an umbilical cord stem cell extraction device of the present invention;
FIG. 5 is a schematic view of the connection between the filter fixing member and the filter screen of the device for extracting umbilical cord stem cells of the present invention;
FIG. 6 is a schematic structural diagram of a hand stirrer of the umbilical cord stem cell extraction device of the present invention.
Description of the main elements in the figures: 1-crushing the bottle; 2-reaction extraction bottle; 3, placing a bottle; 4-a filter fixture; 5, filtering the filter screen; 6-bottle body; 7-bottle cap; 8-opening and closing the door; 9-crushing cutter; 10-crushing frame; 11-a small pore body; 12-a manual stirrer; 13-a sample addition port; 14-a control valve; 15-a liquid outlet; 16-a fixation sleeve; 17-experimental frame; 18-a clamping device; 19-a card slot; 20-rotating rods; 21-a through hole; 22-fixed column.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 to 6, the utility model provides an umbilical cord stem cell extraction device, which comprises a crushing bottle 1, a reaction extraction bottle 2 and a placing bottle 3, wherein the crushing bottle 1 and the reaction extraction bottle 2 are communicated with each other and integrally formed from top to bottom, and the placing bottle 3 is arranged at the lower side of the reaction extraction bottle 2; the placing bottle 3 is connected with the reaction extraction bottle 2 through a filtering fixing piece 4; a double-layer filter screen 5 is arranged in the filter fixing part 4; the bottleneck department that the bottle 2 was drawed in the reaction and place bottle 3 all is provided with the screw thread, the bottle 2 was drawed in the reaction and place bottle 3 with filter 4 threaded fixed connection. The placenta is smashed into the fritter in smashing bottle 1, then gets into to react and draws in reaction extraction bottle 2, then enters into through filter screen 5 in filtering mounting 4 and places and deposit in bottle 3, and whole journey reacts and shifts in sealed device, does not contact air and other devices, avoids receiving the pollution. Filter screen 5 plays filterable effect when the extract enters into placing bottle 2 by reaction extraction bottle 1, just keeps off in filter screen 5 top if there is big complete fragment of unreacted, sets up double-deck filter screen 5 and can make filterable more meticulous accuracy.
In this embodiment, the crushing bottle 1 comprises a bottle body 6 and a bottle cap 7 in threaded connection with the bottle body 6, the bottle body 6 is provided with an opening and closing door 8, and before crushing, the opening and closing door 8 can be opened to place the placenta into the crushing frame 10 through the opening and closing door 8. As shown in fig. 2 and 3, a fixing column 22 is fixedly arranged on the bottom wall of the bottle cap 7, and a crushing knife 9 is fixedly connected to the bottom of the fixing column 22 through threads; the bottle cap 7 is provided with a through hole 21, the bottle cap 7 penetrates through the through hole 21 and is provided with a crushing frame 10, the bottom of the crushing frame 10 is a circular flat plate and is provided with a plurality of small hole bodies 11, and the circular flat plate is consistent with the inner diameter of the crushing bottle 1.
In this embodiment, the crushing blade 9 is a cylindrical serrated blade, and when the placenta is crushed to a certain size by the crushing blade 9, the placenta can fall down from the small hole body 11 and enter the reaction extraction flask 2.
In the present embodiment, a manual stirrer 12 is arranged in the reaction extraction bottle 2, the manual stirrer 12 is connected with the reaction extraction bottle 2 in a sealing and rotating manner, a rotating rod 20 is arranged on the manual stirrer 12, and the rotating rod 20 is arranged outside the reaction extraction bottle 2; the side wall of the reaction extraction bottle 2 is provided with a sample adding hole 13; the smooth undergauge in bottom of reaction extraction bottle 2 just is provided with control valve 14, be provided with on the outer wall of reaction extraction bottle 2 bottom liquid outlet and filter mounting 4 assorted screw thread. Rotating the rotating rod 20 can rotate the hand stirrer 12 to stir the substance in the reaction extraction flask 2, and the filling hole 13 is filled with a stopper when it is not needed, and when it is needed to add the enzyme preparation into the reaction extraction flask 2, the stopper needs to be taken out, and the enzyme preparation is added into the reaction extraction flask 2 through the filling hole 13.
In this embodiment, the bottom of the side wall of the placing bottle 3 is provided with a liquid outlet 15, and the liquid outlet 15 can conveniently discharge the filtered extracting solution in the placing bottle 3 for use.
In this embodiment, filter mounting 4 is the cavity ring, the both ends of filtering mounting 4 are provided with respectively and place the inlet of bottle 3 and draw out 2 liquid mouths assorted internal threads separately with the reaction with the bottle, be provided with the semi-annular opening on the filtering mounting 4, be equipped with semicircular draw-in groove 19 on the inner wall of filtering mounting 4, semi-annular opening and draw-in groove 19 are in same horizontal plane, filter screen 5 runs through the semi-annular opening and is fixed with draw-in groove 19 joint, threaded connection has fixed cover 16 on the outer wall of the intermediate position of filtering mounting 4. The fixing sleeve 16 is used for fixing the filter screen 5 inside the filter fixing part 4, and plays a role in sealing to prevent bacteria from corroding in the filtering process.
Filter mounting 4 and be the cavity ring, the both ends of filtering mounting 4 are provided with respectively and place the inlet of bottle 3 and draw the bottle separately assorted internal thread of 2 liquid mouths with the reaction, be equipped with circular snap ring 19 on the inner wall of 4 intermediate positions of filtering mounting, the wherein semi-ring of circular snap ring 19 runs through and filters mounting 4, the card dress is fixed with filter screen 5 in the circular snap ring 19, threaded connection has fixed cover 16 on the outer wall of the intermediate position of filtering mounting 4. The fixing sleeve 16 is used for fixing
In the present embodiment, the material of the pulverizing bottle 1, the reaction extraction bottle 2, the placing bottle 3, and the pulverizing frame 10 is polytetrafluoroethylene. The cleaning agent is made of polytetrafluoroethylene materials, is convenient to clean, and cannot react with additives chemically.
In this embodiment, the entire extraction device is placed on a laboratory bench 17, and a holding device 18 of the laboratory bench 17 holds the crushing bottle in a fixed manner. The experimental frame 17 can be used as an auxiliary device to fix the extraction device, so that the extraction device can be kept stable in the using process.
When the extraction device is used, firstly, the whole device is assembled according to the structure and is placed on an experiment frame for fixing; secondly, placing the placenta on a circular flat plate at the bottom of a crushing frame 10 in a crushing bottle through an opening-closing door 8, closing the opening-closing door 10, manually drawing the crushing frame 10, crushing the placenta into small round blocks under the matching of a crushing knife 9 and the crushing frame 10, and enabling the small round blocks to fall into a reaction extraction bottle 2 through the bottom of the crushing bottle 1 after being crushed; thirdly, adding the required enzyme preparation or other reaction extract into the reaction extraction bottle 2 through the sample adding hole 13, and if the reaction requires stirring, manually rotating the rotating rod 20 to stir; after the reaction draws a period of time, after the reaction is accomplished, open control valve 14, after the extract carries out twice filtration via filtering mounting 4, the pure extract enters into places bottle 3 and deposits, when needs, can open liquid outlet 15 and emit the extract and use, can will place bottle 3 simultaneously and from filtering mounting 4 unscrewing, will place bottle 3 sealed place can.
After the use is accomplished, can unscrew bottle lid 7, its control valve 14 is opened, and filter mounting 4 is rotatory to be lifted off, can conveniently wash and disinfect crushing bottle 1, reaction extraction bottle 2, filter mounting 4, bottle lid 7 and crushing frame 10 this moment, and it is convenient to wash, is convenient for later continue to use.
The above description is for the detailed description of the preferred possible embodiments of the present invention, but the embodiments are not intended to limit the scope of the present invention, and all equivalent changes or modifications accomplished under the technical spirit suggested by the present invention should fall within the scope of the present invention.
Claims (8)
1. The umbilical cord stem cell extraction device is characterized by comprising a crushing bottle (1), a reaction extraction bottle (2) and a placing bottle (3), wherein the crushing bottle (1) and the reaction extraction bottle (2) are communicated with each other and integrally formed from top to bottom, and the placing bottle (3) is arranged on the lower side of the reaction extraction bottle (2); the placing bottle (3) is connected with the reaction extraction bottle (2) through a filtering fixing piece (4); a double-layer filter screen (5) is arranged in the filter fixing piece (4); the reaction extracting bottle (2) and the bottle mouth of the placing bottle (3) are both provided with threads, and the reaction extracting bottle (2) and the placing bottle (3) are fixedly connected with the filtering fixing piece (4) through threads.
2. The extraction device for the umbilical cord stem cells as claimed in claim 1, wherein the crushing bottle (1) comprises a bottle body (6) and a bottle cap (7) in threaded connection with the bottle body (6), the bottle body (6) is provided with an opening and closing door (8), the bottom wall of the bottle cap (7) is fixedly provided with a crushing knife (9), the bottle cap (7) is provided with a crushing frame (10) in a penetrating manner, the bottom of the crushing frame (10) is a circular flat plate and is provided with a plurality of small hole bodies (11), and the diameter of the circular flat plate is consistent with that of the inner diameter of the crushing bottle (1).
3. The umbilical cord stem cell extraction device of claim 2, wherein the crushing knife (9) is a cylindrical serrated knife.
4. The extraction device for the umbilical cord stem cells as claimed in claim 1, wherein a manual stirrer (12) is arranged in the reaction extraction bottle (2), the manual stirrer (12) is connected with the reaction extraction bottle (2) in a sealing and rotating manner, a rotating rod (20) is arranged on the manual stirrer (12), and the rotating rod (20) is arranged outside the reaction extraction bottle (2); the side wall of the reaction extraction bottle (2) is provided with a sample adding hole (13); the bottom of the reaction extraction bottle (2) is smooth in diameter shrinkage and is provided with a control valve (14), and threads matched with the filtering fixing piece (4) are arranged on the outer wall of a liquid outlet at the bottom of the reaction extraction bottle (2).
5. The umbilical cord stem cell extraction device of claim 1, wherein the side wall bottom of the placing bottle (3) is provided with a liquid outlet (15).
6. The extraction device for the stem cells of the umbilical cord as claimed in claim 1, wherein the filtering fixture (4) is a hollow circular ring, the two ends of the filtering fixture (4) are respectively provided with an internal thread matching with the liquid inlet of the placing bottle (3) and the liquid outlet of the reaction extracting bottle (2), the filtering fixture (4) is provided with a semi-circular opening, the inner wall of the filtering fixture (4) is provided with a semi-circular clamping groove (19), the filter screen (5) penetrates through the semi-circular opening and is clamped and fixed with the clamping groove (19), and the outer wall of the middle position of the filtering fixture (4) is connected with a fixing sleeve (16) in a threaded manner.
7. The extraction device for the umbilical cord stem cells as claimed in claim 1, wherein the materials of the crushing bottle (1), the reaction extraction bottle (2), the placing bottle (3) and the crushing frame (10) are all polytetrafluoroethylene.
8. The umbilical cord stem cell extraction device of claim 1, wherein the whole extraction device is placed on a test rack (17), and the clamping device (18) of the test rack (17) fixedly clamps the crushing bottle (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821836552.6U CN210085443U (en) | 2018-11-08 | 2018-11-08 | Umbilical cord stem cell extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821836552.6U CN210085443U (en) | 2018-11-08 | 2018-11-08 | Umbilical cord stem cell extraction element |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210085443U true CN210085443U (en) | 2020-02-18 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821836552.6U Active CN210085443U (en) | 2018-11-08 | 2018-11-08 | Umbilical cord stem cell extraction element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210085443U (en) |
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2018
- 2018-11-08 CN CN201821836552.6U patent/CN210085443U/en active Active
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