CN210030711U - Automatic cell filter equipment of stirring - Google Patents

Abstract

The utility model belongs to the technical field of cell filtration devices, in particular to an automatic stirring cell filtration device, comprising a filter, an observation window opened on the front end of the filter, and a cell filter screen fixed inside the filter. It is a cylindrical structure, the inner center of the filter is provided with a vertical stirring shaft, the stirring shaft penetrates the cell filter screen, and a plurality of groups of equally spaced stirring rods are fixed on the outside of the stirring shaft. It is located above the cell strainer, a group of material-removing rods are arranged under the stirring rod, the material-removing rod is fixedly connected with the stirring shaft, and a material-removing brush is fixed under the material-removing rod, and the bottom end of the material-removing brush is In contact with the upper surface of the cell filter, the top of the stirring shaft extends to the top of the filter, which overcomes the deficiencies of the prior art, prevents the microcarriers from clogging the screen, improves the efficiency of cell filtration, and facilitates the repeated use of the screen. Utilize it, reduce the cost of use, and reduce the waste of the screen.

Description

An automatic stirring cell filtration device

technical field

The utility model belongs to the technical field of cell filtering devices, in particular to an automatic stirring cell filtering device.

Background technique

Microcarrier culture is currently recognized as the most promising large-scale animal cell culture technology. It combines the advantages of suspension culture and adherent culture, and enables adherent-dependent cells to adhere to the particle surface monolayer during suspension culture. growth, thereby increasing the area of cell attachment growth, which is beneficial to the large-scale culture and collection of cells. The diameter of the microcarrier is 60-200 μm, and the density is generally 1.03-1.05 g/mL. The density can gradually increase with the attachment and growth of cells. At present, the cells are detached from the microcarriers, and trypsin and trypsin substitutes are usually used to digest the cells. After 30-60min of treatment, the microcarriers are detached from the cells to form a free cell and microcarrier mixture. sieve filtration, the microcarriers are trapped on the sieve, and then the cells are collected.

At present, the diameter of the sieve used for cell filtration is only 20-30mm, which is easy to cause the blockage of the sieve. A large number of cells remain on the upper layer of the sieve, which affects the efficiency of cell filtration, and also causes the waste of the sieve and increases the cost. .

Utility model content

The purpose of the utility model is to provide an automatic stirring cell filtration device, which overcomes the deficiencies of the prior art, prevents the microcarriers from clogging the sieve, improves the efficiency of cell filtration, facilitates the multiple utilization of the sieve, and reduces the use cost, Reduced screen waste.

In order to solve the above-mentioned problems, the technical scheme adopted by the present utility model is as follows:

An automatic stirring cell filtration device, comprising a filter, an observation window opened on the front end of the filter, and a cell filter screen fixed inside the filter, the filter is a cylindrical structure, and the inner center of the filter is A vertical stirring shaft is provided, the stirring shaft runs through the cell strainer, and a plurality of groups of equally spaced stirring rods are fixed on the outside of the stirring shaft. The stirring rods are located above the cell strainer, and the stirring rods are arranged below There is a set of feeding rods, the feeding rods are fixedly connected with the stirring shaft, a feeding brush is fixed under the feeding rod, the bottom end of the feeding brush is in contact with the upper surface of the cell filter, and the stirring The top of the shaft extends to the top of the filter, a driven gear is fixed on the top of the stirring shaft, a rotating motor is fixed on the top of the filter, and a driving gear is fixed on the top of the output shaft of the rotating motor. , the driving gear meshes with the driven gear;

The top side of the filter is connected with a liquid inlet pipe, the bottom end of one side of the filter is connected with a liquid outlet pipe, and a slag discharge port is opened on the outer wall of one side of the filter. The bottom end is at the same level as the upper surface of the cell strainer.

Further, a horizontal support seat is arranged below the filter, a support rod and a hydraulic cylinder are arranged between the support seat and the lower end surface of the filter, and the support rod and the hydraulic cylinder are about the longitudinal centerline of the filter Symmetrically, the bottom end of the support rod is fixedly connected with the support seat, the top end of the support rod is hinged on the lower end surface of the filter, and the two ends of the hydraulic cylinder are hinged with the support seat and the filter respectively.

Further, the front end of the filter is provided with a control panel, and the output end of the control panel is electrically connected to the input end of the rotating motor and the input end of the hydraulic cylinder, respectively.

Further, a fixing plate is fixed at one end of the stirring rod away from the stirring shaft, and a cleaning brush is fixed on the side of the fixing plate away from the stirring rod, and the cleaning brush is in contact with the inner wall of the filter.

Further, a transmission box is fixed above the filter, the top end of the stirring shaft is rotatably connected with the inner wall of the top end of the transmission box, and the rotating motor is located in the transmission box.

Further, the diameter of the driven gear is three times that of the driven gear.

Further, a sealing cover is threadedly connected to the outside of the slag discharge port, and a control valve is provided on the liquid outlet pipe.

Compared with the prior art, the utility model has the following beneficial effects:

The automatic stirring cell filtering device of the utility model is provided with a filter, a transmission box, a liquid inlet pipe, a slag discharge port, a liquid outlet pipe, a cell filter screen, a cleaning brush, a stirring rod, a rotating motor, a driving gear, The driven gear, stirring shaft, fixed plate, feeding rod and feeding brush are driven by the rotating motor to slowly rotate the stirring shaft, the stirring rod stirs the filter, the cleaning brush cleans the residual liquid on the inner wall, and the feeding rod and dial The material brush cleans the residue on the cell filter to prevent clogging; by setting the support seat, hydraulic cylinder, support rod, and hydraulic cylinder to lift the filter, the filter is inclined, and the slag discharge and liquid discharge of the filter are accelerated.

The utility model is simple in structure and convenient to use, avoids microcarriers from clogging the sieve, improves the efficiency of cell filtration, facilitates the multiple utilization of the sieve, reduces the use cost, reduces the waste of the sieve, and has good economic value.

Description of drawings

FIG. 1 is a front view of an automatic stirring cell filter device.

FIG. 2 is a schematic structural diagram of an automatic stirring cell filtration device.

FIG. 3 is a block diagram of the electrical control principle of an automatic stirring cell filtration device.

In the picture: 1. Filter; 2. Gearbox; 3. Observation window; 4. Liquid inlet pipe; 5. Slag discharge port; 6. Liquid outlet pipe; 7. Control panel; 8. Cell strainer; 9. Cleaning Brush; 10, stirring rod; 11, rotating motor; 12, driving gear; 13, driven gear; 14, stirring shaft; 15, fixing plate; 16, feeding rod; 17, feeding brush; 18, supporting rod; 19. Support seat; 20. Hydraulic cylinder.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the implementations. example. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

As shown in Figures 1-3, the automatic stirring cell filter device of the present invention comprises a filter 1, an observation window 3 opened on the front end of the filter 1 and a cell filter screen fixed inside the filter 1 8, the filter 1 is a cylindrical structure, the inner center of the filter 1 is provided with a vertical stirring shaft 14, the stirring shaft 14 runs through the cell strainer 8, and the outside of the stirring shaft 14 is fixed with multiple groups of equally spaced stirring rods 10, The stirring rods 10 are all located above the cell strainer 8, and a group of feeding rods 16 are arranged below the stirring rod 10. The feeding rods 16 are fixedly connected with the stirring shaft 14, and a feeding brush 17 is fixed below the feeding rod 16. The feeding brushes The bottom end of 17 is in contact with the upper surface of the cell strainer 8, the top of the stirring shaft 14 extends to the top of the filter 1, the outside of the top of the stirring shaft 14 is fixed with a driven gear 13, and the top of the filter 1 is fixed with a rotating gear 13. The motor 11, the model of the rotating motor 11 can be DRF-W300CA, a driving gear 12 is fixed on the outer side of the top end of the output shaft of the rotating motor 11, and the driving gear 12 is meshed with the driven gear 13;

The top side of the filter 1 is connected with a liquid inlet pipe 4, the bottom end of one side of the filter 1 is connected with a liquid outlet pipe 6, and a slag discharge port 5 is opened on the outer wall of one side of the filter 1, and the bottom of the slag discharge port 5 is provided. The ends are at the same level as the upper surface of the cell strainer 8 .

A horizontal support seat 19 is arranged below the filter 1, and a support rod 18 and a hydraulic cylinder 20 are arranged between the support seat 19 and the lower end face of the filter 1. The hydraulic cylinder 20 can be ROC32*16*50, The support rod 18 and the hydraulic cylinder 20 are symmetrical with respect to the longitudinal centerline of the filter 1. The bottom end of the support rod 18 is fixedly connected with the support base 19, and the top end of the support rod 18 is hinged on the lower end surface of the filter 1. The ends are hinged with the support base 19 and the filter 1 respectively; the front end of the filter 1 is provided with a control panel 7, the model of the control panel 7 can be GKB10UIR, and the output end of the control panel 7 is respectively connected with the input end of the rotating motor 11 and the hydraulic pressure The input end of the cylinder 20 is electrically connected; the end of the stirring rod 10 away from the stirring shaft 14 is fixed with a fixed plate 15 , and the side of the fixed plate 15 away from the stirring rod 10 is fixed with a cleaning brush 9 , and the cleaning brush 9 is connected to the inner wall of the filter 1 The top of the filter 1 is fixed with a transmission box 2, the top of the stirring shaft 14 is rotatably connected to the inner wall of the top end of the transmission box 2, and the rotating motor 11 is located in the transmission box 2; the diameter of the driven gear 13 is three times the diameter of the driven gear 13. The outer side of the slag discharge port 5 is threadedly connected with a sealing cover, and the liquid outlet pipe 6 is provided with a control valve.

To sum up, the automatic stirring cell filter device of the present invention, when in use, the cell mixture to be filtered is added to the filter 1 through the liquid inlet pipe 4, and the microcarriers in the cell mixture remain in the cell filter. 8, the cells are discharged from the liquid outlet pipe 6 downward through the cell filter 8; while filtering, the rotating motor 11 is started, and the rotating motor 11 drives the stirring shaft 14 to rotate slowly through the driving gear 12 and the driven gear 13, and the stirring rod 10 Stir the cell mixture in the filter 1, the cleaning brush 9 brushes on the inner wall of the filter 1, and cleans the residual liquid on the inner wall of the filter 1, and the feeding rod 16 rotates with the stirring shaft 14 to drive the feeding brush. 17 Swipe on the cell strainer 8 to clean up the residue of the microcarriers on the cell strainer 8 to avoid clogging; after completing the filtration, start the hydraulic cylinder 20 to extend the hydraulic cylinder 20, and the filter 1 is directed toward the liquid outlet pipe 6. One side is inclined to speed up the liquid discharge and slag discharge, and it is convenient to use.

It will be apparent to those skilled in the art that the present invention is not limited to the details of the above-described exemplary embodiments, and that the present invention may be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments are to be considered in all respects as exemplary and not restrictive, and the scope of the present invention is defined by the appended claims rather than the foregoing description, and it is therefore intended that the All changes within the meaning and range of the required equivalents are embraced within the present invention. Any reference signs in the claims shall not be construed as limiting the involved claim.

Claims (7)

1. A cell filtration device for automatic stirring, comprising a filter (1), an observation window (3) provided on the front end of the filter (1) and a cell filter screen (8) fixed inside the filter (1) , characterized in that: the filter (1) is a cylindrical structure, the inner center of the filter (1) is provided with a vertical stirring shaft (14), and the stirring shaft (14) penetrates the cell filter (14). 8), a plurality of groups of equally spaced stirring rods (10) are fixed on the outside of the stirring shaft (14), and the stirring rods (10) are all located above the cell strainer (8) and below the stirring rod (10). A set of material-removing rods (16) are provided, the material-removing rods (16) are fixedly connected with the stirring shaft (14), and a material-removing brush (17) is fixed under the material-removing rod (16), and the material-removing rods (16) are The bottom end of the brush (17) is in contact with the upper surface of the cell strainer (8), the top of the stirring shaft (14) extends to the top of the filter (1), and the top of the stirring shaft (14) is fixed on the outside There is a driven gear (13), a rotating motor (11) is fixed on the top of the filter (1), and a driving gear (12) is fixed on the outside of the top of the output shaft of the rotating motor (11). The gear (12) meshes with the driven gear (13); A liquid inlet pipe (4) is connected to the top side of the filter (1), a liquid outlet pipe (6) is connected to the bottom end of one side of the filter (1). A slag discharge port (5) is provided on the side outer wall, and the bottom end of the slag discharge port (5) is on the same horizontal plane as the upper surface of the cell filter screen (8). 2. The automatic stirring cell filtration device according to claim 1, characterized in that: a support seat (19) in a horizontal direction is provided below the filter (1), and the support seat (19) is arranged with the support seat (19). A support rod (18) and a hydraulic cylinder (20) are arranged between the lower end faces of the filter (1), and the support rod (18) and the hydraulic cylinder (20) are symmetrical with respect to the longitudinal centerline of the filter (1), so The bottom end of the support rod (18) is fixedly connected with the support seat (19), the top end of the support rod (18) is hinged on the lower end surface of the filter (1), and the two ends of the hydraulic cylinder (20) are respectively Hinged with the support base (19) and the filter (1). 3. The cell filtration device of a kind of automatic stirring according to claim 1 or 2, characterized in that: the front surface of the filter (1) is provided with a control panel (7), and the The output ends are respectively electrically connected with the input end of the rotating motor (11) and the input end of the hydraulic cylinder (20). 4. The cell filtration device for automatic stirring according to claim 1, characterized in that: one end of the stirring rod (10) away from the stirring shaft (14) is fixed with a fixing plate (15), and the fixing plate (15) A cleaning brush (9) is fixed on the side away from the stirring rod (10), and the cleaning brush (9) is in contact with the inner wall of the filter (1). 5. The cell filtration device for automatic stirring according to claim 1, characterized in that: a transmission box (2) is fixed above the filter (1), and the top of the stirring shaft (14) is connected to the The inner wall of the top end of the transmission case (2) is rotatably connected, and the rotating motor (11) is located in the transmission case (2). 6 . The automatic stirring cell filtration device according to claim 4 , wherein the diameter of the driven gear ( 13 ) is three times that of the driven gear ( 13 ). 7 . 7 . The automatic stirring cell filtration device according to claim 4 , wherein a sealing cover is threadedly connected to the outside of the slag discharge port ( 5 ), and a control valve is provided on the liquid outlet pipe ( 6 ). 8 . .

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