CN209974786U - Liquid aspirator for cell culture experiments - Google Patents

Abstract

The utility model relates to a liquid aspirator for cell culture experiments, which comprises a waste liquid tank and an air suction pump. The top of the waste liquid tank is provided with an air suction port and a liquid inlet port. One end of the hose is connected, the other end of the hose is connected with the elbow suction pipe, and the elbow suction pipe is a rigid pipe or a hard pipe; a controller and a button are arranged on the outside of the waste liquid tank, and the signal output end of the button is connected with the signal input end of the controller; The controlled end of the air pump is connected with the control end of the controller. The utility model has the advantages of simple structure and convenient use, and can continuously suck out the cell culture liquid.

Description

Liquid aspirator for cell culture experiments

technical field

The utility model relates to a liquid aspirator for cell culture experiments, which belongs to the technical field of biological experimental instruments.

Background technique

As far as the inventors know, a cell culture medium replacement operation is often required in cell culture experiments, that is, the culture medium in the cell culture container is sucked off first, and then fresh culture medium is transferred into the container. The inventors found in practice that, when aspirating the culture medium, it is necessary to use a pipetting device or a dropper to aspirate the liquid from the container again and again, and then discharge it into the waste liquid cup, which wastes time and energy. For this kind of operation that does not require accurate measurement of liquid volume, if there is a device dedicated to continuously suctioning the liquid, the efficiency will be significantly improved.

After searching, it is found that the Chinese utility model patent with the application number CN201620011977.8 and the authorized announcement number CN205340843U discloses an electric pipette, including a pipette, a gas flow meter, a gas pumping or hydraulic pressure device and a control system; a gas flow meter The air inlet is connected to the upper end of the pipette through an electric valve, and the air outlet is connected to an air extraction or hydraulic pressure device; the control system is respectively connected to a gas flow meter, an electric valve, and an air extraction or hydraulic pressure device. The device can automatically and accurately pipette larger volumes of liquid. However, this technical solution cannot realize the operation of continuously aspirating the cell culture medium.

The Chinese utility model patent with application number CN201720263068.8 and authorization announcement number CN207287472U discloses a continuous pipette, including a standard pipetting handle and a standard pipetting tip matched with it, and the standard pipetting handle is matched with a collection container through a connecting tube The collection container is connected with the air extraction pump through the air extraction pipe; the bottle mouth of the collection container is matched with a sealing plug, the air extraction pipe is provided with a filter plug, the connecting pipe is a hose, and the air extraction pump is a roller type negative pressure air extraction pump. Although this technical solution can realize the continuous suction and removal of the liquid in the container, the standard pipetting handle needs to be modified so that it can communicate with the connecting pipe, which is still not simple enough.

Utility model content

The purpose of the utility model is to: in view of the problems existing in the above-mentioned prior art, to propose a liquid aspirator for cell culture experiments, which has a simple structure, is convenient to use, and can continuously absorb the cell culture liquid.

In order to achieve the above purpose, the technical scheme of the present utility model is as follows:

The liquid aspirator for cell culture experiments includes a waste liquid tank and an air suction pump. It is characterized in that the top of the waste liquid tank has an air suction port and a liquid inlet port. The mouth is communicated with one end of the hose, and the other end of the hose is communicated with the elbow suction pipe, and the elbow suction pipe is a rigid pipe or a hard pipe; a controller and a button are arranged on the outside of the waste liquid tank, and the button is arranged on the outside of the waste liquid tank. The signal output end is connected with the signal input end of the controller; the controlled end of the air pump is connected with the control end of the controller.

In this structure, there can be multiple elbow straws, so that the sterilized elbow straws can be replaced according to the situation during operation to ensure aseptic operation and avoid cross-contamination, and the elbow straws can be directly used with existing products, which is conducive to reducing Cost; the elbow suction pipe is connected with the hose, which can maintain a good degree of activity, which is convenient for operation; the start and stop of the air pump can be controlled by the button, which is easy to operate. In this way, the cell culture medium can be continuously aspirated through a simple structure, which is beneficial to improve work efficiency.

The utility model can also adopt the following preferred solutions:

Preferably, the buttons include a start button and a shutdown button. The liquid aspirator has: an external force triggers a start button to enable the controller to turn on the air pump, thereby keeping the elbow suction pipe in a suction state of suction; the liquid aspirator also has: an external force triggers a close button to make the controller turn off the air pump shutdown state.

By adopting this preferred solution, the start-stop control structure of the air pump can be made simpler.

Preferably, a liquid level upper limit sensor is provided inside the waste liquid tank, and the signal output end of the liquid level upper limit sensor is connected with the signal input end of the controller. The liquid suction remover also has a protection state in which the liquid level in the waste liquid tank triggers a liquid level upper limit sensor to make the controller close the air pump.

With this preferred solution, the liquid can be prevented from being sucked into the air pump by mistake, and the pump body can be prevented from being damaged.

Preferably, the waste liquid tank has a tank body and a top cover, and the top cover is sealedly connected to the tank body; the air suction port and the liquid inlet port are respectively provided on the top cover.

More preferably, the tank body of the waste liquid tank has an inverted conical bottom, the bottom end of the bottom has a drainage channel, and the drainage channel has a liquid outlet located on the side of the tank body, and the outlet is located at the side of the tank body. The liquid port is communicated with the liquid discharge pipe; the liquid discharge channel has a solenoid valve, and the controlled end of the solenoid valve is connected with the control end of the controller; the button includes a liquid discharge button. The liquid aspirator has: external force triggers the liquid discharge button to make the controller close the suction pump and open the solenoid valve, so that the liquid in the waste liquid tank is discharged to the outside of the waste liquid tank through the liquid discharge channel, the liquid outlet and the liquid discharge pipe in turn. the discharge state.

With this preferred solution, the liquid in the waste liquid tank can be further discharged to the outside (such as external liquid collecting tank, etc.) as required, which is suitable for the experimental scenario of removing cell culture liquid in large quantities; Drain fluid.

More preferably, the air suction port and the liquid inlet are respectively provided with air nozzles, and the air nozzle is located outside the waste liquid tank; the air nozzle of the air suction port is connected to the pipeline, and the air nozzle of the liquid inlet is connected to the soft pipe connection; the top cover has internal threads, the top of the tank body has external threads, the top cover is connected with the top of the tank body by threads; a sealing ring is arranged in the top cover.

Preferably, the controller is connected to a battery or an external power source; the air pump is connected to an external power source; and the material of the elbow pipette is glass material.

The utility model has the advantages of simple structure and convenient use, and can continuously suck out the cell culture liquid, which is beneficial to improve the work efficiency.

Description of drawings

The utility model will be further described below in conjunction with the accompanying drawings.

FIG. 1 is a schematic structural diagram of an embodiment of the present invention.

FIG. 2 is a schematic structural diagram of a waste liquid tank according to an embodiment of the present invention.

FIG. 3 is an electrical schematic diagram of an embodiment of the utility model.

Detailed ways

Example

As shown in Figures 1 to 3, the liquid aspirator for cell culture experiments in this embodiment includes a waste liquid tank 01 and an air pump 02. The top of the waste liquid tank 01 has a suction port 03 and a liquid inlet 04, and the suction port 03 It is communicated with the air pump 02 through the pipeline 05, the liquid inlet 04 is communicated with one end of the hose 06 (such as a rubber hose, etc.), and the other end of the hose 06 is communicated with the elbow suction pipe 07, and the elbow suction pipe 07 is a rigid pipe or a hard pipe ( Such as glass tubes, metal tubes, etc.); a controller and a button 08 are arranged on the outside of the waste liquid tank 01, and the signal output end of the button 08 is connected with the signal input end of the controller; the controlled end of the air pump 02 is connected with the control end of the controller connect. Buttons 08 include a start button, a shutdown button, and a drain button.

A liquid level upper limit sensor 09 is arranged inside the waste liquid tank 01, and the signal output end of the liquid level upper limit sensor 09 is connected with the signal input end of the controller.

Specifically, the waste liquid tank 01 has a tank body 10 and a top cover 11 , and the top cover 11 is sealedly connected to the tank body 10 ; The tank body 10 of the waste liquid tank 01 has an inverted conical bottom 12, the bottom end of the bottom 12 has a drainage channel 13, and the drainage channel 13 has a liquid outlet 14 located on the side of the tank body 10, and the liquid outlet 14 It communicates with the drain pipe 15; the drain channel 13 has a solenoid valve 16, and the controlled end of the solenoid valve 16 is connected to the control end of the controller.

The air suction port 03 and the liquid inlet 04 have air nozzles respectively, and the air nozzles are located outside the waste liquid tank 01 ;

The top cover 11 has internal threads, the top of the can body 10 has external threads, and the top cover 11 is threadedly connected to the top of the can body 10 . The top cover 11 is provided with a sealing ring.

In addition, the controller is connected to a battery or an external power source (not shown). The air pump 02 is connected to an external power source (not shown in the figure). In this embodiment, the material of the elbow straw 07 is glass material.

The liquid aspirator of this embodiment has the following specific states:

(1) The external force triggers the start button to enable the controller to turn on the air pump 02, thereby keeping the elbow suction pipe 07 in the suction state of suction.

(2) External force triggers the shutdown button to make the controller close the shutdown state of the air pump 02 .

(3) The liquid level in the waste liquid tank 01 triggers the liquid level upper limit sensor 09 to make the controller close the protection state of the air pump 02 .

(4) The external force triggers the liquid discharge button to make the controller close the air pump 02 and open the solenoid valve 16, so that the liquid in the waste liquid tank 01 is sequentially discharged to the waste liquid tank through the liquid discharge channel 13, the liquid outlet 14 and the liquid discharge pipe 15. Drainage status outside 01.

The use process of this embodiment is:

The parts of the liquid aspirator are sterilized in an appropriate manner, placed in the ultra-clean bench, and assembled. The drain pipe 15 is partly located in the ultra-clean bench and partly outside the ultra-clean bench, which is convenient for draining the liquid to the in an external collection container. Press the start button to make the liquid aspirator in the suction state, hold the elbow pipette, extend into the cell culture container (such as cell culture flask, cell culture dish, etc.) Replace the elbow pipette to avoid cross-contamination. After the operation is completed, press the close button to make the liquid aspirator stop. During the operation, if there is too much liquid in the waste liquid tank 01 and the liquid level upper limit sensor 09 is triggered, the liquid aspirator enters the protection state and stops suctioning the liquid. In the protection state, or when necessary, press the drain button to make the liquid aspirator in the drain state, and the liquid in the waste liquid tank 01 can be drained to the external collection container.

In addition to the above-mentioned embodiments, the present invention may also have other implementations. All technical solutions formed by equivalent replacement or equivalent transformation fall within the protection scope required by the present invention.

It should be noted that the electrical components in this embodiment are all composed of existing electronic components. If this embodiment involves software control programs in the implementation process, the involved software control programs are all mature prior art products. At the same time, this embodiment only involves the use of existing software control programs, and there is no software control program. Improvements to the control program itself.

Claims (10)

1. the liquid aspirator for cell culture experiments comprises a waste liquid tank and an air pump, and it is characterized in that, the top of the waste liquid tank has a suction port and a liquid inlet, and the air suction port is communicated with the suction pump through a pipeline, and the described The liquid inlet is communicated with one end of the hose, and the other end of the hose is communicated with an elbow suction pipe, and the elbow suction pipe is a rigid pipe or a hard pipe; a controller and a button are arranged on the outside of the waste liquid tank, and the The signal output end of the button is connected with the signal input end of the controller; the controlled end of the air pump is connected with the control end of the controller. 2 . The liquid aspirator for cell culture experiments according to claim 1 , wherein the button comprises a start button and a shutdown button. 3 . 3. The liquid aspirator for cell culture experiments according to claim 2, wherein the liquid aspirator has: an external force triggers a start button to make the controller turn on the air pump, and then makes the elbow pipette keep the suction of suction. In addition to the state; the liquid aspirator also has a shutdown state in which the external force triggers the closing button to make the controller close the air pump. 4. The liquid aspirator for cell culture experiments according to claim 1, wherein the inside of the waste liquid tank is provided with a liquid level upper limit sensor, and the signal output end of the liquid level upper limit sensor and the signal of the controller input connection. 5. The liquid aspirator for cell culture experiments according to claim 4, wherein the liquid aspirator also has: the liquid level in the waste liquid tank triggers the liquid level upper limit sensor to make the controller close the protection of the air pump state. 6. The liquid aspirator for cell culture experiments according to claim 1, wherein the waste liquid tank has a tank body and a top cover, and the top cover is sealedly connected with the tank body; The liquid ports are respectively arranged on the top cover. 7. The liquid aspirator for cell culture experiments according to claim 6, wherein the tank body of the waste liquid tank has an inverted conical bottom, and the bottom end of the bottom has a drainage channel, so the The liquid discharge channel has a liquid outlet located on the side of the tank body, and the liquid outlet is communicated with the liquid discharge pipe; the liquid discharge channel has a solenoid valve, and the controlled end of the solenoid valve is connected with the control end of the controller ; The button includes a drain button. 8. The liquid aspirator for cell culture experiments according to claim 7, wherein the liquid aspirator has: an external force triggers a drain button to make the controller close the air pump and open the solenoid valve, thereby making the waste liquid The liquid in the tank is discharged to the liquid discharge state outside the waste liquid tank through the liquid discharge channel, the liquid outlet and the liquid discharge pipe in sequence. 9. The liquid aspirator for cell culture experiments according to claim 6, wherein the air suction port and the liquid inlet port respectively have a gas nozzle, and the gas nozzle is located outside the waste liquid tank; the gas suction port The gas nozzle is connected with the pipeline, the gas nozzle of the liquid inlet is connected with the hose; the top cover has internal threads, the top of the tank body has external threads, and the top cover is threadedly connected to the top of the tank body ; The top cover is provided with a sealing ring. 10. The liquid aspirator for cell culture experiments according to any one of claims 1 to 9, wherein the controller is connected with a battery or an external power source; the air pump is connected with an external power source; The head pipette is made of glass.

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