CN209123436U - A kind of stem cell conditioned medium filter device - Google Patents

Abstract

The utility model discloses a kind of stem cell conditioned medium filter devices, including Rose Box, capping mechanism is rotatably connected on the side wall of the Rose Box, one end of the capping mechanism is flexibly connected with the side wall of Rose Box, the left and right ends of the Rose Box are equipped with through-hole, through equipped with driving mechanism in two through-holes, there are two mobile mechanisms for the interior bottom end rotation connection of the Rose Box, the driving mechanism is meshed with mobile mechanism, filter plate is equipped between two mobile mechanisms, the filter plate is flexibly connected with Liang Ge mobile mechanism respectively.The utility model is used cooperatively by capping mechanism, driving mechanism and mobile mechanism, it can be when replacing filter plate to reach, pass through being used cooperatively for nut and threaded rod, filter plate can be made to move up, it thus can allow user that can more easily replace filter plate, avoid the case where user can not replace filter plate.

Description

A stem cell conditioned medium filter device

technical field

The utility model relates to the technical field of culture medium filtration, in particular to a stem cell conditioned medium filter device.

Background technique

In the process of cell culture, the cells will secrete active substances into the culture medium, among which cytokines are one of the main active substances. After culturing a certain cell or tissue, the culture medium containing cell secretions is called conditioned culture. liquid. Mesenchymal stem cells are non-hematopoietic adult stem cells with multi-directional differentiation and self-renewal capabilities. They are widely present in bone marrow, umbilical cord, umbilical cord blood, peripheral blood, adipose and other tissues. tissues and organs.

Studies have found that mesenchymal stem cells have a paracrine effect (that is, secrete related factors into the culture medium), forming a conditioned medium for stem cells, including stem cell factors, epidermal growth factor, vascular endothelial nerve growth factor, glial-derived neurotrophic factor, etc. , play an important role in the treatment of diseases. In addition to beneficial components such as cytokines, stem cell conditioned medium also contains harmful substances such as wastes from cell growth and metabolism, dead cells and cell debris, and needs to be filtered before use. However, the following problems occur when the existing filter device is used:

When the filter plate is used too many times, the filter plate must be replaced or flushed. If the method of flushing is used, the filter plate may not be cleaned cleanly, but the existing technology is in the process of replacing the filter plate. , because the filter plate is placed inside the filter container, it is inconvenient to take, and it is easy to cause damage to the filter plate in the process of taking it.

Utility model content

The purpose of the utility model is to propose a stem cell conditioned medium filtering device in order to solve the shortcomings in the prior art.

In order to achieve the above-mentioned purpose, the utility model adopts the following technical solutions:

A stem cell conditioned medium filtering device, comprising a filter box, a cover mechanism is rotatably connected to the side wall of the filter box, one end of the cover mechanism is movably connected to the side wall of the filter box, and the left and right sides of the filter box are movably connected. Both ends are provided with through holes, and two through holes are provided with driving mechanisms. The inner bottom end of the filter box is rotatably connected with two moving mechanisms. The driving mechanisms are engaged with the moving mechanisms. A filter plate is arranged between the moving mechanisms, and the filter plates are respectively movably connected with the two moving mechanisms. The lower end of the filter box is provided with a quantitative mechanism, and the quantitative mechanism is connected with the filter box through a connecting pipe.

Preferably, the capping mechanism includes a cap rotatably connected to the side wall of the filter box, an upper end of the cap is provided with a liquid inlet hole, and a cover plate is provided through the liquid inlet hole, and the The lower end is fixedly connected with a clamping column, the lower end of the clamping column is fixedly connected with a first magnetic block, the side wall of the filter box is provided with a groove, and the inner bottom end of the groove is fixedly connected with a second magnetic block, The first magnetic block is movably connected to the second magnetic block.

Preferably, the driving mechanism includes a rotating rod disposed through the through hole, and an end of the rotating rod located in the filter box is fixedly connected with a first helical gear, and the first helical gear is engaged with the moving mechanism.

Preferably, the moving mechanism includes a second helical gear rotatably connected to the inner bottom end of the filter box, the first helical gear meshes with the second helical gear, and a threaded rod is fixedly connected to the upper end of the second helical gear, so The threaded rod is sleeved with a nut, the side wall of the nut is slidably connected with the side wall of the filter box, the side wall of the nut is fixedly connected with an L-shaped support block, and the upper end of the L-shaped support block is fixedly connected with a A spring, the upper end of the spring is fixedly connected with a splint, the splint is slidably connected with the side wall of the L-shaped support block, and the upper and lower ends of the filter plate are respectively movably connected with the splint and the L-shaped support block.

Preferably, a chute is provided on the side wall of the L-shaped support block, and a sliding block is slidably connected with it, the sliding block is connected with the clamping plate, and the clamping plate slides with the sliding groove connect.

Preferably, the quantitative mechanism includes a quantitative box arranged at the lower end of the filter box, the quantitative box is connected with the filter box through a connecting pipe, and two piston plates are slidably connected to the inner side walls of the quantitative box, and the two The upper ends of the piston plates are fixedly connected with baffle plates, the side walls of the two piston plates are fixedly connected with push rods, and one end of the two push rods passes through the side walls of the quantitative box and extends to the outside, so A liquid outlet pipe is fixedly installed at the liquid outlet of the quantitative box, and a one-way valve is fixedly installed on the side wall of the liquid outlet pipe.

Compared with the prior art, the beneficial effects of the present utility model are:

1. Through the cooperation of the capping mechanism, the driving mechanism and the moving mechanism, when the filter plate is replaced, the filter plate can be moved upward through the cooperation of the nut and the threaded rod, so that the user can It is more convenient to replace the filter plate, avoiding the situation that the user cannot replace the filter plate.

2. Through the cooperation of the moving mechanism and the filter plate, the filter plate can be fixed on the L-shaped support plate through the splint, so that the filter plate can no longer be moved.

3. Through the cooperation of the quantitative mechanism, the baffle plate and the piston plate can control the volume of the filtered liquid when the piston plate is moved, so as to achieve the purpose of quantifying the culture liquid.

Description of drawings

Fig. 1 is the structural representation of a kind of stem cell conditioned medium filtering device proposed by the utility model;

Fig. 2 is a partial enlarged schematic diagram at A in Fig. 1;

FIG. 3 is a partial enlarged schematic diagram of part B in FIG. 1 .

In the picture: 1 filter box, 2 through holes, 3 filter plates, 4 connecting pipes, 5 caps, 6 liquid inlet holes, 7 cover plates, 8 clamping posts, 9 first magnet blocks, 10 grooves, 11 second magnets Block, 12 rotary rod, 13 first helical gear, 14 second helical gear, 15 threaded rod, 16 nut, 17 spring, 18 splint, 19 chute, 20 slider, 21 quantitative box, 22 piston plate, 23 baffle , 24 push rods, 25 liquid outlet pipes, 26 one-way valves, 27 L-shaped support blocks.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the implementations. example.

1-3, a stem cell conditioned medium filtering device includes a filter box 1, a capping mechanism is rotatably connected to the side wall of the filter box 1, one end of the capping mechanism is movably connected to the side wall of the filter box 1, The cover mechanism includes a cover 5 that is rotatably connected to the side wall of the filter box 1, wherein the cover 5 has a certain sealing function, and the upper end of the cover 5 is provided with a liquid inlet hole 6, and the culture medium enters the filter from the liquid inlet hole 6. In the box 1, the liquid inlet 6 is provided with a cover plate 7, the lower end of the cover 5 is fixedly connected with a clamping column 8, the lower end of the clamping column 8 is fixedly connected with a first magnetic block 9, and the side wall of the filter box 1 is provided with There is a groove 10, the inner bottom end of the groove 10 is fixedly connected with a second magnet block 11, the first magnet block 9 is movably connected with the second magnet block 11, wherein both the first magnet block 9 and the second magnet block 11 are mutual attraction.

The left and right ends of the filter box 1 are provided with through holes 2 , and a drive mechanism is provided through the two through holes 2 . One end of the shaft is fixedly connected with a first helical gear 13, wherein when the rotating rod 12 is rotated, the first helical gear 13 will rotate, and the first helical gear 13 is engaged with the moving mechanism.

The inner bottom end of the filter box 1 is rotatably connected with two moving mechanisms. The driving mechanism is engaged with the moving mechanism. The moving mechanism includes a second helical gear 14 rotatably connected to the inner bottom end of the filter box 1. The gears 14 are meshed, the upper end of the second helical gear 14 is fixedly connected with a threaded rod 15, and a nut 16 is sleeved on the threaded rod 15. The wall is provided with a vertical chute, wherein the vertical chute is provided with a matching sliding block, wherein the sliding block is connected with the nut 16, and the vertical sliding groove and the sliding block can help the nut 16 to move freely upward or downward. Moving down, the side wall of the nut 16 is fixedly connected with the L-shaped support block 27, the upper end of the L-shaped support block 27 is fixedly connected with the spring 17, the upper end of the spring 17 is fixedly connected with the splint 18, and the splint 18 is connected with the L-shaped support block 27. The side walls are slidingly attached.

The upper and lower ends of the filter plate 3 are respectively movably connected with the splint 18 and the L-shaped support block 27. The side wall of the L-shaped support block 27 is provided with a chute 19, and the sliding groove 19 is slidably connected with a matching slider 20. The slider 20 is connected with the splint 18, and the splint 18 is slidably connected with the chute 19. The spring 17 can reset the splint 18. When the filter plate 3 needs to be clamped, the filter plate 3 is directly placed on the splint 18 and the L-shaped support. in block 27.

A filter plate 3 is arranged between the two moving mechanisms, and the filter plate 3 is respectively movably connected with the two moving mechanisms. The lower end of the filter box 1 is provided with a quantitative mechanism, and the quantitative mechanism includes a quantitative box 21 arranged at the lower end of the filter box 1, wherein The quantitative box 21 is engraved with a volume gauge, the quantitative box 21 is connected with the filter box 1 through the connecting pipe 4, the inner side wall of the quantitative box 21 is slidably connected with two piston plates 22, and the upper ends of the two piston plates 22 are fixedly connected with baffle 23.

The side walls of the two piston plates 22 are fixedly connected with push rods 24, one end of the two push rods 24 passes through the side wall of the quantitative box 21 and extends to the outside, wherein the two push rods 24 are respectively connected to the side of the quantitative box 21. The walls are slidingly connected, the liquid outlet of the quantitative box 21 is fixedly installed with a liquid outlet pipe 25, and the side wall of the liquid outlet pipe 25 is fixedly installed with a one-way valve 26. The quantitative mechanism is connected to the filter box 1 through the connecting pipe 4.

In the present invention, when it is necessary to cover the filter box 1, the cover 5 is directly covered on the filter box 1 first. By directly inserting it into the groove 10, the first magnet block 9 is connected with the second magnet block 11 at this time, so that the purpose of sealing the filter box 1 can be achieved.

When the filter plate 3 needs to be replaced, the rotating rod 12 is first rotated. When the rotating rod 12 rotates, the first helical gear 13 rotates. When the first helical gear 13 rotates, the second helical gear 14 rotates. When the second helical gear 14 rotates, the threaded rod 15 will rotate. When the threaded rod 15 rotates, and since the nut 16 is slidably connected to the side wall of the filter box 1, the nut 16 can move up and down freely on the threaded rod 15. , when the nut 16 moves to the upper end of the threaded rod 15, pull the splint 18 at the same time. When the splint 18 is pulled, the filter plate 3 can be taken out, and then the new filter plate 3 is placed on the L-shaped support block 27, Then the clamping plate 18 is released. When the clamping plate 18 is released, the clamping plate 18 will be tightly contacted with the filter plate 3 , so as to achieve the purpose of replacing the filter plate 3 .

When quantitative measurement is required, first push the push rod 24 directly, when the push rod 24 moves, the piston plate 22 will move, and when the piston plate 22 moves, the baffle 23 will move to the bottom of the connecting pipe 4, In this way, the flow rate of the connecting pipe 4 will be reduced, so as to achieve the purpose of being able to be quantified.

The above description is only a preferred embodiment of the present invention, but the protection scope of the present invention is not limited to this. Equivalent replacement or modification of the new technical solution and its utility model concept shall be included within the protection scope of the present utility model.

Claims (6)

1. A stem cell conditioned medium filtering device, comprising a filter box (1), characterized in that a capping mechanism is rotatably connected on the side wall of the filter box (1), and one end of the capping mechanism is connected to the filter box. The side walls of (1) are movably connected, the left and right ends of the filter box (1) are provided with through holes (2), and two through holes (2) are provided with a driving mechanism, and the filter box (1) is provided with a driving mechanism. The inner bottom end of (1) is rotatably connected with two moving mechanisms, the driving mechanism is engaged with the moving mechanism, and a filter plate (3) is arranged between the two moving mechanisms, and the filter plate (3) is respectively connected with The two moving mechanisms are movably connected, the lower end of the filter box (1) is provided with a quantitative mechanism, and the quantitative mechanism is connected with the filter box (1) through a connecting pipe (4). 2. A stem cell conditioned medium filtering device according to claim 1, wherein the capping mechanism comprises a cap (5) rotatably connected to the side wall of the filter box (1), the capping The upper end of (5) is provided with a liquid inlet hole (6), a cover plate (7) is arranged through the liquid inlet hole (6), and a clamping column (8) is fixedly connected to the lower end of the cover (5), A first magnetic block (9) is fixedly connected to the lower end of the clamping column (8), a groove (10) is provided on the side wall of the filter box (1), and the inner bottom end of the groove (10) is A second magnet block (11) is fixedly connected, and the first magnet block (9) is movably connected with the second magnet block (11). 3. A stem cell conditioned medium filtering device according to claim 2, wherein the driving mechanism comprises a rotating rod (12) disposed through the through hole (2), and the rotating rod (12) A first helical gear (13) is fixedly connected to one end inside the filter box (1), and the first helical gear (13) is engaged with the moving mechanism. 4. A stem cell conditioned medium filtering device according to claim 3, wherein the moving mechanism comprises a second helical gear (14) rotatably connected to the inner bottom end of the filter box (1), the first The helical gear (13) meshes with the second helical gear (14), the upper end of the second helical gear (14) is fixedly connected with a threaded rod (15), and a nut (16) is sleeved on the threaded rod (15). ), the side wall of the nut (16) is slidably connected with the side wall of the filter box (1), the side wall of the nut (16) is fixedly connected with an L-shaped support block (27), and the L-shaped support block The upper end of (27) is fixedly connected with a spring (17), the upper end of the spring (17) is fixedly connected with a splint (18), and the splint (18) is slidably connected to the side wall of the L-shaped support block (27). , the upper and lower ends of the filter plate (3) are respectively movably connected with the splint (18) and the L-shaped support block (27). 5. A stem cell conditioned medium filtering device according to claim 4, wherein a chute (19) is provided on the side wall of the L-shaped support block (27), and the chute (19) A sliding block (20) matched therewith is slidably connected inside, the sliding block (20) is connected with the clamping plate (18), and the clamping plate (18) is slidably connected with the chute (19). 6. A stem cell conditioned medium filtering device according to claim 4, wherein the quantitative mechanism comprises a quantitative box (21) arranged at the lower end of the filter box (1), the quantitative box (21) It is connected with the filter box (1) through the connecting pipe (4), the inner side wall of the quantitative box (21) is slidably connected with two piston plates (22), and the upper ends of the two piston plates (22) are fixed A baffle plate (23) is connected, and push rods (24) are fixedly connected to the side walls of the two piston plates (22). The side wall extends to the outside, a liquid outlet pipe (25) is fixedly installed on the liquid outlet of the quantitative box (21), and a one-way valve (26) is fixedly installed on the side wall of the liquid outlet pipe (25).