CN208520805U - A kind of denaturing gradient gel electrophoresis equipment - Google Patents

Abstract

The utility model relates to a kind of denaturing gradient gel electrophoresis equipment, including the electrophoresis inner core being movably inserted in electrophoresis kerve and controller, the upper cover being hinged on sealing partition, circulating pump is installed in the controller, the inlet of the circulating pump is penetrated through by the kerve inlet on feed liquor hose and electrophoresis kerve and is connected, on the one hand the liquid outlet of the circulating pump is connected by going out liquid hose with the liquid outlet perforation being provided on electrophoresis kerve, on the other hand it penetrates through and connects with minim pipette, the minim pipette can be inserted into electrophoresis inner core, and electrophoresis inner core has discharge outlet.The electrophoresis equipment is compact-sized rationally, temperature control is accurate, carrying easy to disassemble and the flowing that can control buffer, guarantee ion concentration are balanced.

Description

A kind of denaturing gradient gel electrophoresis equipment

Technical field

The utility model relates to denaturing gradient gel electrophoresis technique fields, are specifically related to a kind of denaturing gradient gel electrophoresis Equipment.

Background technique

The utility model relates to a kind of modified electrophoresis tanks by Patent No. ZL201320347698.5 comprising electrophoresis tank sheet Body, lid and pedestal；Wherein, a temperature sensor is equipped in the electrophoresis tank ontology；The lid is articulated in electrophoresis tank sheet The side of body which is provided with a camera and a fan Hot-air fan；The pedestal is located at the bottom of electrophoresis tank ontology, which is provided with One resistive heating device；The resistive heating device, radiator fan are electrically connected with temperature sensor respectively, and by temperature sensor Control.The Bioexperiment electrophoresis tank is intrinsic to electrophoresis tank by the resistive heating device for being mounted on electrophoresis tank body bottom portion The heating method that buffer is heated cannot be evenly heated the intrinsic buffer of electrophoresis tank, in the electrophoresis tank ontology Buffer can not effectively be recycled.Therefore, the temperature control accuracy of such electrophoresis tank is not high.In addition, though the electricity Swimming slot can be by the resistive heating device that connect with temperature sensor, radiator fan jointly to selecting with the intrinsic buffer of material Carry out temperature control.But the control precision of such temperature control method is not high, control efficiency is lower.

The Chinese utility model patent application of Patent No. ZL00238873.1 discloses a kind of Vertial electrophorestic tank, the electrophoresis Slot includes groove body, glue room and a buffer temperature control circulation system.Groove body is upper opening cuboid, is divided into rectangular spacer Lower two electrode chambers, are provided with rectangular glue room socket on partition, are provided with loading hole with glue room socket corresponding position in slot cover；Groove body It is connected there are four interface with buffer temperature control circulation system on bottom plate.Temperature control in the buffer temperature control circulation system of the electrophoresis tank Component is connected by the perforation of the groove body bottom of pipeline and electrophoresis tank and carries out loop temperature-control to buffer by circulating pump.By upper Although the buffer temperature controlling circulating system stated can carry out temperature control to the intracorporal buffer of slot to a certain extent.But pass through Such temperature control method can not play good temperature control effect to the intracorporal circulation fluid of slot, be followed by circulating pump to buffer When ring, the flow velocity of buffer is larger, be easy to cause equipment unstable.In addition, the buffer temperature control circulation system of the electrophoresis tank is set It sets in the bottom of groove body, the volume of electrophoresis tank of increase is unfavorable for carrying and dismounts.

Utility model content

To overcome above-mentioned shortcomings and deficiencies existing in the prior art, the utility model provide it is a kind of it is compact-sized rationally, Temperature control is accurate, carrying easy to disassemble and a kind of denaturing gradient gel electrophoresis equipment that can guarantee ion concentration equilibrium.

To achieve the above object, the technical solution of the utility model is: a kind of denaturing gradient gel electrophoresis equipment, including can It is loosely inserted into electrophoresis inner core and controller in electrophoresis kerve, the upper cover being hinged on sealing partition, in the controller Circulating pump is installed, the inlet of the circulating pump is penetrated through by the kerve inlet in feed liquor hose and electrophoresis kerve to be connected, The liquid outlet of the circulating pump is connected by going out liquid hose with the liquid outlet perforation being provided in electrophoresis kerve, on the liquid outlet Perforation is connected with minim pipette, and the minim pipette is movably inserted in electrophoresis inner core, and electrophoresis inner core has discharge outlet.

It is alternatively preferably, the inlet of the circulating pump passes through in feed liquor hose and electrophoresis kerve Kerve inlet penetrate through connection, the liquid outlet of the circulating pump passes through the liquid outlet that liquid hose Yu be provided in electrophoresis kerve Perforation connects, and perforation is connected with minim pipette on the liquid outlet, and the minim pipette is movably inserted in electrophoresis inner core.

Preferably, be also equipped on the controller temperature sensor in pluggable electrophoresis kerve, heating device and Water level sensor.

In any of the above-described scheme preferably, the heating device, temperature sensor, water level sensor by route with The microprocessor being mounted in controller is electrically connected.

In any of the above-described scheme preferably, the heating device is electrically heated rod, and described heating rod is U-shaped knot Structure.

In any of the above-described scheme preferably, the minim pipette is through upper cover.

In any of the above-described scheme preferably, it is equipped on the cover corresponding with the electrode tip on electrophoresis inner core Two power interfaces, the power interface connect by power supply line with peripheral power supply.

In any of the above-described scheme preferably, the handle exterior contour phase with electrophoresis inner core is offered on the cover The opening of cooperation, the handle may pass through the upper cover.

In any of the above-described scheme preferably, control panel, switch, exhaust fan are installed on the side plate of the controller And serial line interface.

In any of the above-described scheme preferably, the distance of bottom surface of the kerve inlet to electrophoresis kerve is less than electrophoresis The distance of liquid outlet on kerve to the bottom surface of electrophoresis kerve.

In any of the above-described scheme preferably, the discharge outlet is arranged on the end plate of electrophoresis inner core.

It compared with prior art utility model has the advantages that can be to slow in the deformation gradient gel electrophoresis equipment Fliud flushing is recycled and is heated, and realizes accurately temperature control；The power on/off to electrophoresis inner core can be achieved by the folding of upper cover, Safety is higher.In addition, the denaturing gradient gel electrophoresis device structure compact and reasonable, it is easy to disassemble carrying and can guarantee ion Equalization of concentration.

Detailed description of the invention

Fig. 1 is to illustrate according to the stereochemical structure of one preferred embodiment of denaturing gradient gel electrophoresis equipment of the utility model Figure.

Fig. 2 is after opening according to the upper cover of the denaturing gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model Structural schematic diagram.

Fig. 3 is to illustrate according to the deployed configuration of the deformation gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model Figure.

Fig. 4 is the gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of ceasing to be faithful according to the utility model without electrophoresis inner core Structural schematic diagram.

Fig. 5 is the main view according to the denaturing gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model.

Fig. 6 is to show according to the vertical view graph structure of the denaturing gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model It is intended to.

Fig. 7 is the left view structural representation according to the denaturing gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model Figure.

Fig. 8 is the right view structural representation according to the denaturing gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model Figure.

Fig. 9 is the structure according to controller in the denaturing gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model Schematic diagram.

Figure 10 is the knot according to electrophoresis inner core in the denaturing gradient gel electrophoresis equipment embodiment illustrated in fig. 1 of the utility model Structure schematic diagram.

Specific embodiment

Explanation is further elaborated to the embodiments of the present invention with reference to the accompanying drawing；

Embodiment 1:

As Figure 1-10 shows, a kind of deformation gradient gel electrophoresis equipment including electrophoresis kerve 1, is placed in electrophoresis kerve 1 Electrophoresis inner core 2 and controller 3.Sealing partition 4 is provided in electrophoresis kerve 1.It seals partition 4 and passes through its bottom edge and two sides With in electrophoresis kerve 1 ground and two sides be tightly connected.Electrophoresis inner core 2 and controller 3 is mobilizable is inserted in electrophoresis In kerve 1.Upper cover 5 is movably hinged in sealing the upper of partition 4.In the present embodiment, upper cover 5 it is openable and closable be hinged on it is close On the top margin for insulating plate 4, the purpose of sealing electrophoresis kerve 1 can be realized by overturning upper cover 5.And it can realize to electrophoresis inner core 2 Power on/off.Further, the opening matched with the exterior contour of the handle 23 of electrophoresis inner core 2, institute are offered in upper cover 5 The handle 23 of electrophoresis inner core 2 can be accommodated by stating opening.Upper cover 5, which can be buckled in, to be connect at the top of electrophoresis kerve 1 with 2 activity of electrophoresis inner core Touching.There are two electrode tips 26 for setting on electrophoresis inner core 2.Two power supplys corresponding with electrode tip 26 are provided in upper cover 5 to connect Mouth 24, power interface 24 can snap on electrode tip 26, realize that the power supply line 17 connecting with power interface 24 connects.Work as operator When opening upper cover 5, power interface 24 and electrode tip 26 are detached from, and realize the power-off to electrophoresis inner core 2.Further, operator can Electrophoresis inner core 2 is taken out out of electrophoresis kerve 1 by handle 23, realizes and carries.When operator closes upper cover 5, power interface 24 connect with electrode tip 26, realize the energization to electrophoresis inner core 2.

In the present embodiment, electrophoresis kerve 1 is the groove of rectangular configuration.It is arranged on the sealing partition 4 in electrophoresis kerve 1 There are kerve inlet 8 and liquid outlet 11.The distance of kerve inlet 8 to the bottom surface of electrophoresis kerve 1 in electrophoresis kerve 1 is less than electricity Distance of the liquid outlet 11 swum on kerve 1 to the bottom surface of electrophoresis kerve 1.Controller 3 is mobilizable be inserted in electrophoresis kerve 1 and Circulating pump is installed in controller 3.The circulating pump has inlet 6 and liquid outlet.The inlet 6 of circulating pump is soft by feed liquor Kerve inlet 8 in pipe 7 and electrophoresis kerve 1, which penetrates through, to be connected, the liquid outlet one side hose and electrophoresis kerve 1 of the circulating pump Interior liquid outlet 11 penetrates through connection, on the other hand the perforation connection of minim pipette 12 by being fixed on the through-hole.In electrophoresis Discharge outlet 13 is offered on the end plate 22 of core 2.The top, middle part, lower part of end plate 22 can be arbitrarily arranged in discharge outlet 13.Row The setting at the mouth of a river 13 can be achieved that the buffer in electrophoresis inner core 2 is discharged, into electrophoresis kerve 1.

Deformation gradient gel electrophoresis equipment in the present embodiment by circulating pump in controller 3, is arranged in electrophoresis kerve Kerve inlet 8, liquid outlet 11 and discharge outlet 13 in 1 are realized to slow in the buffer and electrophoresis inner core 2 in electrophoresis kerve 1 Fliud flushing is recycled, and ensure that the ion concentration of buffer in electrophoresis inner core 2 and electrophoresis kerve 1.

Control panel 18, switch 19, exhaust fan 20 and serial line interface 21 are also equipped on controller 3.Control panel 18 can Show the temperature information and ion concentration information of the buffer in current electrophoresis kerve 1.Switch 19 can be realized to the logical of controller 3 Power-off.Exhaust fan 20, which can be realized, radiates to the component in controller 3.Controller 3 can realize data by serial line interface Communication function.Temperature sensor 14, heating device and the water in pluggable electrophoresis kerve 1 are also equipped in the bottom of controller 3 Level sensor 16.The heating device, temperature sensor 14, water level sensor 16 by route and are mounted in controller 3 Microprocessor is electrically connected.Temperature sensor 14 is used to detect the real time temperature of buffer in electrophoresis kerve 1 and will measure Information passes to the microprocessor being placed in controller 3.Equally, water level sensor 16 is used to detect the buffering in electrophoresis kerve 1 The liquid level information of liquid.The informix that information that the microprocessor is measured according to water level sensor 16, temperature sensor measure Control that heating time of the heating device, the revolving speed of motor is realized to slow in electrophoresis kerve 1 in the circulating pump in real time The temperature of fliud flushing controls and the flow velocity of buffer.In the present embodiment, the heating device is electrically heated rod 15, the electric heating Stick 15 is U-shaped structure.

Embodiment 2:

As Figure 1-10 shows, a kind of denaturing gradient gel electrophoresis equipment, including being movably inserted in electrophoresis kerve 1 Electrophoresis inner core 2 and controller 3 are hinged on the upper cover 5 sealed on partition 4, are equipped with circulating pump, the circulating pump in controller 3 Inlet 6 penetrated through and connect by the kerve inlet 8 in feed liquor hose 7 and electrophoresis kerve 1, the liquid outlet of the circulating pump is logical It crosses out liquid hose to connect with the perforation of liquid outlet 11 being provided in electrophoresis kerve 1, perforation is connected with dropping liquid on the liquid outlet 11 Pipe 12, the minim pipette 12 are movably inserted in electrophoresis inner core 2, and electrophoresis inner core 2 has discharge outlet 13.

Embodiment 3:

As Figure 1-10 shows, with embodiment 1 the difference is that kerve inlet 8 and liquid outlet 11 in the present embodiment are equal It is arranged on the side plate of electrophoresis kerve 1.

It should be noted that the scope of the technical solution of the denaturing gradient gel electrophoresis equipment of the utility model includes but not Any combination being limited between each part mentioned above.Any combination including embodiment 1-3.

Although the utility model, those skilled in the art have shown and described referring particularly to its preferred embodiment It is appreciated that the various changes that can be made in form and details are practical new without departing from sheet described in the appended claims The range of type.For example, setting position, kerve inlet and the setting of liquid outlet of discharge outlet 13 etc. on electrophoresis inner core 2.More than It is described in detail in conjunction with specific embodiment of the utility model, but is not limitations of the present invention.All foundations are originally Any simple modification made to the above embodiment of the technical spirit of utility model, still falls within technical solutions of the utility model Range.

Claims (10)

1. a kind of denaturing gradient gel electrophoresis equipment, including the electrophoresis inner core being movably inserted in electrophoresis kerve and control Device, the upper cover being hinged on sealing partition, it is characterised in that: circulating pump, the inlet of the circulating pump are equipped in controller Penetrated through and connected by the kerve inlet in feed liquor hose and electrophoresis kerve, the liquid outlet of the circulating pump by go out liquid hose with The liquid outlet perforation being provided in electrophoresis kerve connects, and perforation is connected with minim pipette on the liquid outlet, and the minim pipette can live It is inserted in electrophoresis inner core dynamicly, electrophoresis inner core has discharge outlet.

2. denaturing gradient gel electrophoresis equipment as described in claim 1, it is characterised in that: being also equipped on the controller can Temperature sensor, heating device and the water level sensor being inserted into electrophoresis kerve.

3. denaturing gradient gel electrophoresis equipment as claimed in claim 2, it is characterised in that: the heating device, temperature sensing Device, water level sensor are electrically connected by route with the microprocessor being mounted in controller.

4. denaturing gradient gel electrophoresis equipment as claimed in claim 2, it is characterised in that: the heating device is electric heating Stick, the electrically heated rod are U-shaped structure.

5. denaturing gradient gel electrophoresis equipment as described in claim 1, it is characterised in that: the minim pipette is through upper cover.

6. denaturing gradient gel electrophoresis equipment as claimed in claim 1 or 5, it is characterised in that: be equipped on the cover Two power interfaces corresponding with the electrode tip on electrophoresis inner core, the power interface are connected by power supply line and peripheral power supply It connects.

7. denaturing gradient gel electrophoresis equipment as claimed in claim 6, it is characterised in that: offer on the cover and electricity The opening that the handle exterior contour of swimming inner core matches, the handle may pass through the upper cover.

8. denaturing gradient gel electrophoresis equipment as described in claim 1, it is characterised in that: installed on the side plate of the controller There are control panel, switch, exhaust fan and serial line interface.

9. denaturing gradient gel electrophoresis equipment as described in claim 1, it is characterised in that: the kerve inlet to electrophoresis bottom The distance of the bottom surface of slot is less than the liquid outlet on electrophoresis kerve to the distance of the bottom surface of electrophoresis kerve.

10. denaturing gradient gel electrophoresis equipment as described in claim 1, it is characterised in that: the discharge outlet is arranged in electrophoresis On the end plate of inner core.