CN208218879U - A kind of Tissue Culture Flask convenient for sampling - Google Patents

Abstract

A cell culture bottle that is convenient for sampling, relates to cell culture experiment consumables, including an upper bottle body, a lower bottle body and a bottleneck, the upper bottle body has a trapezoidal structure, the lower bottle body has a rectangular parallelepiped structure, the bottom surface of the upper bottle body and the lower bottle body Connection, the top surface of the upper bottle body is connected to the bottleneck, the inner side of the side part connected to the hypotenuse part of the lower bottle body and the upper bottle body is inclined, and the side part is from the bottom surface of the lower bottle body to the top surface of the lower bottle body It gradually narrows, and there is a double-layer round cover connected to the neck of the bottle. The double-layer round cover includes an inner cover and an outer cover. There is a circular hole on the upper end of the inner cover. The detachable connection is connected to the upper end of the inner cover and the rubber plug and the circular hole are closed, and the inner cover is detachably connected to the bottle neck. The utility model provides the convenience of taking out the liquid from the cell culture bottle, and can effectively prevent the inside of the culture bottle from being polluted. Sampling of cell culture flasks.

Description

A convenient cell culture flask for sampling

technical field

The utility model relates to consumables for cell culture experiments, in particular to a cell culture bottle which is convenient for sampling.

Background technique

Most of the existing cell culture bottles include an upper bottle body, a lower bottle body and a bottleneck, the upper bottle body is a trapezoidal structure, the lower bottle body is a cuboid structure, the bottom surface of the upper bottle body is connected with the lower bottle body, and the top surface of the upper bottle body It is connected with the bottle neck, the bottle neck is threaded, and the bottle cap is connected with the bottle neck.

In order to make it easier for the pipette to take out the liquid from the cell culture bottle, the bottle body on the existing cell culture bottle has a trapezoidal structure. Put the culture bottle on, and use a pipette to take out the liquid inside the cell culture bottle from the neck of the cell, but the cell When the solution in the culture bottle is less, the liquid in the culture bottle is not easy to flow to the vicinity of the upper bottle body, which makes it inconvenient to take out the reagents, culture fluid, etc. in the cell culture bottle.

Moreover, in the cell culture process, it is necessary to add or remove liquid to or from the culture bottle through a pipette many times. However, the existing cell culture bottle has a large bottleneck, which may cause the inside of the culture bottle to be easily polluted when the bottle cap is opened to add or take out liquid.

Contents of the invention

In view of the above situation, in order to solve the problem that when the solution in the existing cell culture bottle is less, the liquid in the culture bottle is not easy to flow to the vicinity of the bottle body, making it inconvenient to take out the reagents and culture fluid in the cell culture bottle, and the existing cell culture bottle The bottleneck is relatively large, which may cause contamination inside the culture bottle when the cap is opened to add or take out liquid. The utility model provides a convenient way to take out the liquid from the cell culture bottle, and can effectively prevent the inside of the culture bottle from being polluted and facilitate sampling. Cell culture flasks.

The technical scheme is: including the upper bottle body, the lower bottle body and the bottleneck, the upper bottle body has a trapezoidal structure, the lower bottle body has a cuboid structure, the bottom surface of the upper bottle body is connected with the lower bottle body, and the top surface of the upper bottle body is connected with the bottleneck , the inner side of the side part connecting the lower bottle body and the hypotenuse part of the upper bottle body is inclined, and the side part gradually narrows from the bottom surface of the lower bottle body to the top surface of the lower bottle body. Cover, the double-layer round cover includes an inner cover and an outer cover. There is a circular hole on the upper end of the inner cover, and a rubber plug is detachably connected to the inside of the circular hole. The outer cover is detachably connected to the upper end of the inner cover and the rubber plug And the circular hole is closed, and the inner cap is detachably connected with the bottle neck.

Preferably, both the inner cover and the outer cover are cylindrical with an opening at the lower end, a section of external thread is provided on the side of the inner cover near the upper end surface, and an internal thread is provided on the inner side of the side of the outer cover, and the outer cover is connected by threads. The upper end of the inner cover and the inner side of the side of the inner cover are provided with internal threads, and the inner cover and the bottle neck are connected by threads.

Preferably, there are three circular holes, and the diameters of the three circular holes are respectively equal to the outer diameters of pipettes with specifications of 1 mL, 10 mL and 20 mL.

Preferably, a pull ring is provided at the upper end of the rubber plug, one end of the pull ring is fixedly connected to the rubber plug, and the other end of the pull ring is fixedly connected to the upper surface of the inner cover.

Preferably, the upper bottle body, the lower bottle body and the bottle neck are all made of transparent polystyrene material.

Preferably, scales are provided on the lower bottle body.

The utility model provides a cell culture bottle which is convenient for sampling, and compared with the prior art, the beneficial effects are:

1. The inner side of the side part connecting the lower bottle body and the beveled part of the upper bottle body is inclined. When taking the liquid, the liquid will flow to the upper bottle body, which is convenient for taking out the liquid inside the cell culture bottle, especially in When there is less liquid in the cell culture bottle, it is convenient for taking liquid;

2. Set up a double-layer round cap. When adding or removing liquid, only one layer of cap needs to be opened to avoid opening the entire cap, which can effectively prevent the inside of the cell culture bottle from being polluted;

3. Set three circular holes, the diameters of which are equal to the outer diameters of pipettes with specifications of 1mL, 10mL, and 20mL respectively. Users can choose pipettes of different specifications according to the amount of liquid inside the cell culture bottle. In the process of taking liquid, the pipette is suitable for the size of the circular hole, which further reduces the contact between the inside of the cell culture bottle and the outside world, thereby further preventing the inside of the cell culture bottle from being polluted;

4. Set the pull ring, the pull ring is fixedly connected with the inner cover and the rubber plug, which is convenient for removing the rubber plug. At the same time, during the process of taking liquid or adding liquid, it can prevent the rubber plug from being directly in contact with the experimental surface and being polluted, and can also prevent the rubber plug from being polluted. The loss of will bring trouble to the cell culture process;

5. The upper body, the lower body and the neck of the bottle are made of transparent polystyrene material, which is convenient for observing the growth and adhesion of cells in the bottle during the cell culture process.

Description of drawings

Fig. 1 the utility model provides a kind of cell culture bottle that is convenient to take a sample and vertically places the direct view;

Fig. 2 the utility model provides a kind of cell culture flask that is convenient to take a sample horizontally placed direct view;

Fig. 3 The utility model provides a top view of the inner cover;

Fig. 4 The utility model provides a direct view of the inner cover;

Fig. 5 The utility model provides a direct view of the outer cover;

Fig. 6 The utility model provides a structural diagram of the rubber plug.

In the figure: 1 upper bottle body, 2 lower bottle body, 3 bottleneck, 4 beveled edge, 5 side edge, 6 double-layer round cap, 7 inner cap, 8 outer cap, 9 circular hole, 10 rubber plug, 11 pull ring.

Detailed ways

Now in conjunction with accompanying drawing, the utility model is described in further detail. The accompanying drawing is a simplified schematic diagram, which only illustrates the basic structure of the utility model in a schematic way, so it only shows the configuration related to the utility model.

Embodiment 1. As shown in FIGS. 1 and 2 , a cell culture bottle convenient for sampling includes an upper bottle body 1 , a lower bottle body 2 and a bottleneck 3 . The upper bottle body 1 is a trapezoidal structure, and the lower bottle body 2 is a cuboid structure, and the bottom surface of the upper bottle body 1 is connected with the lower bottle body 2. The top surface of the upper bottle body 1 is connected with the bottleneck 3 . The upper bottle body 1, the lower bottle body 2 and the bottleneck 3 are integrally formed. The cell culture flask can be placed vertically or horizontally on the support surface.

The inner side of the side portion 5 where the lower bottle body 2 is connected with the hypotenuse portion 4 of the upper bottle body 1 is inclined. The side portion 5 gradually narrows from the bottom surface of the lower bottle body 2 to the top surface of the lower bottle body 2 . The angle between the inner side of the side portion 5 and the vertical plane is 5-10 degrees. The inner side of the side part 5 is set to be inclined. When taking liquid from the cell culture bottle, the cell culture bottle is laid down. The side part 5 is used as the bottom surface to support the cell culture bottle, and the liquid will flow from the cell culture bottle to one place on the body of the bottle. At this time, it is convenient to put the pipette through the bottleneck 3 into the upper bottle body 1 to take the liquid, especially when there is less liquid inside the cell culture bottle, it is convenient for taking the liquid.

A double-layer round cover 6 is connected to the bottleneck 3 . As shown in FIGS. 3-5 , the double-layer round cover 6 includes an inner cover 7 and an outer cover 8 . Both the inner cover 7 and the outer cover 8 are made of plastic material. A circular hole 9 is provided on the upper end surface of the inner cover 7 . The circular hole 9 is adapted to the size of the pipette. The circular hole 9 runs through the upper end surface of the inner cover 7 . The circular hole 9 inside is detachably connected with a rubber stopper 10. As shown in FIG. 6 , the size of the rubber stopper 10 matches the size of the circular hole 9 . The outer cover 8 is detachably connected to the upper end of the inner cover 7 and seals the rubber plug 10 and the circular hole 9 . The inner cover 7 is detachably connected with the bottle neck 3 . When adding or taking out the liquid, only need to open the outer cover 8, and use the pipette to pass through the circular hole 9 into the inside of the cell culture bottle to take the liquid, avoiding opening the entire bottle cap, which can effectively prevent the inside of the cell culture bottle from being polluted.

Embodiment 2. On the basis of the above embodiments, further, the inner cover 7 and the outer cover 8 are cylindrical with openings at their lower ends. A section of external thread is provided on the outer side of the inner cover 7 near the upper end surface. The inner side of the side of the outer cover 8 is provided with an internal thread. The thread on the inside of the outer cover 8 is compatible with a section of external thread on the outside of the side of the inner cover 7 . The outer cover 8 is threadedly connected to the upper end of the inner cover 7 .

The inner side of the inner cover 7 is provided with an internal thread. Inner cap 7 is connected with bottle neck 3 by thread. The opening of the inner cover 7 and the outer cover 8 is facilitated by threaded connection, which provides convenience for taking out or adding liquid.

Embodiment 3. On the basis of the above embodiments, further, there are three circular holes 9, and the diameters of the three circular holes 9 are respectively equal to the outer diameters of pipettes with specifications of 1mL, 10mL, and 20mL. Pipettes of different specifications are adapted to the size of the circular hole 9 to increase the flexibility of use of the cell culture bottle, and pipettes of different specifications are suitable to the size of the circular hole 9 during the liquid extraction process It can further reduce the contact between the inside of the cell culture bottle and the outside world, thereby further preventing the inside of the cell culture bottle from being polluted.

Embodiment 4 On the basis of the above embodiments, further, a pull ring 11 is provided at the upper end of the rubber plug 10 . One end of the pull ring 11 can be fixedly connected with the rubber stopper 10 with superglue. The other end of the pull ring 11 can be fixedly connected with the upper surface of the inner cover 7 by superglue. The pull ring 11 is provided to facilitate the removal of the rubber stopper 10, and at the same time prevent the rubber stopper 10 from being directly in contact with the experimental surface and be polluted during the process of taking or adding liquid, and can also prevent the loss of the rubber stopper 10 from causing trouble to the cell culture process.

Embodiment 5. On the basis of the above embodiments, further, the upper bottle body 1, the lower bottle body 2 and the bottleneck 3 are all made of transparent polystyrene material. It is convenient to observe the growth and adhesion of cells in the bottle during the cell culture process.

Embodiment 6. On the basis of the above embodiments, further, the lower bottle body 2 is provided with a scale, which is not marked in the scale figure. When the cell culture bottle can be placed vertically on the support surface, the scale starts from the top of the lower bottle body 2. to bottom settings. The right amount of liquid can be added accurately.

The above-mentioned embodiments are only examples for clear description, and are not intended to limit the implementation. For those of ordinary skill in the art, other changes or changes in different forms can be made on the basis of the above description. The changes or variations derived therefrom are still within the protection scope of the utility model creation.

Claims (6)

1. a kind of Tissue Culture Flask convenient for sampling, including upper body (1), lower body (2) and bottleneck (3), upper body (1) is ladder Shape and structure, lower body (2) are rectangular parallelepiped structure, and the bottom surface of upper body (1) is connect with lower body (2), the top surface of upper body (1) It is connect with bottleneck (3), which is characterized in that the side that the lower body (2) is connected with the bevel edge portion (4) of the upper body (1) The inside in portion (5) is oblique, and the side portion (5) from lower body (2) bottom surface to lower body (2) top surface gradually Narrow, be connected with the double-deck dome (6) on the bottleneck (3), the bilayer dome (6) includes inner cover (7) and outer cover (8), described Inner cover (7) upper surface is equipped with round hole (9), and what can be dismantled inside the round hole (9) is connected with rubber stopper (10), the outer cover (8) it is removably attachable to the inner cover (7) upper end and closes the rubber stopper (10) and the round hole (9), it is described interior Lid (7) is removably connect with the bottleneck (3).

2. a kind of Tissue Culture Flask convenient for sampling according to claim 1, which is characterized in that the inner cover (7) and outer Lid (8) is that lower end is equipped with the cylindric of opening, and one end of close upper surface is equipped with one section of outer spiral shell on the outside of inner cover (7) side Line, outer cover (8) the side inside are equipped with internal screw thread, and the outer cover (8) is threadedly attached in the inner cover (7) upper end, institute It states and is equipped with internal screw thread on the inside of inner cover (7) side, the inner cover (7) is connected through a screw thread with the bottleneck (3).

3. a kind of Tissue Culture Flask convenient for sampling according to claim 1, which is characterized in that the round hole (9) is Three, the diameter of three round holes (9) is respectively equal to the outer diameter for the pipette that specification is 1mL, 10mL, 20mL.

4. a kind of Tissue Culture Flask convenient for sampling according to claim 1, which is characterized in that on the rubber stopper (10) End is equipped with pull ring (11), and described pull ring (11) one end is fixedly connected with the rubber stopper (10), pull ring (11) other end and Inner cover (7) upper surface is fixedly connected.

5. a kind of Tissue Culture Flask convenient for sampling according to claim 1, which is characterized in that the upper body (1), under Body (2) and bottleneck (3) are all made of transparent polystyrene material and are made.

6. a kind of Tissue Culture Flask convenient for sampling according to claim 1, which is characterized in that on the lower body (2) Equipped with scale.