CN207672074U - A kind of tumor cell culture device - Google Patents

Abstract

A tumor cell culture device relates to a cell culture device. The oblique neck cap of the culture bottle is equipped with a bottle mouth cap, the bottom of the culture bottle is open, a piston is fixed at one end of the push-pull rod, and a ring-shaped cotton ring is installed in the piston bonding suit, and the ring-shaped cotton ring plug is installed in the culture bottle, and the other end of the push-pull rod The bottom cover of the bottle is fixed, and the center of the rubber block is provided with a round groove for plugging the culture bottle. The rubber block is fixed between two shafts, and the outer ends of the two shafts are respectively rotated in the round holes at the top of the two vertical plates. The lower ends of the two vertical plates are fixed with a base, the first end gear is meshed with the second end gear, the first end gear is fixedly connected to the shaft, the second end gear is slidingly connected to the shaft, and the compression spring is supported between the second end gear and the vertical plate. Each vertical plate is fixed with two position-limiting rod heads, which are respectively spaced and inserted in two position-limiting holes of the corresponding end face gears. The operation is simple, and it is convenient to rotate and position the culture bottle, which is convenient and quick.

Description

A tumor cell culture device

technical field

The utility model relates to a cell culture device, in particular to a tumor cell culture device.

Background technique

The tissue block culture method is a common primary culture method that is simple and easy to implement and has a high success rate. The tissue block culture method is also often used for tumor cell culture. When operating, first drop an appropriate amount of rat tail collagen into the inner wall of the culture bottle and spread it evenly. Form a thin layer of collagen, then transfer the small pieces of tumor tissue into a culture bottle, and add culture medium.

However, due to the torticollis of the current culture bottle, it is not convenient for the small pieces of the tumor tissue to be cut into, and there will be an interval between the small pieces of the tumor tissue, which will affect the operation even more. Similarly, the thin layer of collagen It is also very troublesome to apply. In addition, in the operation engineering, it is necessary to rotate the culture bottle repeatedly. At present, the positioning of the culture bottle usually uses a screw-fastened fixture. When rotating the culture bottle, the screw needs to be loosened, and when the culture bottle is positioned, the screw needs to be tightened, which is inconvenient to use.

Contents of the invention

The purpose of this utility model is to provide a kind of tumor cell culture device, its bottom of the culture bottle is open, and the push-pull rod is provided with a ring cotton ring and the bottom cover of the bottle, which is easy to operate, and the rotating positioning bracket is convenient for rotating the culture bottle. Convenient.

To achieve the above purpose, the utility model adopts the following technical scheme: a tumor cell culture device, including a culture bottle, the shape of the bottle body of the culture bottle is cylindrical, and the mouth of the culture bottle is fixedly connected with a torticollis, and the oblique neck The outer neck end cap is equipped with a bottle mouth cap, the tumor cell culture device also includes a rotating positioning bracket, the bottom of the culture bottle is open, and there is a push-pull rod corresponding to the bottom of the culture bottle, and a piston is fixed at one end of the push-pull rod , the circumferential surface of the piston is bonded with a ring-shaped cotton ring, the ring-shaped cotton ring is tightly plugged in the culture bottle, the other end of the push-pull rod is fixed with a bottle bottom cover, and the rotating positioning bracket includes a rubber block, a base , two shafts, two vertical plates, two end gears 1, two end gears 2 and two compression springs, the center of the rubber block is provided with a circular groove, and the circular groove is used to plug culture bottles , the two shafts are coaxially arranged horizontally, the rubber block is fixed between the two shafts, the two vertical boards are arranged horizontally side by side, and a round hole is respectively provided on the top of the two vertical boards. The outer ends of the shaft rods are respectively rotated and arranged in the two circular holes, the lower ends of the two vertical plates are fixed with bases, and an end face gear 1 and an end face gear 2 are set in the middle of each shaft rod side wall. The first end gear is meshed with the corresponding second end gear, each end gear one is fixedly connected to the corresponding shaft, each end gear two is slidingly connected to the corresponding shaft, and each shaft side wall is also fitted with a Compression spring, each of the compression springs is supported between the corresponding end gear two and the vertical plate, and two limit rod heads are vertically fixed on the inner surface of each vertical plate, and each end gear two is provided with the The two limit rod heads of each vertical plate correspond to the limit holes one by one, and the outer ends of the two limit rod heads of each vertical plate are respectively spaced and inserted in the two described limit holes of the corresponding end face gears.

Compared with the prior art, the beneficial effects of the utility model are:

1. The bottom of the culture bottle of the present invention is open, which is convenient for placing small pieces of tumor tissue. The push-pull rod is equipped with a ring-shaped cotton ring and a bottle bottom cover, which is convenient for applying a thin layer of collagen and sealing the bottom of the culture bottle. It is simple and practical ;

2. The rotating positioning bracket of the utility model is convenient for rotating and positioning the culture bottle, and only needs to manually rotate the culture bottle to the required angle, which is convenient and quick.

Description of drawings

1 is an axonometric view of the overall structure of the tumor cell culture device of the present invention;

Fig. 2 is the split structure axonometric view of culture bottle of the present utility model;

Fig. 3 is the axonometric view of the disassembled structure of the rotary positioning bracket of the present invention;

FIG. 4 is an enlarged view of part A of FIG. 3 .

Detailed ways

The technical solution in the utility model will be clearly and completely described below in conjunction with the accompanying drawings in the utility model embodiment. Obviously, the described embodiment is only a part of the utility model embodiment, rather than all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Specific embodiment 1: As shown in Figures 1 to 4, the utility model discloses a tumor cell culture device, including a culture bottle 1, the shape of the body of the culture bottle 1 is cylindrical, and the mouth of the culture bottle 1 is fixed Connected with a torticollis 1-1, the outer end cap of the torticollis 1-1 is equipped with a bottle cap 2, the tumor cell culture device also includes a rotating positioning bracket, the bottom of the culture bottle 1 is open, and the culture bottle 1 There is a push-pull rod 3 corresponding to the bottom of the bottle, a piston 3-1 is fixed at one end of the push-pull rod 3, and the circumferential surface of the piston 3-1 is bonded with an annular cotton ring 4, and the annular cotton ring 4 is tightly The plug is installed in the culture bottle 1, and the other end of the push-pull rod 3 is fixed with a bottle bottom cap 5. The rotating positioning bracket includes a rubber block 6, a base 9, two shaft rods 7, two vertical plates 8, and two end gears. One 10, two end face gears two 11 and two compression springs 12, the center of the rubber block 6 is provided with a circular groove 6-1, the circular groove 6-1 is used to plug the culture bottle 1, the The two shafts 7 are set horizontally and coaxially, the rubber block 6 is fixed between the two shafts 7, the two vertical plates 8 are horizontally arranged side by side, and the upper ends of the two vertical plates 8 are respectively provided with a round hole, and the two The outer ends of the shaft rods 7 are respectively rotated and arranged in the two circular holes, the lower ends of the two vertical plates 8 are fixed with a base 9, and the middle of each shaft rod 7 side walls is fitted with an end gear 10 and an end gear 2 11. Each of the first face gears 10 is meshed with the corresponding second face gear 11, and each face gear one 10 is fixedly connected to the corresponding shaft 7, and each face gear two 11 is connected to the corresponding shaft 7 Slidingly connected, each side wall of the shaft rod 7 is also sleeved with a compression spring 12, each of the compression springs 12 is supported between the corresponding end gear 2 11 and the vertical plate 8, and the inner surface of each vertical plate 8 is vertical. Two limit rod heads 8-1 are fixed, and each end face gear 2 11 is provided with a limit hole 11-1 corresponding to the two limit rod heads 8-1 one by one, and each vertical plate 8 The outer ends of the two limit rod heads 8-1 are respectively inserted in the two limit holes 11-1 corresponding to the two gears 11 at intervals.

Specific embodiment two: as shown in Fig. 1, 2, this embodiment is the further explanation that specific embodiment one is made, and described culture bottle 1 bottle body outer wall bottom is shaped on external thread 1-2, and described bottle bottom The cap 5 is formed with an internal thread 5-1, and the bottle bottom cap 5 is screwed to the external thread 1-2 of the culture bottle 1 through the internal thread 5-1.

Embodiment 3: As shown in FIG. 1 , this embodiment is a further description of Embodiment 1 or Embodiment 2. The height of the two vertical plates 8 is greater than the length of the culture bottle 1 .

Embodiment 4: As shown in Figures 1 and 3, this embodiment is a further description of Embodiment 1. The lower surface of the base 9 is fixed with a suction cup.

When using the utility model, pull out the push-pull rod 3 and drop an appropriate amount of rat tail collagen in the culture bottle 1, then plug back the ring-shaped cotton ring 4 and push and pull the rotating push-pull rod 3, and use the ring-shaped cotton ring 4 to evenly smear the rat tail collagen on the The inner wall of the culture bottle 1, and then place the small pieces of tumor tissue cut into the culture bottle 1 at intervals from the bottom of the culture bottle 1 (it does not need to be placed from the bottle mouth cap 2, to avoid the inconvenience caused by the torticollis 1-1 to the operation), And the bottle bottom cover 5 is screwed to the bottom of the culture bottle 1. After a certain period of time, the bottle mouth cover 2 is opened to add culture solution to the culture bottle 1. The rotating positioning bracket is convenient for rotating and positioning the culture bottle 1 during operation. Just rotate the culture bottle 1 to the required angle. The culture bottle 1 drives the two shafts 7 to rotate through the rubber block 6, and the two shafts 7 drive the corresponding end gear 10 to rotate. When the end gear 10 rotates, the corresponding The end gear 2 11 vibrates on the side wall of the shaft 7 through the compression spring 12, so that the end gear 2 11 can keep meshing with the end gear 10. When the culture bottle 1 rotates to the required angle, the end gear 12 is under the action of the compression spring 12. The second 11 restricts the rotation of the end gear one 10, thereby positioning the culture bottle 1, and the operation is simple and convenient.

For those skilled in the art, it is obvious that the utility model is not limited to the details of the above-mentioned exemplary embodiments, and that the utility model can be implemented in other forms without departing from the spirit or basic features of the utility model. Therefore, no matter from all points of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, so it is intended to fall within the scope of the claims All changes within the meaning and range of equivalents to the claims are included in the present invention. Any reference sign in a claim should not be construed as limiting the claim concerned.

In addition, it should be understood that although this specification is described according to implementation modes, not each implementation mode only includes an independent technical solution, and this description in the specification is only for clarity, and those skilled in the art should take the specification as a whole , the technical solutions in the various embodiments can also be properly combined to form other implementations that can be understood by those skilled in the art.

Claims (4)

1. A tumor cell culture device, comprising a culture bottle (1), the shape of the body of the culture bottle (1) is cylindrical, and the mouth of the culture bottle (1) is fixed and communicated with a torticollis (1-1). The outer end cap of the torticollis (1-1) described above is equipped with a bottle cap (2), which is characterized in that: the tumor cell culture device also includes a rotating positioning bracket, the bottom of the culture bottle (1) is open, and the culture There is a push-pull rod (3) corresponding to the bottom of the bottle (1). The piston (3-1) is fixed at one end of the push-pull rod (3). ring (4), the ring-shaped cotton ring (4) is tightly plugged in the culture bottle (1), the other end of the push-pull rod (3) is fixed with a bottle bottom cover (5), and the rotating positioning bracket includes a rubber block (6), base (9), two shafts (7), two vertical plates (8), two end gears one (10), two end gears two (11) and two compression springs (12) , the center of the rubber block (6) is provided with a round slot (6-1), the round slot (6-1) is used to plug the culture bottle (1), and the two shafts (7) The horizontal coaxial arrangement, the rubber block (6) is fixed between the two shafts (7), the two vertical plates (8) are arranged horizontally side by side, and the upper ends of the two vertical plates (8) are respectively provided with a circle The outer ends of the two shafts (7) are respectively rotated and set in the two circular holes, the lower ends of the two vertical plates (8) are fixed with a base (9), and the middle of the side wall of each shaft (7) is set There is a face gear 1 (10) and a face gear 2 (11), each said face gear 1 (10) is meshed with the corresponding said face gear 2 (11), and each face gear 1 (10) Fixedly connected with the corresponding shaft (7), each end face gear two (11) is slidingly connected with the corresponding shaft (7), and each shaft (7) side wall is also equipped with a compression spring (12), each The compression spring (12) mentioned above is supported between the corresponding end face gear 2 (11) and the vertical plate (8), and two limit rod heads (8- 1), each end face gear 2 (11) is provided with a limit hole (11-1) corresponding to the two limit rod heads (8-1) one by one, and each vertical plate (8) two The outer ends of the root limiting rod heads (8-1) are respectively spaced and inserted in the two aforementioned limiting holes (11-1) of the corresponding end face gear two (11). 2. A tumor cell culture device according to claim 1, characterized in that: the bottom of the outer wall of the culture bottle (1) is made with external threads (1-2), and the bottom cover of the bottle (5 ) is made with an internal thread (5-1), and the bottle bottom cap (5) is screwed to the external thread (1-2) of the culture bottle (1) through the internal thread (5-1). 3. A tumor cell culture device according to claim 1 or 2, characterized in that: the height of the two vertical plates (8) is greater than the length of the culture bottle (1). 4. A tumor cell culture device according to claim 1, characterized in that: a suction cup is fixed on the lower surface of the base (9).