CN207091416U - A kind of western hybridization beaker for experiment - Google Patents

Abstract

The utility model provides a beaker for protein hybridization experiment, which comprises a sealing cover plate, a stirring blade, a rotating shaft, a hollow pin, a sealing plug, a storage bottle, a connecting pipe, a hand-held air cylinder and a hose. The stirring blade is installed on the rotating shaft On the shaft, there is a sealing cover plate, and a stirring blade is installed under the sealing cover plate. This design realizes the stirring function and reduces the reaction time. The sealing plug is installed on the hollow pin, and the right end of the hollow pin There is a hose, the right end of the hose is provided with a hand-held gas cylinder, the lower end of the hose is equipped with a connecting pipe, the lower end of the connecting pipe is provided with a storage bottle, and the storage bottle is connected to the hose through the connecting pipe , the design can add materials in a sealed state, and at the same time reduce equipment costs. The utility model is easy to use, increases hybridization efficiency, improves product quality, and has high reliability.

Description

A beaker for protein hybridization experiment

technical field

The utility model relates to a beaker for protein hybridization experiments, which belongs to the technical field of protein hybridization experiments.

Background technique

Protein hybridization is yeast two-hybrid, which is a genetic system for analyzing protein-protein interactions in yeast and a genetic method based on the modular structure of transcription factors. Since the establishment of this method, through continuous improvement and development, it can not only detect the interaction between known proteins, but more importantly, discover new unknown proteins that interact with known proteins.

When the existing experimental beakers are used for protein hybridization, the hybridization reaction is often carried out naturally, resulting in a long hybridization time and increasing the hybridization efficiency. When adding materials to the existing experimental beakers, the materials are often directly put into the beaker from outside. As a result, the material is in contact with the outside air, which will pollute the material and reduce the product quality.

Utility model content

In view of the deficiencies in the prior art, the purpose of this utility model is to provide a beaker for protein hybridization experiments to solve the problems raised in the above-mentioned background technology. The utility model is easy to use, increases hybridization efficiency, improves product quality, and has high reliability .

In order to achieve the above object, the utility model is achieved through the following technical solutions: a beaker for protein hybridization experiments, including a stirring mechanism, a beaker main body and a feeding mechanism, the stirring mechanism is installed on the beaker main body, the right end of the beaker main body A feeding mechanism is provided, and the stirring mechanism includes a sealing cover plate, a stirring blade and a rotating shaft. The sealing cover plate is arranged on the upper end of the beaker main body, the stirring blade and the rotating shaft are installed inside the beaker main body, and the feeding mechanism includes a hollow pin, a sealing plug, a storage bottle, a connecting pipe, a hand-held air cylinder and a hose. The sealing plug is installed on the hollow pin, the right end of the hollow pin is provided with a hose, the right end of the hose is provided with a hand-held air cylinder, the lower end of the hose is installed with a connecting pipe, and the lower end of the connecting pipe is provided with a storage The feed bottle is connected to the hose through a connecting pipe, and the sealing plug is installed at the right end of the beaker main body.

Further, the sealing cover plate is connected to the beaker main body through threads, and a sealing ring is installed at the connection position between the sealing cover plate and the beaker main body.

Further, there are at least two stirring blades, at least two stirring blades are evenly installed on the rotating shaft, a handle is provided at the left end of the rotating shaft, and the handle is installed above the sealing cover plate, and the sealing cover plate and the rotating shaft connected by bearings.

Further, the right end of the main body of the beaker is provided with a feeding tube, and a sealing plug is installed on the feeding tube, and the left end of the hollow pin extends into the feeding tube.

Further, there are two storage bottles and connecting pipes, and the connecting pipes are installed at the lower end of the hose. Protein solution and solid-phase support liquid are respectively installed in the two storage bottles. On-off valves and one-way valves are installed on the connecting pipes.

Further, a sponge sleeve is installed on the handheld air cylinder.

Beneficial effects of the utility model: a beaker for protein hybridization experiments. The utility model realizes the stirring function by adding a sealing cover plate, a stirring blade and a rotating shaft, reduces reaction time, increases hybridization efficiency, and solves the problem of existing experimental beakers. When performing protein hybridization, the hybridization reaction is often carried out naturally, resulting in long hybridization time and increased hybridization efficiency.

Due to the addition of hollow pins, sealing plugs, storage bottles, connecting pipes, hand-held gas cylinders and hoses, this design can add materials in a sealed state, improve product quality, and reduce equipment costs at the same time, solving the problem of existing experimental beakers. When adding materials, the materials are often put into the beaker directly from the outside, causing the materials to contact with the outside air, which will pollute the materials and reduce product quality.

Because of the added thread, this design is easy to disassemble the sealing cover plate, because of the added sealing ring, this design improves the sealing effect, because of the added handle, this design can rotate the shaft, and because of the added feeding tube, this design is not only convenient for adding materials, but also easy to discharge The product, because of the addition of on-off valve and one-way valve, this design realizes the feeding of different materials, because of the addition of sponge cover, this design improves the comfort of personnel, the utility model is easy to use, increases the efficiency of hybridization, improves product quality, and has high reliability.

Description of drawings

Other features, objects and advantages of the present invention will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings:

Fig. 1 is the structural representation of a kind of protein hybridization experiment beaker of the present utility model;

Fig. 2 is the schematic diagram of a stirring mechanism in a beaker for a protein hybridization experiment of the present invention;

Fig. 3 is the schematic diagram of feeding mechanism in a kind of protein hybridization experiment beaker of the present utility model;

In the figure: 1-stirring mechanism, 2-beaker main body, 3-feeding mechanism, 4-sealing cover plate, 5-stirring blade, 6-rotating shaft, 7-hollow pin, 8-sealing plug, 9-storage bottle, 10-connecting pipe, 11-hand-held gas cylinder, 12-flexible hose.

Detailed ways

In order to make the technical means, creative features, goals and effects achieved by the utility model easy to understand, the utility model will be further elaborated below in conjunction with specific embodiments.

Please refer to Fig. 1-Fig. 3, the utility model provides a kind of technical scheme: a kind of beaker for protein hybridization experiment, comprises agitating mechanism 1, beaker main body 2 and feeding mechanism 3, agitating mechanism 1 is installed on beaker main body 2, beaker main body 2 The right end is provided with feeding mechanism 3.

The stirring mechanism 1 includes a sealing cover 4, a stirring blade 5 and a rotating shaft 6, the stirring blade 5 is installed on the rotating shaft 6, the rotating shaft 6 is provided with a sealing cover 4, the stirring blade 5 is installed under the sealing cover 4, and the sealing cover 4 It is arranged on the upper end of the beaker main body 2 , and the stirring blade 5 and the rotating shaft 6 are installed inside the beaker main body 2 .

The feeding mechanism 3 includes a hollow pin 7, a sealing plug 8, a storage bottle 9, a connecting pipe 10, a hand-held gas cylinder 11 and a hose 12, the sealing plug 8 is installed on the hollow pin 7, and the right end of the hollow pin 7 is provided with a soft Pipe 12, the right end of the flexible pipe 12 is provided with a hand-held gas cylinder 11, the lower end of the flexible pipe 12 is equipped with a connecting pipe 10, the lower end of the connecting pipe 10 is provided with a storage bottle 9, and the storage bottle 9 is connected with the flexible pipe 12 through the connecting pipe 10, The sealing plug 8 is installed on the right end of the beaker main body 2 .

The sealing cover plate 4 is connected with the beaker main body 2 by threads, and a sealing ring is installed at the connection position between the sealing cover plate 4 and the beaker main body 2. There are at least two stirring blades 5, and at least two stirring blades 5 are evenly installed on the rotating shaft 6. The left end of the rotating shaft 6 is provided with a handle, and the handle is installed above the sealing cover plate 4, the sealing cover plate 4 is connected with the rotating shaft 6 through a bearing, the right end of the beaker main body 2 is provided with a feed pipe, and a sealing plug 8 is installed on the feed pipe, The left end of the hollow pin 7 extends into the feeding pipe. There are two storage bottles 9 and connecting pipes 10. The connecting pipes 10 are installed at the lower end of the flexible pipe 12. Protein solution and solids are respectively installed in the two storage bottles 9. To support the liquid, on-off valves and one-way valves are installed on the two connecting pipes 10, and a sponge sleeve is installed on the hand-held gas cylinder 11.

Specific implementation method: when using, the user first checks the utility model to see if there is a defect. If there is a defect, it cannot be used. At this time, the maintenance personnel need to be notified to carry out maintenance. If there is no problem, it can be carried out. In use, before use, the user first sterilizes the inside of the beaker main body 2, then installs the sealing plug 8 on the feed pipe, then inserts the hollow pin 7 through the sealing plug 8, and then opens the beaker that is installed with the solid-phase support liquid. The on-off valve on the connection pipe 10 at the upper end of the storage bottle 9, and then the user first uses the hand-held gas cylinder 11 to draw the solid-phase support liquid in the storage bottle 9 into the hose 12 through the connection pipe 10, and then through the hollow plug The needle 7 is squeezed into the beaker main body 2. After the completion, the user closes the on-off valve, then opens the on-off valve on another connecting pipe 10, and then uses the hand-held air cylinder 11 to put the protein solution into the beaker main body 2. The design can add materials in a sealed state, improving product quality and reducing equipment costs.

After the feeding is completed, the user holds the handle and turns it. The rotation of the handle drives the rotation of the rotating shaft 6, and the rotation of the rotating shaft 6 drives the rotation of the stirring blade 5 to realize the stirring of the mixed solution in the beaker main body 2. This design solves the problem of the existing experimental beaker. When proteins are hybridized, the hybridization reaction is often carried out naturally, resulting in long hybridization time and increased hybridization efficiency.

The basic principles and main features of the utility model and the advantages of the utility model have been shown and described above. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above-mentioned exemplary embodiments, and without departing from the utility model The present utility model can be realized in other specific forms without the spirit or essential features. Therefore, no matter from all points of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, so it is intended to fall within the scope of the claims All changes within the meaning and range of equivalents of the required elements are included in the present invention. Any reference sign in a claim should not be construed as limiting the claim concerned.

In addition, it should be understood that although this specification is described according to implementation modes, not each implementation mode only contains an independent technical solution, and this description in the specification is only for clarity, and those skilled in the art should take the specification as a whole , the technical solutions in the various embodiments can also be properly combined to form other implementations that can be understood by those skilled in the art.

Claims (6)

1. a kind of western hybridization beaker for experiment, including rabbling mechanism (1), beaker main body (2) and batch charging mechanism (3), its It is characterised by：The rabbling mechanism (1) is arranged in beaker main body (2), and beaker main body (2) right-hand member is provided with batch charging mechanism (3)；

The rabbling mechanism (1) includes seal cover board (4), paddle (5) and rotating shaft (6), and the paddle (5), which is arranged on, to be turned On axle (6), the rotating shaft (6) is provided with seal cover board (4), and paddle (5) is provided with below the seal cover board (4), described Seal cover board (4) is arranged on beaker main body (2) upper end, and the paddle (5) and rotating shaft (6) are installed in beaker main body (2) It is internal；

The batch charging mechanism (3) includes hollow contact pin (7), sealing-plug (8), material stock bottle (9), connecting tube (10), hand-held inflator (11) and flexible pipe (12), the sealing-plug (8) are arranged on hollow contact pin (7), and hollow contact pin (7) right-hand member is provided with flexible pipe (12), flexible pipe (12) right-hand member is provided with hand-held inflator (11), and flexible pipe (12) lower end is provided with connecting tube (10), institute State connecting tube (10) lower end and be provided with material stock bottle (9), the material stock bottle (9) is connected by connecting tube (10) with flexible pipe (12), institute State sealing-plug (8) and be arranged on beaker main body (2) right-hand member.

A kind of 2. western hybridization beaker for experiment according to claim 1, it is characterised in that：The seal cover board (4) It is connected by screw thread with beaker main body (2), the seal cover board (4) is provided with sealing ring with beaker main body (2) link position.

A kind of 3. western hybridization beaker for experiment according to claim 1, it is characterised in that：The paddle (5) sets Having at least two, at least two paddles (5) are uniformly arranged in rotating shaft (6), and rotating shaft (6) left end is provided with handle, And handle is arranged on above seal cover board (4), the seal cover board (4) is connected with rotating shaft (6) by bearing.

A kind of 4. western hybridization beaker for experiment according to claim 1, it is characterised in that：The beaker main body (2) Right-hand member is provided with feed pipe, and sealing-plug (8) is provided with feed pipe, and hollow contact pin (7) left end is extended into feed pipe.

A kind of 5. western hybridization beaker for experiment according to claim 1, it is characterised in that：The material stock bottle (9) with And connecting tube (10) is equipped with two, the connecting tube (10) is installed in flexible pipe (12) lower end, and two material stock bottles (9) are interior It is separately installed with protein solution and solid phase supports liquid, switch valve and list is mounted on two connecting tubes (10) To valve.

A kind of 6. western hybridization beaker for experiment according to claim 1, it is characterised in that：The hand-held inflator (11) sponges cover is installed on.