CN206872807U - PCR low temperature sample adding device - Google Patents
PCR low temperature sample adding device Download PDFInfo
- Publication number
- CN206872807U CN206872807U CN201720789317.7U CN201720789317U CN206872807U CN 206872807 U CN206872807 U CN 206872807U CN 201720789317 U CN201720789317 U CN 201720789317U CN 206872807 U CN206872807 U CN 206872807U
- Authority
- CN
- China
- Prior art keywords
- ice chest
- low temperature
- pcr
- adding device
- sample adding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
A kind of PCR low temperature sample adding device, it is characterized in that it is mainly by ice chest（1）Dividing plate is placed with PCR plate（2）Composition, described PCR plate place dividing plate（2）From ice chest（1）Opening（3）Place is placed on its ice chest inner bottom surface, described ice chest（1）Box body be double-decker, low temperature energy-accumulation material is marked with inside it, to ensure its cryogenic property.The utility model is simple in construction, manufactures, be easy to use, can improve the accuracy and efficiency for mending experiment, the low temperature environment needed for guarantee test.When carrying out qPCR experiments, additional steps can be completed in 4 DEG C of environment temperature.
Description
Technical field
A kind of experimental provision is the utility model is related to, especially a kind of Medical low-temperature experiment apparatus is specifically a kind of
PCR low temperature sample adding device.
Background technology
It is well known that PCR（PCR）It is a kind of common experimental method, it is specific for amplifying amplification
DNA fragmentation.PCR plate（Including 96 orifice plates and 384 orifice plates）It is important experiment equipment in qPCR experiments, will be to be detected during experiment
Article, which is added in PCR plate, is used for subsequent survey, and this whole experiment process is both needed to carry out in 4 DEG C of environment as far as possible, to protect
The stability of sample and reagent is demonstrate,proved, so that experimental result is more accurate.At present when carrying out this experiment, most of steps
It can ensure to operate on ice, meet 4 DEG C of environment temperature as far as possible, but when being loaded into PCR plate, such as PCR plate is placed
On ice, then it can make it that PCR plate is unstable, influences to be loaded；Water or ice cube can be stained with PCR plate, influences testing result.Therefore,
PCR plate during being loaded how is kept to maintain as far as possible in 4 DEG C of environment, and do not influence experimental implementation and experimental result to become
One it is to be solved the problem of.
Utility model content
The purpose of this utility model is to be directed to existing PCR（PCR）During, PCR plate places inconvenience
Problem, design a kind of convenient and reliable PCR low temperature sample adding device of placement.
The technical solution of the utility model is：
A kind of PCR low temperature sample adding device, it is characterized in that it mainly places dividing plate by ice chest 1 and PCR plate
2 compositions, described PCR plate are placed dividing plate 2 and are placed at the opening 3 of ice chest 1 on its ice chest inner bottom surface, described ice chest 1
Box body is double-decker, low temperature energy-accumulation material is marked with inside it, to ensure its cryogenic property.Described low temperature energy-accumulation material is solidifying
Glue, main component are water, sugar and sodium carboxymethylcellulose.
Described ice chest 1 is provided with least one energy-accumulation material inlet 4.
Slipmat 5 is added on the outer bottom of described ice chest 1.
The beneficial effects of the utility model：
The utility model need not install ice cube additional, can guarantee that the level of PCR plate, prevent sample from sliding.
PCR plate of the present utility model is placed dividing plate and is made up of Hydrophobic glass, in ice chest, on the one hand because its is hydrophobic
Property, can avoid liquefying caused by the globule stick in PCR plate, on the other hand because color is transparent, be easy to observe sample-adding situation, its
Also will not be by bursting by freezing at -20 DEG C.
The utility model is simple in construction, manufactures, be easy to use, can improve the accuracy and efficiency for mending experiment, guarantee test
Required low temperature environment.When carrying out qPCR experiments, additional steps can be completed in 4 DEG C of environment temperature.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
The utility model is further described with reference to the accompanying drawings and examples.
As shown in Figure 1.
A kind of PCR low temperature sample adding device, it mainly places dividing plate 2 by ice chest 1 and PCR plate and formed, institute
The PCR plate stated places dividing plate 2 and places or be inlaid on the inner bottom surface of ice chest 1 at the opening 3 of ice chest 1, the overall concave knot of ice chest
Structure, the box body of described ice chest 1 is double-decker, low temperature energy-accumulation material is marked with inside it, to ensure its cryogenic property.Described
Low temperature energy-accumulation material is gel, and main component is water, sugar and sodium carboxymethylcellulose.At least one storage is provided with ice chest 1
Energy material inlet 4, while slipmat 5 is added on the outer bottom of ice chest 1, as shown in Figure 1.PCR plate places dividing plate 2 can be by
Hydrophobic glass is made, in ice chest, on the one hand due to its hydrophobicity, can avoid liquefying caused by the globule stick in PCR plate,
On the other hand because color is transparent, it is easy to observe sample-adding situation, it also will not be by bursting by freezing at -20 DEG C.
Usually ice chest 1 is placed in -20 DEG C of refrigerators, the energy-accumulation material in its interlayer can at low temperature absorb and store big
Measure cold；When it is desired to be used, PCR plate is placed in into PCR plate to place on dividing plate 2, energy-accumulation material can discharge a large amount of colds, maintain
PCR plate is in relatively low temperature.
The utility model is not directed to partly same as the prior art or can realized using prior art.
Claims (3)
- A kind of 1. PCR low temperature sample adding device, it is characterized in that it is mainly by ice chest（1）Dividing plate is placed with PCR plate （2）Composition, described PCR plate place dividing plate（2）From ice chest（1）Opening（3）Place is placed on its ice chest inner bottom surface, described Ice chest（1）Box body be double-decker, low temperature energy-accumulation material is marked with inside it, to ensure its cryogenic property.
- 2. PCR according to claim 1 low temperature sample adding device, it is characterized in that described ice chest（1）On Provided with least one energy-accumulation material inlet（4）.
- 3. PCR according to claim 1 low temperature sample adding device, it is characterized in that described ice chest（1）'s Slipmat is added on outer bottom（5）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720789317.7U CN206872807U (en) | 2017-07-03 | 2017-07-03 | PCR low temperature sample adding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720789317.7U CN206872807U (en) | 2017-07-03 | 2017-07-03 | PCR low temperature sample adding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206872807U true CN206872807U (en) | 2018-01-12 |
Family
ID=61330746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720789317.7U Expired - Fee Related CN206872807U (en) | 2017-07-03 | 2017-07-03 | PCR low temperature sample adding device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206872807U (en) |
-
2017
- 2017-07-03 CN CN201720789317.7U patent/CN206872807U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180112 Termination date: 20180703 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |