CN206607234U - A kind of cell culture liquid-transfering device - Google Patents

A kind of cell culture liquid-transfering device Download PDF

Info

Publication number
CN206607234U
CN206607234U CN201720292602.8U CN201720292602U CN206607234U CN 206607234 U CN206607234 U CN 206607234U CN 201720292602 U CN201720292602 U CN 201720292602U CN 206607234 U CN206607234 U CN 206607234U
Authority
CN
China
Prior art keywords
straight tube
liquid
piston
liquid storage
transfering
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201720292602.8U
Other languages
Chinese (zh)
Inventor
张炳强
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Re-Store Biotech Co Ltd
Original Assignee
Qingdao Re-Store Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Re-Store Biotech Co Ltd filed Critical Qingdao Re-Store Biotech Co Ltd
Priority to CN201720292602.8U priority Critical patent/CN206607234U/en
Application granted granted Critical
Publication of CN206607234U publication Critical patent/CN206607234U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The utility model discloses a kind of cell culture liquid-transfering device, including straight tube body, bent tubular body, piston cylinder and liquid storage cylinder;The external side of straight tube below fixed seat, fixed seat is fixed with the outside of the straight tube body and is provided with a circle seal;Nuclepore membrane filter is installed, nuclepore membrane filter is communicated with gas via-hole in the fixed seat;Check valve is installed, the upper end connection bent tubular body of straight tube body is communicated with liquid storage cylinder in bent tubular body other end, air-vent is offered on the side wall of liquid storage cylinder, air-vent is provided with check valve in the straight tube body;Piston is installed, piston upper end is fixedly mounted the body of rod, handle is fixed with the end of the body of rod in piston cylinder.The utility model is simple in construction, easy to operate, and different bottlenecks are can adapt to by seal, it is to avoid with contacting external air, protect internal liquid, piston can pull multiple, the enough liquid of extraction up and down, it is ensured that examine sufficient with liquid, it is to avoid multi-pass operation.

Description

A kind of cell culture liquid-transfering device
Technical field
The utility model is related to cell therapy field, specifically a kind of cell culture liquid-transfering device.
Background technology
Carry out, and needed during culture to culture frequently with Tissue Culture Flask, culture bag etc. in cell culture Liquid is detected in real time, real to obtain cell density in incubation, the acid-base value of nutrient solution, induction or metabolite concentration etc. When status information.
It is typically to open the Tissue Culture Flask or culture bag cultivated based on the above-mentioned mode detected in real time, Then the nutrient solution of certain volume is suctioned out with pipette, then the nutrient solution of absorption is carried out to the detection of above-mentioned indices. Outside above-mentioned sampling operation, also often need to carry out the sample-adding operation of fresh medium, induction stimulating factor etc..In the training of whole cell In foster process, the number of times of sampling and sample-adding detection is very more, endlessly opens Tissue Culture Flask open container mouthful, therefore When container open is sampled and be loaded operation with pipette, the living contaminants deepened significantly in cell cultivation process may; Existing a kind of pipette, although a small amount of nutrient solution can be drawn by piston, it is impossible to reflect whole positions in blake bottle Situation analyzed, it is necessary to extract more nutrient solution.
Utility model content
The purpose of this utility model is to provide a kind of cell culture liquid-transfering device, to solve to carry in above-mentioned background technology The problem of going out.
To achieve the above object, the utility model provides following technical scheme:
A kind of cell culture liquid-transfering device, including straight tube body, bent tubular body, piston cylinder and liquid storage cylinder;The straight tube body Outside is fixed with fixed seat, and fixed seat is that the external side of straight tube below platy structure, fixed seat is provided with a circle seal, sealing The section of body is semicircle, and seal is looped around the outside of straight tube body;Nuclepore membrane filter is installed in the fixed seat, it is micro- Hole membrane filter is communicated with gas via-hole, and gas via-hole extends downwardly into the lower section of seal;It is provided with the straight tube body Check valve, the upper end connection bent tubular body of straight tube body, is communicated with liquid storage cylinder, under the bottom of liquid storage cylinder in bent tubular body other end End is communicated with drain pipe, and air-vent is offered on the side wall of liquid storage cylinder, and air-vent is provided with check valve;The top of the liquid storage cylinder Portion, which is communicated with piston cylinder, piston cylinder, is provided with piston, and the body of rod is fixedly mounted in piston upper end, and the body of rod passes piston cylinder, in the body of rod End on be fixed with handle.
Further:The material of the seal is rubber.
Further:The bent tubular body is inverted U-shaped structure.
Further:The bottom of the liquid storage cylinder is set to incline structure.
Further:Valve is installed on the drain pipe.
Compared with prior art, the beneficial effects of the utility model are:The utility model is simple in construction, easy to operate, leads to Different bottlenecks can be adapted to by crossing sealing body, it is to avoid with contacting external air, protect internal liquid, piston can pull many up and down It is secondary, extract enough liquid, it is ensured that examine sufficient with liquid, it is to avoid multi-pass operation.
Brief description of the drawings
Fig. 1 is a kind of structural representation of cell culture liquid-transfering device.
In figure:1- straight tube bodies, 2- bent tubular bodies, 3- piston cylinders, 4- liquid storage cylinders, 5- seals, 6- fixed seats, 7- miillpore filters Filter, 8- gas via-holes, 9- check valves, 10- pistons, the 11- bodies of rod, 12- handles, 13- air-vents, 14- drain pipes.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of the utility model, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made The every other embodiment obtained, belongs to the scope of the utility model protection.
Refer in figure, the utility model embodiment, a kind of cell culture liquid-transfering device, including straight tube body 1, bent tubular body 2nd, piston cylinder 3 and liquid storage cylinder 4;The outside of the straight tube body 1 is fixed with fixed seat 6, under fixed seat 6 is platy structure, fixed seat 6 The outside of straight tube body 1 of side is provided with a circle seal 5, and the section of seal 5 is semicircle, and seal 5 is looped around straight tube body 1 Outside, its material is rubber, can be sealed the opening of blake bottle etc. by seal 5, seal 5 can adapt to different-diameter Opening, it is ensured that most opening can be sealed, strong adaptability;Filtering with microporous membrane is installed in the fixed seat 6 Device 7, nuclepore membrane filter 7 is communicated with gas via-hole 8, and gas via-hole 8 extends downwardly into the lower section of seal 5;The straight tube Check valve 9 is installed in body 1, the liquid in straight tube body 1 is flowed up, the upper end connection bent tubular body 2 of straight tube body 1 is curved Body 2 is inverted U-shaped structure, and liquid storage cylinder 4 is communicated with the other end of bent tubular body 2, and the bottom of liquid storage cylinder 4 is set to tilt Structure, is easy to, by the liquid discharge in liquid storage cylinder 4, be communicated with drain pipe 14, drain pipe 14 and pacify in the bottom lower end of liquid storage cylinder 4 Equipped with valve, liquid taking is convenient, and air-vent 13 is offered on the side wall of liquid storage cylinder 4, and air-vent 13 is provided with check valve 9, Discharge the air of inside;The top of the liquid storage cylinder 4 is communicated with piston cylinder 3, piston cylinder 3 and is provided with piston 10, living Fill in 10 upper ends and the body of rod 11 is fixedly mounted, the body of rod 11 passes piston cylinder 3, handle 12 is fixed with the end of the body of rod 11, passes through hand The dynamic push-and-pull body of rod 11, realizes that piston 10 is moved.
In use, straight tube body 1 is inserted into blake bottle, opening is sealed by the seal 5 of lower end, it is to avoid due to pipe Bore dia is different and outside air is entered inside, protects internal liquid;Manual-up pull handle 12, makes piston 10 upward It is mobile, nutrient solution is drawn into liquid storage cylinder 4, pushing down on piston 10, due to check valve, make internal gas from air-vent 13 discharges, enable piston 10 to aspirate again up, draw liquid into the liquid in liquid storage cylinder 4 in collection, liquid storage cylinder 4 and pass through Drain pipe 14 is discharged;The utility model is simple in construction, easy to operate, and different bottlenecks are can adapt to by seal, it is to avoid with Contacting external air, protects internal liquid, and piston can pull multiple, the enough liquid of extraction, it is ensured that inspection liquid up and down Body is sufficient, it is to avoid multi-pass operation.
It is obvious to a person skilled in the art that the utility model is not limited to the details of above-mentioned one exemplary embodiment, and And in the case of without departing substantially from spirit or essential attributes of the present utility model, can realize that this practicality is new in other specific forms Type.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this practicality is new The scope of type limits by appended claims rather than described above, it is intended that the equivalency fallen in claim is contained All changes in justice and scope are included in the utility model.Any reference in claim should not be considered as limitation Involved claim.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art It may be appreciated other embodiment.

Claims (5)

1. a kind of cell culture liquid-transfering device, including straight tube body(1), bent tubular body(2), piston cylinder(3)And liquid storage cylinder(4);Its It is characterised by:The straight tube body(1)Outside be fixed with fixed seat(6), fixed seat(6)For platy structure, fixed seat(6)Lower section Straight tube body(1)Outside is provided with a circle seal(5), seal(5)Section for semicircle, seal(5)It is looped around straight Body(1)Outside;The fixed seat(6)On nuclepore membrane filter is installed(7), nuclepore membrane filter(7)It is communicated with Gas via-hole(8), gas via-hole(8)Extend downwardly into seal(5)Lower section;The straight tube body(1)Check valve is inside installed (9), straight tube body(1)Upper end connection bent tubular body(2), in bent tubular body(2)Liquid storage cylinder is communicated with other end(4), in liquid storage Chamber(4)Bottom lower end be communicated with drain pipe(14), in liquid storage cylinder(4)Side wall on offer air-vent(13), air-vent (13)It is provided with check valve(9);The liquid storage cylinder(4)Top be communicated with piston cylinder(3), piston cylinder(3)Piston is inside installed (10), piston(10)The body of rod is fixedly mounted in upper end(11), the body of rod(11)Pass piston cylinder(3), in the body of rod(11)End on it is solid Surely there is handle(12).
2. a kind of cell culture liquid-transfering device according to claim 1, it is characterised in that:The seal(5)Material Matter is rubber.
3. a kind of cell culture liquid-transfering device according to claim 1, it is characterised in that:The bent tubular body(2)To fall The U-shaped structure put.
4. a kind of cell culture liquid-transfering device according to claim 1, it is characterised in that:The liquid storage cylinder(4)Bottom Portion is set to incline structure.
5. a kind of cell culture liquid-transfering device according to claim 1, it is characterised in that:The drain pipe(14)Upper peace Equipped with valve.
CN201720292602.8U 2017-03-24 2017-03-24 A kind of cell culture liquid-transfering device Active CN206607234U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720292602.8U CN206607234U (en) 2017-03-24 2017-03-24 A kind of cell culture liquid-transfering device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720292602.8U CN206607234U (en) 2017-03-24 2017-03-24 A kind of cell culture liquid-transfering device

Publications (1)

Publication Number Publication Date
CN206607234U true CN206607234U (en) 2017-11-03

Family

ID=60174326

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720292602.8U Active CN206607234U (en) 2017-03-24 2017-03-24 A kind of cell culture liquid-transfering device

Country Status (1)

Country Link
CN (1) CN206607234U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109735453A (en) * 2019-02-26 2019-05-10 陕西师范大学 A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109735453A (en) * 2019-02-26 2019-05-10 陕西师范大学 A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus

Similar Documents

Publication Publication Date Title
CN206607234U (en) A kind of cell culture liquid-transfering device
CN107677512A (en) Earth's surface water isotope, water chemistry layered water sample harvester
CN105967358A (en) Waste liquid and gas collecting device of small underground water seepage physical model
CN103610474B (en) Urine collecting and sampling device
CN208171677U (en) A kind of water quality detection sampler
CN206369647U (en) A kind of strand tidal beach wetland Sediment Pore Water sampler
CN206684134U (en) A kind of detecting system for Food Monitoring early warning
CN206504898U (en) A kind of sewage sampling device
CN206587138U (en) A kind of Nephrology dept.'s waste collecting device
CN206746584U (en) A kind of sampler for Chemical Engineering
CN207123418U (en) A kind of sampler for liquid food sampling Detection
CN205670111U (en) Simulation constant head permeability apparatus
CN206114370U (en) Air sampling instrument
CN204931696U (en) A kind of blood taking needle for medical test
CN105092279B (en) A kind of sample collection device
CN207570859U (en) A kind of collection device of polycyclic aromatic hydrocarbon volatile matter in soil
CN206261671U (en) A kind of cleaning solution automatically adding device of liquid waste collector
CN206960179U (en) A kind of urological department sufferer urine sample collector
CN205774526U (en) A kind of cell cultivation pipet
CN206881734U (en) Remaining waste remove device
CN206440490U (en) Depthkeeping quantitative liquid sampler
CN208902468U (en) A kind of javelle water sampler
CN206095690U (en) Water quality monitoring sampling device
CN206804352U (en) The horizontal portable water sampler of straight neck twoport
CN208500961U (en) A kind of novel pasteur pipet

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant