CN206523348U - Electron microscope ultra-thin section dyeing apparatus and ultra-thin section overflow dyeing machine - Google Patents

Abstract

The utility model provides an electron microscope ultra-thin slice dyeing device and an ultra-thin slice dyeing machine, and relates to the technical field of ultra-thin slice dyeing, wherein the electron microscope ultra-thin slice dyeing device includes a net-carrying device, the net-carrying device includes a support plate, a support Mesh grooves are evenly distributed on the board; the containing device includes a transparent dyeing bag; the water injection assembly includes a water injection pipe connected to the water injection device; the liquid injection assembly includes a first liquid injection pipe and a second liquid injection pipe, and the first liquid injection pipe is connected to a useful The first liquid injection device for injecting uranyl acetate dye solution; the second liquid injection pipe is connected with the second liquid injection device for injecting lead citrate dye solution; the exhaust device includes an exhaust pipe, and the exhaust pipe is connected with an exhaust pipe. The air filter device; the waste liquid output device includes a liquid discharge pipe connected with a liquid storage bag. The electron microscope ultra-thin section dyeing device provided by the utility model not only realizes the dyeing effect, but also effectively avoids the pollution caused by the dyeing solution.

Description

Electron microscope ultra-thin section staining device and ultra-thin section staining machine

technical field

The utility model relates to the technical field of electron microscope ultrathin section dyeing, in particular to an electron microscope ultrathin section dyeing device and an ultrathin section dyer.

Background technique

Electron staining is a method that uses certain metal salts (such as lead, uranium, osmium, etc.) to combine with certain structures and components of cells to increase their electron scattering capabilities, thereby improving contrast. With different numbers of heavy metal atoms, the area with more heavy metal atoms (that is, the part with dense structure and high atomic number) has a strong electron scattering ability, which appears electron-dense black under the electron microscope; the area with less heavy metal atoms is It is light black, gray black, and the area without heavy metals is an electronic transparent area. Therefore, the sample contrast can be improved and the image clarity can be increased after electronic dyeing treatment.

Uranyl acetate staining and lead citrate staining are the most commonly used staining methods in ultrathin section staining of electron microscope samples. Therefore, the corresponding commonly used electronic stains are two staining solutions of uranyl acetate and lead citrate. Among them, Uranyl acetate, also known as uranyl acetate and uranyl acetate, is a widely used staining agent, which is mainly used to improve the contrast of nucleic acid, protein and connective tissue fibers, and has a poor staining effect on membranes; lead citrate is currently used The most extensive staining agent for electron microscopy, with high density, has a wide range of affinity effects on various tissue structures, especially to improve the contrast of cell membrane systems and lipid substances, and has a better staining effect on glycogen that cannot be stained by osmic acid .

In view of the advantages and disadvantages of the two staining solutions of uranyl acetate and lead citrate, that is, uranium and lead have different staining characteristics, so at present, double staining is generally used for ultra-thin section staining: that is, staining with uranyl acetate first Finally, dye with lead citrate to complement each other to obtain better dyeing effect.

However, the uranyl acetate dye solution has certain radioactivity, and if it is not handled properly, it will damage the health of the experimenters and pollute the surrounding environment; the lead citrate dye solution is very easy to combine with carbon dioxide in the air to form Precipitation of lead carbonate particles, lead carbonate particle precipitation will cause contamination of ultra-thin sections, which seriously affects the observation of ultra-thin sections by electron microscopy.

Therefore, ultra-thin section staining lacks a reasonable use of devices for protection, and improvement is urgently needed.

Utility model content

The purpose of the utility model is to provide an electron microscope ultra-thin section dyeing device and an ultra-thin section dyeing machine, wherein the electron microscope ultra-thin section dyeing device not only realizes the dyeing effect, but also effectively avoids the pollution caused by the dye solution. The structure is simple, the cost is low, and it is beneficial to popularize and use in a large area.

An electron microscope ultra-thin section dyeing device provided by the utility model comprises:

The net-carrying device, the net-carrying device includes a support plate, and at least one mesh groove for placing the metal mesh of the ultra-thin section is uniformly arranged on one end surface of the support plate;

The containing device, the containing device comprises a transparent dyeing bag for placing the net-carrying device, the opening of the transparent dyeing bag is provided with a sealing strip for sealing;

Water injection assembly, the water injection assembly includes at least one water injection pipe connected through the transparent dyeing bag, the free end of the water injection pipe is connected with a water injection device, and a useful The first valve for controlling opening and closing;

Liquid injection assembly, the liquid injection assembly includes a first liquid injection tube and a second liquid injection tube connected through the transparent dyeing bag respectively, and the first liquid injection tube is connected with dye solution for injecting uranyl acetate The first liquid injection device, and a second valve for controlling opening and closing is provided between the first liquid injection pipe and the first liquid injection device; the second liquid injection pipe is connected with a valve for injecting lead citrate A second liquid injection device for the dye solution, and a third valve for controlling opening and closing is provided between the second liquid injection pipe and the second liquid injection device;

Exhaust device, described exhaust device comprises the exhaust pipe that is connected with described transparent dyeing bag, and the top of described exhaust pipe is higher than the top end of described transparent dyeing bag, and the free end of described exhaust pipe is connected with exhaust filter;

A waste liquid output device, the waste liquid output device includes a drain pipe connected through the transparent dyeing bag, the free end of the drain pipe is connected with a liquid storage bag for storing waste liquid, and the drain pipe is connected to the transparent dyeing bag. A fourth valve for controlling opening and closing is arranged between the liquid storage bags.

Furthermore, it also includes a hook arranged on the top of the transparent dyeing bag.

Furthermore, the net-carrying device further includes a detachable shielding cover arranged on the support plate, and the shielding cover is arranged on the side of the support plate where the mesh groove is arranged.

Furthermore, the shielding cover is slidably connected with the support plate.

Furthermore, the transparent dyeing bag is made of plastic or rubber.

Furthermore, the second liquid injection device is provided with scale indications for reference.

Furthermore, the top of the exhaust pipe is 5-10 cm higher than the top of the transparent dyeing bag.

Furthermore, the exhaust filter device includes a filter chamber, the end of the filter chamber away from the exhaust pipe is provided with at least one exhaust hole, the filter chamber is provided with a filter film layer for filtering dust, And the filter membrane layer is arranged between the exhaust hole and the exhaust pipe.

Furthermore, the first liquid injection device is a syringe.

The utility model also provides an ultra-thin section dyeing machine, which comprises the above-mentioned electron microscope ultra-thin section dyeing device.

The electron microscope ultra-thin section dyeing device and the ultra-thin section dyeing machine provided by the utility model realize the placement of the metal net equipped with the ultra-thin section through the net-carrying device, put the net-carrying device into the holding device for dyeing, and pass water injection The component effectively discharges the air in the device, and at the same time, it also plays a flushing role during the working process of the liquid injection component. The liquid injection component effectively realizes the double dyeing work, and finally the liquid in the whole process is discharged and stored through the waste liquid discharge device. Effectively reduce pollution to the environment and operators. Therefore, the device dyes in a closed environment, which not only improves the dyeing effect, but also effectively reduces pollution. The device has a simple structure and low cost, which is conducive to large-scale promotion and use.

Description of drawings

In order to more clearly illustrate the specific implementation of the utility model or the technical solutions in the prior art, the accompanying drawings that need to be used in the description of the specific implementation or the prior art will be briefly introduced below. Obviously, the following descriptions The accompanying drawings are some implementations of the utility model, and those skilled in the art can also obtain other drawings according to these drawings without any creative work.

Fig. 1 is the schematic structural view of the electron microscope ultra-thin section dyeing device provided by the embodiment of the present invention;

Fig. 2 is a schematic structural diagram of the cooperation between the receiving device and the hook provided by the embodiment of the present invention;

Fig. 3 is the schematic structural view of the net-carrying device provided by the embodiment of the present invention (with a shielding cover);

Fig. 4 is a schematic structural diagram of the net-carrying device provided by the embodiment of the present invention (without a shielding cover).

Icons: 1 - net carrying device; 2 - support plate; 3 - mesh tank; 4 - transparent dyeing bag; 5 - sealing strip; 6 - water injection pipe; 7 - water injection device; 8 - first valve; 9 - first injection Liquid pipe; 10-second liquid injection pipe; 11-first liquid injection device; 12-second valve; 13-second liquid injection device; 14-third valve; 15-exhaust pipe; 16-exhaust filter Device; 17-drainage pipe; 18-fluid storage bag; 19-the fourth valve; 20-hook; 21-shielding cover.

detailed description

The technical solutions of the utility model will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are part of the embodiments of the utility model, but not all of them. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" The orientation or positional relationship indicated by etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, use a specific The azimuth structure and operation, therefore can not be construed as the limitation of the present utility model. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and should not be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless otherwise clearly stipulated and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a flexible connection. Detachable connection, or integral connection; it can be mechanical connection or electrical connection; it can be direct connection or indirect connection through an intermediary, and it can be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention in specific situations.

The electron microscope ultra-thin section dyeing device and the ultra-thin section dyeing machine provided by the utility model realize the placement of the metal net equipped with the ultra-thin section through the net-carrying device, put the net-carrying device into the holding device for dyeing, and pass water injection The component effectively discharges the air in the device, and at the same time, it also plays a flushing role during the working process of the liquid injection component. The liquid injection component effectively realizes the double dyeing work, and finally the liquid in the whole process is discharged and stored through the waste liquid discharge device. Effectively reduce pollution to the environment and operators. Therefore, the device dyes in a closed environment, which not only improves the dyeing effect, but also effectively reduces pollution. The device has a simple structure and low cost, which is conducive to large-scale promotion and use.

According to Fig. 1 to Fig. 4, the utility model provides an electron microscope ultra-thin section staining device, which includes a net carrying device 1, a holding device, a water injection component, a liquid injection component, an exhaust device and a waste liquid output device.

Referring to FIG. 1 , according to FIG. 3 to FIG. 4 , the net-carrying device 1 includes a support plate 2 , and at least one mesh groove 3 for placing a metal net is evenly distributed on one end surface of the support plate.

In the embodiment of the present utility model, several net grooves are set as required, and a metal mesh is placed in each mesh groove, and the metal mesh is used to place ultra-thin slices, so that several ultra-thin slices can be dyed on a support plate, It can realize batch dyeing and improve work efficiency. Of course, it can also dye an ultra-thin section. It can be set according to needs, as long as it meets the needs of use.

In the embodiment of the utility model, the metal mesh adopts copper mesh, and the diameter of the copper mesh is 3mm. Such a setting meets the placement requirements of stained ultrathin sections. Of course, other metals and size settings can also be used, as long as the usage requirements are met.

According to Fig. 1 to Fig. 2, the containing device includes a transparent dyeing bag 4 for placing the net-carrying device, and a sealing strip 5 for sealing is provided at the opening of the transparent dyeing bag.

In the embodiment of the utility model, the transparent dyeing bag can be set to just accommodate the size of the net-carrying device, which not only makes full use of the space, but also does not waste the dyeing solution. In addition, the transparent dyeing bag is set in a transparent structure, which can be easily observed For internal dyeing and dyeing, when the net-carrying device is put into the transparent dyeing bag, the sealing effect of the transparent dyeing bag is realized by sealing the sealing strip, so as to reduce air pollution and damage to the operator. When it is necessary to take or put the net When installing, you only need to open the sealing strip, which is convenient and simple to operate.

As shown in Figure 1, the water injection assembly includes at least one water injection pipe 6 connected through the transparent dyeing bag, the free end of the water injection pipe is connected with a water injection device 7, and a switch for controlling the opening and closing First valve 8.

In the embodiment of the utility model, the water injection operation into the transparent dyeing bag is realized through the water injection pipe and the water injection device. When water injection is required, the first valve can be opened, and when the water injection is completed, the first valve is closed to achieve the airtight effect , The setting of the water injection component can discharge the air in the transparent dyeing bag through water injection, and can also realize the flushing effect on the dyeing process.

In the embodiment of the utility model, the water injection device is detachably arranged on the water injection pipe, so that the water injection device can be replaced as required, and storage is relatively easy. In addition, the water injection component is arranged at the upper position of the transparent dyeing bag, which is convenient for water injection and flushing.

In the embodiment of the utility model, the water injection pipe is a hose made of rubber or plastic material, and of course other materials can also be used.

According to Fig. 1, the liquid injection assembly comprises the first liquid injection pipe 9 and the second liquid injection pipe 10 connected through the transparent dyeing bag respectively, and the first liquid injection pipe is connected with the first liquid injection pipe for injecting uranyl acetate dye solution. A liquid injection device 11, and a second valve 12 for controlling opening and closing is arranged between the first liquid injection pipe and the first liquid injection device; the second liquid injection pipe is connected with a second injection pipe for injecting lead citrate dye solution Liquid device 13, and a third valve 14 for controlling opening and closing is provided between the second liquid injection pipe and the second liquid injection device.

In the embodiment of the present utility model, the first liquid injection pipe is connected through the transparent dyeing bag, and the injection of the uranyl acetate dye solution is realized through the first liquid injection pipe and the first liquid injection device. The opening and closing respectively realize the effect of liquid injection and airtightness.

In the embodiment of the present utility model, the second liquid injection pipe is connected through the transparent dyeing bag, and the injection of the lead citrate dye solution is realized through the second liquid injection pipe and the second liquid injection device. The liquid injection and sealing effect can be achieved respectively.

Two different dyeing solutions are operated successively to achieve double dyeing effect.

In the embodiment of the present utility model, the first liquid injection pipe and the second liquid injection pipe are hoses made of rubber or plastic, and of course other materials can also be used.

In the embodiment of the present utility model, the first liquid injection device is detachably arranged on the first liquid injection pipe, and similarly, the second liquid injection device is also detachably arranged on the second liquid injection pipe In this way, the first liquid injection device and the second liquid injection device can be replaced as required, and storage is relatively easy. In addition, the liquid injection component is set at the upper position of the transparent dyeing bag, which is convenient for liquid injection and dyeing.

According to shown in Figure 1, exhaust device comprises the exhaust pipe 15 that is connected through with transparent dyeing bag, and the top of exhaust pipe is higher than the top end of transparent dyeing bag, and the free end of exhaust pipe is connected with exhaust filter device 16.

In this utility model embodiment, the exhaust pipe is connected through the transparent dyeing bag, and the free end of the exhaust pipe is connected with an exhaust filter device, which can effectively prevent the external environment from polluting the dyeing process in the transparent dyeing bag, and at the same time It can meet the exhaust effect on transparent dyeing bags.

In the embodiment of the utility model, when the device is in working state, the top of the exhaust pipe is higher than the top of the transparent dyeing bag, so that the pressure requirement during liquid injection and discharge can be met.

In the embodiment of the present utility model, the exhaust pipe is arranged at the lower position of the transparent dyeing bag, which is convenient for exhausting air. At the same time, the exhaust pipe is made of plastic or rubber to meet the needs of use, and the cost is low and easy to replace. Other materials can be used as long as they meet the requirements of use.

As shown in Figure 1, the waste liquid output device includes a liquid discharge pipe 17 connected through the transparent dyeing bag, and the free end of the liquid discharge pipe is connected with a liquid storage bag 18 for storing waste liquid, between the liquid discharge pipe and the liquid storage bag A fourth valve 19 for controlling opening and closing is provided.

In the embodiment of the utility model, the liquid discharge pipe is connected through the transparent dyeing bag, so that the liquid discharge effect can be realized, and the discharged liquid is stored in the liquid storage bag connected with the liquid discharge pipe to avoid environmental pollution. In addition, through the first Four valves realize the opening and closing between the liquid discharge pipe and the liquid storage bag, so as to realize the effects of liquid discharge and airtightness respectively.

In the embodiment of the utility model, the drain pipe is arranged at the bottom of the transparent dyeing bag to facilitate liquid draining. Meanwhile, the drain pipe is made of plastic or rubber.

According to Fig. 1 to Fig. 2, the device also includes a hook 20 arranged on the top of the transparent dyeing bag.

In the embodiment of the utility model, when in use, it can be hung on the corresponding bracket through the hook, so as to facilitate the operation of dyeing.

As shown in FIG. 3 , the net-carrying device further includes a detachable shielding cover 21 arranged on the support plate, and the shielding cover is arranged on the side of the support plate where the mesh groove is arranged.

In the embodiment of the utility model, a cover is provided on the support plate to prevent the metal mesh from detaching from the support plate, and at the same time, it can protect the ultra-thin slices and prevent pollution; when dyeing, the dye solution enters the In the gap between the support plate and the shielding cover, and in contact with the metal mesh, the shielding cover adopts a transparent structure, which can directly observe the dyeing situation of the ultra-thin section.

The shielding cover is slidably connected with the support plate.

In the embodiment of the utility model, the side wall of the support plate is provided with a corresponding chute, and the shielding cover cooperates with the chute through the provided slide bar to realize the relative sliding connection with the support plate. It is simple and convenient to realize the disassembly and assembly of the support plate. Of course, other structures can also be used to realize sliding connection, or other detachable methods can be used, as long as the use requirements are met.

The transparent dyeing bag is made of plastic or rubber.

In the embodiment of the utility model, the transparent dyeing bag is made of plastic or rubber to meet the requirements of use, and the cost is low and easy to replace. Of course, other materials can also be used, as long as the materials meet the requirements of use.

The second liquid injection device is provided with scale indications for reference.

In the embodiment of the present invention, the injection of the dye solution is performed through the second liquid injection device, and the second liquid injection device is provided with a scale indicator, so that the injection situation can be easily known. Similarly, scale indications can be set on both the first liquid injection device and the water injection device.

The height of the top of the exhaust pipe higher than the top of the transparent dyeing bag is 5-10 cm.

In the embodiment of the utility model, the height of the top of the exhaust pipe from the top of the transparent dyeing bag is 5-10 cm, and such a height setting can meet the pressure requirement.

The exhaust filter device includes a filter chamber, the end of the filter chamber away from the exhaust pipe is provided with at least one vent hole, the filter chamber is provided with a filter film layer for filtering dust, and the filter film layer is arranged between the vent hole and the exhaust port. between the tubes.

In the embodiment of the utility model, the exhaust hole meets the use of the exhaust device in the exhaust process, and the filter film layer arranged between the exhaust hole and the exhaust pipe can effectively achieve the filtering effect and avoid the external environment from affecting the transparent dyeing bag. pollution within.

The first liquid injection device is a syringe.

In the embodiment of the utility model, the first liquid injection device is a syringe, which is simple in structure, low in cost, and easy to replace and purchase. The same second liquid injection device and water injection device can also use syringes. The syringe can be 20ml or 50ml, of course, other measurements can also be used.

The usage examples of this program are as follows:

Step 1, placing the net-carrying device equipped with metal mesh inside the transparent dyeing bag;

Step 2, ensuring that the first valve, the second valve, the third valve and the fourth valve are in a closed state;

Step 3, sealing the sealing strip on the transparent dyeing bag;

Step 4. Hang the transparent dyeing bag on the corresponding bracket, and fix the exhaust pipe on the transparent dyeing bag on the bracket, and the height of the top of the exhaust pipe is 5-10cm higher than the top of the transparent dyeing bag;

Step 5. Open the first valve, keep the other valves closed, inject distilled water through the water injection device, and observe the state of the liquid level in the exhaust pipe. When the liquid level is at the same height as the top of the transparent dyeing bag, the water injection device stops injecting, the first valve closed;

Step 6. Open the second valve, keep the other valves closed, slowly inject about 3-5ml of uranyl acetate dye solution into the transparent dyeing bag with the first liquid injection device, close the second valve, and let it stand until the required dyeing time Time, in this step, the metal mesh is placed in the uranyl acetate dye solution for 15 to 45 minutes;

Step 7. Open the fourth valve, keep the other valves closed, drain the liquid in the transparent dyeing bag into the liquid storage bag through the discharge pipe, and open the first valve when there is no liquid in the exhaust pipe or the liquid level no longer drops , inject distilled water with a water injection device, and repeat this operation 1 to 2 times; wherein, the amount of injected distilled water is 3 to 5 ml, which is equivalent to the amount of uranyl acetate dye solution injected in step 6;

Step 8, close the fourth valve, inject distilled water with the water injection device, when the liquid level is consistent with the height of the top of the transparent dyeing bag, stop the water injection, and close the first valve;

Step 9, open the third valve, keep the other valves closed, inject about 3-5ml of lead citrate dye solution with the second liquid injection device, close the third valve, and let it stand for the required dyeing time. In this step, Put the metal mesh in the lead citrate dye solution for 10 to 35 minutes;

Step 10. Open the fourth valve, keep the other valves closed, drain the liquid in the transparent dyeing bag into the liquid storage bag through the drain pipe, and open the first valve when there is no liquid in the exhaust pipe or the liquid level no longer drops , inject distilled water with water injection device, repeat this operation 2～3 times; Wherein, the amount of injecting distilled water is 3～5ml, promptly gets final product with the amount of the lead citrate dye solution that injects in step nine;

Step 11. Close all valves, open the sealing strip, take out the net carrying device from the inside of the transparent dyeing bag, and the dyeing is completed. The waste liquid in the liquid storage bag can be handed over to the environmental protection department for pollution-free unified treatment.

The electron microscope ultra-thin section dyeing device provided by the utility model realizes the placement of the metal net equipped with the ultra-thin section through the net-carrying device, puts the net-carrying device into the containing device for dyeing, and effectively removes the metal mesh in the device through the water injection component. The air is discharged, and at the same time, it also plays a flushing role during the working process of the liquid injection component. The liquid injection component effectively realizes double dyeing work, and finally the liquid in the whole process is discharged and stored through the waste liquid discharge device, which effectively reduces the impact on the environment and operation. Therefore, this device dyes in a closed environment, which not only improves the dyeing effect, but also effectively reduces pollution, and the device has a simple structure and low cost, which is conducive to large-scale promotion and use.

The utility model adopts the electron microscope ultra-thin section dyeing device, the user no longer directly contacts the dye solution, and the waste liquid will not cause environmental pollution through centralized collection. Pollution, environmental protection and practical.

The utility model also provides an ultra-thin section dyeing machine, which comprises the above-mentioned electron microscope ultra-thin section dyeing device.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present utility model, and are not intended to limit it; although the present utility model has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand : It can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements to some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions depart from the embodiments of the present utility model Scope of technical solutions.

Claims (10)

1. An electron microscope ultrathin section staining device, is characterized in that, comprises: The net-carrying device, the net-carrying device includes a support plate, and at least one mesh groove for placing the metal mesh of the ultra-thin section is uniformly arranged on one end surface of the support plate; The containing device, the containing device comprises a transparent dyeing bag for placing the net-carrying device, the opening of the transparent dyeing bag is provided with a sealing strip for sealing; Water injection assembly, the water injection assembly includes at least one water injection pipe connected through the transparent dyeing bag, the free end of the water injection pipe is connected with a water injection device, and a useful The first valve for controlling opening and closing; Liquid injection assembly, the liquid injection assembly includes a first liquid injection tube and a second liquid injection tube connected through the transparent dyeing bag respectively, and the first liquid injection tube is connected with dye solution for injecting uranyl acetate The first liquid injection device, and a second valve for controlling opening and closing is provided between the first liquid injection pipe and the first liquid injection device; the second liquid injection pipe is connected with a valve for injecting lead citrate A second liquid injection device for the dye solution, and a third valve for controlling opening and closing is provided between the second liquid injection pipe and the second liquid injection device; Exhaust device, described exhaust device comprises the exhaust pipe that is connected with described transparent dyeing bag, and the top of described exhaust pipe is higher than the top end of described transparent dyeing bag, and the free end of described exhaust pipe is connected with exhaust filter; A waste liquid output device, the waste liquid output device includes a drain pipe connected through the transparent dyeing bag, the free end of the drain pipe is connected with a liquid storage bag for storing waste liquid, and the drain pipe is connected to the transparent dyeing bag. A fourth valve for controlling opening and closing is arranged between the liquid storage bags. 2. The electron microscope ultrathin section staining device according to claim 1, further comprising a hook arranged on the top of the transparent staining bag. 3. The electron microscope ultra-thin section dyeing device according to claim 1, characterized in that, the grid-carrying device also includes a detachable shielding cover arranged on the support plate, and the shielding cover is arranged on the The support plate is set on one side of the net groove. 4 . The electron microscope ultrathin section staining device according to claim 3 , wherein the shielding cover is slidably connected with the support plate. 5 . 5. The electron microscope ultrathin section staining device according to claim 1, wherein the transparent staining bag is made of plastic or rubber. 6. The electron microscope ultra-thin section staining device according to claim 1, characterized in that, the second liquid injection device is provided with a scale indication for reference. 7 . The electron microscope ultra-thin section dyeing device according to claim 1 , wherein the height of the top of the exhaust pipe from the top of the transparent dyeing bag is 5-10 cm. 8. The electron microscope ultra-thin section staining device according to claim 1, wherein the exhaust filter device comprises a filter chamber, and at least one exhaust pipe is provided at the end of the filter chamber away from the exhaust pipe. The filter chamber is provided with a filter membrane layer for filtering dust, and the filter membrane layer is arranged between the exhaust hole and the exhaust pipe. 9. The electron microscope ultra-thin section staining device according to claim 1, wherein the first liquid injection device is a syringe. 10. An ultrathin section staining machine, characterized by comprising the electron microscope ultrathin section staining device according to any one of claims 1-9.