CN206486544U - A kind of tissue cell culture device - Google Patents

Abstract

The utility model discloses a tissue cell culture device, which comprises a constant temperature box and a cell-taking device. The cell-taking device is composed of a transparent plastic tube, a rubber block, a housing, a straight rod and two pistons, and the transparent plastic tube is fixed and installed horizontally. On the outer wall on the left side of the incubator, one end of the transparent plastic tube is open and the other end is sealed. The rubber block is set inside the transparent plastic tube. There is a groove on the surface of the rubber block, and the housing is placed in the groove. Two The pistons are respectively fixedly installed on both sides of the rubber block, the transparent plastic tube is sealed by the piston, the outer wall of the upper end of the transparent plastic tube is provided with a first through hole, the straight rod is fixedly installed on the left piston, and one end of the straight rod is fixed Install a handle. The utility model has a simple structure, and there is no need to open the sealed door of the incubator when extracting cells, which avoids affecting the temperature and environment in the incubator, and at the same time prevents bacteria or other substances from entering the incubator, increasing the reliability and safety of the experiment sex.

Description

A tissue cell culture device

technical field

The utility model relates to a tissue cell culture device.

Background technique

Tissue culture is a technique for aseptically taking out tissue blocks and cell groups from individuals of multicellular organisms, and making them continue to live under appropriate conditions. Cultivating cells to study the effects of drugs is also being used to make vaccines. There are two ways of tissue culture, one is cell culture, and the other is tissue block culture.

It is one of the more commonly used methods for tissue culture to maintain larger tissue blocks and organ blocks in a three-dimensional structure; when culturing, choose various shapes, For materials of various sizes, the culture medium is mainly used as a culture medium.

During tissue culture, it is necessary to maintain stable environmental conditions, but now when detecting the activity of cell tissue or extracting tissue cells, it is necessary to open the door of the incubator, and then close the door after completion. The continuous opening and closing of the sealed door of the incubator greatly affects It affects the constant temperature environment and brings the risk of pollution at the same time.

Utility model content

In order to solve the above problems, the utility model provides a simple structure, easy to use, no need to open the sealed door of the incubator when extracting cells, avoiding the influence on the temperature and environment in the incubator, and also avoiding the entry of bacteria or other substances. Tissue cell culture device in the incubator,

In order to achieve the above object, the technical scheme that the utility model takes is:

A tissue cell culture device, including a constant temperature box and a cell removal device, the cell removal device is composed of a transparent plastic tube, a rubber block, a placement shell with an upper end opening, a straight rod and two pistons, and the transparent plastic tube is fixed horizontally on a thermostat. On the outer wall on the left side of the box, one end of the transparent plastic tube is set inside the incubator, and the other end passes through the outer wall to extend to the outside world. One end of the transparent plastic tube is open outside, and the end inside the incubator is sealed. The rubber block is set on the transparent plastic tube. Inside, the cross-section of the rubber block is set in a hemispherical structure. There is a groove on the surface of the rubber block that matches the placement shell. The placement shell is placed in the groove, and the two pistons are respectively fixed on the two sides of the rubber block. On the side, the outer ring surfaces of the two pistons are all against the inner ring surface of the transparent plastic tube, and the transparent plastic tube is sealed by the piston, and a first through hole is arranged on the outer wall of the upper end of the transparent plastic tube, and the first through hole is connected to the outer wall of the transparent plastic tube. The shells are relatively arranged, and the straight rod is fixedly installed on the piston on the left side. One end of the straight rod extends to the outside along the opening on the transparent plastic tube, and a handle is fixedly installed.

As a preferred technical solution, a supporting plate is fixedly installed on the bottom surface inside the incubator. The supporting plate is provided with a plurality of fixing holes for fixing the culture dish. A petri dish is provided, and a second thread is provided on the outer surface of the petri dish, and the second thread on the petri dish engages with the first screw in the fixing hole.

As a preferred technical solution, a toughened glass is fixedly installed on the upper end of the thermostat, and a second through hole is arranged on the toughened glass, and a cover plate is arranged in the second through hole, and the cover plate is threadedly connected with the second through hole, and the second through hole A rubber glove is fixedly installed at the lower end of the hole, the opening of the rubber glove communicates with the second through hole, and the second through hole is also sealed by the rubber glove.

As a preferred technical solution, a dropper placement frame is fixedly installed on the inner wall of one side of the incubator, and more than one dropper is provided on the dropper placement frame.

The utility model has the following beneficial effects: the utility model is simple in structure, easy to use, and does not need to open the sealed door of the incubator when extracting cells, which avoids affecting the temperature and environment in the incubator, and at the same time prevents bacteria or other substances from entering the constant temperature Inside the box, the reliability and safety of the experiment are increased.

Description of drawings

Fig. 1 is the overall structure schematic diagram of the utility model embodiment;

Fig. 2 is a partial sectional view at A-A in Fig. 1 .

detailed description

In order to make the purpose and advantages of the utility model clearer, the utility model will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model, and are not intended to limit the utility model.

As shown in Fig. 1 and Fig. 2, the utility model embodiment provides a kind of tissue cell culture device, comprises incubator 1 and take cell device, and described cell take device is made up of transparent plastic tube 9, rubber block 12, upper open The shell 11, the straight rod 14 and two pistons 13 are placed, and the transparent plastic tube 9 is fixed horizontally on the outer wall on the left side of the incubator 1. One end of the transparent plastic tube 9 is arranged inside the incubator 1, and the other end extends through the outer wall to Outside, one end of the transparent plastic tube 9 is open outside, and one end inside the thermostat 1 is sealed. The rubber block 12 is arranged inside the transparent plastic tube 9. The cross section of the rubber block 12 is arranged in a hemispherical structure. There is a groove matching the housing 11, the housing 11 is placed in the groove, and the two pistons 13 are fixedly installed on both sides of the rubber block 12 respectively. The inner ring surface of the pipe 9 is offset, and the transparent plastic pipe 9 is sealed by the piston 13. The outer wall of the upper end of the transparent plastic pipe 9 is provided with a first through hole 10, and the first through hole 10 is arranged opposite to the housing 11. The straight rod 14 is fixedly installed on the piston 13 on the left side, and one end of the straight rod 14 extends to the outside world along the opening on the transparent plastic tube 9, and a handle 15 is fixedly installed.

In this embodiment, a supporting plate 2 is fixedly installed on the bottom surface of the incubator 1 inside, and the supporting plate 2 is provided with a plurality of fixing holes for fixing the culture dish, and a first thread is provided on the inner ring surface of the fixing holes, and the fixing holes A petri dish 3 is arranged inside, and a second thread is provided on the outer surface of the petri dish 3, and the second thread on the petri dish 3 is engaged with the first screw in the fixing hole.

In this embodiment, a toughened glass 16 is fixedly installed on the upper end of the thermostat 1, and the toughened glass 16 is provided with a second through hole 7, and a cover plate 8 is arranged in the second through hole 7, and the gap between the cover plate 8 and the second through hole 7 A rubber glove 6 is fixedly installed at the lower end of the second through hole 7, the opening of the rubber glove 6 communicates with the second through hole 7, and the second through hole is also sealed by the rubber glove 6.

In this embodiment, a dropper placement frame 4 is fixedly installed on the inner wall of the incubator 1 , and more than one dropper 5 is arranged on the dropper placement frame 4 .

When it is necessary to extract tissue cells, open the cover plate on the second through hole, and put your hand into the rubber glove, wherein, through the sealing of the rubber glove, external bacteria or other substances are prevented from entering the constant temperature through the second through hole. In the box, it also prevents the heat in the incubator from radiating to the outside. Among them, the situation inside the incubator can be effectively seen through the tempered glass. When the hand is inserted into the incubator through the rubber glove, pick up the dropper, and Draw a part of the cell liquid (cells and culture fluid) in the culture dish through the dropper. After completion, move the dropper to the first through hole of the transparent plastic tube, and insert the open end of the dropper along the first through hole. Put it in the case, squeeze out the cell fluid in the dropper, so that the cell liquid enters the placement case, at this time, pull the piston outward through the handle, the movement of the piston drives the movement of the rubber block and the case placed in the rubber block, making the left side The piston moves to the outside along the opening of the transparent plastic tube, and at the same time, the housing moves to the outside. Take the housing out of the rubber block to facilitate its detection. After taking it out, place a new housing. It is convenient for an experiment, wherein the piston on the right cannot be moved out of the transparent plastic tube, and the piston on the right needs to ensure the sealing of the transparent plastic tube.

This specific implementation is simple in structure and easy to use. When extracting cells, it is not necessary to open the sealed door of the incubator, which avoids affecting the temperature and environment changes in the incubator, and also prevents bacteria or other substances from entering the incubator. reliability and safety.

The above is only a preferred embodiment of the utility model, it should be pointed out that for those of ordinary skill in the art, without departing from the principle of the utility model, some improvements and modifications can also be made. These improvements and modifications It should also be regarded as the protection scope of the present utility model.

Claims (4)

1. A tissue cell culture device, characterized in that: it comprises an incubator and a cell-getting device, the cell-getting device is made up of a transparent plastic tube, a rubber block, a placement shell with an upper end opening, a straight rod and two pistons, and the transparent plastic The tube is fixed horizontally on the outer wall on the left side of the incubator. One end of the transparent plastic tube is set inside the incubator, and the other end passes through the outer wall to extend to the outside. The block is set inside the transparent plastic tube. The cross-section of the rubber block is set in a hemispherical structure. There is a groove on the surface of the rubber block that matches the placement shell. The placement shell is placed in the groove, and the two pistons are fixed respectively. Installed on both sides of the rubber block, the outer ring surfaces of the two pistons are all against the inner ring surface of the transparent plastic tube, the transparent plastic tube is sealed by the piston, and the outer wall of the upper end of the transparent plastic tube is provided with a first through hole, The first through hole is arranged opposite to the placement shell, the straight rod is fixedly mounted on the left piston, and one end of the straight rod extends to the outside along the opening on the transparent plastic tube, and a handle is fixedly installed. 2. The tissue cell culture device according to claim 1, characterized in that: a supporting plate is fixedly installed on the bottom surface of the incubator inside, the supporting plate is provided with a plurality of fixing holes for fixing culture dishes, and the inner ring surface of the fixing holes A first thread is provided on each of the tops, a petri dish is provided in each fixing hole, and a second thread is provided on the outer surface of the petri dish, and the second thread on the petri dish engages and connects with the first screw in the fixing hole. 3. The tissue cell culture device according to claim 1, characterized in that: a toughened glass is fixedly installed on the upper end of the incubator, the toughened glass is provided with a second through hole, and a cover plate is arranged in the second through hole, and the cover plate and The second through holes are threadedly connected, and a rubber glove is fixedly installed at the lower end of the second through holes. The opening of the rubber glove communicates with the second through holes, and the second through holes are also sealed by the rubber glove. 4. The tissue cell culture device according to claim 1, characterized in that: a dropper holder is fixedly installed on the inner wall of one side of the incubator, and more than one dropper is provided on the dropper holder.