CN206173341U - Inoculation device of seeding tank - Google Patents
Inoculation device of seeding tank Download PDFInfo
- Publication number
- CN206173341U CN206173341U CN201621227296.1U CN201621227296U CN206173341U CN 206173341 U CN206173341 U CN 206173341U CN 201621227296 U CN201621227296 U CN 201621227296U CN 206173341 U CN206173341 U CN 206173341U
- Authority
- CN
- China
- Prior art keywords
- inoculation
- seed tank
- peristaltic pump
- bottle
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000011081 inoculation Methods 0.000 title claims abstract description 112
- 238000010899 nucleation Methods 0.000 title abstract 3
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 28
- 239000010409 thin film Substances 0.000 claims description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 239000000741 silica gel Substances 0.000 claims description 12
- 229910002027 silica gel Inorganic materials 0.000 claims description 12
- 238000003780 insertion Methods 0.000 claims description 4
- 230000037431 insertion Effects 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 9
- 239000007788 liquid Substances 0.000 abstract description 8
- 238000000855 fermentation Methods 0.000 abstract description 2
- 230000004151 fermentation Effects 0.000 abstract description 2
- WLNBMPZUVDTASE-HXIISURNSA-N (2r,3r,4s,5r)-2-amino-3,4,5,6-tetrahydroxyhexanal;sulfuric acid Chemical compound [O-]S([O-])(=O)=O.O=C[C@H]([NH3+])[C@@H](O)[C@H](O)[C@H](O)CO.O=C[C@H]([NH3+])[C@@H](O)[C@H](O)[C@H](O)CO WLNBMPZUVDTASE-HXIISURNSA-N 0.000 abstract 3
- 241000233866 Fungi Species 0.000 abstract 1
- 206010037660 Pyrexia Diseases 0.000 abstract 1
- 230000003833 cell viability Effects 0.000 abstract 1
- 238000001125 extrusion Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 229920000742 Cotton Polymers 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 125000001246 bromo group Chemical group Br* 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 208000031481 Pathologic Constriction Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a biological fermentation device field, aim at provides an inoculation device of seeding tank, inoculation device of seeding tank, including dona tub, inoculation bottle, inoculation pipe and peristaltic pump, be equipped with inoculation opening on the dona tub, be equipped with the film that seals the inoculation opening passageway in the inoculation opening, the exit end of inoculation pipe is connected with the inoculating needle, the inoculating needle passes the film and inserts in the dona tub, the entrance point of inoculation pipe stretches into in the inoculation bottle, be equipped with the peristaltic pump on the inoculation pipe. The utility model has the advantages of be equipped with the film that seals the inoculation opening passageway in inoculation opening, adopt the inoculation of flame inoculation mode, through flame high -temperature sterilization inoculation opening space in order to reduce cell viability in the space, effectively avoid miscellaneous fungus to pollute, pair of rollers inoculation pipe hocket extrusion through the peristaltic pump comes pump sending inoculation liquid with the process of release, puies forward top grafting kind efficiency.
Description
Technical field
This utility model is related to biological fermentation device field, more particularly to a kind of seed tank inoculating device.
Background technology
In fermenting and producing, microorganism seed need in advance be cultivated in gnotobasiss be put in a sterile chamber first,
When seed tank input culture medium sterilization is cooled to technological requirement temperature, seed is accessed in seed tank, the process is inoculation.Mesh
It is front generally using pressure differential method by seed liquor suck seed tank in, i.e., in advance on seed tank reserve inoculation mouth, by inoculation mouth and kind
Sub- tank sterilization together, there is installation inoculation mouth at inoculation mouth, and first carries out sky with bromo geramine around inoculation mouth in inoculation
Between sterilize, clean inoculation mouth with 75% ethanol afterwards, finally remove inoculation bottle silica gel pipe end gauze directly and inoculation mouth docking；
By seed canister incoming gas and the adjustment of aerofluxuss, pressure inside the tank is repeatedly lifted up and reduces, so as to seed liquor is sucked into seed tank.Using
This vaccination ways, it is not thorough to space disinfection to there is bromo geramine, during inoculation inoculation mouth do not clean it is clean, while workman is in behaviour
The problems such as easily making a fault on work.
Utility model content
The purpose of this utility model is to overcome drawbacks described above, there is provided one kind can be prevented effectively from living contaminantses, sealing
Good, simple to operate seed tank inoculating device.
In order to solve above-mentioned technical problem, the technical solution adopted in the utility model is：
A kind of seed tank inoculating device, including seed tank, inoculation bottle, inoculated tube and peristaltic pump, the seed tank is provided with
Inoculation mouth, is provided with the thin film of closing inoculation mouth passage in the inoculation mouth, the port of export of the inoculated tube is connected with Inoculating needle, institute
Inoculating needle is stated through in thin film insertion seed tank, the entrance point of the inoculated tube is stretched in inoculation bottle, and the inoculated tube is provided with
Peristaltic pump.
Further, the inoculation mouth include base, the first pipe fitting and lid, the tube chamber welds with base on seed tank, institute
The annular boss that base is provided with disposed thread is stated, the bottom of first pipe fitting is threadedly coupled with annular boss, described first
The top of pipe fitting is threadedly coupled with lid.
Further, the lid is provided with seal washer.
Further, the thin film is silica gel piece.
Further, the inoculated tube is silica gel tube.
Further, the entrance point of the inoculated tube stretches into inoculation bottle bottom.
Further, the inoculation bottle has workbench with peristaltic pump, and the workbench is higher than seed tank.
The beneficial effects of the utility model are：The thin film of closing inoculation mouth passage is provided with inoculation mouth, using flame
Vaccination ways are inoculated with, by flame high-temperature sterilization inoculation mouth space to reduce space in Viable detection, be prevented effectively from miscellaneous bacteria dirty
Dye；Increase peristaltic pump, the process for alternately being extruded by the pair of rollers inoculated tube of peristaltic pump and being discharged is carried pumping inoculation liquid
High inoculation efficiency, also reduces the operation frequency of seed tank lifting pressure itself.
Description of the drawings
Fig. 1 is the structure chart of this utility model embodiment seed tank inoculating device.
Fig. 2 is the explosive view of the inoculation mouth of this utility model embodiment seed tank inoculating device.
Label declaration：
1- seed tanks；2- inoculation bottles；3- inoculation mouths；4- peristaltic pumps；5- inoculated tubes；6- workbench；31- bases；32- ring-types
Boss；The pipe fittings of 33- first；34- lids；35- thin film.
Specific embodiment
To describe technology contents of the present utility model, structural features in detail, purpose and effect being realized, below in conjunction with enforcement
Mode simultaneously coordinates accompanying drawing to be explained in detail.
The design of this utility model most critical is：An inoculation mouth is directly welded on seed tank face, and in inoculation mouth
The thin film of closing inoculation mouth passage is set, is inoculated with using flame inoculation mode, while being handed over by the pair of rollers inoculated tube of peristaltic pump
Inoculation liquid is pumped for the process for being extruded and being discharged.
Refer to shown in Fig. 1-2, the seed tank inoculating device of the present embodiment, including seed tank 1, inoculation bottle 2, inoculated tube 5
With peristaltic pump 4, the seed tank 1 is provided with inoculation mouth 3, the thin film 35 of the closing passage of inoculation mouth 3 is provided with the inoculation mouth 3,
The port of export of the inoculated tube 5 is connected with Inoculating needle, and the Inoculating needle is inserted in seed tank 1 through thin film 35, the inoculated tube 5
Entrance point stretch in inoculation bottle 2, the inoculated tube 5 is provided with peristaltic pump 4.
The course of work of the present utility model is：The inoculation mouth 3 of a sealing, inoculation of outwarding winding directly are welded on seed tank 1
Mouthfuls 3 lid, puts an ethanol Cotton Gossypii on thin film 35, and screws the lid of inoculation mouth 3, and to seed tank 1 sterilizing is carried out
Shi Buyong outwards arranges steam；Hold inoculation liquid inoculation bottle 2 be sent to after, first the tank pressure flow of seed tank 1 is transferred to position, during inoculation
Without adjusting again；Inoculation mouth of first outwarding winding 3, and the ethanol Cotton Gossypii on thin film 35 is lighted, then steeped with one around inoculation mouth 3
Space around the flame rod high-temperature sterilization inoculation mouth 3 of ethanol Cotton Gossypii；Connect an inoculation syringe needle in the port of export of inoculated tube 5,
The port of export of inoculated tube 5 is bandaged with gauze, the entrance point of inoculated tube 5 is stretched into into the inner bottom part of seed bottle 2, and by seed bottle 2
Opening bandage, inoculation bottle 2 and inoculated tube 5 carry out ultraviolet lamp sterilization sterilization in Culture Collection；After having sterilized, inoculation bottle 2
The entrance point of the inoculated tube 5 of connection passes through peristaltic pump, and the roller being fixed on peristaltic pump 4；During inoculation, as long as removing inoculated tube
Gauze on 5 port of export end, inoculation syringe needle is placed on flame and is baked 30 seconds or so, with inoculation punctures inoculation mouth 3
Thin film 35 so that in Inoculating needle insertion seed tank 1, the whole flame of inoculation all can not extinguish；Peristaltic pump 4 is opened, inoculation liquid
It is extracted in seed tank 1；Inoculation screws inoculation mouth 3 after terminating.
Knowable to foregoing description, the beneficial effects of the utility model are：Closing inoculation mouth passage is provided with inoculation mouth
Thin film, be inoculated with using flame inoculation mode, by flame high-temperature sterilization inoculation mouth space to reduce space in Viable detection,
It is prevented effectively from living contaminantses；Increase peristaltic pump, the process for alternately extruding and discharging by the pair of rollers inoculated tube of peristaltic pump
To pump inoculation liquid, inoculation efficiency is improved, also reduce the operation frequency of seed tank lifting pressure itself.
Further, the inoculation mouth includes base 31, the first pipe fitting 33 and lid 34, and the base 31 is welded on seed
On tank 1, the base 31 is provided with the annular boss 32 of disposed thread, bottom and the spiral shell of annular boss 32 of first pipe fitting 33
Stricture of vagina connects, and the top of first pipe fitting 33 is threadedly coupled with lid 34.
Further, the lid 34 is provided with seal washer.
Seen from the above description, it is threaded connection and sealing gasket sealing so that sealing effectiveness more preferably, is difficult microbiological contamination.
Further, the thin film 35 is silica gel piece.
Seen from the above description, the good airproof performance of silica gel piece, while can be replaced as needed.
Further, the inoculated tube 5 is silica gel tube.
Seen from the above description, because silica gel tube has, splendid resilience and permanent deformation are little, and meeting repeatedly to sterilize
Requirement, therefore with silica gel tube as inoculated tube, meet the requirement of sterilizing, while can use cooperatively with the roller of peristaltic pump,
It is easy to extrude and discharges.
Further, the entrance point of the inoculated tube 5 stretches into the bottom of inoculation bottle 2.
Seen from the above description, the entrance point of inoculated tube stretches into inoculation bottle bottom, and inoculation liquid can be made to transfer completely into seed
In tank, also make seeded process meet sealing requirements, reduce the impact of viable bacteria in air.
Further, the inoculation bottle 2 has workbench 6 with peristaltic pump 4, and the workbench 6 is higher than seed tank 1.
Seen from the above description, workbench plays a part of to support inoculation bottle and peristaltic pump, more compact structure；With seed tank
Certain difference in height is formed, seeded process is more easy to carry out.
Refer to shown in Fig. 1-2, embodiment of the present utility model is：
A kind of seed tank inoculating device, including seed tank 1, inoculation bottle 2, inoculated tube 5 and peristaltic pump 4, on the seed tank 1
Inoculation mouth 3 is provided with, the inoculation mouth includes base 31, the first pipe fitting 33 and lid 34, and the base 31 is welded on seed tank 1
On, the base 31 is provided with the annular boss 32 of disposed thread, bottom and the screw thread of annular boss 32 of first pipe fitting 33
Connection, the top of first pipe fitting 33 is threadedly coupled with lid 34；The lid 34 is provided with seal washer；The inoculation mouth
The thin film 35 of the closing passage of inoculation mouth 3 is provided with 3, the port of export of the inoculated tube 5 is connected with Inoculating needle, and the Inoculating needle is passed through
In the insertion seed tank 1 of thin film 35, the entrance point of the inoculated tube 5 is stretched in inoculation bottle 2, and the inoculated tube 5 is provided with peristaltic pump
4, the thin film 35 is silica gel piece, and the inoculated tube 5 is silica gel tube；The entrance point of the inoculated tube 5 stretches into the bottom of inoculation bottle 2；
The inoculation bottle 2 has workbench 6 with peristaltic pump 4, and the workbench 6 is higher than seed tank 1.
In sum, the seed tank inoculating device that this utility model is provided, one sealing structure of welding connects on seed tank
Mouth is planted, inoculation mouth is sealed by double thread connection and sealing pad so that sealing effectiveness more preferably, is difficult microbiological contamination；It is provided with inoculation mouth
The thin film of closing inoculation mouth passage, is inoculated with using flame inoculation mode, is inoculated with Inoculating needle, is connect by flame high-temperature sterilization
Viable detection during mouth space is planted to reduce space, is prevented effectively from living contaminantses；Increase peristaltic pump, by the pair of rollers of peristaltic pump
Inoculated tube is alternately extruded and the process that discharges is pumping inoculation liquid, improves inoculation efficiency, is also reduced seed tank itself and is lifted
The operation frequency of pressure.
Embodiment of the present utility model is the foregoing is only, the scope of the claims of the present utility model is not thereby limited, it is every
The equivalent structure made using this utility model description and accompanying drawing content or equivalent flow conversion, or be directly or indirectly used in
Other related technical fields, are included in the same manner in scope of patent protection of the present utility model.
Claims (7)
1. a kind of seed tank inoculating device, it is characterised in that：Including seed tank, inoculation bottle, inoculated tube and peristaltic pump, the seed
Tank is provided with inoculation mouth, and the thin film of closing inoculation mouth passage is provided with the inoculation mouth, and the port of export of the inoculated tube is connected with
Inoculating needle, through in thin film insertion seed tank, the entrance point of the inoculated tube is stretched in inoculation bottle the Inoculating needle, the inoculation
Pipe is provided with peristaltic pump.
2. seed tank inoculating device according to claim 1, it is characterised in that：The inoculation mouth includes base, first pipe
Part and lid, on seed tank, the base is provided with the annular boss of disposed thread, first pipe fitting to the tube chamber welds with base
Bottom be threadedly coupled with annular boss, the top of first pipe fitting is threadedly coupled with lid.
3. seed tank inoculating device according to claim 2, it is characterised in that：The lid is provided with seal washer.
4. seed tank inoculating device according to claim 1, it is characterised in that：The thin film is silica gel piece.
5. seed tank inoculating device according to claim 1, it is characterised in that：The inoculated tube is silica gel tube.
6. seed tank inoculating device according to claim 1, it is characterised in that：The entrance point of the inoculated tube stretches into inoculation
Bottom of bottle portion.
7. seed tank inoculating device according to claim 1, it is characterised in that：The inoculation bottle has work with peristaltic pump
Make platform, the workbench is higher than seed tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621227296.1U CN206173341U (en) | 2016-11-15 | 2016-11-15 | Inoculation device of seeding tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621227296.1U CN206173341U (en) | 2016-11-15 | 2016-11-15 | Inoculation device of seeding tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206173341U true CN206173341U (en) | 2017-05-17 |
Family
ID=58685189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621227296.1U Active CN206173341U (en) | 2016-11-15 | 2016-11-15 | Inoculation device of seeding tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206173341U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110862919A (en) * | 2019-12-16 | 2020-03-06 | 江苏澳创生物科技有限公司 | Fermentation strain inoculation device and inoculation method |
-
2016
- 2016-11-15 CN CN201621227296.1U patent/CN206173341U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110862919A (en) * | 2019-12-16 | 2020-03-06 | 江苏澳创生物科技有限公司 | Fermentation strain inoculation device and inoculation method |
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| GR01 | Patent grant |