CN205966413U - PL level ultramicron liquid supplementation device - Google Patents

Abstract

The utility model provides a pL-level ultra-micro-volume replenishment device. The rehydration device includes five parts: a pipetting needle, a motion controller, a glass microtube connector, a flow control device, and a liquid delivery device; among them, the motion controller controls the up and down movement of the pipetting needle; the glass microtube connector is based on the principle of the connector and Designed based on the principle of capillary phenomenon; the flow control device can adjust the speed and amount of fluid infusion to meet the needs of liquids with different viscosities; the liquid delivery device connects the flow control device with the glass microtube connector through the connection interface, which is a glass microtube connector Provide a small amount of liquid. The method of the utility model sucks the liquid in the connector into the microtube through the micro pressure difference inside and outside the microtube generated during the up and down movement of the pipetting needle in the capillary microtube, so as to realize the replenishment of pL level liquid in the microtube. The utility model is suitable for pL-level ultra-micro-quantity continuous liquid replenishment of liquids with various viscosities, and the flow rate is easy to observe and adjust.

Description

pL-level ultra-micro-volume rehydration device

technical field

The utility model relates to the technical field of spotting, in particular to a liquid replenishing device for a pL (picoliter) level ultra-micro-volume spotting platform for micro-assembly.

Background technique

Spotting technology has a wide range of applications, from semiconductor packaging industry, integrated circuit industry, SMT/PCB assembly industry to general industrial welding, injection coating and sealing spotting, spotting technology plays a vital role. The utility model proposes a new micro-volume continuous liquid replenishment device on the basis of the ultra-micro-volume spotting method proposed by Zhang Qin of South China University of Technology, which solves the problem that the original spotting method cannot continuously replenish liquid. The ultra-micro-volume spotting method is to use the micro-droplets adsorbed by the tip of the pipette needle to realize ultra-micro-volume spotting when the pipette needle passes through the liquid in the glass microtube. Among them, the storage of the spotting liquid is located in the glass microtube, and its principle is to fill the glass microtube with liquid by means of capillary phenomenon. In the original device, the glass microtube device that relies on capillary phenomenon to realize the liquid storage glass microtube is limited by the storage principle and its own structure. The capillary cannot store a large amount of liquid, and cannot realize real-time micro-volume continuous replenishment of the capillary. Therefore, the continuous operation of sample application cannot be realized in the original ultra-micro-volume sample application device, and the sample application operation needs to be stopped to replenish the liquid. Limited by this, the original method cannot meet the requirements for continuous operation of the ultra-micro-volume spotting platform, and is only suitable for a very small amount of discontinuous spotting applications. The method proposed by the utility model can realize the micro-volume continuous liquid replenishment of the ultra-micro-volume spotting device; on the basis of retaining the original glass microtube device which relies on capillary phenomenon to realize liquid storage, and relies on the principle of the connecting device, a utility model is developed for a real-time liquid replenishment device . It solves the problem that the original ultra-micro-volume spotting platform cannot work continuously.

Utility model content

In order to overcome the deficiencies of the above-mentioned prior art, the utility model retains the original glass microtube device that relies on capillary phenomenon to realize liquid storage, and relies on the principle of the connector to design a new pL-level ultra-micro-volume liquid replenishment device and method.

The purpose of the utility model is at least achieved by one of the following technical solutions.

A pL-level ultra-micro-volume rehydration device, including a pipette needle, a motion controller, a glass microtube connector, a flow control device, and a liquid delivery device; the glass microtube connector is designed based on the principle of a connector and the principle of capillarity It is composed of a connector, a capillary microtube, and a liquid overflow port; the axial centerline of the pipetting needle coincides with the axial centerline of the capillary microtube part in the glass microtube connector;

The motion controller is fixedly connected with the pipetting needle to control the up and down movement of the pipetting needle in the glass microtube connector;

The flow control device is composed of a communication interface, an infusion hose, a flow rate regulator, a drip pot and a needle mouth connected in sequence, the communication interface is connected with the glass microtube connector, and is connected with the liquid delivery device through the needle mouth;

The liquid delivery device is connected with a glass microtube connector through a flow control device to provide liquid.

Further, in the glass microtube communicator, the bottom end of one end of the communicator is connected to the capillary microtube, and the upper half of the capillary microtube extends into the inner cavity of the communicator, and the lower part extends out of the communicator; the liquid overflow port It is arranged on the bypass side of the glass microtube connector, and the liquid overflow port is located outside the bypass branch pipe of the connector.

Further, the upper part of the capillary microtube extends into the inner cavity of the communicator, and the protruding length is 10mm-15mm; the lower part protrudes out of the communicator, and the protruding length is 5mm-10mm; the inner diameter of the capillary microtube ranges from 0.8- 1mm; the inner diameter of the main liquid storage cavity in the connector ranges from 25mm to 26mm, and the inner diameter of the bypass liquid feeding tube of the connector ranges from 3 to 4mm.

Further, in the glass microtube communicator, for liquids with different viscosity properties, different liquid overflow heights are used to control the distance between the liquid level line and the upper end of the capillary microtube, and the liquid level height is higher than that of the capillary microtube. 1-2mm.

Further, the pipetting needle is made of ultra-fine and precise electrical discharge machining of a tungsten wire needle, and the size of the needle tip is determined according to the requirement of liquid dispensing volume.

Further, the motion controller is composed of a linear servo motor, a servo driver, and a pipetting needle holder; the pipetting needle holder adopts a three-jaw chuck structure to clamp the pipetting needle.

Further, the communicator includes a main liquid storage chamber, a communication pipe, and a bypass rehydration pipe. The tube and the main liquid storage chamber of the liquid; the capillary microtube is used to store the liquid in contact with the pipetting needle in the ultra-micro-volume spotting device; the liquid overflow port is used to control the height of the liquid surface line of the main liquid storage chamber.

Furthermore, the flow control device can adjust the speed and amount of liquid replenishment to meet the needs of liquids with different viscosities. The flow control device is composed of a communication interface, an infusion hose, a flow rate regulator, a drip pot, and a needle nozzle. Among them, the communication interface is used to connect the flow control device with the glass microtube connector to bypass the infusion tube, the infusion hose is used to transport the liquid, the flow rate regulator is used to adjust the infusion speed, and the drip pot is used to observe the flow rate of the infusion liquid and remove the infusion hose. The air bubble, the needle nozzle is used to communicate with the flow control device and the liquid delivery device.

Applicable to the liquid replenishment method of the pL-level ultra-micro-volume liquid replenishment device, including: the micro-volume replenishment is to clamp the pipette needle by the pipette needle clamp in the motion controller, and the linear servo motor drives the up and down movement process of the pipette needle in the microtube of the connector The small pressure difference inside and outside the microtube generated in the microtube sucks the liquid in the connector into the microtube to realize the replenishment of the pL level liquid in the microtube.

Further, the control of the volume of fluid replacement is to control the liquid volume of a small amount of fluid supplementation when the pipette needle moves up and down by changing the geometric size of the tip of the pipette needle and the frequency of the up and down movement, so as to meet the requirements for different volumes of fluid supplementation under the volume dimension of pL, where The frequency of up and down movement is determined by the pulse frequency received by the motion controller.

Further, by adjusting the rotation of the pulley in the flow rate adjustment device, the diameter of the infusion hose in the flow control device is controlled to adjust the infusion flow rate, so as to realize the function of replenishing liquids with different viscosities; Observation of the dripping frequency of the medium droplet to obtain real-time rehydration flow rate.

Compared with the prior art, the beneficial effects of the utility model are:

(1) Realized the real-time rehydration function of the pL-level ultra-micro-volume spotting device.

(2) Realized the continuous and uninterrupted spotting function of the pL-level ultra-micro-volume spotting device.

(3) Realized the monitoring function of the liquid replenishment rate of the pL-level ultra-micro-volume spotting device.

(4) Realized the function of adjusting the liquid replenishment speed of the pL-level ultra-micro-volume spotting device.

(5) Realized the replenishment operation of the pL-level ultra-micro-volume spotting device for different liquid viscosities.

(6) Realized the function of uniform liquid discharge by the pressure type liquid replenishment method.

Description of drawings

Fig. 1 is a structural schematic diagram of a pL-level micro-volume continuous liquid replenishment device of an example of the utility model.

Fig. 2 is a schematic structural view of a glass microtube connector of an example of the present invention.

Fig. 3a-Fig. 3c are schematic diagrams of pL-level micro-infusion fluid in the example of the utility model.

Fig. 4a is the schematic diagram of the flow control device of the utility model example;

Fig. 4b is a schematic cross-sectional view of the nozzle.

Fig. 5a-Fig. 5d are flow monitoring schematic diagrams of the examples of the present utility model.

Fig. 6 is a schematic diagram of the flow regulating device of the example of the utility model.

In the figure: 1-glass microtube connector; 101-communicator; 102-capillary microtube; 103-liquid overflow port; 2-pipetting needle motion controller; 3-pitting needle; 4-first support; 5-liquid; 6-flow control device; 601-communication interface; 602-infusion hose; 603-flow rate regulator; 60301-pulley linear guide; 60302-pulley; Delivery device; 701-liquid storage tank; 702-pressure device; 8-second support; 9-micro droplet.

detailed description

The purpose of the utility model will be further described in detail below in conjunction with the accompanying drawings and specific examples. The examples cannot be repeated here one by one, but the implementation of the utility model is not therefore limited to the following examples. Unless otherwise specified, the materials and processing methods used in the present invention are conventional materials and processing methods in the technical field.

As shown in Figure 1, a pL-level ultra-micro-volume replenishment device suitable for an ultra-micro-volume spotting platform includes a pipetting needle 3, a motion controller 2, a glass microtube connector 1, a flow control device 6, and a liquid delivery device 7 five parts.

The glass microtube communicator 1 includes a first bracket 4 , a communicator 101 installed on the first bracket 4 , a capillary microtube 102 installed at the bottom of one end of the communicator, and a liquid overflow port 103 .

As shown in Fig. 2 and Fig. 3, the micro-infusion is to use the motion controller 2 to drive the pipetting needle 3 to move up and down in the capillary micro-tube 102 to generate a micro-pressure difference between the inside and outside of the micro-tube, and to suck the liquid in the connector 101 In the microtube, the supplement of pL level liquid in the microtube is realized. The liquid 5 in the connector 101 is replenished into the capillary microtube 102 in the form of tiny droplets 9 to realize the continuous trace replenishment of the liquid 5 in the capillary microtube 102, and then complete the ultra-micro-volume spotting operation. In different glass microtube connectors 1 , the protruding size of the capillary microtube 102 in the connector 101 and the height of the liquid overflow port can be adjusted to meet the requirements of liquids with different viscosities.

As shown in Figure 4a, the flow control device 6 is composed of a communication interface 601, an infusion hose 602, a flow rate regulator 603, a drip pot 604 and a needle nozzle 605 connected in sequence, and the communication interface 601 and the connector 101 are bypassed. The pipe interface is connected. Both ends of the communication interface are respectively connected to the infusion hose 602 and the bypass pipe interface of the connector 101, the flow rate regulator 603 is set on the outer end of the infusion hose 602, and the two ends of the infusion hose 602 are connected to the communication interface 601 respectively. It is connected with the lower port of the drip pot 604, and the drip pot is connected with the infusion hose 602 and the needle mouth 605 respectively. Through the flow control device 6, the distance between the liquid level in the connector and the upper end port of the capillary microtube 102 is maintained at 1-2 mm, so as to prevent the capillary microtube 102 from being unable to rely on the liquid in the adsorption tube to cause liquid leakage due to the excessive amount of liquid 5 in the connector. The problem of leakage.

As shown in Figure 5, the flow rate regulator 603 in the flow control device 6 adjusts the diameter of the infusion hose 602 by adjusting the upper and lower positions of the pulley 60302 in the pulley linear guide 60301, thereby controlling the liquid flow rate.

As shown in Figure 6, the dripping pot 604 in the flow control device 6 obtains the fluid replenishment speed by observing the falling frequency of the droplets, and then adjusts the flow controller to maintain the liquid surface line in the connector and the upper port of the capillary microtube 102. The distance is maintained at 1-2mm.

The liquid delivery device 7 is fixed by the second bracket 8, and the liquid delivery device 7 is connected to the glass microtube connector 1 through the connection port 601 at the end of the flow control device 6, and the liquid delivery device 7 is delivered to the liquid through the pressure device 702. The flow control device 6 supplies liquid 5 to the glass microtube connector 1 through the flow control device 6 .

The connector 101 includes a main liquid storage chamber, a communication pipe, and a bypass liquid replenishment pipe, wherein the main liquid liquid storage chamber is used to store the liquid delivered by the liquid replenishment device, and supplies liquid to the capillary microtube 102, and the communication pipe is used to communicate with the bypass liquid replenishment pipe and the main liquid storage chamber; the capillary microtube 102 is used to store the liquid in contact with the pipetting needle 3 in the ultra-micro-volume spotting device; the liquid overflow port 103 is used to control the height of the liquid level in the main liquid storage chamber.

The flow control device 6 can adjust the speed and amount of liquid replenishment to meet the needs of liquids with different viscosities. The flow control device 6 is composed of a communication interface 601 , an infusion hose 602 , a flow rate regulator 603 , a drip pot 604 and a needle nozzle 605 . Among them, the communication interface 601 is used to connect the flow control device 6 and the glass microtube connector 1 to bypass the liquid infusion tube, the infusion hose 602 is used to transport the liquid 5, the flow rate regulator 603 is used to adjust the liquid infusion speed, and the drip pot 604 is used to observe the infusion liquid The liquid flow rate and the removal of air bubbles in the infusion hose, the needle nozzle 605 is used to connect the flow control device 6 and the liquid delivery device 7 .

In this example, the micro-infusion is to clamp the pipette needle 3 by the pipette needle clamp in the motion controller 2, and the linear servo motor drives the pipette needle 3 to move up and down in the microtube of the connector. The micro-pressure difference sucks the liquid in the connector into the microtube to realize the pL-level liquid replenishment in the microtube.

The control of the amount of liquid replenishment is to control the amount of liquid replenishment in a small amount of liquid replenishment when the pipette needle 3 moves up and down by changing the geometric size of the tip of the pipetting needle 3 and the frequency of the up and down movement, so as to meet the requirements for different liquid replenishment volumes in the pL level volume dimension, where the up and down The motion frequency is determined by the pulse frequency received by the motion controller 2.

By adjusting the rotation of the pulley 60302 in the flow rate regulating device 603, the diameter of the infusion hose 602 in the flow control device 6 is controlled to adjust the infusion flow rate, so as to realize the function of replenishing liquids with different viscosities; The observation of the dripping frequency of the liquid droplets in the drip pot 604 obtains the real-time fluid replacement flow rate.

Claims (6)

1. A pL-level ultra-micro-volume rehydration device, characterized by comprising a pipetting needle (3), a motion controller (2), a glass microtube connector (1), a flow control device (6) and a liquid delivery device (7); The glass microtube connector (1) is designed according to the principle of the connector and the principle of capillarity, and is composed of the connector (101), the capillary microtube (102), and the liquid overflow port (103); The axial centerline of the liquid needle (3) coincides with the axial centerline of the capillary microtube (102) in the glass microtube connector (1); The motion controller (2) is fixedly connected with the pipetting needle (3) to control the up and down movement of the pipetting needle (3) in the glass microtube connector (1); The flow control device (6) is composed of a communication interface (601), an infusion hose (602), a flow rate regulator (603), a drip pot (604) and a needle nozzle (605) connected in sequence, and the communication interface (601) Communicate with the glass microtube connector (1), and connect with the liquid delivery device (7) through the needle nozzle (605); The liquid delivery device (7) is connected to the glass microtube connector (1) through the flow control device (6) to provide the liquid (5). 2. The pL-level ultra-micro-volume rehydration device according to claim 1, characterized in that: in the glass microtube connector (1), the bottom end of one end of the connector (101) is connected to a capillary microtube (102), And the upper part of the capillary microtube (102) extends into the inner cavity of the communicator (101), and the lower part extends out of the communicator (101); the liquid overflow port (103) is arranged in the bypass of the glass microtube communicator (1) On one side, the liquid overflow port (103) is located outside the bypass branch pipe of the connector (101). 3. The pL-level ultra-micro-volume rehydration device according to claim 2, characterized in that: the upper half of the capillary microtube (102) extends into the inner cavity of the connector (101), and the length of the extension is 10mm-15mm; the lower half Partially protruding from the connector (101), the protruding length is 5mm-10mm; the inner diameter of the capillary tube (102) ranges from 0.8-1mm; the inner diameter of the main liquid storage chamber in the connector (101) ranges from 25mm-26mm, the connector (101) The inner diameter of the bypass fluid feeding tube ranges from 3-4mm. 4. The pL-level ultra-micro-volume rehydration device according to claim 2, characterized in that: in the glass microtube connector (1), different liquid overflow ports (103) are used for liquids with different viscosity properties Height, control the distance between the liquid surface line of the liquid and the upper end of the capillary microtube (102), the liquid level is 1-2mm higher than the capillary microtube (102). 5. The pL-level ultra-micro-volume rehydration device according to claim 2, characterized in that: the pipetting needle (3) is made of ultra-fine precision electric discharge machining of a tungsten wire needle, and the size of the needle tip is determined according to the requirement of liquid distribution. 6. The pL-level ultra-micro-volume rehydration device according to claim 1, characterized in that: the motion controller (2) is composed of a linear servo motor, a servo driver, and a pipetting needle holder; the pipetting needle holder adopts three claws The chuck structure is used to clamp the pipetting needle (3).