CN205962202U - Examine wooden tissue culture device - Google Patents

Examine wooden tissue culture device Download PDF

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Publication number
CN205962202U
CN205962202U CN201620956502.6U CN201620956502U CN205962202U CN 205962202 U CN205962202 U CN 205962202U CN 201620956502 U CN201620956502 U CN 201620956502U CN 205962202 U CN205962202 U CN 205962202U
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CN
China
Prior art keywords
wooden
tissue culture
examine
culture device
rotating base
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Expired - Fee Related
Application number
CN201620956502.6U
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Chinese (zh)
Inventor
刘中华
张志录
张巧莲
张炎
蔡清
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Pingdingshan University
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Pingdingshan University
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Priority to CN201620956502.6U priority Critical patent/CN205962202U/en
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Publication of CN205962202U publication Critical patent/CN205962202U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses an examine wooden tissue culture device, pegging graft including transparent cylindricality shell, tray, base, rotating base, sealed and covering the body and upper cover, rotating base locates on the base to the tray is equipped with atomizing device on locating rotating base in the orientation of tray on the upper cover, atomizing device places in to the tray examines wood group to bank up the seedling with earth to carry out the humidification. The utility model discloses an examine wooden tissue culture device, the tissue culture device that utilizes this application is cultivateed the stem section of examining wood, tames before step D's transplantation from the drawing materials of step A, step B's differentiation and hyperplasia, step C's rooting culture, all goes on in this tissue culture device, need not to change culture tank, the poultry feeders management of being convenient for. The device that uses this utility model is cultivated examines wood, and the time of breaking up, breed, takeing root and tame is short, and the root beard and hair nearly, the culture dish of unified management, use is few, and survival rate of seedlings reaches the percent and more than 92%, has better market perspective.

Description

Examine wooden tissue culture device
Technical field
This utility model is related to technical field, more particularly to examines wooden tissue culture device.
Background technology
Examine come wooden for evergreen dwarf shrub(Height and about 1 meter of hat width), spring in autumn and winter at florescence to next year, pattern have white, Red as fire color, pink etc., resist cold, resistance to coastal environment and light frost.Resistance to full sun is it is desirable that there are some to shade.Almost in any soil bar Under part can well-grown, avoid hydrops, preferably gently sandy and well-drained soil.Can be with loose frangible complete corruption during plantation Ripe mulching material, the soil around root area gently breaks up favourable plant growing.Plant is in the fall with early spring fertilization effect more Good, the fertilizer of available comprehensive nutrition but the content of phosphorus is low, if the slow released fertilizer applying without phosphorus formula is more preferable.Periodically water, special It is not in the season of growth, deeply water and be conducive to growing up healthy and sound and blooming of plant.Periodically prune, Chang Dingduan prunes increases branch's number Amount can promote bushes formation, also by increase the next adolescence spend number.Professional etiquette is entered by pierres such as cuttage, tissue cultures Modelling is bred.
But existing examine wooden in cuttage and numerous soon during, carry out tissue culture using traditional tissue culture tank, its reproductive efficiency Low, because the reasons such as temperature, humidity cause during tissue culture, yellow leaf, long speckle or stem section wilting phenomenon, survival rate is only 80% about.And examine wooden more rare at home, and need to introduce from Australia or Spain, relatively costly.In order to solve Above-mentioned technical problem, this utility model provides a kind of quickly tissue culture and examines wooden device, and examines using this device is numerous soon The method of wood.
Content of the invention
In order to overcome the above-mentioned deficiencies of the prior art, this utility model provides and examines wooden tissue culture device, its purpose Be to improve and examine wooden tissue culture's speed and efficiency, be finally reached may cultivate efficiently quick, at low cost examine wooden Seedling, transplanting are examined wooden so that examining the wooden mood that can popularize at home, please for people's offer in uninteresting winter.
This utility model be employed technical scheme comprise that:Examine wooden tissue culture device, be placed in aseptic interior use, including Transparent cylindrical shell, pallet, base, rotating base, sealed grafting cover body and Shang Gai, rotating base on base, and , on rotating base, sealed grafting cover body is located at the above side of covering, and extends to transparent cylindrical shell upper position for pallet; On cover and be provided with atomising device towards the direction of pallet, atomising device to place in pallet examine wooden tissue cultured seedling carry out plus Wet, atomising device connects source of sterile water.Of the present utility model examine come wooden tissue culture device, using rotating base drive be placed in it On pallet rotation, thus drive be placed in pallet examine come wooden tissue cultured seedling rotated so that cultivation stage examine come wooden Seedling fully can merge with its corresponding culture medium in differentiation, reproductive stage and root cultivation stage of taking root, and accelerates the above-mentioned stage The differentiation examining wooden seedling, the efficiency bred, take root root and domestication.Additionally, of the present utility model examine on wooden culture apparatuses It is additionally provided with atomising device, in real time moisturizing is carried out to the wooden seedling of examining of culture.
Further, tissue culture device on cover and be additionally provided with temperature control equipment and temperature indicating device, transparent post The inside of shape shell is additionally provided with temperature sensor, and temperature sensor connects temperature control equipment and temperature indicating device, and it is right to be easy to Temperature in tissue culture device is understood, convenient cultivation human users, and efficiency is higher.
Further, above cover and be also connected with blowing device, blowing device connects temperature control equipment, temperature control equipment sets There is excess temperature alarm device, according to the device internal temperature of display on temperature control equipment, blow cooling in real time, single dress Put control temperature it is achieved that being controlled to the internal medium of single tissue culture device it is ensured that its local temperature is too high causes Wooden seedling loss, improve its survival rate, reduce cost.
Further, atomising device connects blowing device, and blowing device is worked with atomising device simultaneously, has preferably cold But effect.
Compared with prior art, the beneficial effects of the utility model are:Of the present utility model examine come wooden tissue culture device, Pallet rotation placed on it is driven using rotating base, thus driving the wooden tissue cultured seedling of examining being placed in pallet to be rotated, Wooden seedling of examining in cultivation stage was filled with its corresponding culture medium in differentiation, reproductive stage and root culture stage Divide and merge, that accelerates the above-mentioned stage examines the differentiation of wooden seedling, the efficiency bred, take root and tame.Additionally, it is of the present utility model Examine and be additionally provided with atomising device on wooden culture apparatuses, in real time moisturizing is carried out to the wooden seedling of examining of culture.
Of the present utility model examine come wooden quick-breeding method, make full use of tissue culture device of the present utility model to examine come wooden Stem section is cultivated, and tames and dociles to the transplanting of step D from the root culture of the drawing materials of step A, the differentiation of step B and propagation, step C Change, all carry out in this tissue culture device, culture tank need not be changed, be easy to poultry feeders management, additionally, this device is permissible Carry out the monitoring of real-time temperature, humidity and illumination, real time record can also be carried out to data, be easy to analyze and examine wooden training Support.Using this utility model apparatus and method cultivate examine come wooden, the time broken up, breed, take root and tame is short, root hair Flourishing, unified management, the culture dish using are few, and transplanting survival rate reaches more than percent 92%, before having the application of preferable market Scape.
Brief description
Fig. 1 is the structural representation of an embodiment examining wooden tissue culture device;
Wherein:1- transparent cylindrical shell, 2- pallet, 3- base, 4- rotating base, 5- sealed grafting cover body, 6- covers, 7- atomising device, 8- temperature control equipment, 9- temperature indicating device, 10- temperature sensor, 11- blowing device, 12- overtemperature report Alarm device, 13- source of sterile water.
Specific embodiment
In order to deepen to understanding of the present utility model, with reference to the accompanying drawings and examples to this utility model furtherly Bright, this embodiment is only used for explaining this utility model, protection domain of the present utility model is not constituted and limits.
As shown in figure 1, examine come wooden tissue culture device, including transparent cylindrical shell 1, pallet 2, base 3, rotating base 4, Sealed grafting cover body 5 and upper lid 6, rotating base 4 is on base 3, and pallet 2 is on rotating base 4, sealed slotting Connect cover body 5 above upper lid 6, and extend to transparent cylindrical shell 1 upper position;On upper lid 6 towards on the direction of pallet 2 It is provided with atomising device 7, atomising device 7 humidifies to the wooden tissue cultured seedling of examining placed in pallet 2, and atomising device 7 connects aseptic Water source 13.Of the present utility model examine wooden tissue culture device, drive pallet 2 placed on it to rotate using rotating base, from And drive the wooden tissue cultured seedling of examining being placed in pallet to be rotated so that the wooden seedling of examining in cultivation stage is being broken up, bred rank Section and the root culture stage fully can merge with its corresponding culture medium, accelerate the above-mentioned stage examine wooden seedling differentiation, Breeding, the efficiency taken root and tame.Additionally, of the present utility model examining is additionally provided with atomising device 7, in real time on next wooden culture apparatuses Moisturizing is carried out to the wooden seedling of examining of culture.
In the above-described embodiments, the upper lid 6 of tissue culture device is additionally provided with temperature control equipment 8 and temperature indicating device 9, the inside of transparent cylindrical shell 1 is additionally provided with temperature sensor 10, and temperature sensor 10 connects temperature control equipment 8 and temperature shows Showing device 9, is easy to the temperature in tissue culture device is understood, convenient cultivation human users, and efficiency is higher.
In the above-described embodiments, upper lid 6 is also connected with blowing device 11, blowing device 11 connects temperature control equipment 8, temperature Degree control device 8 is provided with excess temperature alarm device 12, according to the device internal temperature of display on temperature control equipment, carries out in real time Quenching, single assembly controls temperature it is achieved that be controlled to the internal medium of single tissue culture device, it is to avoid because The too high nursery stock loss causing of local temperature, improves survival rate, reduces cost.Atomising device 7 connects blowing device 11, blows Wind apparatus 11 are worked with atomising device 7 simultaneously, have more preferable cooling effect.
Carry out examining wooden method numerous soon using above-mentioned culture apparatuses of knitting, comprise the steps:
A. draw materials, obtain and examine wooden current-year branch, cut off blade, retain 1 stem section, tap water rinses 12-16min, Take full bud, add that Tween 80 is scrubbed, tap water is rinsed well with saturation detergent water successively, the ethanol with 75%, 0.1% mercuric chloride leaching Bubble 10-12min, aseptic water washing 5-6 time, put in tissue culture device;
B. break up and breed, carried out using the division culture medium that 1/2MS+BA1.0mg/L+NAA0.1mg/L, pH value are 5.7 Differentiation culture, the aseptic seedling obtaining in step A is inoculated on division culture medium, and each tissue culture device inoculates 200-300 Block, the tissue culture device inoculated is put into intensity of illumination 2500Lx, under conditions of temperature 23-28 DEG C, daily illumination 10- 14h, cultivates 28-32 days, period is atomized to aseptic seedling every three days or dries;Additionally, after differentiation culture starts, daily Open the rotating base of tissue culture device, rotating base drives the aseptic seedling in pallet slowly to be rotated, rotary speed Turn daily for 230-290, obtain examining wooden adventitious bud;
The adventitious bud obtaining in step B is cut into reservation bud in gnotobasiss by C. root culture, retains the height of bud For 1.5-2.2cm, make root media, the reservation bud that cutting is obtained is placed in root media and continues culture 30-36 days; When root culture starts, open the rotating base of tissue culture device daily daytime, rotating base drives aseptic in pallet Seedling is slowly rotated, and rotary speed turns daily for 200-260, and wherein root media at least includes IBA1.0mg/L;
D. before transplanting, domestication, the temperature of the temperature control equipment of tissue culture device is set in 20-26 DEG C, intensity of illumination 2500Lx, daily illumination 6-8h, tame 3-5 days;Then open lid, tame 5-8 days in room temperature;
E. transplant and examine wooden seedling:
A. sieve after turfy soil insolation, by turfy soil, Vermiculitum and fine sand by volume 4:1:1 ratio mixing, uses 0.2% potassium permanganate sprays to be mixed all, then covers more than 20h with plastic foil and carries out disinfection, obtains substrate of planting, base of planting Matter loads nutritive cube;
B. the tissue culture tamed Seedling of taking root is taken out from culture bottle, wash culture medium off, plant into equipped with substrate of planting Nutritive cube, accomplish the tip of a root down, stem is upright, shallow embedding, light pressure and floating;
C. the pallet having planted Seedling is moved in booth, greenhouse-environment condition control 18-25 DEG C, relative humidity 70%-90%, Intensity of illumination 2000-10000Lx, start that in 7 days ventilation time is short, ventilation frequency will height, be gradually increased ventilation after 7 days, Tissue culture's Seedling of taking root is tamed 13-15 days in booth.
In the above-described embodiments, when the root hair length that the root culture of step C extremely retains bud is 0.4-0.6cm, especially During 0.5cm, carry out taming before the transplanting of step D, step D is tamed to when examining wooden seedling root hair length to 3-5cm, especially 4cm When, the wooden seedling of examining tamed is placed in continuation domestication in booth, it is ensured that the root hair length examining wooden seedling is 5- after booth domestication Transplanted during 10cm, especially 6-8cm.Step E is transplanted and is examined wooden seedling, and its specific time is 9 and October, the effect of transplanting Rate is higher.
Of the present utility model examine wooden tissue culture device, drive pallet 2 placed on it to rotate using rotating base, from And drive the wooden tissue cultured seedling of examining being placed in pallet to be rotated so that the wooden seedling of examining in cultivation stage is being broken up, bred rank Section and the root culture stage fully can merge with its corresponding culture medium, accelerate the above-mentioned stage examine wooden seedling differentiation, Breeding, the efficiency taken root and tame.Of the present utility model examine come wooden method numerous soon, make full use of tissue of the present utility model Culture apparatuses are cultivated to examining wooden stem section, from the training of taking root of the drawing materials of step A, the differentiation of step B and propagation, step C Support to the transplanting of step D domestication, all carry out in this tissue culture device, culture tank need not be changed, be easy to poultry feeders Management, additionally, this device can carry out the monitoring of real-time temperature, humidity and illumination, can also carry out real time record to data, It is easy to analyze and examine wooden culture.Using this utility model apparatus and method cultivate examine come wooden, break up, breed, take root with And the time of domestication is short, root hair is flourishing, unified management, the culture dish that uses are few, and transplanting survival rate reaches more than percent 92%, There is preferable market application foreground.
What embodiment of the present utility model was announced is preferred embodiment, but is not limited thereto, the common skill of this area Art personnel, easily according to above-described embodiment, understand spirit of the present utility model, and make different amplifications and change, but as long as Without departing from spirit of the present utility model, all in protection domain of the present utility model.

Claims (3)

1. examine wooden tissue culture device it is characterised in that being placed in aseptic interior use, including transparent cylindrical shell(1), pallet (2), base(3), rotating base(4), sealed grafting cover body(5)With upper lid(6), described rotating base(4)Located at base(3) On, and described pallet(2)Located at rotating base(4)On;Described sealed grafting cover body(5)Located at Shang Gai(6)Top, and Extend to transparent cylindrical shell(1)Upper position;Described upper lid(6)On towards pallet(2)Direction be provided with atomising device (7), described atomising device(7)Connect source of sterile water(13), described atomising device(7)To pallet(2)Interior placement examine come wooden group Seedlings cultivating is humidified.
2. according to claim 1 examine wooden tissue culture device it is characterised in that the upper lid of described tissue culture device (6)On be additionally provided with temperature control equipment(8)And temperature indicating device(9), transparent cylindrical shell(1)Inside be additionally provided with temperature pass Sensor(10), described temperature sensor(10)Connect temperature control equipment(8)And temperature indicating device(9).
3. according to claim 2 examine wooden tissue culture device it is characterised in that described upper lid(6)On be also connected with blowing Wind apparatus(11), described blowing device(11)Connect temperature control equipment(8), described temperature control equipment(8)It is provided with overtemperature report Alarm device(12).
CN201620956502.6U 2016-08-29 2016-08-29 Examine wooden tissue culture device Expired - Fee Related CN205962202U (en)

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Application Number Priority Date Filing Date Title
CN201620956502.6U CN205962202U (en) 2016-08-29 2016-08-29 Examine wooden tissue culture device

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Application Number Priority Date Filing Date Title
CN201620956502.6U CN205962202U (en) 2016-08-29 2016-08-29 Examine wooden tissue culture device

Publications (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942039A (en) * 2017-03-23 2017-07-14 陈晴 Middle school student's biology sapling culture apparatus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942039A (en) * 2017-03-23 2017-07-14 陈晴 Middle school student's biology sapling culture apparatus

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170222

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CF01 Termination of patent right due to non-payment of annual fee