CN205596815U - Simple and easy aseptic group banks up device with earth - Google Patents
Simple and easy aseptic group banks up device with earth Download PDFInfo
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- CN205596815U CN205596815U CN201620142478.2U CN201620142478U CN205596815U CN 205596815 U CN205596815 U CN 205596815U CN 201620142478 U CN201620142478 U CN 201620142478U CN 205596815 U CN205596815 U CN 205596815U
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- shower nozzle
- seed
- conveyer belt
- rotating
- length
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- 238000012258 culturing Methods 0.000 claims description 28
- 239000002245 particle Substances 0.000 claims description 21
- 239000007921 spray Substances 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 238000012549 training Methods 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 230000035784 germination Effects 0.000 description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 5
- 235000015278 beef Nutrition 0.000 description 5
- 238000012136 culture method Methods 0.000 description 5
- 229960002163 hydrogen peroxide Drugs 0.000 description 5
- 229960002523 mercuric chloride Drugs 0.000 description 5
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 210000000582 semen Anatomy 0.000 description 5
- 238000002791 soaking Methods 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 244000060924 Brassica campestris Species 0.000 description 2
- 235000005637 Brassica campestris Nutrition 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000972 organotrophic effect Effects 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000009305 arable farming Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Nozzles (AREA)
Abstract
The utility model discloses a simple and easy aseptic group banks up device with earth. The device comprises feed inlet, first shower nozzle, second shower nozzle, third shower nozzle, fourth shower nozzle, the 5th shower nozzle, the 6th shower nozzle, several automatic appearance, rotatory cultivation dish, conveyer belt, and first shower nozzle, second shower nozzle, third shower nozzle, fourth shower nozzle, the 5th shower nozzle, the 6th shower nozzle are located the conveyer belt top. Adjustment conveying belt speed for the seed receive time that first shower nozzle, second shower nozzle, third shower nozzle, fourth shower nozzle, the 5th shower nozzle, the 6th shower nozzle sprayed be 1, 1, 5, 1, 5 respectively, 5min. Several automatic appearance can be separated every seed to the quantity of accurate measurement seed, rotatory cultivation dish is rotatable, makes the even distribution of seed in rotatory cultivation dish. The utility model relates to a rationally, simple manufacture, low cost, convenient operation, the no bacterin of the less plant of seed volume trained to the batch greatly.
Description
Technical field
This utility model belongs to plant nutrient and soil science research field, relates to arable farming bactericidal unit, particularly relates to a kind of simple aseptic group of training device.
Background technology
Along with going deep into and the development of agricultural facility technology of plant nutrient research, aseptic seedling becomes the pith of many research and agricultural production.Especially in organotrophic research, the small-molecule substance such as aminoacid can be decomposed by the microorganisms rapidly utilization, and the most many organotrophic research can only aseptically be carried out.Although soil, water, air can be carried out sterilizing, but, seed adheres to complicated and diversified microorganism, sterilizing complete to seed still lacks device simple to operate, with low cost.This utility model is intended to, by ethanol, hydrogen peroxide, the method for mercuric chloride combination sterilizing, devise a kind of Simple soil bactericidal unit.This device is simple to operate, with low cost, can large batch of cultivation aseptic seedling.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, a kind of simple aseptic group of training device is provided, is made up of charging aperture, the first shower nozzle, the second shower nozzle, the 3rd shower nozzle, the 4th shower nozzle, the 5th shower nozzle, the 6th shower nozzle, automatic particle counter, rotating and culturing dish, conveyer belt;First shower nozzle, the second shower nozzle, the 3rd shower nozzle, the 4th shower nozzle, the 5th shower nozzle, the 6th shower nozzle are positioned at above conveyer belt, charging aperture is positioned at the top of conveyer belt one end, seed is made to fall uniformly on conveyer belt, automatic particle counter is positioned at the bottom of the conveyer belt other end, the communicated band of sterilizing seed is made to fall in automatic particle counter, rotating and culturing dish is positioned at automatic particle counter lower left quarter, and in automatic particle counter, the single seed fallen is evenly distributed in rotating and culturing dish.Shower nozzle length ratio is: the first shower nozzle: the second shower nozzle: the 3rd shower nozzle: the 4th shower nozzle: the 5th shower nozzle: six shower nozzles=1:1:5:1:5:5, first shower nozzle sprays 70% ethanol, second shower nozzle sprays sterilized water, 3rd shower nozzle sprays 10% hydrogenperoxide steam generator, 4th shower nozzle ejection sterilized water, 5th shower nozzle ejection 0.1M mercuric chloride solution, the 6th shower nozzle ejection sterilized water.
Conveyer belt is network structure, flow out from conveyer belt after shower nozzle spraying sterilization of liquids or cleaning seed, in first shower nozzle, in 70% ethanol of ejection, the 3rd shower nozzle, 10% hydrogenperoxide steam generator of ejection, the 5th shower nozzle spray 0.1M mercuric chloride solution reusable edible, adjust line speed so that seed by the time that the first shower nozzle, the second shower nozzle, the 3rd shower nozzle, the 4th shower nozzle, the 5th shower nozzle, the 6th shower nozzle spray be respectively 1,1,5,1,5,5min.
This utility model provides automatic particle counter to be separated by every seed, and accurately measures the quantity of seed, and rotating and culturing dish is rotatable so that seed is distributed in rotating and culturing dish uniformly.This utility model is reasonable in design, makes simple, with low cost, easy to operate, can cultivate the seed volume aseptic seedling compared with plantlet in high volume.
Accompanying drawing explanation
Fig. 1 is this utility model structural representation.
Detailed description of the invention
This utility model is further described in conjunction with the accompanying drawings and embodiments.
Embodiment 1
nullSee Fig. 1,A kind of simple aseptic group of training device,By charging aperture 1、First shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、6th shower nozzle 7、Automatic particle counter 8、Rotating and culturing dish 9、Conveyer belt 10 forms,First shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、6th shower nozzle 7 is positioned at above conveyer belt 10,Charging aperture 1 is positioned at the top of conveyer belt 10 one end,Seed is made to fall uniformly on conveyer belt,Automatic particle counter 8 is positioned at the bottom of the conveyer belt other end,The communicated band of sterilizing seed is fallen in automatic particle counter 8,Rotating and culturing dish 9 is positioned at automatic particle counter 8 lower left quarter,In automatic particle counter 8, the single seed fallen is evenly distributed in rotating and culturing dish 9,First shower nozzle 2 length: the second shower nozzle 3 length: the 3rd shower nozzle 4 length: the 4th shower nozzle 5 length: the 5th shower nozzle 6 length: the 6th shower nozzle 7 length=1:1:5:1:5:5,First shower nozzle 2 sprays 70% ethanol,Second shower nozzle 3 sprays sterilized water,3rd shower nozzle 4 sprays 10% hydrogenperoxide steam generator,4th shower nozzle 5 sprays sterilized water,5th shower nozzle 6 sprays 0.1M mercuric chloride solution,6th shower nozzle 7 sprays sterilized water.
Conveyer belt 10 is network structure, inflow conveyer belt 10 bottom is penetrated into after shower nozzle spraying sterilization of liquids or cleaning seed, in first shower nozzle 2, in 70% ethanol of ejection, the 3rd shower nozzle 4,10% hydrogenperoxide steam generator of ejection, the 5th shower nozzle 6 spray 0.1M mercuric chloride solution reusable edible, adjust line speed so that seed by the time that first shower nozzle the 2, second shower nozzle the 3, the 3rd shower nozzle the 4, the 4th shower nozzle the 5, the 5th shower nozzle the 6, the 6th shower nozzle 7 sprays be respectively 1,1,5,1,5,5min.
Every seed can be separated by automatic particle counter 8, and accurately measures the quantity of seed, and rotating and culturing dish 9 is rotatable so that seed is distributed in rotating and culturing dish 9 uniformly.
Embodiment 2
nullThe Plantula Brassicae chinensis seed soaking 24h is put in charging aperture 1,Open the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、6th shower nozzle 7、Conveyer belt 10、Automatic particle counter 8、Rotating and culturing dish 9,Adjust line speed,Make seed by the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、The time of the 6th shower nozzle 7 spray is respectively 1、1、5、1、5、5min,Adjust the velocity of rotation of rotating and culturing dish 9,Make sterilized Plantula Brassicae chinensis seed uniform and scattered be distributed on rotating and culturing dish 9,Randomly selecting 20 seeds uses beef extract-peptone culture method to carry out picking up bacterium,Separately take 100 seeds and calculate germination percentage,Result shows 20 seed sterilizings the most completely,Germination percentage is 93%,Show that the method is while sterilizing complete to Plantula Brassicae chinensis,Do not significantly reduce the germination percentage of Plantula Brassicae chinensis.
Embodiment 3
nullThe wheat seed soaking 24h is put in charging aperture 1,Open the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、6th shower nozzle 7、Conveyer belt 10、Automatic particle counter 8、Rotating and culturing dish 9,Adjust line speed,Make seed by the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、The time of the 6th shower nozzle 7 spray is respectively 1、1、5、1、5、5min,Adjust the velocity of rotation of rotating and culturing dish 9,Make sterilized wheat seed uniform and scattered be distributed on rotating and culturing dish 9,Randomly selecting 20 seeds uses beef extract-peptone culture method to carry out picking up bacterium,Separately take 100 seeds and calculate germination percentage,Result shows 20 seed sterilizings the most completely,Germination percentage is 95%,Show that the method is while sterilizing complete to Semen Tritici aestivi,Do not significantly reduce the germination percentage of Semen Tritici aestivi.
Embodiment 4
nullThe Semen Brassicae campestris soaking 24h is put in charging aperture 1,Open the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、6th shower nozzle 7、Conveyer belt 10、Automatic particle counter 8、Rotating and culturing dish 9,Adjust line speed,Make seed by the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、The time of the 6th shower nozzle 7 spray is respectively 1、1、5、1、5、5min,Adjust the velocity of rotation of rotating and culturing dish 9,Make sterilized Semen Brassicae campestris uniform and scattered be distributed on rotating and culturing dish 9,Randomly selecting 20 seeds uses beef extract-peptone culture method to carry out picking up bacterium,Separately take 100 seeds and calculate germination percentage,Result shows 20 seed sterilizings the most completely,Germination percentage is 97%,Show that the method is while sterilizing complete to Brassica campestris L,Do not significantly reduce the germination percentage of Brassica campestris L.
Embodiment 5
nullThe cucumber seeds soaking 24h is put in charging aperture 1,Open the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、6th shower nozzle 7、Conveyer belt 10、Automatic particle counter 8、Rotating and culturing dish 9,Adjust line speed,Make seed by the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、The time of the 6th shower nozzle 7 spray is respectively 1、1、5、1、5、5min,Adjust the velocity of rotation of rotating and culturing dish 9,Make sterilized cucumber seeds uniform and scattered be distributed on rotating and culturing dish 9,Randomly selecting 20 seeds uses beef extract-peptone culture method to carry out picking up bacterium,Separately take 100 seeds and calculate germination percentage,Result shows 20 seed sterilizings the most completely,Germination percentage is 91%,Show that the method is in the effect that Fructus Cucumidis sativi can play complete sterilizing,But corn germination rate is had a certain impact.
Embodiment 6
nullThe corn seed soaking 24h is put in charging aperture 1,Open the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、6th shower nozzle 7、Conveyer belt 10、Automatic particle counter 8、Rotating and culturing dish 9,Adjust line speed,Make seed by the first shower nozzle 2、Second shower nozzle 3、3rd shower nozzle 4、4th shower nozzle 5、5th shower nozzle 6、The time of the 6th shower nozzle 7 spray is respectively 1、1、5、1、5、5min,Adjust the velocity of rotation of rotating and culturing dish 9,Make sterilized corn seed uniform and scattered be distributed on rotating and culturing dish 9,Randomly selecting 20 seeds uses beef extract-peptone culture method to carry out picking up bacterium,Separately take 100 seeds and calculate germination percentage,Result shows that in 20 seeds, complete sterilization rate is 42%,Germination percentage is 90%,Show that the method can not build such to the Semen Maydis complete sterilizing of bigger seed,Therefore the use of this device should be confined on less seed.
Claims (3)
1. one kind simple aseptic group training device, it is characterised in that by charging aperture (1), the first shower nozzle (2), the second shower nozzle (3),
3rd shower nozzle (4), the 4th shower nozzle (5), the 5th shower nozzle (6), the 6th shower nozzle (7), automatic particle counter (8), rotating and culturing
Dish (9), conveyer belt (10) form;First shower nozzle (2), the second shower nozzle (3), the 3rd shower nozzle (4), the 4th shower nozzle (5),
5th shower nozzle (6), the 6th shower nozzle (7) are positioned at conveyer belt (10) top, and charging aperture (1) is positioned at conveyer belt (10) one end
Top, automatic particle counter (8) is positioned at the bottom of conveyer belt (10) other end so that the communicated band of sterilizing seed falls into automatically
In several instrument (8), rotating and culturing dish (9) is positioned at automatic particle counter (8) lower left quarter, in automatic particle counter (8) single fall
Under seed be evenly distributed in rotating and culturing dish (9).
The simple aseptic group of training device of one the most according to claim 1, it is characterised in that shower nozzle length ratio is: the first spray
Head (2) length: the second shower nozzle (3) length: the 3rd shower nozzle (4) length: the 4th shower nozzle (5) length: the 5th shower nozzle (6)
Length: the 6th shower nozzle (7) length=1:1:5:1:5:5.
The simple aseptic group of training device of one the most according to claim 1, it is characterised in that conveyer belt (10) is netted knot
Structure.
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CN201620142478.2U CN205596815U (en) | 2016-02-25 | 2016-02-25 | Simple and easy aseptic group banks up device with earth |
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CN201620142478.2U CN205596815U (en) | 2016-02-25 | 2016-02-25 | Simple and easy aseptic group banks up device with earth |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN116114596A (en) * | 2022-09-07 | 2023-05-16 | 济宁市林业保护和发展服务中心 | Culture medium for early maturing pear seedling and seedling device thereof |
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2016
- 2016-02-25 CN CN201620142478.2U patent/CN205596815U/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN116114596A (en) * | 2022-09-07 | 2023-05-16 | 济宁市林业保护和发展服务中心 | Culture medium for early maturing pear seedling and seedling device thereof |
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