CN205528721U - Preparation and sterilization apparatus of sterile culture base - Google Patents
Preparation and sterilization apparatus of sterile culture base Download PDFInfo
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- CN205528721U CN205528721U CN201620363792.3U CN201620363792U CN205528721U CN 205528721 U CN205528721 U CN 205528721U CN 201620363792 U CN201620363792 U CN 201620363792U CN 205528721 U CN205528721 U CN 205528721U
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- circulating line
- culture medium
- preparation
- compound tank
- interface
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- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 230000001954 sterilising effect Effects 0.000 title abstract description 19
- 238000004659 sterilization and disinfection Methods 0.000 title abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000005303 weighing Methods 0.000 claims abstract description 9
- 230000000844 anti-bacterial effect Effects 0.000 claims description 15
- 230000017531 blood circulation Effects 0.000 claims description 7
- 239000008213 purified water Substances 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 11
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 238000013019 agitation Methods 0.000 description 4
- 230000004087 circulation Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Preparation and sterilization apparatus of sterile culture base belongs to microbial culture equipment technical field. Preparation and sterilization apparatus of sterile culture base, can be effectual the working strength of the configuration of reduction culture medium and sterilization, reduce the quality risk among sterilization and the the transfer process. Two one end of heat exchanger water inlet and circulating line intercommunication, two other ends of circulating line feed through with the interface no. 2 of the tee bend valves of CIPSIP system, three one end of dehvery pump output and circulating line intercommunication, three other ends of circulating line feed through with the interface no. 1 of the tee bend valves of CIPSIP system, the cushion flask feeds through through the hose with the interface no. 3 of the tee bend valves of CIPSIP system, weighing module installs at batching tank bottom end, installs a plurality of instrument and a plurality of valve on the batching jar. The utility model discloses can effectively reduce the configuration and the sterilization working strength of culture medium, reduce the quality risk among sterilization and the the transfer process, operation and control process all are easily understood.
Description
Technical field
This utility model belongs to microbial cultivation equipment technical field, particularly relates to preparation and the bactericidal unit of a kind of aseptic culture medium.
Background technology
The preparation of existing culture medium, sterilizing and fill all use the container of tub or other forms, typically first culture medium is dissolved in container, in being placed on moist heat sterilization cabinet after dissolving, carry out sterilizing, but in the process, container is affected by steriliser performance, pressure in container cavity produces change, the phenomenon that sterilized culture medium is sprayed easily occurs, simultaneously, due to off-line sterilizing, during media transfer, easily cause the risk of pollution, affect culture medium serviceability.
Summary of the invention
The problem existed for above-mentioned prior art, this utility model provides preparation and the bactericidal unit of a kind of aseptic culture medium, it is possible to effectively reduces culture medium configuration and the working strength of sterilizing, reduces the quality risk in sterilizing and transport process.
Technical solution adopted in the utility model is: the preparation of aseptic aseptic culture medium and bactericidal unit, including material-compound tank；The preparation of described aseptic culture medium and bactericidal unit also include culture medium blood circulation, Weighing module, the three-way valve group of CIP/SIP system, multiple instrument and multiple valve, and described culture medium blood circulation includes material-compound tank, heat exchanger, surge flask, dehvery pump, circulating line one, circulating line two, circulating line three and circulating line four；Connected by circulating line one between described material-compound tank upper end and heat exchanger outlet, material-compound tank lower end is connected by circulating line four with dehvery pump input, described heat exchanger water inlet connects with circulating line two one end, described circulating line two other end connects with the interface two of the three-way valve group of CIP/SIP system, described dehvery pump outfan connects with circulating line three one end, described circulating line three other end connects with the interface one of the three-way valve group of CIP/SIP system, described surge flask passes through soft pipe connection with the interface three of the three-way valve group of CIP/SIP system, described Weighing module is arranged on material-compound tank bottom, multiple instrument and multiple valve are installed on material-compound tank.
The beneficial effects of the utility model are:
This utility model uses culture medium blood circulation to complete culture medium configuration and the process of sterilizing, by this kind of mode, can effectively reduce configuration and the sterilizing works intensity of culture medium, reduce the quality risk in sterilizing and transport process, operate and control process is all easily understood.
Accompanying drawing explanation
Fig. 1: this utility model flow chart；
The A portion enlarged drawing of Fig. 2: Fig. 1；
The B portion enlarged drawing of Fig. 3: Fig. 1；
The C portion enlarged drawing of Fig. 4: Fig. 1；
Wherein: 1-material-compound tank；2-dehvery pump；3-Weighing module；The three-way valve group of 4-CIP/SIP system；4-1-interface one, 4-2-interface two, 4-3-interface three, 5-heat exchanger；6-surge flask；7-valve；8-instrument；9-chuck；10-flexible pipe；11-circulating line one；12-circulating line two；13-circulating line three；14-circulating line four.
Detailed description of the invention
As shown in Fig. 1 ~ Fig. 4, the preparation of aseptic culture medium and bactericidal unit, including material-compound tank 1；The preparation of described aseptic culture medium and bactericidal unit also include culture medium blood circulation, Weighing module 3, the three-way valve group 4 of CIP/SIP system, multiple instrument 8 and multiple valve 7, and described culture medium blood circulation includes material-compound tank 1, heat exchanger 5, surge flask 6, dehvery pump 2, circulating line 1, circulating line 2 12, circulating line 3 13 and circulating line 4 14；Connected by circulating line 1 between described material-compound tank 1 upper end and heat exchanger 5 outlet, material-compound tank 1 lower end is connected by circulating line 4 14 with dehvery pump 2 input, described heat exchanger 5 water inlet connects with circulating line 2 12 one end, described circulating line 2 12 other end connects with interface two 4-2 of the three-way valve group 4 of CIP/SIP system, described dehvery pump 2 outfan connects with circulating line 3 13 one end, described circulating line 3 13 other end connects with interface one 4-1 of the three-way valve group 4 of CIP/SIP system, described surge flask 6 is connected by flexible pipe 10 with interface three 4-3 of the three-way valve group 4 of CIP/SIP system, described Weighing module 3 is arranged on material-compound tank 1 bottom, multiple instrument 8 and multiple valve 7 are installed on material-compound tank 1.
The preparation of aseptic culture medium according to claim 1 and bactericidal unit, it is characterised in that: the plurality of instrument 8 is temperature sensor, pressure transducer and pointer Pressure gauge respectively.
The plurality of valve 7 is purified water valve, spray Ball valve and other public medium valves respectively.
With bactericidal unit, the preparation of described aseptic culture medium also includes that chuck 9, described chuck 9 are sleeved on material-compound tank 1 lateral surface.
On described Agitation Tank 1, respiratory organ is installed.
nullUsing method: the existing entrance input that feeds intake by quantitative culture base-material powder by Agitation Tank 1，And dissolve according to the corresponding purified water of configuration amount addition，The addition of purified water calculates according to the Weighing module 3 that Agitation Tank 1 is tank bottoms，Carry out the purified water in Agitation Tank 1 and culture base-material powder again being sufficiently stirred for dissolving，After dissolving，Open dehvery pump 2，Open the industrial steam mouth of pipe of heat exchanger 5，Open interface one 4-1 and interface two 4-2 of the three-way valve group 4 of CIP/SIP system，Close interface three 4-3 of the three-way valve group 4 of CIP/SIP system，The culture medium of circulation in pipeline is heated up，After temperature rises to 121 DEG C，Keep circulation in 30 minutes (showing the record time by temperature)，So that culture medium is carried out sufficient sterilizing，After sterilization process terminates，Open the cooling switch of heat exchanger 5，Culture medium in chuck 9 and pipeline is cooled down，Keep culture medium to circulate in pipeline simultaneously，Cool the temperature to close after 50 DEG C the cooling switch of heat exchanger 5，Open interface three 4-3 of the three-way valve group 4 of CIP/SIP system，Close interface two 4-2 of the three-way valve group 4 of CIP/SIP system，Prepare fill，In pouring process，Chuck 9 is utilized to be incubated，Its culture medium temperature is made to be maintained at about 50 DEG C，Dehvery pump 2 is according to surge flask 6 liquid level，It is turned on and off，After fill terminates，Close interface three 4-3 of the three-way valve group 4 of CIP/SIP system，Open interface two 4-2 of the three-way valve group 4 of CIP/SIP system，Open the purified water valve in multiple valve 7，Material-compound tank 1 is carried out，After material-compound tank 1 cleans，Material-compound tank 1 tank level is maintained at tank body 1/3rd，Open dehvery pump 2 and be circulated flushing.I.e. complete the whole preparation of culture medium, sterilizing, cleaning procedure.
Claims (4)
1. the preparation of aseptic culture medium and a bactericidal unit, including material-compound tank (1)；It is characterized in that: the preparation of described aseptic culture medium and bactericidal unit also include culture medium blood circulation, Weighing module (3), the three-way valve group (4) of CIP/SIP system, multiple instrument (8) and multiple valve (7), and described culture medium blood circulation includes material-compound tank (1), heat exchanger (5), surge flask (6), dehvery pump (2), circulating line one (11), circulating line two (12), circulating line three (13) and circulating line four (14)；nullConnected by circulating line one (11) between described material-compound tank (1) upper end with heat exchanger (5) outlet，Material-compound tank (1) lower end is connected by circulating line four (14) with dehvery pump (2) input，Described heat exchanger (5) water inlet connects with circulating line two (12) one end，Described circulating line two (12) other end connects with the interface two (4-2) of the three-way valve group (4) of CIP/SIP system，Described dehvery pump (2) outfan connects with circulating line three (13) one end，Described circulating line three (13) other end connects with the interface one (4-1) of the three-way valve group (4) of CIP/SIP system，Described surge flask (6) is connected by flexible pipe (10) with the interface three (4-3) of the three-way valve group (4) of CIP/SIP system，Described Weighing module (3) is arranged on material-compound tank (1) bottom，Multiple instrument (8) and multiple valve (7) are installed on material-compound tank (1).
The preparation of aseptic culture medium the most according to claim 1 and bactericidal unit, it is characterised in that: the plurality of instrument (8) is temperature sensor, pressure transducer and pointer Pressure gauge respectively.
The preparation of aseptic culture medium the most according to claim 1 and bactericidal unit, it is characterised in that: the plurality of valve (7) is purified water valve, spray Ball valve and other public medium valves respectively.
4. according to preparation and the bactericidal unit of the aseptic culture medium described in claim 1,2 or 3, it is characterised in that: with bactericidal unit, the preparation of described aseptic culture medium also includes that chuck (9), described chuck (9) are sleeved on material-compound tank (1) lateral surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620363792.3U CN205528721U (en) | 2016-04-27 | 2016-04-27 | Preparation and sterilization apparatus of sterile culture base |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620363792.3U CN205528721U (en) | 2016-04-27 | 2016-04-27 | Preparation and sterilization apparatus of sterile culture base |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205528721U true CN205528721U (en) | 2016-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620363792.3U Active CN205528721U (en) | 2016-04-27 | 2016-04-27 | Preparation and sterilization apparatus of sterile culture base |
Country Status (1)
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| CN (1) | CN205528721U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116215929A (en) * | 2022-09-07 | 2023-06-06 | 河北雅果食品有限公司 | Aseptic discharging device and on-line detection control system |
-
2016
- 2016-04-27 CN CN201620363792.3U patent/CN205528721U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116215929A (en) * | 2022-09-07 | 2023-06-06 | 河北雅果食品有限公司 | Aseptic discharging device and on-line detection control system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |