CN204989021U - Detection apparatus for aflatoxins in grain product - Google Patents
Detection apparatus for aflatoxins in grain product Download PDFInfo
- Publication number
- CN204989021U CN204989021U CN201520601861.5U CN201520601861U CN204989021U CN 204989021 U CN204989021 U CN 204989021U CN 201520601861 U CN201520601861 U CN 201520601861U CN 204989021 U CN204989021 U CN 204989021U
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- Prior art keywords
- pipeline
- aflatoxins
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- unit
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- 229930195730 Aflatoxin Natural products 0.000 title claims abstract description 28
- 239000005409 aflatoxin Substances 0.000 title claims abstract description 28
- 235000011868 grain product Nutrition 0.000 title claims abstract description 14
- 238000001514 detection method Methods 0.000 title abstract description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 15
- 150000002170 ethers Chemical class 0.000 claims abstract description 5
- 230000003287 optical effect Effects 0.000 claims description 10
- 238000004891 communication Methods 0.000 claims description 3
- 238000013329 compounding Methods 0.000 abstract 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 239000000523 sample Substances 0.000 description 14
- 235000013305 food Nutrition 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 2
- 206010007269 Carcinogenicity Diseases 0.000 description 2
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 2
- 229930020125 aflatoxin-B1 Natural products 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000007670 carcinogenicity Effects 0.000 description 2
- 231100000260 carcinogenicity Toxicity 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000010183 spectrum analysis Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- PFNQVRZLDWYSCW-UHFFFAOYSA-N (fluoren-9-ylideneamino) n-naphthalen-1-ylcarbamate Chemical compound C12=CC=CC=C2C2=CC=CC=C2C1=NOC(=O)NC1=CC=CC2=CC=CC=C12 PFNQVRZLDWYSCW-UHFFFAOYSA-N 0.000 description 1
- 102100034212 AFG1-like ATPase Human genes 0.000 description 1
- 102100034213 ATPase family protein 2 homolog Human genes 0.000 description 1
- 101100434480 Arabidopsis thaliana AFB2 gene Proteins 0.000 description 1
- 101100449517 Arabidopsis thaliana GRH1 gene Proteins 0.000 description 1
- 101000780581 Homo sapiens AFG1-like ATPase Proteins 0.000 description 1
- 101000780587 Homo sapiens ATPase family protein 2 homolog Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 101100434479 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) AFB1 gene Proteins 0.000 description 1
- 239000002115 aflatoxin B1 Substances 0.000 description 1
- WWSYXEZEXMQWHT-WNWIJWBNSA-N aflatoxin B2 Chemical compound C=1([C@@H]2CCO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O WWSYXEZEXMQWHT-WNWIJWBNSA-N 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model relates to a detection apparatus for aflatoxins in grain product, including rubbing crusher (1), rubbing crusher connects compounding jar (3) through first pipeline (2), the compounding jar is installed on water bath constant temperature oscillator (4), the compounding jar passes through second pipeline (5) and connects first storage tank (6), second storage tank (8) is still connected through third pipeline (7) to the compounding jar, be equipped with among both one kind of ethers solution or methyl alcohol in the first storage tank, the another kind of setting in the second storage tank, detection device (10) is still connected through fourth pipeline (9) to the compounding jar, all be equipped with force pump (11) on all pipelines, still include controlling means, force pump (11) on controlling means control connection rubbing crusher (1), water bath constant temperature oscillator (4) and all pipelines. The utility model discloses an advantage: this device simple structure, it is with low costs, detect fastly, it is accurate to detect the structure, can satisfy the demand in enterprise and market.
Description
Technical field
The utility model relates to a kind of device for detecting safety of foods, particularly the pick-up unit of aflatoxins in a kind of cereal product.
Background technology
Along with the raising further of people's living standard, people more and more focus on the safety of food under the prerequisite of having enough to eat and wear meeting, the aflatoxins of one of major pollutants in cereal product more and more receives the concern of people.Aflatoxins is a kind of molecule mycotoxin, and be naturally occurring hypertoxicity, strong carcinogenicity material, be extensively present in the grain agricultural product gone mouldy, the grain agricultural product of countries in the world are generally subject to the pollution of aflatoxin.Aflatoxins known at present and derivant thereof have kind more than 20, i.e. B1 (AFB1), B2 (AFB2), G1 (AFG1), G2 (AFG2) etc. 20 kinds.The harmfulness of aflatoxin is there is destruction to people and animal's liver tissue, and liver cancer can be caused time serious even dead.The most common with aflatoxin B1 in the food of natural contamination, its toxicity and carcinogenicity are also the strongest, have great importance so aflatoxins detects in time, fast and efficiently to the safety of food.
The instrument of the current detection to aflatoxins of China is all expensive, detects comparatively consuming time, cannot reach the demand in market far away.
Utility model content
The purpose of this utility model is that the instrument of detection in order to solve present stage aflatoxins is all expensive, detects comparatively consuming time, cannot reach the shortcomings such as the demand in market far away, and the pick-up unit of aflatoxins in a kind of cereal product proposed.
To achieve these goals, the utility model have employed following technical scheme:
The pick-up unit of aflatoxins in a kind of cereal product, it is characterized in that: comprise comminutor, described comminutor connects mixing tank by the first pipeline, described mixing tank is arranged on described water-bath constant temperature oscillator, described mixing tank connects the first storage tank by second pipe, described mixing tank also connects the second storage tank by the 3rd pipeline, the one in both ethers solution or methyl alcohol is provided with in described first storage tank, another kind is arranged in described second storage tank, described mixing tank also connects pick-up unit by the 4th pipeline, at described first pipeline, second pipe, 3rd pipeline and the 4th pipeline are equipped with forcing pump,
Described pick-up unit comprises casing, connection fixing base in described casing, be connected successively optical filter and Laser Focusing sheet in described holder, photodiode is connected in the upside of described holder, sample stage is connected by jacking gear in the downside of holder, also at least be provided with in the downside of holder one with the ultraviolet LED of described sample stage corresponding matching, the light that ultraviolet LED sends irradiates photodiode pass described Laser Focusing sheet and optical filter successively after described sample stage reflection after, described casing is also provided with control display screen, control display screen communication and connect described photodiode,
Also comprise control device, the forcing pump on comminutor, water-bath constant temperature oscillator and all pipelines described in control device control linkage.
On the basis of technique scheme, following further technical scheme can be had:
Described comminutor adopts swing comminutor.
Described casing is provided with case lid, is provided with cylinder in the side of casing, the piston rod of described cylinder is arranged straight up, and the lower end of cylinder is hinged on hinged seat, the piston rod of cylinder and the side of described case lid hinged fixing.
Described holder is tubular structure, in holder, be provided with through hole, and be connected described optical filter and Laser Focusing sheet in through hole, is provided with flange in the side of described holder.
Described ultraviolet LED is connected to the downside of described holder by support.
Described support is the cover platform structure of a taper.
The utility model has the advantage of: this apparatus structure is simple, and cost is low, detection speed is fast, and detection architecture is accurate, can meet the demand in enterprise and market.
Accompanying drawing explanation
Fig. 1 is basic structure schematic diagram of the present utility model.
Embodiment
In order to make the utility model clearly understand, describe in detail this device below in conjunction with accompanying drawing, specific embodiment described herein only in order to explain the utility model, and is not used in restriction the utility model.
As shown in Figure 1, in a kind of cereal product that the utility model provides, the pick-up unit of aflatoxins, is characterized in that: comprise comminutor 1, and described comminutor 1 is prior art, and described comminutor 1 adopts swing comminutor.In comminutor 1, be provided with motor, motor drives worm screw and worm gear drive mechanism by belt and belt pulley, is driven roll structure by worm screw and worm gear drive mechanism.
Described comminutor 1 connects mixing tank 3 by the first pipeline 2, and described mixing tank 3 is arranged on described water-bath constant temperature oscillator 4, and described water-bath constant temperature oscillator 4 is prior art, mixes by water-bath constant temperature oscillator 4 making the material in mixing tank 3.Described mixing tank 3 connects the first storage tank 6 by second pipe 5, described mixing tank 3 also connects the second storage tank 8 by the 3rd pipeline 7, described first storage tank 6 and the second storage tank 8 can be two independently storage tanks, also can be that a storage tank is provided with interlayer in centre, described interlayer be divided into two independently point chambeies.In described first storage tank 6, be provided with the one in both ethers solution or methyl alcohol, another kind is arranged in described second storage tank 8, and described methyl alcohol adopts 50ml, and described ethers solution adopts sherwood oil, and its capacity adopts 20ml.Described mixing tank 3 also connects pick-up unit 10 by the 4th pipeline 9, and described first pipeline 2, second pipe 5, the 3rd pipeline 7 and the 4th pipeline 9 are equipped with forcing pump 11.
Also comprise control device, the forcing pump 11 on comminutor 1, water-bath constant temperature oscillator 4 and all pipelines described in control device control linkage.
Described pick-up unit 10 comprises the casing 12 of a rectangular structure, described casing 12 is provided with case lid 21, described case lid 21 is sealed and matched with casing 12, cylinder 22 is provided with in the side of casing 12, the piston rod of described cylinder 22 is arranged straight up, the lower end of cylinder 22 is hinged on hinged seat 23, and the piston rod of cylinder 22 is hinged fixing by the side of freely movable hinge and described case lid 21.
A fixed head is provided with in described casing 12, a center pit is provided with in fixed head, be connected a holder 13 vertically arranged in described center pit, described holder 13 is tubular structure, through hole 13a is provided with in holder 13, be connected described optical filter 14 and Laser Focusing sheet 15 in through hole 13a, is provided with flange 13b in the side of described holder 13.
Photodiode 16 is connected in the upside of described holder 13, sample stage 18 is connected by jacking gear 17 in the downside of holder 13, described jacking gear 17 adopts electric pushrod, described sample stage 18 is circular discs, be connected in the downside of described casing 12 striker plate 26, center pit is provided with, described sample stage 18 and described center pit corresponding matching in the centre of described striker plate 26.
Connect one group of ultraviolet LED19 in the downside of holder 13 by support 24, described support 24 is the cover platform structure of a taper.All ultraviolet LED19 are all corresponding irradiates described sample stage 18, the light that ultraviolet LED19 sends irradiates photodiode 16 through after described Laser Focusing sheet 15 and optical filter 14 successively after described sample stage 18 reflects, described casing 12 is also provided with and controls display screen 20, control display screen 20 communication and connect described photodiode 16, described display screen 20 is provided with control knob 25.
The model of described ultraviolet LED19 is TH-UV365S1E-5, and the distribution range of its spectrum is 365-375nm, and centre wavelength is 370nm, and diameter is 5nm, and emission angle is 100-1200, direct current 200ma, and forward operating voltage is 3.2-3.8V.
This ultraviolet LED is as transmitting illuminant, and it is fine that its centre wavelength and fluorescence excitation coincide, the features such as this product also has wavelength standard simultaneously, energy is high, light decay is low, the life-span is long, light efficiency is good, no color differnece.Multiple ultraviolet LED distributes in the mode of circle by this detecting device in space, under the control of MCU, launches Ultraviolet radiation with certain intensity of illumination and frequency and be detected sample.
The Main Function of described Laser Focusing sheet 15 converts fluorescence signal to electric signal.The fluorescence that aflatoxins is sent and fluorescent probe realize rationally and perfectly mate, and need to carry out necessary process to fluorescence signal.The efficiency being converted to fluorescence due to the ultraviolet hitting aflatoxins only has about 18 &.So want to obtain the higher fluorescence of relative intensity, laser focusing lens should be taked to assemble its fluorescence.
By analysis, this aflatoxins detecting device adopts BL-2.0 laser focusing lens.Its diameter is 20mm, and focal length is 50.8mm, and adopt domestic superfine CVD zinc selenide, its shape is crescent shape.Can bear high power density, superficial film is firm, and difficult drop-off, reduces or eliminates optical surface veiling glare, effectively increases assembly transmittance.The transmittance of this laser focusing lens can reach about 98%.
After the gathering of Laser Focusing sheet 15, scattered light and impurity light is also there is in fluorescence light path, the spectrum that these impurity light produces can have influence on the quality of aflatoxins spectrum, so try every possible means to be filtered out, these just can accurately ensure the correctness of aflatoxins spectral analysis.
Obtaining ultraviolet cutoff wavelength the longest after consulting relevant data is 400mm, so the cutoff wavelength selecting glass filter is 400mm, in addition, also in order to ensure the accuracy of aflatoxins spectral analysis, the cutoff rate of selected optical filter 14 is more than 99%.
The case lid 21 of casing 12 in use, is opened by device, is placed on by appropriate sample on sample stage 18, closes the case lid 21 of casing 12 and makes control knob 25, then the light that ultraviolet LED19 sends starts transmitting illuminant and irradiates sample.The light allowing ultraviolet LED19 send first irradiates sample a period of time, after light source steadily, makes the gathering of sample reflection light through laser condensing lens and the filtration of glass filter, is finally irradiated on the photodiode by the fluorescence after filtration.Illumination causes the curent change of photodiode, so just light signal can be transformed into electric signal, is finally utilizing described display screen 20 and relevant program to analyse and compare to the spectrum that it produces, and determines that whether it is containing aflatoxins.
Claims (6)
1. the pick-up unit of aflatoxins in a cereal product, it is characterized in that: comprise comminutor (1), described comminutor (1) connects mixing tank (3) by the first pipeline (2), described mixing tank (3) is arranged on described water-bath constant temperature oscillator (4), described mixing tank (3) connects the first storage tank (6) by second pipe (5), described mixing tank (3) also connects the second storage tank (8) by the 3rd pipeline (7), the one in both ethers solution or methyl alcohol is provided with in described first storage tank (6), another kind is arranged in described second storage tank (8), described mixing tank (3) also connects pick-up unit (10) by the 4th pipeline (9), described first pipeline (2), second pipe (5), 3rd pipeline (7) and the 4th pipeline (9) are equipped with forcing pump (11),
Described pick-up unit (10) comprises casing (12), connection fixing base (13) in described casing (12), be connected successively optical filter (14) and Laser Focusing sheet (15) in described holder (13), photodiode (16) is connected in the upside of described holder (13), sample stage (18) is connected by jacking gear (17) in the downside of holder (13), also at least be provided with in the downside of holder (13) one with the ultraviolet LED(19 of described sample stage (18) corresponding matching), ultraviolet LED(19) light that sends irradiates photodiode (16) afterwards through described Laser Focusing sheet (15) and optical filter (14) successively after described sample stage (18) reflection, described casing (12) is also provided with and controls display screen (20), control display screen (20) communication and connect described photodiode (16),
Also comprise control device, the forcing pump (11) on comminutor (1), water-bath constant temperature oscillator (4) and all pipelines described in control device control linkage.
2. the pick-up unit of aflatoxins in a kind of cereal product according to claim 1, is characterized in that: described comminutor (1) adopts swing comminutor.
3. the pick-up unit of aflatoxins in a kind of cereal product according to claim 1, it is characterized in that: on described casing (12), be provided with case lid (21), cylinder (22) is provided with in the side of casing (12), the piston rod of described cylinder (22) is arranged straight up, the lower end of cylinder (22) is hinged on hinged seat (23), the piston rod of cylinder (22) and the side of described case lid (21) hinged fixing.
4. the pick-up unit of aflatoxins in a kind of cereal product according to claim 1, it is characterized in that: described holder (13) is tubular structure, through hole (13a) is provided with in holder (13), be connected described optical filter (14) and Laser Focusing sheet (15) in through hole (13a), is provided with flange (13b) in the side of described holder (13).
5. the pick-up unit of aflatoxins in a kind of cereal product according to claim 1, is characterized in that: described ultraviolet LED(19) downside of described holder (13) is connected to by support (24).
6. the pick-up unit of aflatoxins in a kind of cereal product according to claim 5, is characterized in that: described support (24) is the cover platform structure of a taper.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201520601861.5U CN204989021U (en) | 2015-08-12 | 2015-08-12 | Detection apparatus for aflatoxins in grain product |
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CN201520601861.5U CN204989021U (en) | 2015-08-12 | 2015-08-12 | Detection apparatus for aflatoxins in grain product |
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CN201520601861.5U Expired - Fee Related CN204989021U (en) | 2015-08-12 | 2015-08-12 | Detection apparatus for aflatoxins in grain product |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105044068A (en) * | 2015-08-12 | 2015-11-11 | 孟令启 | Device for detecting aflatoxin in grain product |
CN113884362A (en) * | 2021-11-08 | 2022-01-04 | 浙江科技学院 | Kit for detecting mycotoxin in grains |
-
2015
- 2015-08-12 CN CN201520601861.5U patent/CN204989021U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105044068A (en) * | 2015-08-12 | 2015-11-11 | 孟令启 | Device for detecting aflatoxin in grain product |
CN113884362A (en) * | 2021-11-08 | 2022-01-04 | 浙江科技学院 | Kit for detecting mycotoxin in grains |
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Legal Events
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170124 Address after: East Donghua road 233100 Anhui Longzihu district city of Bengbu province No. 9 Patentee after: Anhui Science and Technology University Address before: High tech Zone in Anhui city of Bengbu Province Xinghua Road 233010 No. 168 Patentee before: Meng Lingqi |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160120 Termination date: 20160812 |