CN204918596U - Primary hepatocyte separation system comprising liver tissue fixing and multi-layer filtering device - Google Patents
Primary hepatocyte separation system comprising liver tissue fixing and multi-layer filtering device Download PDFInfo
- Publication number
- CN204918596U CN204918596U CN201520519168.3U CN201520519168U CN204918596U CN 204918596 U CN204918596 U CN 204918596U CN 201520519168 U CN201520519168 U CN 201520519168U CN 204918596 U CN204918596 U CN 204918596U
- Authority
- CN
- China
- Prior art keywords
- screen cloth
- portable plate
- hepatic tissue
- plate
- filtering device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001914 filtration Methods 0.000 title claims abstract description 32
- 210000003494 hepatocyte Anatomy 0.000 title claims abstract description 28
- 238000000926 separation method Methods 0.000 title claims abstract description 11
- 210000005228 liver tissue Anatomy 0.000 title abstract 6
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 23
- 239000011324 bead Substances 0.000 claims abstract description 15
- 210000004027 cell Anatomy 0.000 claims abstract description 9
- 230000002440 hepatic effect Effects 0.000 claims description 58
- 239000004744 fabric Substances 0.000 claims description 44
- 238000009434 installation Methods 0.000 claims description 28
- 238000003760 magnetic stirring Methods 0.000 claims description 9
- 239000011796 hollow space material Substances 0.000 claims description 8
- 230000010412 perfusion Effects 0.000 abstract description 14
- 210000001519 tissue Anatomy 0.000 description 54
- 102000029816 Collagenase Human genes 0.000 description 12
- 108060005980 Collagenase Proteins 0.000 description 12
- 229960002424 collagenase Drugs 0.000 description 12
- 238000000034 method Methods 0.000 description 6
- 210000005229 liver cell Anatomy 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 210000000887 face Anatomy 0.000 description 3
- 208000019423 liver disease Diseases 0.000 description 3
- 206010019799 Hepatitis viral Diseases 0.000 description 2
- 206010073310 Occupational exposures Diseases 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 231100000675 occupational exposure Toxicity 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 201000001862 viral hepatitis Diseases 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 210000000795 conjunctiva Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002324 hematogenic effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Landscapes
- External Artificial Organs (AREA)
Abstract
The utility model discloses a primary hepatocyte separation system containing a liver tissue fixing and multi-layer filtering device, which consists of a liver tissue fixing device, a multi-layer filtering device, a magnetic stirrer, a liquid storage container and a peristaltic pump, wherein the liver tissue fixing device and the multi-layer filtering device are sequentially connected with a first peristaltic pump, a liquid storage container and a second peristaltic pump through pipelines, the liver tissue fixing device is in a hollow cubic configuration, and the size of the hollow cubic shape is adjustable; the multilayer filtering device is divided into an end cover part, a middle part and a bottom part which are connected through threads, screens with different apertures are arranged in each part, anti-blocking magnetic beads are arranged, primary hepatic cells with high purity can be obtained through filtering perfusion liquid containing cells layer by layer, and the perfusion liquid can be recycled. The utility model provides a complete set of perfect device system of separating primary hepatocyte from liver tissue of structure, simple and convenient, swift, the higher primary hepatocyte of economic acquisition purity.
Description
Technical field
The utility model relates to field of biomedicine technology, and specifically a kind of hepatic tissue that comprises is fixed and the primary hepatocyte separation system of multilayer filtering device.
Background technology
Collagenase perfusion method is primary hepatocyte separation method main at present.This method carries out collagenase perfusion by existing vascular system to bulk hepatic tissue, or fritter hepatic tissue is carried out to the method acquisition primary hepatocytes such as multi-point lancet puncture collagenase perfusion.The hepatocyte suspension be separated tentatively screens out larger tissue block, manadesma etc. by filter screen, then adopts the method for density gradient centrifugation to obtain purer liver cell.Current China viral hepatitis, pathogenesis of cirrhosis rate still occupy high-order, and Metabolic liver disease sickness rate rises year by year, and hepatic diseases is still the principal disease posed a health risk, and research and the demand for the treatment of for primary hepatocyte of hepatic diseases increase day by day.Particularly bioartificial liver's R&D work and clinical application possible in the future, need the primary hepatocyte that a large amount of purity is higher.
And current domestic employing collagenase perfusion obtains the working method of primary hepatocyte, lack the perfusion equipment of whole set of system, have the following disadvantages in operation: 1, hepatic tissue is without proprietary stationary installation, artificial hand fix fritter hepatic tissue carry out the puncture of multidigit point time, have and the risk of pricking wound occur.Particularly be engaged in scientific research, during the Hematogenic infectious disease patient liver organization primary hepatocytes such as isolated viral hepatitis, if there is pricking wound occupational exposure, operator can be being caused to infect corresponding pathogenic agent; 2, hepatic tissue is without proprietary stationary installation, and in collagenase perfusion process, the situation of hepatic tissue displacement, the disengaging of perfusion shower nozzle, liquid splash easily occurs, and these liquid splashes, to positions such as eye conjunctivas, mucous membrane occupational exposure can occur; 3, lack multilayer filtration system, need complicated density gradient centrifugation could obtain the higher primary hepatocyte of purity; And block for preventing, the perfusate containing collagenase can not recycle.
Utility model content
The purpose of this utility model is to provide a kind of hepatic tissue that comprises to fix and the primary hepatocyte separation system of multilayer filtering device, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the utility model provides following technical scheme:
Comprise hepatic tissue to fix and the primary hepatocyte separation system of multilayer filtering device, be made up of hepatic tissue stationary installation, multilayer filtering device, magnetic stirring apparatus, the first peristaltic pump, liquid storage container and the second peristaltic pump, described hepatic tissue stationary installation is arranged in multilayer filtering device, connects the first peristaltic pump, liquid storage container and the second peristaltic pump successively between hepatic tissue stationary installation and multilayer filtering device by pipeline;
The hollow square body structure that described hepatic tissue stationary installation is made up of multiple retaining plate and portable plate, described retaining plate and portable plate all have sieve aperture, it is all stiff that described retaining plate comprises the first retaining plate, the second retaining plate and the 3rd retaining plate that are closely connected, and the first retaining plate, the second retaining plate and the 3rd retaining plate form cubical three fixed faces jointly; Described portable plate comprises the first portable plate and the second portable plate, first portable plate is slided on the second pulley track by the first pulley, second portable plate is slided on the first pulley track by the second pulley, first portable plate is provided with the equal intersection seam in multiple interval, one end of intersection seam is closed and is connected with the first portable plate main body, the other end of intersection seam opens and holds the second portable plate and passes, and the first portable plate and the second portable plate form cubical two active face; Second retaining plate and the second portable plate all offer the fixed orifices between sieve aperture, be equipped with fixed plate in fixed orifices, multiple fixed plate penetrates after in fixed orifices and forms a cubical face; Described first pulley and the second pulley are equipped with thumbwheel switch;
Described multi-layer filtrating equipment is made up of the end cap portions of hollow cylindrical, pars intermedia and bottom three part, cubical hollow space is provided with in described end cap portions, and hollow space and hepatic tissue stationary installation are coincide, the interior lower end of end cap portions is provided with the first screen cloth, the lower end of end cap portions is also provided with end cap portions screw thread, the top of described pars intermedia is provided with the threaded upper ends be connected with end cap portions screw thread, and end cap portions screw thread and threaded upper ends form the first threaded connection place, the inside of described pars intermedia is provided with the second screen cloth, the space that first screen cloth and the second screen cloth are formed is cell mass receiving cavity, described second screen cloth is placed with the first magnetic bead, the lower end of described pars intermedia is provided with lower end screw thread, the top of described bottom is provided with the bottom thread be connected with lower end screw thread, lower end screw thread and bottom thread form the second threaded connection place, the inside of described bottom is provided with the 3rd screen cloth, the space that second screen cloth and the 3rd screen cloth are formed is cell enrichment cavity, described 3rd screen cloth is placed with the second magnetic bead, the space, bottom being positioned at the 3rd screen cloth is the temporary storage chamber body of perfusate, the sidewall of described bottom is provided with liquid exit.
As further program of the utility model: the aperture of described first screen cloth, the second screen cloth and the 3rd screen cloth reduces successively.
Compared with prior art, the beneficial effects of the utility model are:
1, the utility model provides the apparatus system being separated primary hepatocyte from hepatic tissue of a whole set of structure improvement, the primary hepatocyte that easy, quick, economic acquisition purity is higher;
2, can hepatic tissue be fixed, avoid free-hand fixing hepatic tissue, during multi-point lancet puncture, pricking wound occur;
3, fixing hepatic tissue, avoids hepatic tissue to be subjected to displacement, causes perfusate to splash;
4, there is multilayer filtration system, the collagenase perfusate after filtration, perfusion can be repeated in hepatic tissue, the consumption of collagenase can be reduced, cost-saving.
Accompanying drawing explanation
Fig. 1 is enforcement schematic diagram of the present utility model.
Fig. 2 is the structural representation of hepatic tissue stationary installation in the utility model.
Fig. 3 is the cross sectional representation of the first retaining plate in hepatic tissue stationary installation, the 3rd retaining plate;
Fig. 4 is the cross sectional representation of the 3rd retaining plate, the second portable plate in hepatic tissue stationary installation;
Fig. 5 is the cross-sectional structure schematic diagram of fixed plate in hepatic tissue stationary installation;
Fig. 6 is the cross sectional representation of the first portable plate in hepatic tissue stationary installation;
Fig. 7 is the Split type structure schematic diagram of multilayer filtering device in the utility model.
Fig. 8 is the one-piece construction schematic diagram of multilayer filtering device in the utility model.
In figure: 1-first retaining plate; 2-fixed orifices; 3-second pulley track; 4-first pulley; 5-second retaining plate; 6-hepatic tissue stationary installation; 7-the 3rd retaining plate; 8-second portable plate; 9-first portable plate; 10-intersects seam; 11-first pulley track; 12-second pulley; 13-sieve aperture; 14-fixed plate; 15-hollow space; 16-end cap portions; 17-first screen cloth; 18-end cap portions screw thread; 19-threaded upper ends; 20-pars intermedia; 21-second screen cloth; 22-lower end screw thread; 23-first magnetic bead; 24-bottom thread; Bottom 25-; 26-the 3rd screen cloth; 27-liquid exit; The temporary storage chamber body of 28-perfusate; 29-second magnetic bead; 30-first threaded connection place; 31-cell mass receiving cavity; 32-second threaded connection place; 33-cell enrichment cavity; 34-magnetic stirring apparatus; 35-magnetic stirring apparatus speed governing button; 36-multilayer filtering device; 37-hepatic tissue; 38-first peristaltic pump; 39-liquid storage container; 40-second peristaltic pump.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
Refer to Fig. 1 ~ 8, in the utility model embodiment, comprise hepatic tissue to fix and the primary hepatocyte separation system of multilayer filtering device, be made up of hepatic tissue stationary installation, multilayer filtering device, magnetic stirring apparatus, the first peristaltic pump, liquid storage container and the second peristaltic pump, it is characterized in that, described hepatic tissue stationary installation is arranged in multilayer filtering device, connects the first peristaltic pump, liquid storage container and the second peristaltic pump successively between hepatic tissue stationary installation and multilayer filtering device by pipeline.
The hollow square body structure that hepatic tissue stationary installation 6 is made up of multiple retaining plate and portable plate, retaining plate, portable plate and fixed plate are made up of metallic substance, stainless steel or polycarbonate; Retaining plate and portable plate all have sieve aperture 13, described retaining plate comprise the first retaining plate 1, second retaining plate 5 of being closely connected and the 3rd retaining plate 7 all stiff, the first retaining plate 1, second retaining plate 5 and the 3rd retaining plate 7 form cubical three fixed faces jointly; Portable plate comprises the first portable plate 9 and the second portable plate 8, first portable plate 9 is slided on the second pulley track 3 by the first pulley 4, second portable plate 8 is slided on the first pulley track 11 by the second pulley 12, first portable plate 9 is provided with the equal intersection seam 10 in multiple interval, one end of intersection seam 10 is closed and is connected with the first portable plate 9 main body, the other end of intersection seam 10 opens and holds the second portable plate 8 and passes, and the first portable plate 9 and the second portable plate 8 form cubical two active face; Second retaining plate 5 and the second portable plate 8 all offer the fixed orifices 2 between sieve aperture, be equipped with fixed plate 14 in fixed orifices 2, multiple fixed plate penetrates after in fixed orifices 2 and forms a cubical face.
First pulley 4 and the second pulley 12 are equipped with thumbwheel switch, after moving to required position, locking thumbwheel switch, portable plate is fixed on retaining plate, by the collocation of retaining plate and portable plate, and according to hepatic tissue size, realize the expansion of cubic space and reduce, better to fix hepatic tissue.
Hepatic tissue stationary installation 6 is the quantity adjustable of the cubes configuration of hollow adjustable size, retaining plate wherein and portable plate.For this stationary installation retaining plate and the adjustment of portable plate quantity, the hepatic tissue stationary installation forming the adjustable hollow cube configuration of size is all considered as the protection domain of the utility model patent.
Multi-layer filtrating equipment 36 is made up of the end cap portions 16 of hollow cylindrical, pars intermedia 20 and bottom 25 3 part, and the shell of multi-layer filtrating equipment is made up of transparent polycarbonate material, can observe the height of liquid storage tank and inner chamber liquid level easily; This device total height is 10cm, and except end cap portions 16, the diameter of interior hollow-cylindrical is 8cm, and cubic capacity is about 500ml.
Cubical hollow space 15 is provided with in end cap portions 16, and hollow space 15 coincide with hepatic tissue stationary installation 6, the interior lower end of end cap portions 16 is provided with the first screen cloth 17, first screen cloth 17 is 20 orders, its aperture 0.8mm, relatively large hepatic tissue chip and manadesma etc. can be filtered, and allow little liver cell agglomerate to pass through; The lower end of end cap portions 16 is also provided with end cap portions screw thread 18, and the top of pars intermedia 20 is provided with the threaded upper ends 19 be connected with end cap portions screw thread 18, and end cap portions screw thread 18 and threaded upper ends 19 form the first threaded connection place 30; It is 300 orders that the inside of pars intermedia 20 is provided with the second screen cloth 21, second screen cloth 21, and 50 μm, its aperture, allows unicellular size and less material to pass through; The space that first screen cloth 17 and the second screen cloth 21 are formed is cell mass receiving cavity 31.
Second screen cloth 21 is placed with the first magnetic bead 23, when magnetic stirring apparatus 34 works, first magnetic bead 23 rotarily drives the motion of liquid vortex sample, blocks and promotes the abundant effect that collagenase is rolled into a ball liver cell, make it become single primary hepatocyte to prevent the second screen cloth 21; The lower end of pars intermedia 20 is provided with lower end screw thread 22, and the top of bottom 25 is provided with the bottom thread 24 be connected with lower end screw thread 22, and lower end screw thread 22 and bottom thread 24 form the second threaded connection place 32.
To be provided with the 3rd screen cloth the 26, three screen cloth 26 be 1250 orders in the inside of bottom 25,10 μm, its aperture, unicellular not by; The space that second screen cloth 21 and the 3rd screen cloth 26 are formed is cell enrichment cavity the 33, three screen cloth 26 is placed with the second magnetic bead 29, second magnetic bead 29 to rotarily drive liquid vortex sample and move, and blocks to prevent the second screen cloth 26; The space, bottom 25 being positioned at the 3rd screen cloth 26 is the temporary storage chamber body 28 of perfusate, and the sidewall of bottom is provided with liquid exit 27.
Above-mentioned comprise hepatic tissue fix and the primary hepatocyte separation system of multilayer filtering device as follows for separating of the concrete steps of hepatic tissue cell:
(1) hepatic tissue is fixed
First carry out sterilising treatment to each parts of reactor, each step of isolating hepatocytes is all carried out in aseptic super clean bench or hundred grades of laboratories, is placed on by hepatic tissue in space that three retaining plates surround; Second portable plate 8 is installed, slides the second portable plate 8 until compress hepatic tissue, then locking first pulley 4 along the first pulley track 11; First portable plate 9 being installed, making the second portable plate 8 at correct position through intersecting seam 10, slide the first portable plate 9 until compression hepatic tissue, then locking second pulley 12 along the second pulley track 2; Be inserted through fixed plate in fixed orifices 2 on the second portable plate 8 and the second retaining plate 5, thus formed six faces cubes stationary installation for be fixed for cellular segregation hepatic tissue;
(2) assembling of system
By good by corresponding helical assembly to end cap portions 16, pars intermedia 20 and bottom 25, and place the first magnetic bead 23 and the second magnetic bead 29, to form multilayer filtering device 36; The hepatic tissue stationary installation 6 being fixed with hepatic tissue is placed in hollow space 15, and liquid line connects peristaltic pump, magnetic stirring apparatus 34 and liquid storage container 39;
(3) hepatic tissue collagenase perfusate perfusion
Perfusion shower nozzle inserts in hepatic tissue vascular by sieve aperture 13, or thrust in hepatic tissue by puncture needle multi-point, open the second peristaltic pump 40, by collagenase perfusate perfusion in liquid storage container 39 in hepatic tissue, liver cell under the effect of collagenase in hepatic tissue is separated gradually, and with perfusate toward dirty, when multilayer filtering device 36 liquid level is more than the first mesh screen 17 height, open magnetic stirring apparatus 34 adjusting rotary speed, make the first magnetic bead 23 and the second magnetic bead 29 slow circumvolve, and open the first peristaltic pump 38 immediately, with corresponding first peristaltic pump 38 corresponding speed the perfusate in temporary for perfusate storage chamber body 28 discharged by liquid exit 27 and be back in liquid storage container 39, in perfusing course, fixed plate 14 can be removed, and help liver cell fully to dissociate by blade cuts,
(4) primary hepatocyte is collected
The second peristaltic pump 40 is stopped at the end of perfusion, first peristaltic pump 38 works on, perfusate in suction multilayer filtering device 36, when perfusate liquid level stops suction lower than during the second threaded connection place 32 height, and stop magnetic stirring apparatus 34 to work, turn on by screw thread and be separated pars intermedia 20 and bottom 25, with pipettor transfer primary hepatocyte suspension in cell therefor, with further centrifugal acquisition primary hepatocyte.
To those skilled in the art, obvious the utility model is not limited to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present utility model or essential characteristic, can realize the utility model in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present utility model is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the utility model.Any Reference numeral in claim should be considered as the claim involved by limiting.
Claims (2)
1. comprise hepatic tissue to fix and the primary hepatocyte separation system of multilayer filtering device, be made up of hepatic tissue stationary installation (6), multilayer filtering device (36), magnetic stirring apparatus (34), the first peristaltic pump (38), liquid storage container (39) and the second peristaltic pump (40), it is characterized in that, described hepatic tissue stationary installation (6) is arranged in multilayer filtering device (36), connects the first peristaltic pump (38), liquid storage container (39) and the second peristaltic pump (40) successively between hepatic tissue stationary installation (6) and multilayer filtering device (36) by pipeline;
The hollow square body structure that described hepatic tissue stationary installation (6) is made up of multiple retaining plate and portable plate, described retaining plate and portable plate all have sieve aperture (13), it is all stiff that described retaining plate comprises the first retaining plate (1), the second retaining plate (5) and the 3rd retaining plate (7) that are closely connected, and the first retaining plate (1), the second retaining plate (5) and the 3rd retaining plate (7) form cubical three fixed faces jointly, described portable plate comprises the first portable plate (9) and the second portable plate (8), first portable plate (9) is above slided at the second pulley track (3) by the first pulley (4), second portable plate (8) is above slided at the first pulley track (11) by the second pulley (12), first portable plate (9) is provided with equal intersection seam (10) in multiple interval, one end of intersection seam (10) is closed and is connected with the first portable plate (9) main body, the other end of intersection seam (10) opens and holds the second portable plate (8) and passes, first portable plate (9) and the second portable plate (8) form cubical two active face, second retaining plate (5) and the second portable plate (8) all offer the fixed orifices (2) be positioned between sieve aperture (13), is equipped with fixed plate (14) in fixed orifices (2), multiple fixed plate penetrates after in fixed orifices (2) and forms a cubical face, described first pulley (4) and the second pulley (12) are equipped with thumbwheel switch,
Described multi-layer filtrating equipment (36) is by the end cap portions (16) of hollow cylindrical, pars intermedia (20) and bottom (25) three part composition, cubical hollow space (15) is provided with in described end cap portions (16), and hollow space (15) coincide with hepatic tissue stationary installation (6), the interior lower end of end cap portions (16) is provided with the first screen cloth (17), the lower end of end cap portions (16) is also provided with end cap portions screw thread (18), the top of described pars intermedia (20) is provided with the threaded upper ends (19) be connected with end cap portions screw thread (18), end cap portions screw thread (18) and threaded upper ends (19) form the first threaded connection place (30), the inside of described pars intermedia (20) is provided with the second screen cloth (21), the space that first screen cloth (17) and the second screen cloth (21) are formed is cell mass receiving cavity (31), described second screen cloth (21) is placed with the first magnetic bead (23), the lower end of described pars intermedia (20) is provided with lower end screw thread (22), the top of described bottom (25) is provided with the bottom thread (24) be connected with lower end screw thread (22), lower end screw thread (22) and bottom thread (24) form the second threaded connection place (32), the inside of described bottom (25) is provided with the 3rd screen cloth (26), the space that second screen cloth (21) and the 3rd screen cloth (26) are formed is cell enrichment cavity (33), described 3rd screen cloth (26) is placed with the second magnetic bead (29), bottom (25) space being positioned at the 3rd screen cloth (26) is the temporary storage chamber body (28) of perfusate, the sidewall of described bottom is provided with liquid exit (27).
2. the hepatic tissue that comprises according to claim 1 is fixed and the primary hepatocyte separation system of multilayer filtering device, it is characterized in that, the aperture of described first screen cloth (17), the second screen cloth (21) and the 3rd screen cloth (26) reduces successively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520519168.3U CN204918596U (en) | 2015-07-16 | 2015-07-16 | Primary hepatocyte separation system comprising liver tissue fixing and multi-layer filtering device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520519168.3U CN204918596U (en) | 2015-07-16 | 2015-07-16 | Primary hepatocyte separation system comprising liver tissue fixing and multi-layer filtering device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204918596U true CN204918596U (en) | 2015-12-30 |
Family
ID=54967742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520519168.3U Expired - Fee Related CN204918596U (en) | 2015-07-16 | 2015-07-16 | Primary hepatocyte separation system comprising liver tissue fixing and multi-layer filtering device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204918596U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928176A (en) * | 2015-07-16 | 2015-09-23 | 中南大学湘雅医院 | Primary hepatocyte separation system and method containing tissue fixation and multi-layer filtration device |
-
2015
- 2015-07-16 CN CN201520519168.3U patent/CN204918596U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928176A (en) * | 2015-07-16 | 2015-09-23 | 中南大学湘雅医院 | Primary hepatocyte separation system and method containing tissue fixation and multi-layer filtration device |
| CN104928176B (en) * | 2015-07-16 | 2017-03-08 | 中南大学湘雅医院 | Primary hepatocyte separation system and method containing tissue fixation and multi-layer filtration device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204043972U (en) | A kind of to granulated samples carrying out the chemical detection liquid extraction tube of lixiviate | |
| CN104928176A (en) | Primary hepatocyte separation system and method containing tissue fixation and multi-layer filtration device | |
| CN204918596U (en) | Primary hepatocyte separation system comprising liver tissue fixing and multi-layer filtering device | |
| CN209716027U (en) | A kind of Biohazard Waste Disposal grinding device | |
| CN209854150U (en) | Novel dynamic early embryo culture dish with culture solution updated in real time | |
| CN201197915Y (en) | Split type filtering funnel | |
| CN210543745U (en) | Sample purification device for drug residue analysis | |
| CN218893669U (en) | Placenta mesenchymal stem cell separator | |
| CN206069861U (en) | A kind of centrifuge tube for separating plant intercellular washing fluids | |
| CN208076251U (en) | A kind of clinical laboratory's sample processing apparatus | |
| CN208695175U (en) | A kind of push type placenta cutter device | |
| CN210448755U (en) | Purification and filtration device for extracellular vesicles | |
| CN203852641U (en) | Fat blood filter | |
| CN109566603B (en) | Visceral system specimen storage device | |
| CN202015539U (en) | High-accuracy horizontal filter | |
| CN201411458Y (en) | Device used for collecting microalgae | |
| CN205473751U (en) | Cord blood stem cells isolated device | |
| CN108497505A (en) | A kind of decompression spinach juice extraction equipment | |
| CN2865698Y (en) | Fat cleaning purifier | |
| JP5606693B2 (en) | Liquid oil component removal method, cell separation method and cell separation kit | |
| CN217661757U (en) | Drainage device with filtering function | |
| CN218980665U (en) | Filtering device applied to drug synthesis processing | |
| CN218710410U (en) | Sorting and filtering device for tumor cell mass for oncology department | |
| CN213537950U (en) | Stem cell ball cutting and collecting device | |
| CN213113315U (en) | Animal tissue broken liquid component separating tube |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151230 |