CN204907445U - Group with wide -mouth glass bottle and centrifuging tube preparation banks up bottle with earth - Google Patents

Abstract

The utility model discloses a tissue culture bottle made of a wide-mouth glass bottle and a centrifuge tube, which comprises a glass bottle body, a bottle cap, a straight centrifuge column, a thermometer casing, a sterilization indicating tape or an annotation label, and a scale scale. The upper part of the glass bottle body is covered with a bottle cap, and there are two holes on the bottle cap. A thermometer sleeve for placing a thermometer is installed in one of the holes of the bottle cap; a centrifuge tube with a tapered bottom is installed in the other hole of the bottle cap. The centrifuge tube is filled with a breathable cotton plug to form a straight centrifuge column with air-permeable bacteria isolation. The side wall of the glass bottle is bonded with a scale scale with double-sided tape, and there will be a sterilization instruction tape or an annotation label on the bottle cap. It has the characteristics of simple operation, economical and practical.

Description

A kind of tissue culture bottle made of wide-mouth glass bottle and centrifuge tube

technical field

The utility model belongs to the technical field of plant cultivation, in particular to a tissue culture bottle made of wide-mouth glass bottles and centrifuge tubes.

Background technique

Traditional plant tissue culture techniques are mainly carried out in plant tissue culture flasks, including the processes of callus culture, induction and differentiation. During this period, the requirements for plant tissue culture bottles were very high, especially for the materials of bottle caps and tissue culture bottles, which must meet the oxygen required for plant growth without causing pollution. At present, there are two main types of plant tissue culture bottles on the market, one is a polyethylene plastic bottle with breathable sterile filtration, and the other is a white transparent glass bottle with a polyethylene bottle cap. The common feature is that there is a microporous filter membrane with a diameter of 2cm on the bottle cap or a tampon column for filter sterilization to ensure the air in the tissue culture bottle. Plant culture experiments using plant tissue culture bottles are mainly carried out in the laboratory, and there will be batch tissue culture experiments with different combination formulas and different culture conditions, so a large number of labeling experiments are required. The current practice is mainly in the organization Paste the glued label paper on the culture bottle or directly write the mark on the surface of the bottle with an oil-based marker. No matter the label paper or the oil-based marker pen, it will fall off or the color will dissolve and fade during the sterilization process, which will cause certain damage to the test. mess.

At present, there are a lot of waste glass products in the garbage recycling stations on the market, especially white flat-bottomed glass jars of different specifications, and many discarded centrifuge tubes of different specifications are produced in the laboratory every day, and there is a lot of waste. and environmental pollution.

Utility model content

The utility model aims to overcome the defects of the prior art. The utility model provides a tissue culture bottle made of wide-mouth glass bottles and centrifuge tubes. Tissue culture bottle, not only has a graduated scale, but also has an antibacterial filter gas column, a thermometer sleeve, and a sterilization indicator label and a marking pen to mark the position. It is multifunctional and economical and practical.

The technical scheme of the utility model is as follows:

A tissue culture bottle made of wide-mouth glass bottles and centrifuge tubes, including a glass bottle body, a bottle cap, a straight centrifuge column, a thermometer sleeve, a sterilization indicating tape or an annotation label, a scale scale, and the upper cover of the glass bottle body There are two holes on the bottle cap, and a thermometer casing for placing a thermometer is installed in one hole of the bottle cap; a centrifuge tube with a tapered bottom is installed in the other hole of the bottle cap, and the centrifuge tube is filled with breathable cotton The stopper forms a straight centrifuge column with air-permeable bacteria isolation. The side wall of the glass bottle is bonded with a scale scale with double-sided tape, and there will be a sterilization indicating tape or an annotation label on the bottle cap.

Preferably, the bottle body is a round glass bottle with a flat bottom.

Preferably, the bottle cap is made of light iron, and the bottle cap has internal threads and a thin layer of silicone gasket.

Preferably, the bottle body and the bottle cap are fastened by a spiral thread.

Preferably, the sealing between the centrifuge column and the bottle cap, and between the thermowell and the bottle cap is bonded with a high-temperature-resistant adhesive.

Preferably, the scale scale is a scale scale capable of sterilization and high temperature resistance.

Compared with the prior art, the utility model has the following beneficial effects:

It can make full use of discarded glass bottles and centrifuge tubes to make plant tissue culture bottles suitable for laboratory operations. It has the functions of temperature measurement, plant height measurement, sterilization marks and annotation marks.

Description of drawings

Figure 1 is a schematic diagram of the overall structure of the utility model.

Detailed ways

The preferred embodiments of the present utility model are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present utility model, and are not intended to limit the present utility model.

As shown in Figure 1, a tissue culture bottle made of a wide-mouth glass bottle and a centrifuge tube, including a glass bottle body 1, a bottle cap 2, a straight centrifuge column 3, a thermometer sleeve 4, a sterilization indicator tape or notes Label 5, scale scale 6, the upper part of the glass bottle body 1 is screwed and fastened to cover the bottle cap 2, the bottle body is a white, flat bottom, round glass bottle with good light transmission, the bottle cap is made of light iron, and the bottle cap With internal thread and thin silicone gasket. There are two holes on the bottle cap 2, and a thermometer casing 4 for placing a thermometer is installed in one of the holes of the bottle cap; a centrifuge tube with a tapered bottom is installed in the other hole of the bottle cap, and the centrifuge tube is filled with a breathable tampon to form The air-permeable bacteria-isolated straight centrifuge column 3 is sealed with a high-temperature-resistant adhesive between the centrifuge column and the bottle cap, and between the thermometer sleeve and the bottle cap. The side wall of the bottle body 1 is bonded with a scale scale 6 by double-sided adhesive, and the scale scale is a scale scale capable of sterilization and high temperature resistance. There will be a sterilization indicating tape or an annotation label 5 pasted on the bottle cap. After sterilization, the sterilization indicating tape will change color, and the color changing tape will be used as a place marked by a marker pen.

The production method is to pick a white flat-bottomed transparent glass bottle body 1 with a diameter of 7cm and a height of 10cm, and use double-sided adhesive to bond the bottle body with a high temperature resistant scale scale 6, remove the bottle cap 2, and use a hole puncher with a diameter of 0.5cm Punch two holes on the bottle cap with a distance of 1cm; take two 1.5mL centrifuge tubes (0.5cm in diameter and 4cm in height) discarded in the laboratory, and install one directly in the small hole of the bottle cap to form a 4 sets of centrifuge tubes with a round bottom or conical bottom for the thermometer; use a knife to cut off the conical part of the bottom of the other centrifuge tube, leaving about 3.0 cm in the upper section, fill with a breathable cotton plug, and install it on the bottle cap after filling. In the hole, a straight centrifuge column 3 is formed with a breathable bacteria-proof tampon, and the seal between the two centrifuge columns and the bottle cap is bonded with a high-temperature-resistant adhesive (there is a brand-name black must-seal adhesive meter). Prepare 30 mL of MS solid medium in a culture bottle, cover the bottle cap, paste the sterilization indicator tape 5 on the bottle cap, and perform 121°C sterilization treatment. After the sterilization is completed, inoculate the plant tissue, and sterilize Mark on the instruction label. Insert a thermometer into the thermowell and incubate.

The above is only to illustrate the implementation of the utility model, and is not intended to limit the utility model. For those skilled in the art, any modification, equivalent replacement, Improvements and the like should all be included within the protection scope of the present utility model.

Claims (6)

1. A tissue culture bottle made of wide-mouth glass bottle and centrifuge tube, comprising glass bottle body, bottle cap, straight centrifuge column, thermometer sleeve, sterilization indicating tape or annotation label, scale scale, is characterized in that : The upper part of the glass bottle body is covered with a bottle cap, and there are two holes on the bottle cap, and a thermometer sleeve for placing a thermometer is installed in one of the holes of the bottle cap; a centrifuge tube with a tapered bottom is installed in the other hole of the bottle cap , The centrifuge tube is filled with a breathable cotton plug to form a straight centrifuge column with air-permeable bacteria isolation. The side wall of the glass bottle is bonded with a scale scale with double-sided tape, and there will be a sterilization instruction tape or an annotation label on the bottle cap. 2. a kind of tissue culture bottle made with wide-mouth glass bottle and centrifuge tube according to claim 1, is characterized in that, glass bottle body is a flat-bottomed cylindrical glass bottle. 3. a kind of tissue culture bottle made with wide-mouth glass bottle and centrifuge tube according to claim 1, is characterized in that, bottle cap is light iron product, and bottle cap has internal thread and thin-layer silica gel sealing gasket. 4. a kind of tissue culture bottle made with wide-mouth glass bottle and centrifuge tube according to claim 1, is characterized in that, adopts helical thread fastening between glass bottle body and bottle cap. 5. a kind of tissue culture bottle made with wide-mouth glass bottle and centrifuge tube according to claim 1 is characterized in that, between the centrifuge column and the bottle cap, between the thermometer casing and the bottle cap, sealing adopts high temperature resistant adhesive Adhesive bonding. 6. a kind of tissue culture bottle made with wide-mouth glass bottle and centrifuge tube according to claim 1, is characterized in that, scale scale is the scale scale that can be sterilized and resistant to high temperature.