CN204509510U - The device of continuous production biochip - Google Patents

The device of continuous production biochip Download PDF

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Publication number
CN204509510U
CN204509510U CN201520073432.5U CN201520073432U CN204509510U CN 204509510 U CN204509510 U CN 204509510U CN 201520073432 U CN201520073432 U CN 201520073432U CN 204509510 U CN204509510 U CN 204509510U
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China
Prior art keywords
shower nozzle
nozzle
biochip
sampling point
sample
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Expired - Fee Related
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CN201520073432.5U
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Chinese (zh)
Inventor
蔡锦达
许智
姚尚金
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University of Shanghai for Science and Technology
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University of Shanghai for Science and Technology
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Abstract

The device of continuous production biochip, comprising: cylinder, is used for carrying biochip matrix membrane, wherein biochip matrix membrane is distributed with multiple sampling point; Drum drive, is connected with cylinder, be used for head roll rotate; Shower nozzle, shower nozzle has multiple nozzle be arranged in a linear, and each nozzle correspondence arranges a piezoquartz switch, by piezoquartz on-off control nozzle ejection liquid; Shower nozzle driving mechanism, is connected with shower nozzle, is used for driving shower nozzle by rectilinear movement; Capacity transducer, for detecting the Level Detection of sample liquid in shower nozzle, when the little alarm of sample liquid; And controller, be connected with drum drive and shower nozzle driving mechanism, can index drum and shower nozzle by predetermined speed relative movement.Utilize the device of continuous production biochip of the present utility model, the continuous point sample of biological sample can be realized, increase substantially point sample efficiency, shorten the preparation time of biochip.

Description

The device of continuous production biochip
Technical field
The utility model relates to the device of continuous production biochip.
Background technology
Biochip technology is current medical science, biological field front-end product, mainly exists with the form of biological sample microarray.By adopting biochip point sample instrument (or also can be called " spot sample device ") by biological sample as DNA, RNA, protein and other biological composition are fixed on the solid phase compact substance carriers such as silicon chip, sheet glass or pottery sheet by matrix dot form with high-density and high-precision mode, form biological micro-array.
Existing point sample instrument adopts piezo jets to carry out spray printing sample static state to prepare biochip mostly.Piezo jets exists many nozzles, each nozzle is all independently control to realize need based jet by corresponding piezoquartz.The drop volume ejected due to piezo jets single-nozzle quite little (5 ~ 80pL level), and the volume of sampling point comparatively large (10 ~ 15nL level) on biochip, therefore the general mode adopted is static spray sample, namely static after moving to the position of point sample, the liquid droplets of nozzle for several times, complete the preparation of this lot sample point.After preparation, shower nozzle moves to next batch sampling point place again, sprays preparation for several times by respective nozzle.This kind of mode seriously constrains the point sample efficiency of biochip.In addition, due to processing problems, differ in piezo jets upper spray nozzle aperture, therefore adopts above-mentioned static spray printing mode, easily causes and prepare the decline of sampling point consistence, affect later stage research work.
Utility model content
The purpose of this utility model is to provide a kind of continuity point sampling device, significantly to shorten biochip preparation time, and improves the consistence of sampling point to a certain extent.
According to the device of continuous production biochip of the present utility model, comprising:
Cylinder, is used for carrying biochip matrix membrane, wherein biochip matrix membrane is distributed with multiple sampling point;
Drum drive, is connected with cylinder, be used for head roll rotate;
Shower nozzle, shower nozzle has multiple nozzle be arranged in a linear, and each nozzle correspondence arranges a piezoquartz switch, by piezoquartz on-off control nozzle ejection liquid;
Shower nozzle driving mechanism, is connected with shower nozzle, is used for driving shower nozzle by rectilinear movement;
Capacity transducer, for detecting the Level Detection of sample liquid in shower nozzle, when the little alarm of sample liquid; And
Controller, is connected with drum drive and shower nozzle driving mechanism, can index drum and shower nozzle by predetermined speed relative movement.
In a preferred embodiment of the present utility model, wherein shower nozzle is multiple, and multiple shower nozzle can form circumference array shower nozzle along cylinder circumferential array, the spacing P of adjacent shower nozzle shower nozzlefor the spacing P of sampling point sampling pointintegral multiple; Multiple shower nozzle can carry out point sample to multiple sampling point simultaneously.
Traditional point sample instrument can be avoided for meeting sampling point volume requirement according to spot sample device of the present utility model, need static in the position of point sample, spray state repeatedly, save the time; Further raising point sample efficiency, shortens the preparation time of biochip.Further, due to processing problems, on shower nozzle, differ in different spray nozzles aperture, causes ejected drop volume not of uniform size, causes the biochip sampling point consistence of preparation not high.Adopt transverse shifting shower nozzle to carry out the mode of spray printing point sample, make the sampling point on biochip be all be prepared from by multiple nozzle, even if there is the emitted dose of individual nozzles inconsistent, total sample size there is error average effect, improve sampling point quality.
Accompanying drawing explanation
Fig. 1 is biological chip matrix film schematic diagram;
Fig. 2 is the position relationship side-view of circumference array shower nozzle and cylinder;
Fig. 3 is the position relationship front view of circumference array shower nozzle and cylinder;
Fig. 4 is continuity point sample loading mode schematic diagram.
Embodiment
Describe the device of continuous production biochip of the present utility model below in conjunction with accompanying drawing in detail, it will be appreciated by those skilled in the art that embodiment described below is only to exemplary illustration of the present utility model, but not for making any restriction to it.
According to the device of continuous production biochip of the present utility model, mainly comprise cylinder, drum drive, shower nozzle, shower nozzle driving mechanism, controller and capacity transducer.Cylinder is used for carrying biochip matrix membrane, wherein biochip matrix membrane is distributed with multiple sampling point.Drum drive is connected with cylinder, be used for head roll rotate.Shower nozzle has multiple nozzle be arranged in a linear, the spacing between adjacent nozzle very little (μm level).Each nozzle correspondence arranges a piezoquartz switch, by piezoquartz on-off control nozzle ejection liquid.Shower nozzle driving mechanism is connected with shower nozzle, is used for driving shower nozzle by rectilinear movement.And controller, be connected with drum drive and shower nozzle driving mechanism, can index drum and shower nozzle by predetermined speed relative movement.Capacity transducer for detecting the Level Detection of sample liquid in shower nozzle, when the little alarm of sample liquid.
In the embodiment shown in Fig. 2 and Fig. 3, shower nozzle (5) is for multiple, and multiple shower nozzle (5) can form circumference array Combined nozzle along cylinder (6) circumferential array, the spacing P of adjacent shower nozzle (5) shower nozzlefor the spacing P of sampling point sampling pointintegral multiple.Multiple shower nozzle (5) can carry out point sample to multiple sampling point simultaneously.
Specifically describe the working method of continuous production biochip device of the present utility model below.For convenience of description, prepare sampling point for the single shower nozzle in built-up type sample applying nozzle array, other forms are all similar.
First biochip matrix membrane is fixed on cylinder surface, start-up point sampling device, pre-point sample confirms that each nozzle ejection of shower nozzle is all right, without blocking.See Fig. 1, the upper biochip (2) of biochip matrix membrane (1) in the form of an array ordered arrangement forms; Each biochip (2) is again formed with array ordered arrangement by multiple microarray (3); Single microarray (3) be again by the sampling point of different sample (4) in the form of an array ordered arrangement form.Each shower nozzle (5) has the multiple nozzles along the arrangement of cylinder (6) axial straight line; Needed for a sampling point, volume is V sampling point, the sample drop volume that single-nozzle once ejects is V specking, need the frequency n of spraying at single sampling point place, then n=V sampling point/ V specking, round.
See Fig. 4, the injection of No. i-th nozzle (51) is aimed at a kind of starting point position (a kth sampling point (41)) of sampling point, wherein 1≤i≤s, s is the total quantity of single shower nozzle upper spray nozzle, s >=n.Cylinder (6) uniform rotation, circumference array shower nozzle (5) moving axially along cylinder (6), cylinder (6) is in the whole circle process of turning, No. i-th nozzle (51) to this kind of sampling point all this kind of sampling points (from k (41) to k+m (42)) liquid droplets on biochip matrix membrane (1) in the circumferential, when cylinder (6) turn whole circle forwards the starting point position of this kind of sampling point again to, i-th+j nozzle (52) just in time moves to directly over the starting point position of this kind of sampling point, wherein j be more than or equal to 1 integer, carry out this kind of sampling point second takes turns liquid droplets, spray until the i-th+n-1 nozzle (53) carries out n-th time to this kind of sampling point, prepared by this kind of sampling point.Shower nozzle (5) moves to the starting point position of this kind of sample of next microarray (3) in horizontal direction, repeat above-mentioned spray sample loading mode, until bio-based plasma membrane (1) has been prepared along cylinder (6) all sampling points axially.
Sampling point in theory on same circumferential position matrix membrane is not in fact at same circumferential position.Distance on shower nozzle between adjacent nozzle is d (unit for μm), and first sampling point and last sampling point are δ in the distance that drum shaft upwards staggers, then:
δ=j×d
Owing to being cut into biochip one by one below, then the error that should calculate on biochip is δ 2, matrix membrane is L (unit is mm) along the length in cylinder circumference, and a biochip is L1 (part for L) in the size of matrix membrane length direction.Then:
δ 2=j×d×L 1/L
δ 2extremely little, completely negligible, wherein L=π D 2, D 2for the diameter (unit is mm) of cylinder.Wherein, δ 2accuracy requirement U must be less than, then:
t=U×L/(d×L 1)
T rounds downwards, then j≤t.
When cylinder turns a whole circle, shower nozzle just in time moves j (j >=1) individual injector spacing.The then rotating speed N1 (r/min) of cylinder, the speed of shower nozzle movement is N2 (mm/min).Then must meet following relation between the two:
N 1=j×d×N 2

Claims (2)

1. a device for continuous production biochip, is characterized in that, comprising:
Cylinder, is used for carrying biochip matrix membrane, wherein biochip matrix membrane is distributed with multiple sampling point;
Drum drive, is connected with cylinder, be used for head roll rotate;
Shower nozzle, shower nozzle has multiple nozzle be arranged in a linear, and each nozzle correspondence arranges a piezoquartz switch, by piezoquartz on-off control nozzle ejection liquid;
Shower nozzle driving mechanism, is connected with shower nozzle, is used for driving shower nozzle by rectilinear movement;
Capacity transducer, for detecting the Level Detection of sample liquid in shower nozzle; And
Controller, is connected with drum drive and shower nozzle driving mechanism.
2. the device of continuous production biochip according to claim 1, is characterized in that, wherein shower nozzle is multiple, and multiple shower nozzle can form circumference array shower nozzle along cylinder circumferential array, the spacing P of adjacent shower nozzle shower nozzlefor the spacing P of sampling point sampling pointintegral multiple; Multiple shower nozzle can carry out point sample to multiple sampling point simultaneously.
CN201520073432.5U 2015-02-02 2015-02-02 The device of continuous production biochip Expired - Fee Related CN204509510U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201520073432.5U CN204509510U (en) 2015-02-02 2015-02-02 The device of continuous production biochip

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201520073432.5U CN204509510U (en) 2015-02-02 2015-02-02 The device of continuous production biochip

Publications (1)

Publication Number Publication Date
CN204509510U true CN204509510U (en) 2015-07-29

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Application Number Title Priority Date Filing Date
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CF01 Termination of patent right due to non-payment of annual fee
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Granted publication date: 20150729

Termination date: 20160202