CN204422544U - A kind of TIMP-1, MMP-9 injury of kidney combined detection card - Google Patents
A kind of TIMP-1, MMP-9 injury of kidney combined detection card Download PDFInfo
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- CN204422544U CN204422544U CN201420844398.2U CN201420844398U CN204422544U CN 204422544 U CN204422544 U CN 204422544U CN 201420844398 U CN201420844398 U CN 201420844398U CN 204422544 U CN204422544 U CN 204422544U
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Abstract
The utility model is a kind of TIMP-1, MMP-9 injury of kidney combined detection card, and test card detects human urine matrix metalloproteinase tissue depressant-1 and Matrix Metalloproteinase-9 two kinds of albumen based on collaurum principle.The utility model is got stuck by detection and colloidal gold strip two parts form.Detect in getting stuck and have two grooves for placing the test strip of TIMP-1 and Matrix Metalloproteinase-9 two kinds of albumen.This test strips can realize the content of a step qualitative detection human urine matrix metalloproteinase tissue depressant-1 and Matrix Metalloproteinase-9 two kinds of albumen, has cost low, small and exquisite easy, simple to operate, without the need to features such as professional operator.
Description
Technical field
The utility model belongs to technical field of immune assay, relates to the colloidal-gold detecting-card of injury of kidney mark TIMP-1 (TIMP-1), Matrix Metalloproteinase-9 (MMP-9).
Background technology
There is the population more than 500,000,000 to suffer from kidney trouble in various degree in the world at present, die from the cardiovascular and cerebrovascular disease be associated with chronic kidney disease per year over a population of one million.Morbidity rate in China's Adult Groups is high by 10.8%, about has 1.2 hundred million Patients with Chronic Renal Disease in more than 18 years old crowd.Glomerulosclerosis is the common pathological characters of most of kidney trouble, finally lapsing to of multiple chronic renal disease Progressive symmetric erythrokeratodermia development, be common morphological change in different kidney trouble whole latter stage, cause the destruction of nephron 26S Proteasome Structure and Function, finally develop into chronic renal failure.Renal disease patient is increasing year by year in China, and it is the key measure improving nephrotic's prognosis that early diagnosis is also intervened in time, finds that damage markers contributes to the injury of kidney course of disease and judges.Clinical at present diagnosis and Main Basis serum creatinine and urine volume are by stages carried out to injury of kidney disease, but both this often " damage " before the phase its " danger " phase namely change, timely judgement cannot be made to glomerulosclerosis situation.
Tissue inhibitor of metalloproteinase-1 (tissue inhibitor ofmetalloproteinases 1, TIMP-1) be expressed in a kind of glycoprotein common in tissue, matrix metalloproteinase (MMPs) is a class peptide family of a class degradation of cell epimatrix (ECM).In glomerulosclerosis pathological process, the deposition of ECM plays a significant role, and when MMPs and its tissue inhibiting TIMP-1 function get muddled, plays an important role in glomerulosclerosis development.MMP-9 is one of most important MMPs of human body, and the balance adjusted between MMP-9 and TIMP-1 has become the focus of clinical prevention kidney trouble early, and these two kinds of marks also become the reliabie biomarker of carly fruit drop bead sclerosis.
The utility model develops a kind of colloidal-gold detecting-card simultaneously detecting TIMP-1, MMP-9 two kinds of injury of kidney marks.Collaurum method has simple and quick feature accurately, the utility model utilizes collaurum method to detect TIMP-1, MMP-9 two kinds of marks in human urine, avoid conventional liquid phase chromatography, running time that immunoturbidimetry, chemoluminescence method have is long, need the shortcoming of specialized equipment, only need a step simple operations, testing result can be obtained in 10 minutes.
Utility model content
The one that provides the utility model detects injury of kidney mark TIMP-1, MMP-9 combined detection card simultaneously.Diagnose for adjuvant clinical injury of kidney.
The utility model is by the following technical solutions:
Detect a combined detection card of injury of kidney mark TIMP-1, MMP-9 simultaneously, be made up of detection clamp and draw-in groove two parts.
Clamp gets stuck from above, under to get stuck and TIMP-1, MMP-9 two kinds of injury of kidney marker colloidal gold test strip form.Under get stuck inner side have two grooves for placing colloid gold test paper.On get stuck, have two strip view windows, there are information record label in outside, for indicating that the title of test strips, testing result illustrate and sample message.Two kinds of injury of kidney marker colloidal gold test strip are by backing, sample pad, nitrocellulose filter and adsorptive pads four part composition.Adsorptive pads and sample pad lay respectively at two sections of backing, nitrocellulose filter in the middle part of backing one section with adsorptive pads is overlapping links together, the other end and sample pad is overlapping links together.Nitrocellulose filter is coated with detection line and nature controlling line.Two kinds of injury of kidney marker colloidal gold test strip are put into down in two grooves got stuck respectively, and a sample pad part for two kinds of test strips is exposed and got stuck, nitrocellulose filter and view window one_to_one corresponding.
The groove body of draw-in groove has two holes, respectively corresponding two kinds of colloidal gold strips, and the antibody of colloid gold label is contained in the bottom in hole, corresponding with two kinds of marks respectively.
The utility model colloidal gold strip used all adopts double antibody sandwich method principle to detect corresponding mark, the colloidal gold labeled monoclonal antibody of draw-in groove is mouse injury of kidney mark monoclonal antibody and colloidal gold composite, on nitrocellulose filter, detection line bag is by the mouse injury of kidney mark monoclonal antibody with antibody conjugates on pad, and nature controlling line bag is by sheep anti-mouse igg polyclonal antibody.
During detection, urine specimen is added drop-wise in the sample well of draw-in groove, and the collaurum in sample well is dissolved in sample, then clamp and draw-in groove is covered tightly, and under capillarity, urine is to adsorptive pads direction chromatography.If containing corresponding injury of kidney mark in urine, mark in sample is combined with corresponding golden labeling antibody, then on chromatography to nitrocellulose filter during detection line, be combined with the antibody of bag quilt, form the sandwich structure of " coated antibody-antigen-Jin labeling antibody compound ", and there is red stripes in detection line region, obtain positive findings like this.
When not containing injury of kidney mark in sample, sample and golden labeling antibody and coated antibody all can not react, the gold grain of redness just can not capture by tested survey line when on chromatography to nitrocellulose filter, thus can not demonstrate red stripes in this region, obtains negative findings like this.
Golden labeling antibody on the negative or golden pad of positive sample all can produce red stripes with the many anti-bindings of the sheep anti mouse of nature controlling line on nitrocellulose filter, if explicit declaration testing result is not invalid for nature controlling line.
Adopt injury of kidney mark TIMP-1, MMP-9 combined detection card of such scheme to have following beneficial effect, comprise that cost is low, compact volume and convenience, simple to operate, can detect without the need to professional, and a step can detect two kinds of injury of kidney marks simultaneously.
Accompanying drawing explanation
Fig. 1 front view of the present utility model
Fig. 2 cut-open view of the present utility model
The cut-open view of Fig. 3 injury of kidney marker detection test strips
The cut-open view of Fig. 4 draw-in groove
The testing result schematic diagram of Fig. 5 injury of kidney marker detection test strips
1. get stuck on the 3. sample well 4. test paper title 5. result display window 6. test strips 7. backing 8. adsorptive pads 9. nitrocellulose filter 10. colloidal gold labeled monoclonal antibody compound 11s that get stuck for 2. times. sample pad 12. detection line 13. nature controlling line 14. groove 15. clamp 16. draw-in groove
Embodiment
As depicted in figs. 1 and 2, the utility model is by detecting clamp 15 and draw-in groove 16 forms.Clamp gets stuck 1 from above, under get stuck 2 and injury of kidney marker detection test strips 6 form.On to get stuck inside 1 and under get stuck inside 2 and can combine closely, formed firmly overall.On get stuck outside 1 and have test paper title 4, result display window 5.Test paper title 4 is for illustration of the kind of Test paper, and result display window 5 is corresponding with the nitrocellulose filter 9 of the test strips 6 in groove 14.Under get stuck and 2 have 2 grooves 14, for placing injury of kidney marker detection test strips 6, test strips 6 to put order identical with the order of test paper title 4.
As Fig. 3, colloid gold test paper is made up of backing 7, adsorptive pads 8, nitrocellulose filter 9, sample pad 11, adsorptive pads 8 and sample pad 11 lay respectively at two sections of backing 7, nitrocellulose filter 9 in the middle part of backing 7 one section with adsorptive pads 8 is overlapping links together, the other end and sample pad 11 is overlapping links together.Nitrocellulose filter 9 is coated with detection line 12 and nature controlling line 13.The detection line of two kinds of Test papers wraps by TIMP-1 and MMP-9 antibody respectively, and nature controlling line bag is resisted by sheep anti-mouse igg more, and golden pad wraps respectively by gold mark TIMP-1 and MMP-9 monoclonal antibody.
As Fig. 4, draw-in groove 16 is containing two sample wells 3, and the bottom of sample well 3 is placed with the antibody complex 10 of colloid gold label, the kind of antibody and injury of kidney marker detection test strips 6 one_to_one corresponding.
As Fig. 5, if be positive sample, demonstrate red T line and C line.If be negative sample, then only there is red C line.If there is not red C line, then represent product failure or operation failure, need again detect.
Claims (4)
1. TIMP-1, MMP-9 injury of kidney combined detection card, be made up of detection clamp and draw-in groove two parts, clamp gets stuck from above, under to get stuck and two kinds of TIMP-1, MMP-9P injury of kidney marker colloidal gold test strip form, it is characterized in that: the groove body of draw-in groove has two holes, corresponding TIMP-1, MMP-9 two kinds of colloidal gold strips respectively, the antibody of colloid gold label is contained in the bottom in hole, corresponding with TIMP-1, MMP-9 two kinds of marks respectively.
2. TIMP-1, MMP--9 injury of kidney combined detection card as claimed in claim 1, it is characterized in that: there are two grooves inner side of getting stuck under test card clamp for placing colloid gold test paper, test card clamp gets stuck, and have two strip view windows, there is sample message in outside.TIMP-1, MMP-9 two kinds of injury of kidney marker colloidal gold test strip by backing, sample pad, nitrocellulose filter and adsorptive pads four part composition; Adsorptive pads and sample pad lay respectively at two sections of backing, nitrocellulose filter in the middle part of backing one section with adsorptive pads is overlapping links together, the other end and sample pad is overlapping links together, nitrocellulose filter is coated with detection line and nature controlling line, TIMP-1, MMP-9 two kinds of injury of kidney marker colloidal gold test strip are put into down in two grooves got stuck respectively, a sample pad part for two kinds of test strips is exposed and is got stuck, nitrocellulose filter and view window one_to_one corresponding.
3. TIMP-1, MMP--9 injury of kidney combined detection card as claimed in claim 1, it is characterized in that the detection line of two kinds of Test papers wraps by TIMP-1, MMP-9 antibody respectively, nature controlling line bag is resisted by sheep anti-mouse igg more.
4. TIMP-1, MMP-9 injury of kidney combined detection card as claimed in claim 1, is characterized in that sample well wraps respectively and is marked TIMP-1, MMP-9 injury of kidney mouse resource monoclonal antibody by gold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420844398.2U CN204422544U (en) | 2014-12-29 | 2014-12-29 | A kind of TIMP-1, MMP-9 injury of kidney combined detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420844398.2U CN204422544U (en) | 2014-12-29 | 2014-12-29 | A kind of TIMP-1, MMP-9 injury of kidney combined detection card |
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| Publication Number | Publication Date |
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| CN204422544U true CN204422544U (en) | 2015-06-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420844398.2U Expired - Fee Related CN204422544U (en) | 2014-12-29 | 2014-12-29 | A kind of TIMP-1, MMP-9 injury of kidney combined detection card |
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| CN (1) | CN204422544U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109470848A (en) * | 2018-12-28 | 2019-03-15 | 广州市红十字会医院（暨南大学医学院附属广州红十字会医院） | Detection kit and detection method for myocardial injury markers MMPs, TIMPs and cTnI |
-
2014
- 2014-12-29 CN CN201420844398.2U patent/CN204422544U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109470848A (en) * | 2018-12-28 | 2019-03-15 | 广州市红十字会医院（暨南大学医学院附属广州红十字会医院） | Detection kit and detection method for myocardial injury markers MMPs, TIMPs and cTnI |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160922 Address after: 510000 Guangdong city of Zhuhai province Hengqin Baohua Road No. 6, room 105 -1791 Patentee after: Zhuhai HOPE GENES MEDICINE RESEARCH INSTITUTE CO., LTD. Address before: 102206, B316-15, room 1, 29 life road, Changping District science and Technology Park, Beijing, China Patentee before: Thelma Hopkins Medicine Research Academy (Beijing) Ltd Patentee before: Thelma Hopkins (Beijing) medical diagnosis Science and Technology Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150624 Termination date: 20201229 |
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| CF01 | Termination of patent right due to non-payment of annual fee |