CN204111753U - A kind of high channel nucleic acid extraction purification devices - Google Patents
A kind of high channel nucleic acid extraction purification devices Download PDFInfo
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- CN204111753U CN204111753U CN201420533329.XU CN201420533329U CN204111753U CN 204111753 U CN204111753 U CN 204111753U CN 201420533329 U CN201420533329 U CN 201420533329U CN 204111753 U CN204111753 U CN 204111753U
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Abstract
The utility model provides a kind of high channel nucleic acid extraction purification devices, comprise many connecting legs and many connecting legs pipe lid, described many connecting legs are stamped many connecting legs pipe lid, described many connecting legs comprise square or circular support plate, described supporting plate is provided with several circular holes, described supporting plate surrounding is provided with brace rod between pipe along circular hole bearing of trend down, single tube is inserted with in described circular hole, between described pipe, brace rod can be fixed and support single tube, pipe papilla acustica basilaris is provided with bottom described single tube, described many connecting legs pipe covers and is provided with the many connecting leg lid nipple corresponding to described many connecting legs position and quantity, described many connecting legs lid nipple is embedded in described many connecting legs mouth of pipe, the beneficial effects of the utility model are: under slightly increasing the condition of traditional core acid extraction cost, the working efficiency of nucleic acid extraction purifying can be significantly improved, reach 3.58 to 9.24 times of traditional core acid extraction working efficiency, when mass detection nucleic acid, a large amount of manpower can be saved, material resources.
Description
[technical field]
The utility model relates to a kind of nucleic acid extraction purification devices, particularly relates to a kind of high channel nucleic acid extraction purification devices.
[background technology]
Molecular Biological Detection technology is the crystallization that modern molecular biology and molecular genetics make great progress, day by day add on deep foundation in the understanding of people to the structure of gene and life quintessence's problem such as the expression of gene and regulation and control to produce, in recent years, the methodological study of molecular Biological Detection technology has made great progress, successively establish restriction enzyme mapping, making nucleic acid molecular hybridization, restriction fragment length polymorphism linkage analysis, Progress of Nucleic Acid Amplification Technologies, and the method such as biochip technology, wherein, the technology such as PCR are widely used in medical diagnosis on disease, such as, application round pcr detects influenza, hepatitis, the multiple invasive organism such as tuberculosis is used for diagnosing corresponding disease,
Above-mentioned various molecular Biological Detection technology all needs detected nucleic acid to extract from different kind organism sample also purifying can subsequent detection, no side, have a strong impact on detection sensitivity and accuracy, traditional nucleic acid extraction purification process cost is low, every 1 to 2 yuan, routine sample, be widely used in each medical and health organization and R&D institution, for this hospital (diformazan hospital), often criticize and detect influenza virus, adenovirus, respiratory syncytial virus, hepatitis B virus, papilloma virus, separate urine Zhi Yuanben, pneumonia Zhi Yuanben, trachoma clothing originally, the pathogenic microorganism nucleic acid such as pathogenic colon bacillus all reach 20 ~ 94 examples, these methods are all single part of single tube (single passage) operations because not having " high-throughput nucleic acid extraction gasifying device ", in the many increment of detection this time, workload very large, spend a large amount of manpower, development in recent years paramagnetic particle method can realize automatization and high-throughput extracts, but its cost is 5 to 10 times of Traditional Method, be not widely used, so utility model high channel nucleic acid extraction purification devices has very high practical value and application prospect.
[utility model content]
The purpose of this utility model is that solving these methods because not having " high-throughput nucleic acid extraction gasifying device " is all single part of single tube (single passage) operation, in the many increment of detection this time, workload very large, spend a large amount of manpower, development in recent years paramagnetic particle method can realize automatization and high-throughput extracts, but its cost is 5 to 10 times of Traditional Method, the deficiency be not widely used and a kind of novel high channel nucleic acid extraction purification devices provided.
The utility model is achieved through the following technical solutions: a kind of high channel nucleic acid extraction purification devices, comprise many connecting legs and many connecting legs pipe lid, described many connecting legs are stamped many connecting legs pipe lid, described many connecting legs comprise square or circular support plate, described supporting plate is provided with several circular holes, described supporting plate surrounding is provided with brace rod between pipe along circular hole bearing of trend down, single tube is inserted with in described circular hole, between described pipe, brace rod can be fixed and support single tube, pipe papilla acustica basilaris is provided with bottom described single tube, described many connecting legs pipe covers and is provided with the many connecting leg lid nipple corresponding to described many connecting legs position and quantity, described many connecting legs lid nipple is embedded in described many connecting legs mouth of pipe.
Further, the length of side of the square set plate of described many connecting legs is: 20 to 60mm, the diameter of the circular support plate of described many connecting legs is: 20 to 60mm, single tube inwall is light face, inner face at the bottom of pipe is hair side, with the nucleic acid precipitation at the bottom of fixed tube, the length of side of described many connecting legs pipe lid is: 20 to 60mm, to adapt to the many connecting legs of different model.
Further, the pipe number of described many connecting legs is 3 to 100 pipes, and be 3 to 100 passages to realize high channel, described many connecting legs lid nipple is: 3 to 100, to adapt to the many connecting legs of different model.
Further, the single tube diameter of described many connecting legs is: 5.0 to 20.0mm, and to adapt to different nucleic acid extraction kit to reagent dosage requirement, the diameter of described many connecting legs lid nipple: 5.0 to 20.0mm, to adapt to the many connecting legs of different model.
Further, the adjacent two pipe tube edge spacing of described many connecting legs are: 0.3 to 3.0mm, prevents from polluting between pipe, the spacing of described many connecting legs lid nipple: 0.3 to 3.0mm, to adapt to the many connecting legs of different model.
Further, the adjacent two pipe die spacing of described many connecting legs are: 8.0 to 18.0mm, to adapt to 8 Dao He 12 road pipettor requirements.
Further, the height of described many connecting legs is: 20.0 to 50.0mm, to adapt to different nucleic acid extraction kit to reagent dosage requirement, the thickness of pipe of described many connecting legs is: 0.5 to 1.2mm, to adapt to different heating and cooling thermal transmission requirement, the height of nipples of described many connecting legs lid: 2.0 to 6.0mm, to adapt to the many connecting legs of different model.
Further, the external diameter of described many connecting legs papilla acustica basilaris is 2.0 to 6.0mm, with the nucleic acid precipitation at the bottom of fixed tube; The internal diameter of described many connecting legs papilla acustica basilaris is 0.5 to 5.0mm, with the nucleic acid precipitation at the bottom of fixed tube; The hole depth of described many connecting legs papilla acustica basilaris is 0.5 to 5.0mm, with the nucleic acid precipitation at the bottom of fixed tube.
Further, the supporting plate of described many connecting legs, from the distance of the mouth of pipe: 2.0 to 30.0mm, is conveniently taken; Brace rod height: 10.0 to 40.0mm between the pipe of described many connecting legs, strengthening physical strength, to tolerate the high centrifugal force function demand of 5000 to 20000g.
Further, the material of described many connecting legs is: polystyrene or polypropylene, can tolerate the high centrifugal force function demand of 5000 to 20000g, and the material of described multitube lid is: polystyrene or silica gel, to adapt to the many connecting legs of different model.
The beneficial effects of the utility model are: under slightly increasing the condition of traditional core acid extraction cost, the working efficiency of nucleic acid extraction purifying can be significantly improved, reach 3.58 to 9.24 times of traditional core acid extraction working efficiency, when mass detection nucleic acid, can a large amount of human and material resources be saved.
[accompanying drawing explanation]
Fig. 1 is the utility model square many connecting legs plan structure schematic diagram;
Fig. 2 is that structural representation looked up by the many connecting legs of the utility model square;
Fig. 3 is the utility model round many connecting legs plan structure schematic diagram;
Fig. 4 is the utility model many connecting legs pipe lid plan structure schematic diagram;
Fig. 5 is that the utility model many connecting legs pipe lid looks up structural representation;
Reference numeral: 1, many connecting legs; 11, supporting plate; 12, brace rod between pipe; 13, single tube; 130, pipe papilla acustica basilaris; 14, circular hole; 2, many connecting legs pipe lid; 21, many connecting legs lid nipple.
[embodiment]
Below in conjunction with the drawings and the specific embodiments, the utility model is described further:
Embodiment 1: with the viral DNA in the utility model and traditional method difference extraction purification 20 parts of serum samples, result of implementation is in table 1.Result shows, when detecting sample and being 20 example, total extraction purification cost increases by 10.0 yuan, shortens working hours 48 minutes, increases work efficiency 3.58 times, and two kinds of method subsequent detection results are consistent.
As Fig. 1, Fig. 2, Fig. 3, Fig. 4, shown in Fig. 5, a kind of high channel nucleic acid extraction purification devices, comprise many connecting legs 1 and many connecting legs pipe lid 2, described many connecting legs 1 are stamped many connecting legs pipe lid 2, described many connecting legs 1 comprise square set plate 11, described supporting plate 11 is provided with several circular holes 14, described supporting plate 11 surrounding is provided with brace rod 12 between pipe along circular hole 14 bearing of trend down, single tube 13 is inserted with in described circular hole 14, between described pipe, brace rod 12 can be fixed and support single tube 13, pipe papilla acustica basilaris 130 is provided with bottom described single tube 13, described many connecting legs pipe lid 2 is provided with the many connecting leg lid nipple 21 corresponding to described many connecting leg 1 positions and quantity, described many connecting legs lid nipple 21 is embedded in described many connecting legs 1 mouth of pipe.
Preferably, the length of side of the square set plate of described many connecting legs 1 is: 20mm, and single tube 13 inwall is light face, and inner face at the bottom of pipe is hair side, and with the nucleic acid precipitation at the bottom of fixed tube, the length of side of described many connecting legs lid 2 is: 20mm, to adapt to the many connecting legs 1 of different model.
Preferably, the pipe number of described many connecting legs 1 is 3 pipes, is 3 passages to realize high channel, and described many connecting legs lid nipple 21 is 3, to adapt to the many connecting legs 1 of different model.
Preferably, single tube 13 diameter of described many connecting legs 1 is 5.0mm, and to adapt to different nucleic acid extraction kit to reagent dosage requirement, the diameter of described many connecting legs lid nipple 21: 5.0mm, to adapt to the many connecting legs 1 of different model.
Preferably, the adjacent two pipe tube edge spacing of described many connecting legs 1 are: 0.3mm, prevents from polluting between pipe, the spacing of described many connecting legs lid nipple 21: 0.3m, to adapt to the many connecting legs of different model.
Preferably, the adjacent two pipe die spacing of described many connecting legs 1 are: 8.0mm, to adapt to 8 Dao He 12 road pipettor requirements.
Preferably, the height of described many connecting legs 1 is: 20.0mm, to adapt to different nucleic acid extraction kit to reagent dosage requirement, the thickness of pipe of described many connecting legs 1 is: 0.5mm, to adapt to different heating and cooling thermal transmission requirement, nipple 21 height of described many connecting legs lid: 2.0mm, to adapt to the many connecting legs of different model.
Preferably, the external diameter of described many connecting legs papilla acustica basilaris 130 is 2.0mm, with the nucleic acid precipitation at the bottom of fixed tube; The internal diameter of described many connecting legs papilla acustica basilaris 130 is 0.5mm, with the nucleic acid precipitation at the bottom of fixed tube; The hole depth of described many connecting legs papilla acustica basilaris 130 is 0.5mm, with the nucleic acid precipitation at the bottom of fixed tube.
Preferably, the supporting plate 11 of described many connecting legs 1, from the distance of the mouth of pipe: 2.0mm, is conveniently taken; Brace rod 12 height: 10.0mm between the pipe of described many connecting legs 1, strengthening physical strength, to tolerate the high centrifugal force function demand of 5000g.
Preferably, the material of described many connecting legs 1 is: polystyrene, can tolerate the high centrifugal force function demand of 5000g, and the material of described multitube lid 2 is: polystyrene, to adapt to the many connecting legs of different model.
Embodiment 2: with the viral DNA in the utility model and traditional method difference extraction purification 100 parts of serum samples, result of implementation is in table 2.Result shows, when detecting sample and being 100 example, total extraction purification cost increases by 40.0 yuan, shortens working hours 254 minutes, increases work efficiency 6.87 times, and two kinds of method subsequent detection results are consistent.
As Fig. 1, Fig. 2, Fig. 3, Fig. 4, shown in Fig. 5, a kind of high channel nucleic acid extraction purification devices, comprise many connecting legs 1 and many connecting legs pipe lid 2, described many connecting legs 1 are stamped many connecting legs pipe lid 2, described many connecting legs 1 comprise square set plate 11, described supporting plate 11 is provided with several circular holes 14, described supporting plate 11 surrounding is provided with brace rod 12 between pipe along circular hole 14 bearing of trend down, single tube 13 is inserted with in described circular hole 14, between described pipe, brace rod 12 can be fixed and support single tube 13, pipe papilla acustica basilaris 130 is provided with bottom described single tube 13, described many connecting legs pipe lid 2 is provided with the many connecting leg lid nipple 21 corresponding to described many connecting leg 1 positions and quantity, described many connecting legs lid nipple 21 is embedded in described many connecting legs 1 mouth of pipe.
Preferably, the diameter of the circular support plate 11 of described many connecting legs 1 is: 50mm, and single tube inwall is light face, and inner face at the bottom of pipe is hair side, and with the nucleic acid precipitation at the bottom of fixed tube, the length of side of described many connecting legs pipe lid 2 is: 50mm, to adapt to the many connecting legs of different model.
Preferably, the pipe number of described many connecting legs 1 is 97 pipes, is 97 passages to realize high channel, and described many connecting legs lid nipple 21 is: 97, to adapt to the many connecting legs of different model.
Preferably, single tube 13 diameter of described many connecting legs 1 is: 15mm, and to adapt to different nucleic acid extraction kit to reagent dosage requirement, the diameter of described many connecting legs lid nipple 21: 15mm, to adapt to the many connecting legs of different model.
Preferably, the adjacent two pipe tube edge spacing of described many connecting legs 1 are:: 18mm, prevents from polluting between pipe, the spacing of described many connecting legs lid nipple: 18mm, to adapt to the many connecting legs of different model.
Preferably, the adjacent two pipe die spacing of described many connecting legs 1 are: 15mm, to adapt to 8 Dao He 12 road pipettor requirements.
Preferably, the height of described many connecting legs 1 is: 40mm, to adapt to different nucleic acid extraction kit to reagent dosage requirement, the thickness of pipe of described many connecting legs 1 is: 1.0mm, to adapt to different heating and cooling thermal transmission requirement, the height of nipples of described many connecting legs lid: 4.5mm, to adapt to the many connecting legs of different model.
Preferably, the external diameter of described many connecting legs papilla acustica basilaris 21 is 4.5mm, with the nucleic acid precipitation at the bottom of fixed tube; The internal diameter of described many connecting legs papilla acustica basilaris 21 is 4.5mm, with the nucleic acid precipitation at the bottom of fixed tube; The hole depth of described many connecting legs papilla acustica basilaris 21 is 4.5mm, with the nucleic acid precipitation at the bottom of fixed tube.
Preferably, the supporting plate 11 of described many connecting legs 1, from the distance of the mouth of pipe: 27.0mm, is conveniently taken; Brace rod 12 height: 35.0mm between the pipe of described many connecting legs 1, strengthening physical strength, to tolerate the high centrifugal force function demand of 18000g.
Preferably, the material of described many connecting legs 1 is: polypropylene, can tolerate the high centrifugal force function demand of 18000g, and the material of described multitube lid 2 is: silica gel, to adapt to the many connecting legs of different model.
Embodiment 3: with the mycoplasma DNA in the utility model and traditional method difference extraction purification 100 parts of throat swab samples, result of implementation is in table 3.Result shows, when detecting sample and being 100 example, total extraction purification cost increases by 40.0 yuan, shortens working hours 326 minutes, increases work efficiency 9.24 times, and two kinds of method subsequent detection results are consistent.
As Fig. 1, Fig. 2, Fig. 3, Fig. 4, shown in Fig. 5, a kind of high channel nucleic acid extraction purification devices, comprise many connecting legs 1 and many connecting legs pipe lid 2, described many connecting legs 1 are stamped many connecting legs pipe lid 2, described many connecting legs 1 comprise square set plate 11, described supporting plate 11 is provided with several circular holes 14, described supporting plate 11 surrounding is provided with brace rod 12 between pipe along circular hole 14 bearing of trend down, single tube 13 is inserted with in described circular hole 14, between described pipe, brace rod 12 can be fixed and support single tube 13, pipe papilla acustica basilaris 130 is provided with bottom described single tube 13, described many connecting legs pipe lid 2 is provided with the many connecting leg lid nipple 21 corresponding to described many connecting leg 1 positions and quantity, described many connecting legs lid nipple 21 is embedded in described many connecting legs 1 mouth of pipe.
Preferably, the length of side of the square set plate 11 of described many connecting legs 1 is: 60mm, and single tube inwall is light face, and inner face at the bottom of pipe is hair side, and with the nucleic acid precipitation at the bottom of fixed tube, the length of side of described many connecting legs pipe lid 2 is: 60mm, to adapt to the many connecting legs of different model.
Preferably, the pipe number of described many connecting legs 1 is 100 pipes, is 100 passages to realize high channel, and described many connecting legs lid nipple 21 is: 100, to adapt to the many connecting legs of different model.
Preferably, single tube 13 diameter of described many connecting legs 1 is: 20.0mm, and to adapt to different nucleic acid extraction kit to reagent dosage requirement, the diameter of described many connecting legs lid nipple 21: 20.0mm, to adapt to the many connecting legs of different model.
Preferably, the adjacent two pipe tube edge spacing of described many connecting legs 1 are: 3.0mm, prevents from polluting between pipe, the spacing of described many connecting legs lid nipple 21: 3.0mm, to adapt to the many connecting legs of different model.
Preferably, the adjacent two pipe die spacing of described many connecting legs 1 are: 18.0mm, to adapt to 8 Dao He 12 road pipettor requirements.
Preferably, the height of described many connecting legs 1 is: 50.0mm, to adapt to different nucleic acid extraction kit to reagent dosage requirement, the thickness of pipe of described many connecting legs 1 is: 1.2mm, to adapt to different heating and cooling thermal transmission requirement, nipple 21 height of described many connecting legs lid: 6.0mm, to adapt to the many connecting legs of different model.
Preferably, the external diameter of described many connecting legs papilla acustica basilaris 21 is 6.0mm, with the nucleic acid precipitation at the bottom of fixed tube; The internal diameter of described many connecting legs papilla acustica basilaris 21 is 5.0mm, with the nucleic acid precipitation at the bottom of fixed tube; The hole depth of described many connecting legs papilla acustica basilaris 21 is 5.0mm, with the nucleic acid precipitation at the bottom of fixed tube.
Preferably, the supporting plate 11 of described many connecting legs 1, from the distance of the mouth of pipe: 30.0mm, is conveniently taken; Brace rod 12 height: 40.0mm between the pipe of described many connecting legs 1, strengthening physical strength, to tolerate the high centrifugal force function demand of 20000g.
Preferably, the material of described many connecting legs 1 is: polystyrene, can tolerate the high centrifugal force function demand of 20000g, and the material of described multitube pipe lid 2 is: polystyrene, to adapt to the many connecting legs of different model.
Table 1: two kinds of method extraction purification 20 routine serum sample viral DNA efficiency comparison tables
Table 2: two kinds of method extraction purification 100 routine serum sample viral DNA efficiency comparison tables
Table 3: two kinds of method extraction purification 100 routine throat swab sample mycoplasma DNA efficiency comparison tables
The announcement of book and instruction according to the above description, the utility model those skilled in the art can also carry out suitable change and amendment to above-mentioned embodiment.Therefore, the utility model is not limited to embodiment disclosed and described above, also should fall in the protection domain of claim of the present utility model modifications and changes more of the present utility model.In addition, although employ some specific terms in this specification sheets, these terms just for convenience of description, do not form any restriction to the utility model.
Claims (10)
1. a high channel nucleic acid extraction purification devices, it is characterized in that: comprise many connecting legs and many connecting legs pipe lid, described many connecting legs are stamped many connecting legs pipe lid, described many connecting legs comprise square or circular support plate, described supporting plate is provided with several circular holes, described supporting plate surrounding is provided with brace rod between pipe along circular hole bearing of trend down, single tube is inserted with in described circular hole, between described pipe, brace rod can be fixed and support single tube, pipe papilla acustica basilaris is provided with bottom described single tube, described many connecting legs pipe covers and is provided with the many connecting leg lid nipple corresponding to described many connecting legs position and quantity, described many connecting legs lid nipple is embedded in described many connecting legs mouth of pipe.
2. high channel nucleic acid extraction purification devices according to claim 1, it is characterized in that: the length of side of the square set plate of described many connecting legs is: 20 to 60mm, the diameter of the circular support plate of described many connecting legs is: 20 to 60mm, single tube inwall is light face, inner face at the bottom of pipe is hair side, with the nucleic acid precipitation at the bottom of fixed tube, the length of side of described many connecting legs pipe lid is: 20 to 60mm, to adapt to the many connecting legs of different model.
3. high channel nucleic acid extraction purification devices according to claim 1, is characterized in that: the pipe number of described many connecting legs is 3 to 100 pipes, and be 3 to 100 passages to realize high channel, described many connecting legs lid nipple is: 3 to 100, to adapt to the many connecting legs of different model.
4. high channel nucleic acid extraction purification devices according to claim 1, it is characterized in that: the single tube diameter of described many connecting legs is: 5.0 to 20.0mm, to adapt to different nucleic acid extraction kit to reagent dosage requirement, the diameter of described many connecting legs lid nipple: 5.0 to 20.0mm, to adapt to the many connecting legs of different model.
5. high channel nucleic acid extraction purification devices according to claim 1, it is characterized in that: the adjacent two pipe tube edge spacing of described many connecting legs are: 0.3 to 3.0mm, prevent from polluting between pipe, the spacing of described many connecting legs lid nipple: 0.3 to 3.0mm, to adapt to the many connecting legs of different model.
6. high channel nucleic acid extraction purification devices according to claim 1, is characterized in that: the adjacent two pipe die spacing of described many connecting legs are: 8.0 to 18.0mm, to adapt to 8 Dao He 12 road pipettor requirements.
7. high channel nucleic acid extraction purification devices according to claim 1, it is characterized in that: the height of described many connecting legs is: 20.0 to 50.0mm, to adapt to different nucleic acid extraction kit to reagent dosage requirement, the thickness of pipe of described many connecting legs is: 0.5 to 1.2mm, to adapt to different heating and cooling thermal transmission requirement, the height of nipples of described many connecting legs lid: 2.0 to 6.0mm, to adapt to the many connecting legs of different model.
8. high channel nucleic acid extraction purification devices according to claim 1, is characterized in that: the external diameter of described many connecting legs papilla acustica basilaris is 2.0 to 6.0mm, with the nucleic acid precipitation at the bottom of fixed tube; The internal diameter of described many connecting legs papilla acustica basilaris is 0.5 to 5.0mm, with the nucleic acid precipitation at the bottom of fixed tube; The hole depth of described many connecting legs papilla acustica basilaris is 0.5 to 5.0mm, with the nucleic acid precipitation at the bottom of fixed tube.
9. high channel nucleic acid extraction purification devices according to claim 1, is characterized in that: the supporting plate of described many connecting legs is from the distance of the mouth of pipe: 2.0 to 30.0 mm, conveniently takes; Brace rod height: 10.0 to 40.0mm between the pipe of described many connecting legs, strengthening physical strength, to tolerate the high centrifugal force function demand of 5000 to 20000g.
10. high channel nucleic acid extraction purification devices according to claim 1, it is characterized in that: the material of described many connecting legs is: polystyrene or polypropylene, the high centrifugal force function demand of 5000 to 20000g can be tolerated, the material of described multitube lid is: polystyrene or silica gel, to adapt to the many connecting legs of different model.
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| CN201420533329.XU CN204111753U (en) | 2014-09-17 | 2014-09-17 | A kind of high channel nucleic acid extraction purification devices |
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| CN201420533329.XU CN204111753U (en) | 2014-09-17 | 2014-09-17 | A kind of high channel nucleic acid extraction purification devices |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232476A (en) * | 2014-09-17 | 2014-12-24 | 深圳市宝安区沙井人民医院 | High-channel nucleic acid extraction and purification device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232476A (en) * | 2014-09-17 | 2014-12-24 | 深圳市宝安区沙井人民医院 | High-channel nucleic acid extraction and purification device |
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