CN204086146U - A kind of light channel structure of time-resolved fluorescence immunoassay instrument - Google Patents

A kind of light channel structure of time-resolved fluorescence immunoassay instrument Download PDF

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Publication number
CN204086146U
CN204086146U CN201420612425.3U CN201420612425U CN204086146U CN 204086146 U CN204086146 U CN 204086146U CN 201420612425 U CN201420612425 U CN 201420612425U CN 204086146 U CN204086146 U CN 204086146U
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China
Prior art keywords
light
optical filter
time
immunoassay instrument
collecting lens
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CN201420612425.3U
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Chinese (zh)
Inventor
李明
周诗寒
区仲荣
谢剑锋
洪俊安
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Guangzhou Da Rui Biotechnology Ltd
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Guangzhou Da Rui Biotechnology Ltd
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Abstract

The utility model discloses a kind of light channel structure of time-resolved fluorescence immunoassay instrument, it is characterized in that, comprise excitation light path and receiving light path, described excitation light path comprises light source, the first light-collecting lens, the first optical filter, second condenser lens sheet and color separation light splitting piece that horizontal direction sets gradually, color separation light splitting piece is arranged from the horizontal by miter angle, be provided with the second optical filter below color separation light splitting piece, below the second optical filter, place reagent sample; Described receiving light path comprises the first fluorescence light-collecting lens, the 3rd optical filter and the second fluorescence light-collecting lens that vertical direction are set in turn in below reagent sample.The light channel structure of the utility model time-resolved fluorescence immunoassay instrument is simple and take up room little.

Description

A kind of light channel structure of time-resolved fluorescence immunoassay instrument
Technical field
The utility model relates to time resolved fluoro-immunoassay field, more specifically, relates to a kind of light channel structure of time-resolved fluorescence immunoassay instrument.
Background technology
Time-resolved fluorescence immunoassay instrument is a kind of instrument utilizing rare earth label to carry out ultramicro-analysis.Its ultimate principle is that antibody rare earth element is marked generate a reagent, and under the irradiation of specific light source, reagent inspires fluorescence, through delay after a while, background interference fluorescence is eliminated, is the required fluorescence sent by label, optical analysis is carried out to this fluorescence, can diagnosis be made.
Time-resolved fluorescence immunoassay instrument light channel structure directly has influence on the efficiency of detection, and in order to reach higher detection efficiency, the more complicated that light channel structure generally does, volume is also larger.
Utility model content
The utility model, for overcoming at least one defect (deficiency) described in above-mentioned prior art, provides a kind of light channel structure of simple, time-resolved fluorescence immunoassay instrument that volume is little.
For solving the problems of the technologies described above, the technical solution of the utility model is as follows:
A kind of light channel structure of time-resolved fluorescence immunoassay instrument, comprise excitation light path and receiving light path, described excitation light path comprises light source, the first light-collecting lens, the first optical filter, second condenser lens sheet and color separation light splitting piece that horizontal direction sets gradually, color separation light splitting piece is arranged from the horizontal by miter angle, be provided with the second optical filter below color separation light splitting piece, below the second optical filter, place reagent sample;
Described receiving light path comprises the first fluorescence light-collecting lens, the 3rd optical filter and the second fluorescence light-collecting lens that vertical direction are set in turn in below reagent sample.
In the preferred scheme of one, in the horizontal direction, LED condenser and the first photo-detector is also disposed with after the color separation light splitting piece of described excitation light path.
In the preferred scheme of one, between the color separation light splitting piece of described excitation light path and the second optical filter, be provided with the first logical light valve.
In the preferred scheme of one, below the second fluorescence light-collecting lens of described receiving light path, be provided with the second photo-detector.
In the preferred scheme of one, between the second fluorescence light-collecting lens of described receiving light path and the second photo-detector, be provided with the second logical light valve.
In the preferred scheme of one, described first optical filter and the second optical filter are 340nm optical filter.
In the preferred scheme of one, described 3rd optical filter is 613nm optical filter.
In the preferred scheme of one, between the first fluorescence light-collecting lens of described receiving light path and reagent sample, be provided with cover glass.
Compared with prior art, the beneficial effect of technical solutions of the utility model is: the light channel structure of the utility model time-resolved fluorescence immunoassay instrument, the light level that light source sends is through the first light-collecting lens, first optical filter and second condenser lens sheet, then through color separation light splitting piece, a part of light is reflected, this part light is irradiated on reagent sample through the second optical filter straight down and produces fluorescence, fluorescence is straight down successively through the first fluorescence light-collecting lens, 3rd optical filter and the second fluorescence light-collecting lens, light channel structure is simple and take up room little, and optical routing level transfers to vertically by the reflection of color separation light splitting piece, the overall volume of light channel structure reduces further, another light converges to the first photo-detector through LED condenser after the transmission of color separation light splitting piece, first photo-detector detects the intensity of this part light also to the control circuit feedback signal of light source, control circuit adjusts voltage or the electric current of supply light source automatically by feedback signal, to ensure that the intensity of the exciting light needed for detection is in the scope preset, excitation light path is through the optical filtering of 2 tablet filters, and excitation wavelength is more single, disturb less, result is more accurate.
Accompanying drawing explanation
Fig. 1 is the light channel structure figure of the utility model time-resolved fluorescence immunoassay instrument.
1, light source; 2, the first light-collecting lens; 3, the first optical filter; 4, second condenser lens sheet; 5, color separation light splitting piece; 6, LED condenser; 7, the first photo-detector; 8, the first logical light valve; 9, the second optical filter; 10, reagent sample; 11, cover glass; 12, the first fluorescence light-collecting lens; 13, the 3rd optical filter; 14, the second fluorescence light-collecting lens; 15, the second logical light valve; 16, the second photo-detector.
Embodiment
Accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent;
In order to better the present embodiment is described, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product;
To those skilled in the art, in accompanying drawing, some known features and explanation thereof may be omitted is understandable.
Below in conjunction with drawings and Examples, the technical solution of the utility model is described further.
Embodiment 1
As shown in Figure 1, a kind of light channel structure of time-resolved fluorescence immunoassay instrument, comprise excitation light path and receiving light path, described excitation light path comprises light source 1, first light-collecting lens 2, first optical filter 3, second condenser lens sheet 4, color separation light splitting piece 5, LED condenser 6 and the first photo-detector 7 that horizontal direction sets gradually, color separation light splitting piece 5 is arranged from the horizontal by miter angle, be disposed with below color separation light splitting piece 5 below first logical light valve 8 and the second optical filter 9, second optical filter 9 and place reagent sample 10;
Described receiving light path comprises cover glass 11, the first fluorescence light-collecting lens 12, the logical light valve 15 of the 3rd optical filter 13, second fluorescence light-collecting lens 14, second and the second photo-detector 16 that vertical direction are set in turn in below reagent sample 10.
In concrete real-time process, described first optical filter 3 and the second optical filter 9 are 340nm optical filter, and described 3rd optical filter 13 is 613nm optical filter.
The principle of the light channel structure of the utility model time-resolved fluorescence immunoassay instrument is: the light level that light source 1 sends is through the first light-collecting lens 2, first optical filter 3 and second condenser lens sheet 4, part light is through the reflection of color separation light splitting piece 5, this part light is irradiated on reagent sample 10 through the second optical filter 9 straight down and produces fluorescence, fluorescence is straight down successively through cover glass 11, first fluorescence light-collecting lens 12, 3rd optical filter 13 and the second fluorescence light-collecting lens 14, finally received by the second photo-detector 16 and measure, the concentration of reagent sample 10 can be judged according to the intensity of fluorescence, another light converges to the first photo-detector 7 through LED condenser 6 after color separation light splitting piece 5 transmission, first photo-detector 7 detects the intensity of this part light and to the control circuit feedback signal of light source 1, control circuit adjusts voltage or the electric current of supply light source 1 automatically by feedback signal.
The light channel structure of the utility model time-resolved fluorescence immunoassay instrument is simple and take up room little, and optical routing level transfers to vertically by the reflection of color separation light splitting piece 5, and the overall volume of light channel structure reduces further.
The feedback of the control circuit of the utility model first photo-detector 7 pairs of light sources 1, can ensure that the intensity of the exciting light needed for detecting is in the scope preset.
The utility model excitation light path is through the optical filtering of 2 tablet filters, and excitation wavelength is more single, disturb less, result is more accurate.
Of the present utility model first logical light valve 8 and the second logical propagation bore of light valve 15 to light limit, thus control logical light quantity.
The corresponding same or analogous parts of same or analogous label;
The term describing position relationship in accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent;
Obviously, above-described embodiment of the present utility model is only for the utility model example is clearly described, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.All do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., within the protection domain that all should be included in the utility model claim.

Claims (8)

1. the light channel structure of a time-resolved fluorescence immunoassay instrument, it is characterized in that, comprise excitation light path and receiving light path, described excitation light path comprises light source, the first light-collecting lens, the first optical filter, second condenser lens sheet and color separation light splitting piece that horizontal direction sets gradually, color separation light splitting piece is arranged from the horizontal by miter angle, be provided with the second optical filter below color separation light splitting piece, below the second optical filter, place reagent sample;
Described receiving light path comprises the first fluorescence light-collecting lens, the 3rd optical filter and the second fluorescence light-collecting lens that vertical direction are set in turn in below reagent sample.
2. the light channel structure of time-resolved fluorescence immunoassay instrument according to claim 1, is characterized in that, in the horizontal direction, is also disposed with LED condenser and the first photo-detector after the color separation light splitting piece of described excitation light path.
3. the light channel structure of time-resolved fluorescence immunoassay instrument according to claim 1, is characterized in that, is provided with the first logical light valve between the color separation light splitting piece of described excitation light path and the second optical filter.
4. the light channel structure of time-resolved fluorescence immunoassay instrument according to claim 1, is characterized in that, is provided with the second photo-detector below the second fluorescence light-collecting lens of described receiving light path.
5. the light channel structure of time-resolved fluorescence immunoassay instrument according to claim 4, is characterized in that, is provided with the second logical light valve between the second fluorescence light-collecting lens of described receiving light path and the second photo-detector.
6. the light channel structure of time-resolved fluorescence immunoassay instrument according to claim 1, is characterized in that, described first optical filter and the second optical filter are 340nm optical filter.
7. the light channel structure of time-resolved fluorescence immunoassay instrument according to claim 1, is characterized in that, described 3rd optical filter is 613nm optical filter.
8. the light channel structure of the time-resolved fluorescence immunoassay instrument according to any one of claim 1-7, is characterized in that, is provided with cover glass between the first fluorescence light-collecting lens of described receiving light path and reagent sample.
CN201420612425.3U 2014-10-22 2014-10-22 A kind of light channel structure of time-resolved fluorescence immunoassay instrument Active CN204086146U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106706574A (en) * 2015-11-12 2017-05-24 中国科学院大连化学物理研究所 Weak fluorescence detection device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106706574A (en) * 2015-11-12 2017-05-24 中国科学院大连化学物理研究所 Weak fluorescence detection device
CN106706574B (en) * 2015-11-12 2019-03-19 中国科学院大连化学物理研究所 A kind of detection device of hypofluorescence

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