CN204086118U - A kind of permanent liquid microsphere sample room - Google Patents
A kind of permanent liquid microsphere sample room Download PDFInfo
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- CN204086118U CN204086118U CN201420591155.2U CN201420591155U CN204086118U CN 204086118 U CN204086118 U CN 204086118U CN 201420591155 U CN201420591155 U CN 201420591155U CN 204086118 U CN204086118 U CN 204086118U
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- cover glass
- microslide
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- slide
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Abstract
The utility model discloses a kind of permanent liquid microsphere sample room, comprise cover glass (1), slide interlayer (2) and microslide (3); Be processed into rectangle hole in the centre of slide interlayer, its length, can for sample introduction and the port of giving vent to anger to reserve at cavity two ends slightly larger than cover glass length; Slide interlayer has certain thickness, is clipped in the strip cavity being formed between microslide and cover glass and can hold sample solution; Utilize the hot melt of slide interlayer or himself viscosity that cover glass and microslide are bonded to sample chamber.The utility model can be reused, and can long-time storage, and it has good chemical stability and high/low temperature and feels bad power.This sample chamber selection and physical dimension design for conventional microscopy observation, can be used for the experiment of most optical microphotograph, can observe for a long time, do not have the user of correlation experience also can directly use to fluid sample.The utility model is not limited only to liquid microsphere sample, is also applicable to all liquid samples of normal conventional.
Description
Technical field
The utility model belongs to micro-container technical field, and be specifically related to a kind of permanent liquid microsphere sample room, this liquid microsphere sample room is the device of a kind of long-term storage and use repeatly.
Background technology
Along with microcosmic the reach of science, people are more deep to the research of micro-nano-scale particulate, the microexamination of such as particulate, the interactional dynamic process of particulate, and the measurement etc. that particulate is stressed, and these researchs all need the vessel depositing microsphere suspension liquid.
When the vessel of micro-nano microparticle suspending liquid are deposited in making, at every turn common way tests the new sample chamber of making one, and so, not only the Preparatory work of experiment time is long, also wastes experiment material.In addition, because sample can not be reused, which also restricts research range, the constantly meticulous and deep demand of scientific research can not be met.
Therefore, the long-term food preservation test sample of energy, and the permanent liquid microsphere sample room that can repeatedly use just seems very important.But, in the process of long-term storage, there is sealing difficulty in permanent liquid microsphere sample room, easy endophytic bacteria, the problem such as the easy aggregate and precipitate of microballoon in solution, the utility model is intended to solve the problem, and provides a kind of practicable permanent liquid microsphere sample room production program, and it is expanded, make it also be applicable to all liquid samples of normal conventional.
Utility model content
The utility model object is, provides a kind of permanent liquid microsphere sample room, and it is specially can longer-term storage depositing and finder the sample of Reusability, and can be widely used in every microscopic observation and experimental study.
Based on above-mentioned purpose, the utility model proposes a kind of permanent liquid microsphere sample room, comprise cover glass, slide interlayer and microslide.Processing rectangle hole in the middle of slide interlayer, its length, can sample introduction and the port of giving vent to anger to reserve at cavity two ends slightly larger than cover glass length; Slide interlayer has certain thickness, is clipped in the strip cavity being formed between microslide and cover glass and can hold sample solution; Utilize the hot melt of slide interlayer or himself viscosity that cover glass and microslide are bonded to sample chamber container.
Further, in sample chamber, sample solution comprises microsphere suspension liquid, casein, sodium azide and other organic solvents etc.The concentration of microsphere suspension liquid is determined as required; Trace casein can prevent the microballoon in sample from adhering on slide or sidewall, and can prevent microballoon agglomerate; Appropriate sodium azide can anticorrosion sterilizing; Other organic solvents are generally the material that user studies, also the Conventional solvents such as water.The content of casein and sodium azide is extremely low, and physical property and the solvent of solution are close.
Further, after injecting microsphere sample in sample cavity, with viscous grease (as vaseline, Hmp grease etc.) by strip cavity closed at both ends, liquid-to-air is completely cut off, avoid evaporating, and then the surrounding of sample chamber is sealed up with 703 glue, sealing function is provided, also plays the effect of reinforcing sample chamber simultaneously.
Further, sample chamber ultraviolet light sterilization after assembling, ensure that microsphere sample is without bacterium, long-term storage is not perishable.
The utility model has the advantage of:
(1), liquid microsphere sample pollution-free, do not assemble, can long-time storage repeatedly using;
(2), there is good chemical stability and high/low temperature and feel bad power;
(3), sample chamber selection and physical dimension design for microscopic observation, can be used for, in most observation by light microscope or the experiment of light tweezer, not having the user of correlation experience also can directly use;
(4), the permanent liquid microsphere sample room of the present invention is not limited only to liquid microsphere sample, is also applicable to conventional all liquid samples.
Accompanying drawing explanation
Fig. 1 is permanent liquid microsphere sample room structure of container schematic diagram;
Fig. 2 is the physical dimension of each parts of sample chamber container, unit mm:
Fig. 2 (a) is cover glass 1. diagram, is of a size of 22mm × 22mm × 0.17mm;
Fig. 2 (b) is slide interlayer 2. diagram, is of a size of 28mm × 25mm; Endoporus is 24mm × 6mm; Its thickness changes (about 0.16mm) to some extent according to the difference of selection;
Fig. 2 (c) is microslide 3. diagram, is of a size of 75mm × 25mm × 1mm;
Fig. 3 is that sample chamber finished product was placed in refrigerator after 8 months, uses dual access test to catch the experiment sectional drawing of suspension microballoon.
Embodiment
The utility model proposes a kind of permanent liquid microsphere sample room, it is can long-time storage the sample chamber of depositing liquid microsphere sample that can repeatedly use, this sample chamber selection and physical dimension design for microscopic observation, can be used in most observation by light microscope or the experiment of light tweezer.
The cover glass that sample chamber is clean and microslide make, and accompany slide interlayer, then utilize the hot melt of slide interlayer or intrinsic viscosity that three is connected, be assembled into " sample chamber container " between cover glass and microslide.Need processing rectangle hole in the centre of slide interlayer, glass slide clamp thickness about hundred microns, therefore can form the strip cavity holding sample solution between microslide and cover glass; Hole lengths is greater than cover glass length, so that the port forming sample introduction at cavity two ends and give vent to anger.
In sample chamber, sample solution comprises microsphere suspension liquid, casein, sodium azide and other organic solvents etc.The concentration of microsphere suspension liquid is determined as required; Trace casein can prevent the microballoon in sample from adhering on slide or sidewall, and can prevent microballoon agglomerate; Appropriate sodium azide can anticorrosion sterilizing; Other organic solvents are generally the material that user studies, also the Conventional solvents such as water.The content of casein and sodium azide is extremely low, and physical property and the solvent of solution are close.
After injecting microsphere sample in sample cavity, with viscous grease (as vaseline, Hmp grease etc.) by strip cavity closed at both ends, liquid-to-air is completely cut off, avoid evaporating, and then the surrounding of sample chamber is sealed up with 703 glue, also play the effect of reinforcing sample chamber while sealing function is provided.
After assembling, sample chamber container ultraviolet light sterilization, makes microsphere sample without bacterium, can long-term storage not perishable.
The permanent liquid microsphere sample room that the utility model makes, technical indicator as shown in table 1 should be expired:
Table 1
| Project name | Parameter | Verification |
| Sample chamber inwall amount of impurities | Be greater than 30nm particle ~ 0 | Differential interference microscope is observed |
| Reusable number of times | More than 50 times | Conservative estimation |
| Effective storage life | More than 8 months | Experimental verification |
| Particle size can be used | 0.1～20μm | Experimental verification |
| Particulate material | Inorganic material (as polystyrene or silicon dioxide) | Merchant product |
| Sample chamber volume | About 22 μ l | Actual measurement |
| Microballoon concentration | Unrestricted, also can store not containing the ordinary liquid sample of microballoon | Experimental verification |
Instantiation 1 is as follows:
Make the permanent liquid microsphere sample room with good chemical stability.
1. material requested
Microslide (sailing boat board, 75mm × 25mm × 2mm), cover glass (Corning Cover Glass Cat.No.2865-22,22mm × 22mm × 0.17mm), sealed membrane (Parafilm, thickness 0.16mm), Heat desk instrument, vaseline, 703 glue, sterilizing deionized water, saturated (Casein) solution (0.8-1.2%), sodium azide (NaN
3) solution (2%), polyoxyethylene poly-oxygen propylene aether block copolymer solution (Sigma, P5556), 1um diameter polystyrene microsphere suspending liquid (Thermo scientific Catalog#4009A);
2. sample chamber container assembling
Microslide and cover glass are used following cleaning process.
Step one, microslide clean:
1) use liquid detergent to clean, rinse with clear water afterwards, drain;
2) deionized water ultrasonic vibration 5min is used;
3) rinsed with deionized water is used;
4) ultrasonic vibration 5min in alcohol;
5) dry.
Step 2, cover glass clean:
1) use liquid detergent to clean, rinse with clear water afterwards, drain;
2) the ultrasonic 5min of potassium hydroxide solution of 5mol/L is used;
3) rinsed with deionized water is used;
4) deionized water ultrasonic vibration 5min is used;
5) rinsed with deionized water is used;
6) ultrasonic vibration 5min in alcohol;
7) dry.
Step 3, hot melt assembling sample chamber container
Sealed membrane is cut according to Fig. 2-B size, then sealed membrane and cleaned slide are assembled into sample chamber container according to Fig. 1, re-use Heat desk instrument and microslide is heated to 75 DEG C, cover glass and microslide can be sticked together by sealed membrane, and form the cavity of about 22 μ l, two ports are retained, so that application of sample and exhaust at elongated cavity two ends.
3. prepare microspheres solution
Sample solution uses the deionized water of sterilizing as solvent.Contain in final solution, 1% polyoxyethylene poly-oxygen propylene aether segmented copolymer, 0.01%m/m casein, 0.02%m/m sodium azide and the dilution microballoon of 1000 ~ 10000 times.
4. filling and seal
Appropriate solution is injected in the sample chamber container assembled, use vaseline to close two-port, slightly exert pressure with emptying air, finally use 703 glue by the gap closing of sample chamber surrounding.After it dries, put into refrigerator store.
5. store and use
Permanent liquid microsphere sample room is placed in seal box (as crisper), is stored in the low temperature environment (refrigerator cold-storage) of 4 DEG C.Through test, this sample chamber at least can store 8 months, as shown in Figure 3.
6. relative merits
Advantage: because the chemical stability of sealed membrane and vaseline is better, therefore can be used for the sample (as the block copolymer solution in instantiation 1 or not containing the organic solvent class I liquid I of microballoon) holding organic solvent based.
Shortcoming: due to sealed membrane softening temperature and vaseline fusing point all lower, the sample chamber therefore in instantiation 1 is mainly used in the situation that test temperature is no more than 40 DEG C.
Instantiation 2 is as follows:
Make the permanent liquid microsphere sample room that temperature applicable range is wider.
1. material requested
Microslide (sailing boat board, 75mm × 25mm × 2mm), cover glass (sailing boat, 24mm × 24mm × 0.17mm), double faced adhesive tape (thickness 0.15mm), Hmp grease (Mobil Duracell), 703 glue, sterilizing deionized water, saturated (Casein) solution (0.8-1.2%), sodium azide (NaN
3) solution (2%), 1um diameter polystyrene microsphere suspending liquid (Thermo scientific Catalog#4009A);
2. sample chamber container assembling
Microslide and cover glass are used following cleaning process.
Step one, microslide clean:
1) use liquid detergent to clean, rinse with clear water afterwards, drain;
2) deionized water ultrasonic vibration 5min is used;
3) rinsed with deionized water is used;
4) ultrasonic vibration 5min in alcohol;
5) dry.
Step 2, cover glass clean:
1) use liquid detergent to clean, rinse with clear water afterwards, drain;
2) the ultrasonic 5min of potassium hydroxide solution of 5mol/L is used;
3) rinsed with deionized water is used;
4) deionized water ultrasonic vibration 5min is used;
5) rinsed with deionized water is used;
6) ultrasonic vibration 5min in alcohol,
7) dry.
Step 3, assembling sample chamber container
Double faced adhesive tape is cut according to Fig. 2-B size, then double faced adhesive tape and cleaned slide is assembled into sample chamber container according to Fig. 1.Cover glass and microslide can be sticked together by double faced adhesive tape, and form the cavity of about 20 μ l, retain two ports, so that application of sample and exhaust at elongated cavity two ends.
3. prepare microspheres solution
Sample solution uses the deionized water of sterilizing as solvent.Contain in final solution, 0.01%m/m casein, 0.02%m/m sodium azide and the dilution microballoon of 1000 ~ 10000 times.
4. filling and seal
Appropriate solution is injected in the sample chamber container assembled, use Hmp grease to close two-port, slightly exert pressure with emptying air, finally use 703 glue by the gap closing of sample chamber surrounding.After it dries, put into refrigerator store.
5. store and use
Permanent liquid microsphere sample room is placed in seal box (as crisper), is stored in the low temperature environment (refrigerator cold-storage) of 4 DEG C.Through test, this sample chamber at least can store 8 months.
6. relative merits
Comparatively instantiation 1, in instantiation 2, the temperature applicable range of sample chamber is wider, can work at lower than the temperature of boiling point of liquid.But due to the restriction of double faced adhesive tape self property, it cannot be used for holding organic solvent based sample.
Instantiation 3 is as follows:
The utility model sample chamber is compared with common sample room
Common sample room generally uses the surface tension of sample liquids cover glass and microslide to be sticked together, and then uses adhesive tape to fix.Find through contrast experiment, this patent sample chamber and common sample room are distinguished as follows.
The utility model does not elaborate the known technology that part belongs to those skilled in the art.
The above; be only the embodiment in the utility model; but protection domain of the present utility model is not limited thereto; any people being familiar with this technology is in the technical scope disclosed by the utility model; the conversion or replacement expected can be understood; all should be encompassed in and of the present utility modelly comprise within scope, therefore, protection domain of the present utility model should be as the criterion with the protection domain of claims.
Claims (4)
1. a permanent liquid microsphere sample room, is characterized in that: comprise cover glass (1), slide interlayer (2) and microslide (3); Processing rectangle hole in the middle of slide interlayer (2), its length, can for sample introduction and the port of giving vent to anger to reserve at cavity two ends slightly larger than cover glass (1) length; Slide interlayer has certain thickness, is clipped in the strip cavity being formed between microslide (3) and cover glass (1) and can hold sample solution; Utilize the hot melt of slide interlayer or himself viscosity that cover glass (1) and microslide (3) are bonded to sample chamber container.
2. the permanent liquid microsphere sample room of one according to claim 1, is characterized in that: cover glass (1) is square or rectangular, and its width is not more than microslide width.
3. the permanent liquid microsphere sample room of one according to claim 1, is characterized in that: sealed membrane chi (2) is rectangle, and length and width are not more than microslide length and width, and its center is scratched strip square hole, and square hole length should slightly larger than cover glass length.
4. the permanent liquid microsphere sample room of one according to claim 1, is characterized in that: microslide (3) is rectangle, the bulk restriction of demand fulfillment microscope stage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420591155.2U CN204086118U (en) | 2014-10-13 | 2014-10-13 | A kind of permanent liquid microsphere sample room |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420591155.2U CN204086118U (en) | 2014-10-13 | 2014-10-13 | A kind of permanent liquid microsphere sample room |
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| Publication Number | Publication Date |
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| CN204086118U true CN204086118U (en) | 2015-01-07 |
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| CN201420591155.2U Expired - Fee Related CN204086118U (en) | 2014-10-13 | 2014-10-13 | A kind of permanent liquid microsphere sample room |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104266973A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Permanent liquid-state microsphere sample chamber |
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2014
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104266973A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Permanent liquid-state microsphere sample chamber |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150107 Termination date: 20201013 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |