CN203561572U - Fluid system of flow cytometry - Google Patents

Fluid system of flow cytometry Download PDF

Info

Publication number
CN203561572U
CN203561572U CN201320602437.3U CN201320602437U CN203561572U CN 203561572 U CN203561572 U CN 203561572U CN 201320602437 U CN201320602437 U CN 201320602437U CN 203561572 U CN203561572 U CN 203561572U
Authority
CN
China
Prior art keywords
valve
drive unit
way pipe
hemolytic agent
syringe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201320602437.3U
Other languages
Chinese (zh)
Inventor
崔立青
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201320602437.3U priority Critical patent/CN203561572U/en
Application granted granted Critical
Publication of CN203561572U publication Critical patent/CN203561572U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Landscapes

  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The utility model relates to a fluid system of a flow cytometry. The fluid system comprises a diluent bucket, first to fifth three-way valves, first to third two-way valves, first to third driving devices, a sheath flow injector, first to third three-way tubes, a flowing chamber, a sample flow injector, a blood sampling injector, a blood sampling needle, a reaction tank, a hemolytic agent injector, a hemolytic agent bottle and a blood sampling needle washing inlet. According to the fluid system of the flow cytometry, the sample flow injector and the blood sampling injector are driven by virtue of one same driving device, so that a driving device is omitted, the structure is simplified, the cost of the system is lowered, and as the two injectors share one same driving device, the overall rigidity and the inertia are improved, the instability of gaps between the driving devices can be buffered, and the stability of a sample flow is improved.

Description

A kind of fluid system of flow cytometer
[technical field]
The utility model relates to a kind of fluid system, is specifically related to a kind of fluid system of flow cytometer.
[background technology]
Flow cytometer is to control cell one by one by the blood analysis instrument of detection zone by flow cytometry, flow cytometry refers to that stream of cells (also referred to as sample flow) is under the parcel of sheath fluid, cell is queued up by the fluid control technology of flow chamber detection zone, this technology can avoid cell by irregular route, through detection zone, to cause the problem that detects misalignment, can greatly reduce cell overlap and produce undetected probability by detection zone simultaneously.In prior art, the fluid system of flow cytometer mainly divides two classes: the first kind is that sheath stream and sample flow all adopt syringe to push away sample, each syringe needs to configure separately a motor and gearing and drives, due to motor and supporting gearing holistic cost higher, so high expensive of this scheme; Equations of The Second Kind is that sample flow is used syringe to push away sample, and sheath stream is used constant voltage source of the gas to push away sample, and constant voltage source of the gas drives sheath stream to need a set of constant voltage air-channel system in addition, and cost is very high, and simultaneity factor complex structure is not easy to safeguard.In addition,, because flow cytometer is high to the stability requirement of sample flow, therefore high to the rigidity of syringe and inertia requirement, batch difficult quality of material is controlled.
[summary of the invention]
For above-mentioned defect, the utility model discloses a kind of fluid system of flow cytometer, its cost is low, simple in structure, simultaneously the good stability of sample flow.
The technical solution of the utility model is as follows:
A fluid system for flow cytometer, comprises that dilution bucket, the first to the 5th T-valve, the first to the 3rd two-port valve, the first to the 3rd drive unit, sheath streamer emitter, the first to the 3rd three-way pipe, flow chamber, sample flow syringe, injector for collecting blood, blood taking needle, reaction tank, hemolytic agent syringe, hemolytic agent bottle, blood taking needle clean entrance;
Described dilution bucket is connected with the Chang Kaiduan of the first T-valve, the normal-closed end of the first T-valve is connected with the normal-closed end of the first two-port valve, the first end of common port first three-way pipe of the first T-valve connects, the second end of the first three-way pipe is connected with the outlet of sheath streamer emitter, the 3rd end of the first three-way pipe is connected with the normal-closed end of the second two-port valve, and the first drive unit is connected with the piston of sheath streamer emitter, the normal-closed end of the first two-port valve is connected with flow chamber, the normal-closed end of the second two-port valve is connected with the first end of the second three-way pipe, the second end of the second three-way pipe is connected with flow chamber, the 3rd end of the second three-way pipe is connected with the first end of the 3rd three-way pipe, the second end of the 3rd three-way pipe is connected with the Chang Kaiduan of the 3rd two-port valve, the Chang Kaiduan of the 3rd two-port valve is connected with reaction tank, the 3rd end of the 3rd three-way pipe is connected with the outlet of sample flow syringe, the piston of sample flow syringe is connected with the second drive unit with the piston of injector for collecting blood, the outlet of injector for collecting blood is connected with the common port of the 4th T-valve, the exit of injector for collecting blood is also provided with dilution entrance, the Chang Kaiduan of the 4th T-valve cleans entrance with blood taking needle and is connected, the normal-closed end of the 4th T-valve is connected with blood taking needle, the normal-closed end of the 5th T-valve is connected with reaction tank, and the Chang Kaiduan of the 5th T-valve is connected with hemolytic agent bottle, and the common port of the 5th T-valve is connected with the outlet of hemolytic agent syringe, and the piston of hemolytic agent syringe is connected with the 3rd drive unit.
In the fluid system of flow cytometer of the present utility model, sample flow syringe and injector for collecting blood drive by same drive unit, reduced a set of drive unit, simplify the structure, reduced the cost of system, two syringes share same drive unit simultaneously, and whole rigidity and inertia increase, the gap that can cushion drive unit is stationarity not, thereby has improved the stationarity of sample flow.
[accompanying drawing explanation]
Fig. 1 is the structural representation of the utility model embodiment;
Fig. 2 is the structural representation of the utility model one preferred embodiment.
[embodiment]
Below in conjunction with accompanying drawing, embodiment of the present utility model is elaborated.
As shown in Figure 1 to Figure 2, the fluid system of flow cytometer of the present utility model comprises that dilution bucket 1, the first T-valve 2, the first two-port valve 3, the first drive unit 4, sheath streamer emitter 5, the first three-way pipe 6, the second two-port valve 7, the second three-way pipe 8, flow chamber 9, the second drive unit 10, sample flow syringe 11, the 3rd three-way pipe 12, injector for collecting blood 13, the 3rd two-port valve 14, blood taking needle 15, the 4th T-valve 16, reaction tank 17, the 3rd drive unit 18, hemolytic agent syringe 19, the 5th T-valve 20, hemolytic agent bottle 21, sampling needle clean entrance 22;
Described dilution bucket 1 is connected with the Chang Kaiduan of the first T-valve 2, the normal-closed end of the first T-valve 2 is connected with the normal-closed end of the first two-port valve 3, the first end of common port first three-way pipe 6 of the first T-valve 2 connects, the second end of the first three-way pipe 6 is connected with the outlet of sheath streamer emitter 5, the 3rd end of the first three-way pipe 6 is connected with the normal-closed end of the second two-port valve 7, and the first drive unit 4 is connected with the piston of sheath streamer emitter 5, the normal-closed end of the first two-port valve 3 is connected with flow chamber 9, the normal-closed end of the second two-port valve 7 is connected with the first end of the second three-way pipe 8, the second end of the second three-way pipe 8 is connected with flow chamber 9, the 3rd end of the second three-way pipe 8 is connected with the first end of the 3rd three-way pipe 12, the second end of the 3rd three-way pipe 12 is connected with the Chang Kaiduan of the 3rd two-port valve 14, the Chang Kaiduan of the 3rd two-port valve 14 is connected with reaction tank 17, the 3rd end of the 3rd three-way pipe 12 is connected with the outlet of sample flow syringe 11, the piston of the piston of sample flow syringe 11 and injector for collecting blood 13 is connected with the second drive unit 10, the outlet of injector for collecting blood 13 is connected with the common port of the 4th T-valve 16, the exit of injector for collecting blood 13 is also provided with dilution entrance 23, the Chang Kaiduan of the 4th T-valve 16 cleans entrance 22 with sampling needle and is connected, the normal-closed end of the 4th T-valve 16 is connected with blood taking needle 15, the normal-closed end of the 5th T-valve 20 is connected with reaction tank 17, and the Chang Kaiduan of the 5th T-valve 20 is connected with hemolytic agent bottle 21, and the common port of the 5th T-valve is connected with the outlet of hemolytic agent syringe 19, and the piston of hemolytic agent syringe 19 is connected with the 3rd drive unit 18.
The fluid system working method of flow cytometer of the present utility model is as follows: 1) the 4th T-valve 16 and the second two-port valve 7 actions, the second drive unit 10 drives sample flow syringe 11 and injector for collecting blood 13 work, injector for collecting blood 13 is taken a blood sample by blood taking needle 15, and the path that sample flow syringe 11 consists of the 3rd three-way pipe 12, the second three-way pipe 8, the second two-port valve 7, the first three-way pipe 6, the first T-valve 2 is inhaled dilution from dilution bucket 1; 2) blood taking needle 15 enters reaction tank 17, the second drive units 10 and drives injector for collecting bloods 13 to separate specified blood sample to enter reaction tank 17; 3) the 3rd drive unit 18 drives hemolytic agent syringe 19 to suck hemolytic agent from hemolytic agent bottle, then the 5th T-valve 20 actions, and the 3rd drive unit 18 drives hemolytic agent syringe 19, hemolytic agent is sent into reaction tank and react with blood sample; 4) the first drive unit 4 drives 5 work of sheath streamer emitter, and sheath streamer emitter 5 is by the complete blood sample of path sucting reaction consisting of the first three-way pipe 6, the second two-port valve 7, the second three-way pipe 8, the 3rd three-way pipe 12 and the 3rd two-port valve 14; 5) the first T-valve 2, the first two-port valve 3, the 3rd two-port valve 14 actions, the second two-port valve 7, the 4th T-valve 16 reset, the first drive unit 4 drives sheath streamer emitter 5 that blood sample is sent into flow chamber 9, the second drive unit 10 drives sample flow syringe 11 that dilution is sent into flow chamber 9 simultaneously, parcel blood sample is queued up and is carried out blood cell analysis detection by flow chamber 9, and the dilution that injector for collecting blood 13 sucks dilution entrance 23 is pushed into blood taking needle and cleans entrance to clean blood taking needle 19.
In the fluid system of flow cytometer of the present utility model, sample flow syringe 11 and injector for collecting blood 13 drive by same drive unit, reduced a set of drive unit, simplify the structure, reduced the cost of system, two syringes share same drive unit simultaneously, make to be carried in the mass penalty on drive unit, thereby increased whole inertia, two syringes increase with respect to the rigidity of single syringe, the gap that can cushion drive unit is stationarity not, thereby has improved the stability of sample flow.
Further, the promotion resistance of sample flow syringe 11 equals the promotion resistance of injector for collecting blood 13 with respect to the moment of resistance at the second drive unit 10 center with respect to the moment of resistance at the second drive unit 10 center, for example, the promotion resistance of sample flow syringe 11 is F1, sample flow syringe 11 is L1 with respect to the distance at the second drive unit 10 center, the promotion resistance of injector for collecting blood 13 is F2, injector for collecting blood 13 is L2 with respect to the distance at the second drive unit 10 center, F1, L1, F2, L2 meets and is related to F1 * L1=F2 * L2, meet equalising torque, therefore can improve the serviceable life that drives stationarity and drive unit.Simultaneously, due to the existence of two syringes, integral rigidity and inertia increase, and the gap that can cushion drive unit is stationarity not, obtains than driving single syringe scheme sample flow more stably, thereby improves the accuracy of detection of flow cytometer;
Further, described the first drive unit 4 or the second drive unit 10 or the 3rd drive unit 18 comprise stepper motor and gear train, adopt stepper motor can control flexibly rotating speed and turn to, fast response time, and degree of regulation is high, is convenient to the robotization of system;
Further, described gear train is leading screw, by leading screw, rotatablely moving of stepper motor can be converted into rectilinear motion, and then the piston movement of pushing syringe, and it is simple in structure, transmits motion reliably;
Preferably, described the first T-valve 2 or the second T-valve 8 or the 3rd T-valve 12 or the 4th T-valve 16 or the 5th T-valve 20 are two position, three-way electromagnetic change valve, adopt solenoid directional control valve to be conducive to the robotization of the system that realizes;
Preferably, described the first two-port valve 3 or the second two-port valve 7 or the 3rd two-port valve 14 are bi-bit bi-pass solenoid directional control valve, so that the robotization of the system of realization is controlled;
Preferably, the fluid system of flow cytometer of the present utility model also comprises the second hemolytic agent syringe 24, the 6th T-valve 25, the second hemolytic agent bottle 26; The piston of described the second hemolytic agent syringe 24 is connected with the 3rd drive unit 18, the outlet of the second hemolytic agent syringe 24 is connected with the common port of the 6th T-valve 25, the normal-closed end of the 6th T-valve 25 is connected with reaction tank 17, and the Chang Kaiduan second hemolytic agent bottle of the 6th T-valve 25 connects; The action of the 6th T-valve 25 or reset identically with the 5th T-valve 20 in work, the second hemolytic agent syringe 24 is sent into the another kind of hemolytic agent in the second hemolytic agent bottle 26 in reaction tank 17 and is reacted with blood sample, the reaction effect of reinforcement blood sample and hemolytic agent;
Further, described the 6th T-valve 25 is two position, three-way electromagnetic change valve.
Above-described embodiment of the present invention, does not form limiting the scope of the present invention.Any modification of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in claim protection domain of the present invention.

Claims (8)

1. a fluid system for flow cytometer, is characterized in that: comprise that dilution bucket, the first to the 5th T-valve, the first to the 3rd two-port valve, the first to the 3rd drive unit, sheath streamer emitter, the first to the 3rd three-way pipe, flow chamber, sample flow syringe, injector for collecting blood, blood taking needle, reaction tank, hemolytic agent syringe, hemolytic agent bottle, blood taking needle clean entrance;
Described dilution bucket is connected with the Chang Kaiduan of the first T-valve, the normal-closed end of the first T-valve is connected with the normal-closed end of the first two-port valve, the common port of the first T-valve is connected with the first end of the first three-way pipe, the second end of the first three-way pipe is connected with the outlet of sheath streamer emitter, the 3rd end of the first three-way pipe is connected with the normal-closed end of the second two-port valve, and the first drive unit is connected with the piston of sheath streamer emitter, the normal-closed end of the first two-port valve is connected with flow chamber, the normal-closed end of the second two-port valve is connected with the first end of the second three-way pipe, the second end of the second three-way pipe is connected with flow chamber, the 3rd end of the second three-way pipe is connected with the first end of the 3rd three-way pipe, the second end of the 3rd three-way pipe is connected with the Chang Kaiduan of the 3rd two-port valve, the Chang Kaiduan of the 3rd two-port valve is connected with reaction tank, the 3rd end of the 3rd three-way pipe is connected with the outlet of sample flow syringe, the piston of sample flow syringe is connected with the second drive unit with the piston of injector for collecting blood, the outlet of injector for collecting blood is connected with the common port of the 4th T-valve, the exit of injector for collecting blood is also provided with dilution entrance, the Chang Kaiduan of the 4th T-valve cleans entrance with blood taking needle and is connected, the normal-closed end of the 4th T-valve is connected with blood taking needle, the normal-closed end of the 5th T-valve is connected with reaction tank, and the Chang Kaiduan of the 5th T-valve is connected with hemolytic agent bottle, and the common port of the 5th T-valve is connected with the outlet of hemolytic agent syringe, and the piston of hemolytic agent syringe is connected with the 3rd drive unit.
2. the fluid system of flow cytometer as claimed in claim 1, is characterized in that: the promotion resistance of described sample flow syringe equals the promotion resistance of injector for collecting blood with respect to the moment of resistance at the center of the second drive unit with respect to the moment of resistance at the center of the second drive unit.
3. the fluid system of flow cytometer as claimed in claim 1 or 2, is characterized in that: described the first drive unit, the second drive unit or the 3rd drive unit comprise stepper motor and gear train.
4. the fluid system of flow cytometer as claimed in claim 3, is characterized in that: described gear train is leading screw.
5. the fluid system of flow cytometer as claimed in claim 1 or 2, is characterized in that: described the first T-valve, the second T-valve, the 3rd T-valve, the 4th T-valve or the 5th T-valve are two position, three-way electromagnetic change valve.
6. the fluid system of flow cytometer as claimed in claim 1 or 2, is characterized in that: described the first two-port valve, the second two-port valve or the 3rd two-port valve are bi-bit bi-pass solenoid directional control valve.
7. the fluid system of flow cytometer as claimed in claim 1 or 2, is characterized in that: also comprise the second hemolytic agent syringe, the 6th T-valve, the second hemolytic agent bottle; The piston of described the second hemolytic agent syringe is connected with the 3rd drive unit, the outlet of the second hemolytic agent syringe is connected with the common port of the 6th T-valve, the normal-closed end of the 6th T-valve is connected with reaction tank, and the Chang Kaiduan of the 6th T-valve is connected with the second hemolytic agent bottle.
8. the fluid system of flow cytometer as claimed in claim 7, is characterized in that: described the 6th T-valve is two position, three-way electromagnetic change valve.
CN201320602437.3U 2013-09-27 2013-09-27 Fluid system of flow cytometry Expired - Fee Related CN203561572U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201320602437.3U CN203561572U (en) 2013-09-27 2013-09-27 Fluid system of flow cytometry

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201320602437.3U CN203561572U (en) 2013-09-27 2013-09-27 Fluid system of flow cytometry

Publications (1)

Publication Number Publication Date
CN203561572U true CN203561572U (en) 2014-04-23

Family

ID=50511287

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201320602437.3U Expired - Fee Related CN203561572U (en) 2013-09-27 2013-09-27 Fluid system of flow cytometry

Country Status (1)

Country Link
CN (1) CN203561572U (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103994962A (en) * 2014-04-25 2014-08-20 深圳市帝迈生物技术有限公司 Fluid system of flow cytometer and flow cytometric detection method
CN104127192A (en) * 2013-10-16 2014-11-05 深圳市帝迈生物技术有限公司 Fluid system of flow cytometer and flow cell detecting method
CN104458544A (en) * 2014-12-29 2015-03-25 中国科学院长春光学精密机械与物理研究所 Liquid flow system of flow cytometer
CN104897557A (en) * 2015-05-30 2015-09-09 广州埃克森生物科技有限公司 Flow cytometry detection fluid circuit system and flow cytometry detection method
CN104914031A (en) * 2015-06-12 2015-09-16 广州埃克森生物科技有限公司 Flow cytometry fluid path system and flow cytometry method
CN105717034A (en) * 2016-02-01 2016-06-29 深圳开立生物医疗科技股份有限公司 Liquid path system and flow cytometry detection method thereof
CN105784571A (en) * 2016-02-29 2016-07-20 深圳市帝迈生物技术有限公司 Double-cell measurement method and device for certain reaction protein (CRP)
CN107727557A (en) * 2017-10-16 2018-02-23 广州竞天生物科技有限公司 A kind of particle analyzer and its liquid-way system and detection control method
CN108627449A (en) * 2017-03-23 2018-10-09 深圳市帝迈生物技术有限公司 A kind of flow cytometer fluid system and measurement method
CN109211737A (en) * 2017-06-30 2019-01-15 深圳迈瑞生物医疗电子股份有限公司 Sample testing apparatus, sample analyser and sample testing method

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104127192B (en) * 2013-10-16 2016-08-10 深圳市帝迈生物技术有限公司 A kind of flow cytometer fluid system and FCM analysis method
CN104127192A (en) * 2013-10-16 2014-11-05 深圳市帝迈生物技术有限公司 Fluid system of flow cytometer and flow cell detecting method
CN103994962A (en) * 2014-04-25 2014-08-20 深圳市帝迈生物技术有限公司 Fluid system of flow cytometer and flow cytometric detection method
CN103994962B (en) * 2014-04-25 2016-08-24 深圳市帝迈生物技术有限公司 A kind of flow cytometer fluid system and FCM analysis method
CN104458544A (en) * 2014-12-29 2015-03-25 中国科学院长春光学精密机械与物理研究所 Liquid flow system of flow cytometer
CN104458544B (en) * 2014-12-29 2017-04-05 中国科学院长春光学精密机械与物理研究所 A kind of liquid fluid system of flow cytometer
CN104897557A (en) * 2015-05-30 2015-09-09 广州埃克森生物科技有限公司 Flow cytometry detection fluid circuit system and flow cytometry detection method
CN104897557B (en) * 2015-05-30 2018-03-09 广州埃克森生物科技有限公司 FCM analysis liquid-way system and FCM analysis method
CN104914031A (en) * 2015-06-12 2015-09-16 广州埃克森生物科技有限公司 Flow cytometry fluid path system and flow cytometry method
CN105717034A (en) * 2016-02-01 2016-06-29 深圳开立生物医疗科技股份有限公司 Liquid path system and flow cytometry detection method thereof
CN105784571A (en) * 2016-02-29 2016-07-20 深圳市帝迈生物技术有限公司 Double-cell measurement method and device for certain reaction protein (CRP)
CN108627449A (en) * 2017-03-23 2018-10-09 深圳市帝迈生物技术有限公司 A kind of flow cytometer fluid system and measurement method
CN108627449B (en) * 2017-03-23 2023-03-31 深圳市帝迈生物技术有限公司 Flow cytometer fluid system and measuring method
CN109211737A (en) * 2017-06-30 2019-01-15 深圳迈瑞生物医疗电子股份有限公司 Sample testing apparatus, sample analyser and sample testing method
CN107727557A (en) * 2017-10-16 2018-02-23 广州竞天生物科技有限公司 A kind of particle analyzer and its liquid-way system and detection control method

Similar Documents

Publication Publication Date Title
CN203561572U (en) Fluid system of flow cytometry
CN104127192B (en) A kind of flow cytometer fluid system and FCM analysis method
CN104914031A (en) Flow cytometry fluid path system and flow cytometry method
CN103994962B (en) A kind of flow cytometer fluid system and FCM analysis method
CN103389386B (en) Portable three-differential blood cell analyzer liquid path system and method thereof
CN104297108B (en) Particle analyzer and its liquid-way system
CN104749085B (en) Particle Analyzer Fluid Flow System and Particle Analyzer
US10288545B2 (en) Fluidics system for flow cytometer
CN108169104A (en) Flow cytometry detection device and method
CN103852587B (en) Liquid sample introduction system and control method thereof
CN103712902B (en) Adopt the cellanalyzer of single negative pressure source and pipeline flow regulator
CN101869894B (en) Clean device, purging system, cleaning method and particle analyzer
WO2013155288A1 (en) Particle dispensing apparatus and method
CN108732081A (en) A kind of immunocyte instrument fluid system
CN102559486A (en) Full-automatic air microbe sampler
CN203397138U (en) Liquid channel control system of full-automatic enzyme immunoassay analyzer
CN101581565B (en) Piston stroke detecting method for electronic pipettor and special device
CN104730216B (en) A kind of analysis metering device and liquid analysis system
CN104764860B (en) A kind of analysis metering device and liquid analysis system
CN203881786U (en) Liquid sample injection system
CN112345433A (en) A liquid path system and blood cell analyzer thereof
CN108627449A (en) A kind of flow cytometer fluid system and measurement method
CN202974750U (en) Separation and enrichment system
CN203759033U (en) Sampling arm for automatic pipe type chemiluminescent analyzer
CN202974872U (en) Steam generation and steam-liquid separation system

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140423

Termination date: 20140927

EXPY Termination of patent right or utility model