CN203521365U - Electrospray ionization device - Google Patents
Electrospray ionization device Download PDFInfo
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- CN203521365U CN203521365U CN201320412840.XU CN201320412840U CN203521365U CN 203521365 U CN203521365 U CN 203521365U CN 201320412840 U CN201320412840 U CN 201320412840U CN 203521365 U CN203521365 U CN 203521365U
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- electron spray
- tinsel
- spray ionisation
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Abstract
The utility model relates to an electrospray ionization device, which comprises a voltage interface (1) and a metal foil (2), wherein the voltage interface (1) is used for providing ionization high voltage, the metal foil (2) is connected to the voltage interface (1) and used for loading an ionization sample (6) and exciting the ionization sample (6) by using the ionization high voltage to carry out ionization and form spray ions (8).
Description
Technical field
The utility model relates to analysis technical field, is specifically related to electron spray ionisation device.
Background technology
Electrospray ionization mass spectrometry (ESI-MS) belongs to a kind of of soft ionization mode, under certain voltage, by measuring the mass-charge ratio (M/Z) of sample component, detect the molecular weight of sample component, qualitative, quantitative to materials such as polypeptide, protein and oligonucleotides, and can to mixture, analyze with high performance liquid chromatograph coupling.This technology taught by the John Fenn of Yale the detection that developed and be applied to large biological molecule in 1988, and therefore John professor Fenn has obtained Nobel chemistry Prize in 2002
In existing electron spray ionisation device and method, general employing is used as ion source with the electron spray capillary of high pressure, the sample that contains polar solvent is set in capillary, by apply voltage at capillary end, introduce sample and sample is carried out to ionization, yet existing capillary ion source only can be introduced solution example, and due to its shape restriction, in the introducing of solution example and ionization process, be easy to produce the problem that capillary stops up, be subject to the restriction of material capillaceous simultaneously, the process more complicated that sample is introduced and can only carry out ionization to sample, and cannot before ionization, to sample, carry out pretreatment operation.
Utility model content
The purpose of this utility model is the sample that can introduce in ionization process for existing electron spray ionisation device problem single, that easily stop up and cannot carry out sample pretreatment, and a kind of sample that is applicable to variform, simple in structure and be convenient to sample to carry out pretreated electron spray ionisation device is provided.
The technical scheme that the utility model provides with regard to above-mentioned technical problem is as follows: construct a kind of electron spray ionisation device, comprise voltage interface and the tinsel being connected with described voltage interface;
Described voltage interface is for applying voltage to described tinsel;
Described tinsel is used for carrying testing sample and utilizes described voltage to excite described testing sample that spraying ion occurs to ionize and produce.
In electron spray ionisation device of the present utility model, described tinsel comprises the spout that sprays described spraying ion voltage interface for orientation.
In electron spray ionisation device of the present utility model, described tinsel comprises for carrying the main body of described testing sample, and two barrier plates that form an angle with described main body, and described spout is formed between described two barrier plates.
In electron spray ionisation device of the present utility model, described main body is pentagon, comprise base, vertical with base two sides and respectively with the flanging of two side obliques, article two, flanging is vertically turned up to the same side and is formed described two barrier plates, between described two barrier plates, offers breach to form described spout.
In electron spray ionisation device of the present utility model, described electron spray ionisation device also comprises the support for tinsel described in fixed support, and described tinsel is made by chemical inertness metal.
In electron spray ionisation device of the present utility model, described tinsel thickness is 10～100 microns.
In electron spray ionisation device of the present utility model, the described angle that described two barrier plates of described tinsel and described main body form is 10～90 °.
In electron spray ionisation device of the present utility model, the width of described spout is 50～500 microns.
In electron spray ionisation device of the present utility model, described electron spray ionisation device also comprises the heater being arranged near described tinsel below, for described testing sample is heated.
The electron spray ionisation device that the utility model provides, by using tinsel, as ionization source, introduce sample and on this tinsel, carry out sample pretreatment, the capillary that efficiently solves prior art easily stops up and can only introduce solution example and can not carry out pretreated problem to sample, has wide market application foreground.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is described in further detail, in accompanying drawing:
Fig. 1 is the electron spray ionisation apparatus structure schematic diagram of the first preferred embodiment of the present utility model;
Fig. 2 is the electron spray ionisation apparatus structure schematic diagram of the second preferred embodiment of the present utility model;
Fig. 3 is the mass spectrogram that uses the liquid red drink heavily material of electron spray ionisation device analysis of the present utility model;
Fig. 4 is for being used the mass spectrogram of the electron spray ionisation device analysis thickness state terbinafine HCl ointment of utility model;
The mass spectrogram of Fig. 5 for using electron spray ionisation device of the present utility model to extract Ginseng Root Powder and detect;
Fig. 6 A is for being used electron spray ionisation device of the present utility model to detect the mass spectrogram that contains highly-saline lysozyme soln
Fig. 6 B is for adopting the mass spectrogram of electron spray ionisation device of the present utility model to lysozyme desalination detection
Fig. 7 A is for adopting electron spray ionisation device of the present utility model to detect the mass spectrogram of myoglobins solution in the time of 20 ℃
Fig. 7 B is for adopting electron spray ionisation device of the present utility model to detect the mass spectrogram of myoglobins solution when being heated to 74 ℃ of temperature
Fig. 7 C is for adopting electron spray ionisation device of the present utility model to detect the mass spectrogram of myoglobins solution when being heated to 85 ℃
Fig. 8 A adopts electron spray ionisation device of the present utility model at the mass spectrometry results figure to organic compound reserpine reserpine;
Fig. 8 B is the Gly-Val-Phe(glutamic acid-Ile-Phe of electrospray ionization mass spectrometry system acquisition of the present utility model) the mass spectrometry results figure of polypeptide sample;
Fig. 8 C is the mass spectrometry results figure of the myoglobins aqueous sample of electrospray ionization mass spectrometry system acquisition of the present utility model;
The mass spectrogram of Fig. 9 A for adopting electron spray ionisation device of the present utility model to detect the gramicidins that contains activating agent;
The mass spectrogram of Fig. 9 B for adopting electron spray ionisation device of the present utility model also to detect except activating agent gramicidins.
Embodiment
With reference to figure 1, it is the first preferred embodiment of electron spray ionisation device of the present utility model.In the present embodiment, described electron spray ionisation device and mass spectrometer coupling are formed to a kind of electrospray ionization mass spectrometry analytical system, wherein said electron spray ionisation device comprises voltage interface 1 and the tinsel 2 being connected with voltage interface 1.
In the present embodiment, described electron spray ionisation device and mass spectrometer are to coupling, and described mass spectrometer comprises the injection port 5 with described tinsel 2 corresponding settings, for receiving described spraying ion 8 and carrying out mass spectral analysis.
Further, described tinsel 2 comprises spout 4, and described spout 4 is towards mass spectrometer injection port 5, for spraying described spraying ion 8 to described mass spectrometer injection port 5.Especially, 5～12 millimeters of mass spectrometer injection ports described in described jet opening distance.
As shown in Figure 1, tinsel 2 comprises the main body (not marking) for carrying described testing sample 6, and two barrier plates 3 that form an angle with described main body.Described spout 4 is formed between described two barrier plates 3.
Especially, described main body is pentagon, comprise base, vertical with base two sides and respectively with the flanging of two side obliques, two flangings are vertically turned up to the same side and form described two barrier plates 3, offer breach to form described spout 4 between described two barrier plates 3.
The shape that should be appreciated that tinsel 2 can be also other rule or irregular geometry, such as rhombus, triangle etc.Barrier plate 3 is set in one end of tinsel 2 and forms spout 4.
Barrier plate 3 and the angle between main body of tinsel 2 are 10～90 °.Described tinsel thickness is preferably 10～100 microns.The openings of sizes of the spout 4 that the barrier plate 3 that described tinsel front end is turned up forms is 50～500 microns.It is upper that testing sample 6 is placed in tinsel 2 surfaces, the rear end of voltage interface 1 direct connection metal paillon foil 2, and spout 4 is being aimed at mass spectrometer injection port 5, and after high-tension electricity 1 is connected, testing sample formation spraying ion 8 enters mass spectrometer injection port 5 and analyzes.
As shown in Figure 2, the second embodiment for electron spray ionisation device of the present utility model, compare with the first embodiment, described electron spray ionisation device also comprises the support 9 for tinsel described in fixed support 2, also comprise the heater 7 being arranged near described tinsel 2 belows, for described sample 6 is heated.Heater 7 is positioned at 1～20 millimeter of place under tinsel 2, in the time of need to heat-treating sample 6, can open heater 7, by regulating the temperature of heater 7 or regulating the temperature of the distance adjustment sample 6 between heater 7 and tinsel 2.
Adopt above-mentioned electron spray ionisation device of the present utility model, creatively use tinsel 2 carrying determinand samples 6, because chemical inertness, surface cleaning, the heat conduction of tinsel 2 are good, easily machine-shaping, can realize several functions such as the carrying of sample 6, extraction, crystallization, desalination (except surfactant), heating.Due to the good electric conductivity of tinsel 2, there is ionization in testing sample 6 in high voltage electric field, produces the ion of testing sample 6, can enter the mass spectrometer being coupled with described electron spray ionisation device and detect.Electron spray ionisation device of the present utility model, compact conformation, design ingenious, easy to use, can for various detection samples 6, carry out sample treatment and mass spectral analysis flexibly, not only there is high sensitivity and high specific, and the sample of variform (as liquid state, thickness state, moistening solid) can direct ionization, follow the preliminary treatment (as extraction, crystallization, heating) that can realize various ways.Therefore, this electron spray ionisation device of the present utility model is particularly suitable for complicated biological sample, food, medicine, environmental sample etc. to carry out rapid analysis.
The utility model discloses a kind of electron spray ionisation method of using above-mentioned electron spray ionisation device.Building block below in conjunction with above-mentioned electron spray ionisation device is set forth electron spray ionisation method of the present utility model.
Use above-mentioned electron spray ionisation device, can realize the direct electron spray ionisation of liquid towards, thickness state, moistening solid (as biological tissue). below in conjunction with using the method for above-mentioned electron spray ionisation device to be described electron spray ionisation device of the present utility model, the method comprises:
Step 1: testing sample 6 is placed on the tinsel 2 of above-mentioned electron spray ionisation device, on the tinsel 2 of described electron spray ionisation device, described testing sample 6 is carried out to preliminary treatment;
Step 2: apply high-tension electricity to described tinsel, 2 pairs of described samples 8 of described tinsel ionize and produce and spray described spraying ion 8.
Especially, when described electron spray ionisation device and mass spectrometer are coupled for mass spectral analysis, above-mentioned steps two further comprises: from described tinsel 2, described spraying ion 8 is ejected into mass spectrometric injection port 5, opens described mass spectrometer and carry out mass spectral analysis.
Described testing sample 6 comprises: fluid sample, powder sample, toughness sample, drying solid sample or moistening solid sample.
Described preliminary treatment comprises that auxiliary extraction, auxiliary electrical are from, surperficial desalination or auxiliary heating
When described testing sample 6 is powder or drying solid sample, described method also comprises: to described testing sample, apply solvent and extract or dissolve.
When testing sample shown in described 6 is liquid, toughness or moistening solid sample, described method also comprises: apply solvent and assist ionization; By the described electron spray ionisation device described mass spectrometer that is coupled, described voltage interface 1 directly connects described tinsel 2 provides ionization high pressure;
Described testing sample 6 for to contain salt/during the protein/polypeptide sample of surfactant, described step 1 comprises:
Described testing sample on described electron spray ionisation device 6 is unlimited air-dry, treat salinity or surfactant spontaneous nucleation; The frozen water that absorbs zero degrees celsius by pipettor leach described crystallization salinity or surfactant, dissolve, siphon away;
To adding again solvent in residual described protein or polypeptide sample, dissolve.
In above-mentioned mass spectrometric analysis method, voltage interface described in step 21 imposes 2～5 kilovolts of high-tension electricity ionization to described tinsel 2 and makes sample.Preferred ionization high pressure is 3.5kV.
Particularly, the mass spectral analysis process below in conjunction with the detection sample of different shape is described further electron spray ionisation method of the present utility model.
Use electron spray ionisation device of the present utility model to detect solid sample and the electron spray ionisation method of mass spectral analysis comprises:
Step 1: by the above tinsel 2 and be connected with high-tension electricity, spout 4 is aimed at mass spectrometer injection ports 5, and the spout 4 of tinsel 2 is 5～12 millimeters apart from mass spectrum injection port;
Step 2: the one end that liquid state to be measured, thickness state or moistening solid sample 6 is placed in to close described spout 4 on tinsel;
Step 3: make sample 6 ionization to imposing 2～5 kilovolts of high-tension electricities on tinsel 2 by described voltage interface 1
Step 4: open mass spectrometer scanning system, obtain testing result.
When needs auxiliary extraction or auxiliary electrical from time, in step 2, can add as required appropriate reagent simultaneously.
In the time of needs auxiliary heating, in step 2, can use as required heater 7 to heat.
The high-tension electricity of mentioning in above method, optional positive voltage or negative voltage.
Use electron spray ionisation device of the present utility model to comprise the following steps the electrospray ionization mass spectrometry analytical method of powder or dry solid sample:
Step 1: the above tinsel 2 is fixed and is connected with high-tension electricity, and front end is aimed at mass spectrometer injection port 5, and the spout 4 of tinsel 2 is 5～12 millimeters apart from mass spectrometer injection port 5;
Step 2: powder to be measured or dry solid sample 6 are placed on tinsel 2 to the one end near spout;
Step 3: choose the reagent needing and be added on powder to be measured or dry solid sample 6 and carry out solvent extraction effect;
Step 4: impose 2～5 kilovolts of high-tension electricities to tinsel 2 and make sample 6 ionization;
Step 5: open mass spectrometer scanning system, obtain testing result.
In the time of needs auxiliary heating, in step 3, can use as required heater to heat.
The high-tension electricity of mentioning in above method, optional positive voltage or negative voltage.
In addition,, during for protein (or polypeptide) sample of contain salt (or surfactant), need to carry out desalination (or removing surfactant) and MALDI-MS analysis.The method comprises the following steps:
Step 1: protein (or polypeptide) solution example 6 of contain salt (or surfactant) is placed on the surface of tinsel 2.
Step 2: the tinsel 2 that is equipped with sample 6 is air-dry in air, treat salinity (or surfactant) spontaneous nucleation.
Step 3: the crystal of the frozen water absorbing by pipettor (zero degrees celsius) leaching salinity (or surfactant), dissolves and siphons away.
Step 4: will contain except the spout 4 of the tinsel 2 of protein (or polypeptide) sample 6 of freshen (or surfactant) and aim at mass spectrum injection ports, spout 4 and the distance between mass spectrometer injection port 5 of tinsel 2 are 5～12 millimeters.
Step 5: add again described in other agent dissolves the protein (or polypeptide) except freshen (or surfactant).
Step 6: impose 2～5 kilovolts of high-tension electricities by voltage interface 1 to tinsel 2 and make sample ionization.
Step 7: open mass spectrometer scanning system, obtain testing result.
In the time of needs auxiliary heating, in step 5, can use as required heater to heat simultaneously.
The high-tension electricity of mentioning in above method, optional positive voltage or negative voltage.
Below in conjunction with the concrete sample that detects, describe application of the present utility model in detail, by electron spray ionisation device of the present utility model, heating to complicated substrate sample beverage, toughness ointment, samples of Ginseng powder, the protein solution containing salt and protein sample, has obtained good result.
the direct-detection of standard liquid sample
With reference to figure 8, similar with existing capillary electron spray ionisation source, use the solution example that the utility model electron spray ionisation device also can direct-detection standard, as organo-metallic compound, organic compound, polypeptide, protein etc.Fig. 8 A represents the mass spectrometry results of the organic compound reserpine of electrospray ionization mass spectrometry system acquisition of the present utility model, and wherein, detecting sample reserpine is that 10 microlitre concentration are the solution of 0.1 mcg/ml, and solvent is 1:1(volume ratio) water and methyl alcohol.Fig. 8 B represents the Gly-Val-Phe(glutamic acid-Ile-Phe of electrospray ionization mass spectrometry system acquisition of the present utility model) mass spectrometry results of polypeptide sample, wherein detect sample Gly-Val-Phe(glutamic acid-Ile-Phe) polypeptide volume is that 10 microlitres, concentration are the aqueous solution of 0.3 micromoles per liter.Fig. 8 C represents that the volume of electrospray ionization mass spectrometry system acquisition of the present utility model is 10 microlitres, and concentration is the mass spectrometry results of the myoglobins aqueous sample of 10 micromoles per liter.The assessment that Fig. 8 A-Fig. 8 C is confirmatory use the repeatability of the electrospray ionization mass spectrometry analytical method of the utility model electron spray ionisation device.
the direct-detection of liquid state and thickness complex sample
The capillary adopting in existing electrospray ionization mass spectrometry analytical equipment has susceptible to plugging shortcoming, mostly can not detect the sample of toughness.
The utility model utilizes the easy process and remould character of metal and good conductivity, uses tinsel to have certain degree of hardness, and plasticity also can be carried sample.The utility model is used tinsel electrospray ionization mass spectrometry Direct Analysis complex sample, realizes the Direct Analysis of sample is detected.
With reference to figure 3, first utilize and adopted the electron spray ionisation device of metal aluminium foil to carry out quick mass spectral analysis to the red ox of drinks, method is: the red drink heavily material of getting 10 microlitres is placed on metal aluminium foil electricity, and coupling mass spectrometer detects, and obtains the mass spectrogram of this beverage as shown in Figure 3; According to the high-resolution mass spectra peak (m/z) in mass spectrum, and can Direct Identification composition in the beverage of judgement mass spectra peak representative, data show that metallic aluminium foliation sheet can carry out quick direct-detection to different complex samples.
With reference to figure 4, utilize adopted metal aluminium foil electron spray ionisation device Direct Analysis the Terbinafine hydrochloride emulsifiable paste sample of thickness.Method is: the sample of getting the Terbinafine hydrochloride emulsifiable paste of 5 micrograms is directly placed in metal aluminium foil electrospray device, and the mass spectrometer that is coupled detects, obtain the mass spectrogram of this ointment as shown in Figure 4, the signal of protonated Terbinafine shows that metallic aluminium foliation sheet electrospray ionization mass spectrum system has detected the active ingredient Terbinafine of ointment.
For routine inspection, can take to use the mode of metal aluminium foil electrospray ionization mass spectrometry Direct Analysis, without sample pretreatment, consumption sample amount is few, easy and simple to handle, and quick obtaining detects spectrogram, and contrast with standard items spectrogram, can determinand corresponding to precise Identification, thus determined whether this composition, and this provides new solution by the rapid analysis method for complex sample.
the direct-detection of solid powder sample
The utility model is creative realization the mass spectral analysis of powder sample and detection also, and powder sample is placed in metal aluminium foil, adds reagent and extracts.For example, 5 milligrams of Ginseng Root Powder samples are placed on aluminium foil electron spray ionisation device, drip 10 microliter methanol solvents, utilize methyl alcohol to extract the composition in ginseng, connect high-tension electricity, extract produces electron spray ionisation, obtain the mass spectrogram of samples of Ginseng as shown in Figure 5, and confirmatory the repeatability of having assessed method, data show that tinsel electron spray ionisation device can load bearing solid sample, and can extract solid.Illustrate that the analysis of aluminium foil electrospray ionization mass spectrometry is successful for the analysis of powder sample.
albumen desalination is analyzed
The utility model also utilizes the hydrophobic characteristics of metal surface, can remove the salinity in protein solution containing.For example 10 microlitres being contained to the aqueous solution that lysozyme that concentration is 10 micromoles per liter and concentration is the sodium chloride salt of 2.5 mol/L is placed in aluminium foil, connect the high-tension electricity ionization of directly spraying, produce mass spectrogram as shown in Figure 6A, mass spectrogram has shown the signal of sodium chloride cluster ions, does not obtain the signal of lysozyme.In contrast, 10 microlitres are contained to the aqueous solution that lysozyme that concentration is 10 micromoles per liter and concentration is the sodium chloride salt of 2.5 mol/L to be placed on aluminum EFI ionization device, treat that salinity is at the dry and hard crystalline substance of air apoplexy, adopt pipettor to get 2 microlitre frozen water, after dissolving salinity and siphon away, albumen is because hydrophobic effect is still stayed metal surface, add again 10 microliter methanol/water/formic acid (1:1:0.1%, volume ratio) mixed solvent dissolves albumen the auxiliary generation spraying ionization of aluminum metal surface, obtains mass spectrogram as shown in Figure 6B.Except the signal of sodium chloride cluster ions, also obtained the multi-charge signal of lysozyme.Start tinsel desalination and carried out the new method of electron spray ionisation.
In addition, electron spray ionisation device of the present utility model is also applied to deactivators and the mass spectral analysis to the polypeptide containing activating agent.The gramicidins sample that for example 10 microlitres is contained to concentration and be 5 micromoles per liter is placed in aluminium foil, wherein to contain concentration be 5%(mass ratio) β – D-Glucose glycosides activating agent to sample, connect high-tension electricity and directly carry out electron spray ionisation, produce mass spectrogram as shown in Figure 9 A, mass spectrogram has shown the signal of glucoside cluster ions, does not obtain the signal of gramicidins.In contrast, above-mentioned sample is placed in aluminium foil, treat air-dry crystallization, adopt pipettor to get 2 microlitre frozen water, after lytic activity agent and siphon away, after continued operation three times, gramicidins is because hydrophobic effect is still stayed metal surface, the mixed solvent that adds again 10 microliter methanol/water/formic acid (1:1:0.1%, volume ratio) dissolves gramicidins the auxiliary generation spraying ionization of aluminum metal surface, obtains mass spectrogram as shown in Figure 9 B.Except the signal of glucoside cluster ions, also obtained the double charge signal of gramicidins.Started tinsel except activating agent and carried out the new method of electron spray ionisation.
thermal response detects
The utility model also utilizes metal thermal conductive resin and thermal endurance, can heat the sample carrying in metal aluminium foil, and detect thermal response product.For example, 10 microlitres are contained to the aqueous solution that myoglobins that concentration is 10 micromoles per liter and concentration is the ammonium acetate buffer solution of 20 micromoles per liter to be placed on aluminum EFI ionization device, when temperature is 20 ℃, connect the high-tension electricity ionization of directly spraying, produce mass spectrogram (as Fig. 7 A), acquisition valence state is+7 ,+8 and+9 myoglobins mass spectrogram, the molecule of the myoglobins calculating for 17566Da, shown that myoglobins has kept complete configuration.Adopt the bottom of electric iron heating aluminium foil, when sample temperature is 74 ℃, obtain the mass spectrogram (as Fig. 7 B) of myoglobins, obtain the myoglobins of 2 sequences, a lower valency signal that keeps the myoglobins (molecular weight is 17566Da) of complete configuration, another is apomyoglobin (molecular weight is 16951Da), produces the multi-charge mass spectrum of high valence state: the myoglobins mass spectrogram of+7 to+15 multi-charge, has shown the impact of aluminium foil heating on albumen configuration.Continue to be heated to 85 ℃, keep the lower valency signal of the myoglobins of complete configuration further to reduce, and the relative abundance of the multi-charge mass spectra peak of high valence state further raises, further shown the impact of rising temperature on the configuration of myoglobins.The above results shows, the electron spray ionisation device of metal aluminium foil can be successfully used to add thermal response.
More than experiment showed, that the utility model utilizes the electron spray ionisation device of tinsel, owing to using tinsel carrying sample, tinsel has good conductivity, surface hydrophobic, good thermal conductivity and thermal endurance.Tinsel electrospray ionization mass spectrometry has been widened the range of application of mass spectral analysis greatly.On the other hand, the utility model also can be used for the new function such as the extraction to sample, surperficial desalination, thermal analysis, realized pretreated analysis, break through technically conventional EFI ionization device and only can guide sample and ionization, the utility model both can carry out Direct Analysis to complex sample, was also suitable for, to carrying out Preprocessing, having developed new electrospray ionization mass spectrum technology, improve mass spectral analysis efficiency, expanded mass spectrometry applications field.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection range of the utility model claims.
Claims (8)
1. an electron spray ionisation device, is characterized in that, comprises voltage interface (1) and the tinsel (2) being connected with described voltage interface (1);
Described voltage interface (1) is for applying voltage to described tinsel (2);
Described tinsel (2) is for carrying testing sample (6) and utilizing described voltage to excite described testing sample (6) that spraying ion (8) occurs to ionize and produce.
2. electron spray ionisation device according to claim 1, is characterized in that, described tinsel (2) comprises the spout (4) that sprays described spraying ion (8) voltage interface for orientation.
3. electron spray ionisation device according to claim 2, it is characterized in that, described tinsel (2) comprises for carrying the main body of described testing sample (6), and two barrier plates that form an angle with described main body (3), described spout (4) is formed between described two barrier plates (3), and described two barrier plates (3) are 10～90 ° with the angle that described main body forms.
4. electron spray ionisation device according to claim 3, it is characterized in that, described main body is pentagon, comprise base, vertical with base two sides and respectively with the flanging of two side obliques, article two, flanging is vertically turned up to the same side and is formed described two barrier plates (3), offers breach to form described spout (4) between described two barrier plates (3).
5. electron spray ionisation device according to claim 1, is characterized in that, described electron spray ionisation device also comprises the support (9) for tinsel described in fixed support.
6. electron spray ionisation device according to claim 1, is characterized in that, described tinsel (2) thickness is 10～100 microns.
7. electron spray ionisation device according to claim 2, is characterized in that, the width of described spout (4) is 50～500 microns.
8. electron spray ionisation device according to claim 1, is characterized in that, described electron spray ionisation device also comprises the heater (7) being arranged near described tinsel (2) below, for described testing sample (6) is heated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320412840.XU CN203521365U (en) | 2013-07-11 | 2013-07-11 | Electrospray ionization device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320412840.XU CN203521365U (en) | 2013-07-11 | 2013-07-11 | Electrospray ionization device |
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| CN203521365U true CN203521365U (en) | 2014-04-02 |
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| CN201320412840.XU Withdrawn - After Issue CN203521365U (en) | 2013-07-11 | 2013-07-11 | Electrospray ionization device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104282524A (en) * | 2013-07-11 | 2015-01-14 | 香港理工大学 | Electrospray ionization device and method |
| CN110047731A (en) * | 2019-02-13 | 2019-07-23 | 中国科学院成都生物研究所 | A kind of electrospray device and method integrating microtrabeculae Yu microballoon |
-
2013
- 2013-07-11 CN CN201320412840.XU patent/CN203521365U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104282524A (en) * | 2013-07-11 | 2015-01-14 | 香港理工大学 | Electrospray ionization device and method |
| CN104282524B (en) * | 2013-07-11 | 2018-02-09 | 香港理工大学 | Electron spray ionisation device and method |
| CN110047731A (en) * | 2019-02-13 | 2019-07-23 | 中国科学院成都生物研究所 | A kind of electrospray device and method integrating microtrabeculae Yu microballoon |
| CN110047731B (en) * | 2019-02-13 | 2021-05-28 | 中国科学院成都生物研究所 | Electric spraying device and method integrating microcolumns and microspheres |
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