CN203393133U - Device for culturing anaerobic or facultative anaerobic microorganisms - Google Patents

Abstract

The utility model provides a cultivation device for anaerobic or facultative anaerobic microorganisms. The cultivation device comprises a roughly cup-shaped body, a cover and a one-way air outlet valve. The body has an inner cavity, and the top of the body is provided with a Opening, the cover is detachably connected to the top of the body and can seal the opening, the cover is provided with an air hole communicating with the inner cavity of the body, and the one-way air outlet valve is arranged on the air hole. The cultivating device used for cultivating anaerobic or facultative anaerobic microorganisms provided by the utility model has the characteristics of convenient use, low cost and simple operation.

Description

A cultivation device for anaerobic or facultative anaerobic microorganisms

technical field

The invention relates to a microorganism culture container, in particular to a culture bag used for culturing anaerobic or facultative anaerobic microorganisms.

Background technique

The biological characteristics of anaerobic bacteria and facultative anaerobic bacteria are anaerobic and microaerophilic. Currently, the commonly used technical means for the cultivation of anaerobic bacteria and facultative anaerobic bacteria include anaerobic tanks, anaerobic bags, and gloves. boxes, rotating tubes, and anaerobic maintenance systems. Theoretically, these methods mainly include: (1) cultivating by isolating it from the air or contacting the air as little as possible; (2) removing the air by filling it with carbon dioxide, hydrogen, nitrogen, argon, etc.; Deoxygenation methods, such as yellow phosphorus, metal chromium and dilute sulfuric acid, pyrogallic acid anaerobic culture method, etc.; (4) by adding reducing agents to the medium, such as 0.1% sodium thioglycolate, 0.01% Sodium sulfide, etc.; the market also includes some other reagents for anaerobic culture, such as the AnaeroPack, an anaerobic gas production bag invented by Mitsubishi Gas Chemical Co., Ltd. of Japan.

At present, in the field of microbial culture, the anaerobic culture devices used, such as anaerobic tanks, anaerobic gas-generating bags, chemical oxygen removal, etc., are generally more expensive, and require a higher degree of capital for the experimenter, and the test steps are also relatively expensive. cumbersome. Obviously, for the cultivation of some simple facultative anaerobic microorganisms, the cost of using anaerobic tanks or anaerobic gas bags is relatively high. In order to save money, some experimenters use food compression bags or clothing compression bags for facultative anaerobic microorganisms. The cultivation of aerobic microorganisms, but this kind of cultivation device obviously cannot meet the requirements of microbial cultivation, and is prone to air leakage.

Contents of the invention

In order to make up for the deficiencies in the prior art, the utility model provides a cultivation device for culturing anaerobic or facultative anaerobic microorganisms. Adopting the utility model for cultivating anaerobic or facultative anaerobic microorganisms has the advantages of convenient use, low cost, The characteristics of simple operation.

In order to achieve the purpose of this utility model, the adopted technical scheme is as follows: a culture device for anaerobic or facultative anaerobic microorganisms, the culture device includes a roughly cup-shaped body, a cover and a one-way outlet valve , the body has an inner cavity, the top of the body is provided with an opening, the cover is detachably connected to the top of the body and can seal the opening, the cover is provided with an air hole communicating with the inner cavity of the body, and the one-way air outlet valve is set on the stomata.

Threaded connection between the top of the body and the cover.

An elastic sealing ring is arranged between the upper surface of the top of the body and the lower surface of the top of the cover.

The middle part of the body is a soft transparent area of soft transparent material.

The soft transparent material is transparent soft plastic.

The one-way air outlet valve has a valve sleeve, a valve core and a valve seat. The bottom of the valve sleeve is fixed on the air hole of the cover, and the top of the valve sleeve protrudes outward. Both the bottom of the valve sleeve and the top of the valve sleeve are provided with through holes. It is located in the valve sleeve and the middle part of the valve seat forms a valve cavity that communicates with the bottom and top of the valve sleeve. The valve core is located in the valve cavity and can move back and forth in the valve cavity and can be sealed with the valve seat to close the valve. through holes on the bottom of the sleeve.

The valve core has a roughly hemispherical valve head, and the valve seat has a roughly hemispherical surface matching the valve head.

The valve seat is an elastic sealing ring.

The technical solution adopted by the utility model has the following beneficial effects: the middle part of the cultivation device body adopts transparent materials, which is easy to observe the cultivation status of the microorganisms cultured in the cultivation device; no other chemical substances need to be added, and other wastes will not be generated; After vacuuming, it is automatically sealed to form an anaerobic or micro-aerobic environment. It does not need to use other sealing devices such as water-stop clamps.

Description of drawings

Fig. 1 is a schematic structural diagram of a culture device provided by the present invention.

Fig. 2 is a schematic diagram of the use state of the cultivation device of the present invention.

Explanation of reference signs: 100 body; 101 inner cavity; 102 body top; 103 body bottom; 104 body middle; 200 cover; 201 elastic sealing ring; ; 304 valve core; 305 valve head; 306 valve seat; 400 petri dishes.

Detailed ways

Below in conjunction with accompanying drawing, the utility model is further described.

See Figures 1-2. The utility model provides a cultivation device for anaerobic or facultative anaerobic microorganisms. The cultivation device comprises a roughly cup-shaped body 100 , a cover 200 and a one-way air outlet valve 300 . The body has an inner cavity 101, and the top 102 of the body is provided with an opening. The cover 200 is detachably connected to the top 102 of the body and can seal the opening of the top of the body. Specifically, the cover and the top of the body are screwed together. The cover 200 is provided with an air hole 202 communicating with the inner cavity 101 of the main body. The one-way air outlet valve 300 is arranged on the air hole 202. The one-way air outlet valve can be connected with an external air pumping device, so that the gas in the culture device can be transferred from one-way The air outlet valve is drawn out, and the airtight environment in the culture device is ensured after vacuuming. In order to improve the sealing performance when the cover is connected to the top of the body, an elastic sealing ring 201 is provided between the upper surface of the top of the body and the lower surface of the top of the cover.

In order to facilitate the observation of the culture of the cultured microorganisms in the culture device, the middle part 104 of the body is a soft transparent area of soft transparent material, wherein the soft transparent material can be transparent soft plastic.

In the utility model, the one-way air outlet valve can adopt the existing one-way valve. As a preference, the one-way air outlet valve 300 has a valve sleeve 301, a valve core 304 and a valve seat 306, the bottom of the valve sleeve 301 is fixed on the air hole 202 of the cover, the top of the valve sleeve protrudes outward, the bottom of the valve sleeve and the valve sleeve The top is respectively provided with through holes 302, 303, the valve seat 306 is located in the valve sleeve 301 and the middle part of the valve seat forms a valve cavity connected with the bottom of the valve sleeve and the top through holes 302, 303, the valve core 304 is located in the valve cavity and It can move back and forth in the valve cavity and can seal fit with the valve seat 306 to close the through hole 302 at the bottom of the valve sleeve. The valve core preferably has a substantially hemispherical valve head 305 , and the valve seat 306 has a substantially hemispherical surface matching the valve head 305 . In order to improve the one-way pumping valve to better block the through hole at the bottom of the valve sleeve after pumping, the valve seat 306 adopts an elastic sealing ring.

The cover 200 of the culture device, the top 102 of the body and the bottom 103 of the body can be made of glass, hard plastic or hard metal materials (such as stainless steel, etc.).

When the cultivation device of the present utility model is in use, first open the cover, put the culture dish 400 into the inner cavity 101 of the main body, screw the cover 200 on the top 102 of the main body, and connect the suction pipe of the air pump or other simple air extraction devices to the The top of the one-way air outlet valve pumps air. Under the action of the external air extraction device, the air in the culture device is discharged out of the device through the one-way air outlet valve. When the external air extraction device is removed, a pressure difference is formed inside and outside the one-way air outlet valve. The valve core 304 is tightly attached to the valve seat 306, so that the entire culture device forms a closed anaerobic or microaerobic environment. Put the whole culture device into the incubator to cultivate anaerobic or facultative anaerobic microorganisms.

Claims (8)

1. A culture device for anaerobic or facultative anaerobic microorganisms, characterized in that the culture device comprises a roughly cup-shaped body, a cover and a one-way air outlet valve, the body has an inner cavity, and the body top An opening is provided, and the cover is detachably connected to the top of the body and can seal the opening. An air hole communicating with the inner cavity of the body is provided on the cover, and the one-way air outlet valve is arranged on the air hole. 2. The culture device according to claim 1, characterized in that the top of the body and the cover are screwed together. 3. The culture device according to claim 1 or 2, characterized in that an elastic sealing ring is provided between the upper surface of the top of the body and the lower surface of the top of the cover. 4. The culture device according to claim 1, wherein the middle part of the body is a soft transparent area made of soft transparent material. 5. The culture device according to claim 4, wherein the soft transparent material is transparent soft plastic. 6. The culture device according to claim 1, wherein the one-way air outlet valve has a valve sleeve, a valve core and a valve seat, the bottom of the valve sleeve is fixed on the air hole of the cover, and the top of the valve sleeve protrudes outwards , the bottom of the valve sleeve and the top of the valve sleeve are provided with through holes, the valve seat is set in the valve sleeve and the middle part of the valve seat forms a valve cavity that communicates with the bottom and top of the valve sleeve. The valve cavity moves back and forth and can be sealed with the valve seat to seal the through hole at the bottom of the valve sleeve. 7. The culture device according to claim 6, wherein the valve core has a substantially hemispherical valve head, and the valve seat has a substantially hemispherical surface matching the valve head. 8. The culture device according to claim 6 or 7, wherein the valve seat is an elastic sealing ring.

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