CN202705350U - Device for detecting migration movement and invasion movement of cells - Google Patents
Device for detecting migration movement and invasion movement of cells Download PDFInfo
- Publication number
- CN202705350U CN202705350U CN 201220360879 CN201220360879U CN202705350U CN 202705350 U CN202705350 U CN 202705350U CN 201220360879 CN201220360879 CN 201220360879 CN 201220360879 U CN201220360879 U CN 201220360879U CN 202705350 U CN202705350 U CN 202705350U
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- base plate
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- invasion
- cells
- migration
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Abstract
The utility model provides a device for detecting migration movement and invasion movement of cells. The device comprises a square bottom plate and a square plate which is formed by four side walls which are perpendicular to the bottom plate. At least two opposite side walls inside the square plate can be detached, and the upper surface of the bottom plate is provided with a cross coordinate reticle. Through a coordinate system on the bottom plate, the device for detecting the migration movement and the invasion movement of the cells can be used to directly observe the migration movement and the invasion movement of detected cells and can quantify the movement distance of the detected cells. The device is simple in operation, high in accuracy, easy to manufacture and low in cost. When the density of the cells is not high, direct counting to migration cells can be achieved, and the device has a very wide market prospect.
Description
Technical field
The utility model relates to a kind of cell migration and device of attacking motion of detecting.
Background technology
The method that detects at present cell migration in biology or medical experiment has cell cut (wound healing) method and Transwell method.The cell scratch method is fairly simple, only need to make a cut with liquid transfer gun head in the middle of the cell that covers with, and behind the cell that flush away is scratched, cultivates in 24 hours observation of cell and moves and get final product.The method is more coarse, can only be qualitative, can not be quantitative, and the cell of together growing for tight adhesion easily causes cell levitating in blocks and causes the shortcoming of the failure of an experiment, the method can not be used for observation of cell invasion and attack motion simultaneously.Transwell method equipment therefor is comprised of two cells up and down, it is the filter membrane of 3-8 μ m that there is an aperture centre, upper chamber adds the suspension of being examined cell, lower chamber adds the nutrient solution that often contains somatomedin or chemokine, putting 37 ℃ hatches a few hours, get at last filter membrane, through steps such as fixing, drying, dyeing, decolourings, the filter membrane after processing is put microscopically cell is counted.By the cell counting to filter membrane lower floor, reflect the transfer ability of cell.The shortcoming of the method mainly contains: device expensive, cell inoculation and detecting step are loaded down with trivial details, are difficult to carry out the migration of Real-Time Monitoring viable cell and move with invasion and attack.
The utility model content
The purpose of this utility model provides a kind of device that detects cell migration and invasion and attack motions, comprises a square floor and the square plate that forms perpendicular to four sidewalls of described base plate; It is detachable having two relative described sidewalls in the described square plate at least; Described base plate is provided with cruciform coordinate netting twine.This device can be placed in the conventional Tissue Culture Dish or culture plate, form an acellular space, when described dismountable sidewall is unloaded, cell in described Tissue Culture Dish or the culture plate can move to described baseplate zone, by described cruciform coordinate netting twine, but the migration of Real-Time Monitoring viable cell is mobile with invasion and attack.
In said apparatus, the vertical height of described sidewall should be less than the height of conventional Tissue Culture Dish or culture plate, specifically can be less than 15mm, such as 13mm; The length of side of described base plate can be between 5mm～30mm, so that described square plate is positioned over use in Tissue Culture Dish or the culture plate.
In said apparatus, described base plate is square or rectangular.
In said apparatus, it is that 20mm, width are the rectangle of 13mm that described base plate specifically can be length.
In said apparatus, described cruciform coordinate netting twine is by several rectilinear(-al)s parallel with described base plate four limits.
In said apparatus, for the ease of observation and differentiation, described cruciform coordinate netting twine becomes four lattice scale web areas with described flat plate separation; The length of side of described lattice specifically can be 0.02mm.
Said apparatus can be made by transparent material such as the plastics of routine.
Use the device of detection cell migration provided by the utility model and invasion and attack motions, advantage is: can be by the coordinate-system on the base plate, and direct viewing is examined migration and the invasion and attack motion of cell, and quantitative its move distance; Easy and simple to handle, accuracy is high, and makes simple, with low cost; When cell density is not high, can to the cell direct census of migration, have very wide market outlook.
Description of drawings
Fig. 1 is detection cell migration of the present utility model and the stereographic map of attacking the device that moves.
Fig. 2 is detection cell migration of the present utility model and the vertical view of attacking base plate in the device that moves.
Fig. 3 is positioned over sectional view in the Tissue Culture Dish for the device that the utility model is detected cell migration and invasion and attack motions.
Fig. 4 is for detecting the utility model the sectional view after cell migration and two sidewalls of the device of invasion and attack motion are removed.
Fig. 5 is the schematic diagram that cell moves at the utility model detection cell migration and the device base plate of attacking motion.
Among Fig. 1-5,1 is base plate, and 2 is sidewall, and 3 are the Tissue Culture Dish lid, and 4 is Tissue Culture Dish, and 5 is cell.
Embodiment
Below in conjunction with accompanying drawing the utility model is described further, but the utility model is not limited to following examples.
The device (Fig. 1) of the detection cell migration that the utility model provides and invasion and attack motions be one by rectangle base plate 1 and the square plate that forms perpendicular to four sidewalls 2 of base plate 1, wherein two relatively long sidewalls are detachable, and the sidewall that two other is relatively short and base plate 1 are one; Base plate 1 upper surface is provided with several rectilinear(-al) cruciform coordinate netting twines (Fig. 2) parallel with base plate four limits, base plate 1 is separated into four lattice scale web areas, separate with the cruciform white space between the lattice scale net, can be used for Real Time Observation and mensuration and examined cell to middle locomotory movement and Morphologic Parameters, the length of side of each lattice is 0.02mm.The vertical height of four sidewalls 2 is 13mm; The length of base plate 1 is 20mm, and width is 13mm.
In the device of above-mentioned detection cell migration and invasion and attack motions, it is detachable that four sidewalls 2 also can all be set as, and experimental particular case is observed after removing corresponding sidewall again; Base plate 1 also can be square; The vertical height of the length of base plate 1, width and sidewall 2 can be determined according to actual needs; Distance or lattice size between the straight line of cruciform coordinate netting twine also can be determined as required.
When using above-mentioned detection cell migration and attacking the device of motion, on base plate 1, can spread into fibronectin splicing variants (fibronectin), ln (laminin), collagen protein (collagen), the extracellular matrix glue such as vitronectin (vitronectin) or matrigel (matrigel) are used for observing and measure the invasion and attack campaign of being examined cell.When being used for the migration of observation adherent growth cell and attacking motion (Fig. 3), before the cell inoculation, the bottom surface of square plate base plate 1 is coated with some vacuum silicon rubbers (such as U.S. DOW CORNING company product etc.) gently, can be fixed on the bottom surface of Tissue Culture Dish or culture plate; Then in Tissue Culture Dish or culture plate, add single cell suspension, leave standstill and cultivated 4-6 hour; Remove two relatively long sidewall 2(Fig. 4), continue to cultivate 6-24 hour; Part is examined cell migration to the coordinate system zone (Fig. 5) of base plate 1, under the optics inverted microscope, observe and definite signaling distance and direction, and according to observation interval, calculate the cell migration movement rate, reflect migration and the invasive ability of cell with this.
Claims (9)
1. device that detects cell migration and invasion and attack motions comprises a square floor (1) and the square plate that forms perpendicular to four sidewalls (2) of described base plate; Have at least two relative described sidewalls (2) detachable in the described square plate; Described base plate (1) upper surface is provided with cruciform coordinate netting twine.
2. device according to claim 1, it is characterized in that: the vertical height of described sidewall (2) is less than 15mm.
3. device according to claim 2, it is characterized in that: the vertical height of described sidewall (2) is 13mm.
4. device according to claim 1 and 2, it is characterized in that: described base plate (1) is square or rectangular.
5. device according to claim 4, it is characterized in that: the length of side of described base plate (1) is 5mm～30mm.
6. device according to claim 5, it is characterized in that: described base plate (1) is that 20mm, width are the rectangle of 13mm for length.
7. device according to claim 1 and 2, it is characterized in that: described cruciform coordinate netting twine is by several rectilinear(-al)s parallel with described base plate (1) four limit.
8. device according to claim 7, it is characterized in that: described cruciform coordinate netting twine is separated into four lattice scale web areas with described base plate (1).
9. device according to claim 8, it is characterized in that: the length of side of described lattice is 0.02mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220360879 CN202705350U (en) | 2012-07-24 | 2012-07-24 | Device for detecting migration movement and invasion movement of cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220360879 CN202705350U (en) | 2012-07-24 | 2012-07-24 | Device for detecting migration movement and invasion movement of cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202705350U true CN202705350U (en) | 2013-01-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220360879 Expired - Fee Related CN202705350U (en) | 2012-07-24 | 2012-07-24 | Device for detecting migration movement and invasion movement of cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202705350U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111635858A (en) * | 2020-06-09 | 2020-09-08 | 中国医科大学附属第一医院 | Cell culture device and culture method |
-
2012
- 2012-07-24 CN CN 201220360879 patent/CN202705350U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111635858A (en) * | 2020-06-09 | 2020-09-08 | 中国医科大学附属第一医院 | Cell culture device and culture method |
| CN111635858B (en) * | 2020-06-09 | 2023-06-06 | 中国医科大学附属第一医院 | Cell culture device and cell culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 Termination date: 20140724 |
|
| EXPY | Termination of patent right or utility model |