CN202610234U - Negative pressure device for cell vaccination - Google Patents
Negative pressure device for cell vaccination Download PDFInfo
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- CN202610234U CN202610234U CN 201220254497 CN201220254497U CN202610234U CN 202610234 U CN202610234 U CN 202610234U CN 201220254497 CN201220254497 CN 201220254497 CN 201220254497 U CN201220254497 U CN 201220254497U CN 202610234 U CN202610234 U CN 202610234U
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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Abstract
本实用新型涉及一种用于细胞接种的负压装置。本实用新型所述的负压装置包括：负压发生器，用于在整个负压装置的管腔内产生细胞接种所需的负压；接种仓，用于装载接种细胞的组织工程支架以及细胞悬液；以及三通管，其主管连通负压发生器，支管连通所述接种仓，空余的管口封闭。本实用新型所述的用于细胞接种的负压装置设计新颖，接种操作简便，接种时间短，不会损伤接种细胞；对接种设备要求较低，制作成本低，易于普及应用；细胞的接种效率高，提高了细胞的有效使用率；可根据需要连接不同的接种仓以应对实际研究的需要，并且可以同时接种多个支架，提高研究效率，因而在组织工程的相关研究领域会具有较好的应用前景。
The utility model relates to a negative pressure device for cell inoculation. The negative pressure device described in the utility model includes: a negative pressure generator, which is used to generate the negative pressure required for cell inoculation in the lumen of the entire negative pressure device; an inoculation chamber, which is used to load tissue engineering scaffolds for inoculating cells and cell suspension; and a three-way pipe, the main pipe of which is connected to the negative pressure generator, the branch pipe is connected to the inoculation chamber, and the empty nozzle is closed. The negative pressure device for cell inoculation described in the utility model has a novel design, simple inoculation operation, short inoculation time, and no damage to inoculated cells; low requirements for inoculation equipment, low production cost, and easy popularization and application; cell inoculation efficiency High, improving the effective utilization rate of cells; different inoculation chambers can be connected according to needs to meet the needs of actual research, and multiple scaffolds can be inoculated at the same time to improve research efficiency, so it will have a good role in the related research fields of tissue engineering Application prospect.
Description
Technical field
The utility model relates to a kind of negative pressure device that is used for cell inoculation, is mainly used in the inside and outside structure engineering tissue of field of tissue engineering technology body.
Background technology
In the organizational project practice, often need be with seed cell in vitro culture or apply certain intervention, increase a certain amount of after, just can be inoculated into three-dimensional rack and get on.The high-level efficiency of cell inoculation and the cell uniform distribution on support, most important for the structure of engineering tissue.Research about the seed cell inoculation technique remains one of the important content in Tissue Engineering Study field.At present, the inoculation method of seed cell is divided into static inoculation method and dynamic inoculation method substantially.Static inoculation method is mainly methods such as sessile drop method, immersion method and microinjection, and above method is to make cell suspension get into internal stent through osmosis, and is easy and simple to handle, but it is lower to inoculate efficient, is difficult to realize the uniform distribution of cell on timbering material.Dynamically inoculation method mainly contains: methods such as centrifugal inoculation method, negative-pressure adsorption method, succusion, bio-reactor inoculation, not only improved the inoculation efficient of cell but also improved the spatial distribution of cell in support.But above-mentioned dynamic inoculation method all need design or buy expensive instrument and do and auxiliaryly could accomplish cell inoculation, has limited its popularization and application in the organizational project Related Research Domain to a certain extent.
For the negative-pressure adsorption method, vacuum pump suction and two kinds of PWR PLT of syringe pump are arranged at present, syringe negative-pressure adsorption method inoculating cell is existing report in many documents, and effect also obtains certainly.But, no matter be that support is put into syringe, closed injection device mouth then, negative pressure is made in pumpback; Still outside syringe, connect a container; Make the method for negative pressure with being placed on pumpback in the container; A syringe inoculation once can only be inoculated the cell of a support or the same individuality of a plurality of supports, is difficult to satisfy the research needs of large sample, and working efficiency is very low.Because in field of tissue engineering technology; No matter the structure of the engineering tissue research autogenous cells that use are static inoculation method or dynamic inoculation method more in the body, are mostly that placing support of a kind of cell in each inoculation storehouse inoculates; Traditional syringes negative-pressure adsorption method is satisfying the individual narrow spectrum while of above-mentioned inoculation; Need repeatedly the repeated inoculation operation, working efficiency is very low, and the operation of manpower pumpback syringe; All possibly there is subjective bias in each inoculation, the consistence of condition of negative pressure when being difficult to guarantee each individual inoculation.And, traditional syringes negative-pressure adsorption method inoculating cell, the negative pressure in the device is difficult to obtain quantize.Therefore press for this area work out a kind of new simple and easy to do, high efficiency, simultaneously can quantization device in the cell inoculation device of condition of negative pressure.
Summary of the invention
In order to overcome the some shortcomings of present syringe negative-pressure adsorption method on cell inoculation is used; The utility model provides a kind of novel negative pressure device that is used for cell inoculation; This device simple in structure, assemble easily and use; Can satisfy the cell inoculation requirement of inoculating Different Individual, a plurality of supports simultaneously, cell inoculation efficient is higher, simultaneously can the interior condition of negative pressure of quantization device.
The described negative pressure device that is used for cell inoculation of the utility model comprises: negative pressure generator is used in the tube chamber of whole negative pressure device, producing the required negative pressure of cell inoculation; Inoculate the storehouse, be used to load the tissue engineering bracket and the cell suspension of inoculating cell; And Y-tube, it is responsible for and is communicated with negative pressure generator, and arm is communicated with said inoculation storehouse, vacant mouth of pipe sealing.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model, the described negative pressure device that is used for cell inoculation also comprises vacuum meter, is connected between the person in charge of said negative pressure generator and said Y-tube.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model, the described negative pressure device that is used for cell inoculation comprises a plurality of Y-tubes, and the person in charge of these Y-tubes connects with series system, and the arm of each Y-tube all connects the inoculation storehouse; Said placed in-line Y-tube has two vacant mouths of pipe, and a mouth of pipe is communicated with said negative pressure generator, another mouth of pipe sealing.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model, the described negative pressure device that is used for cell inoculation also comprises vacuum meter, is connected between the person in charge of said negative pressure generator and said placed in-line Y-tube.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model, person in charge's mouth of pipe of the sealing of said Y-tube is with stopper or spiral cover sealing.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model; Said negative pressure generator is the syringe of big volume, through the pumpback piston then the process of release piston in the tube chamber of whole negative pressure device, produce the required negative pressure of cell inoculation.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model; Said inoculation storehouse be by the little centrifuge tube preparation of rubber stopper seal be suitable for inoculating small-sized inoculation storehouse less than the tissue engineering bracket of 7mm diameter, it inserts rubber plug through web member and is communicated with the arm of said Y-tube.Preferably, said web member is the thorn plug pin of vacuum blood taking needle.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model, said inoculation storehouse is the large-scale inoculation storehouse greater than the tissue engineering bracket of 7mm diameter that is suitable for by the arm that can directly be communicated with said Y-tube of 5ml or the preparation of 10ml syringe.
According to the described further characteristic that is used for the negative pressure device of cell inoculation of the utility model, pipe or the stopper that stops the piston pumpback is equipped with in the end in described large-scale inoculation storehouse.
The described negative pressure device that is used for cell inoculation of the utility model has following characteristics and advantage:
(1) for negative pressure was provided by vacuum pump, the negative pressure that the syringe pumpback is produced in the utility model also can satisfy the needs of cell inoculation, did not need main equipment, and is simple, is convenient to promote the use of;
(2) connected Y-tube cleverly as gas channel, a syringe can only be inoculated the perhaps deficiency of a support of a kind of cell when having improved the inoculation of conventional syringe negative pressure.The utility model can be inoculated the cell of a plurality of Different Individual simultaneously, and the support of multiple different size has improved inoculation efficient;
(3) adopt the utility model to inoculate simultaneously for a plurality of inoculations storehouse, each inoculation storehouse is in the same subnormal ambient, has reduced the manpower suction subjective bias that causes to a certain extent;
(4) be connected with vacuum meter in the syringe negative pressure system of the utility model, can quantize the inner vacuum magnitude of syringe negative pressure system.
In sum, the utility model is described, and to be used for the negative pressure classification inoculation apparatus of cell inoculation novel in design, inoculate easy and simple to handlely, and inoculation time is lacked, and can not damage inoculating cell; Lower to the inoculation equipment requirements, need not spend substantial contribution and buy special equipment, cost of manufacture is low, is easy to popularization and application; The inoculation efficient of cell is high, has improved the availability of cell; Can connect the needs of different inoculation storehouses as required with the reply practical study; And can inoculate a plurality of supports simultaneously; Improved postvaccinal support and be used to study and clinical working efficiency, thereby can have application promise in clinical practice in the Related Research Domain of organizational project.
Description of drawings
Fig. 1 is the described synoptic diagram that is used for the negative pressure device of cell inoculation of the utility model, and the negative pressure device shown in the figure has assembled 4 small-sized inoculation storehouses.
Fig. 2 is and the described large-scale inoculation storehouse that is used for the supporting design of negative pressure device of cell inoculation of the utility model, can be used for replacing the small-sized inoculation storehouse in the negative pressure device shown in Figure 1.
Among the figure: 1, negative pressure generator; 2, vacuum meter; 3, Y-tube; 4, small-sized inoculation storehouse; 5, pipe connecting; 6, closure; 7, rubber plug; 8, web member; 9, tissue engineering bracket; 10, large-scale inoculation storehouse; 11, the pipe or the stopper that stop the piston pumpback.
Embodiment
Be purpose, structure, characteristic and the function of further understanding the utility model, below provide to combine the detailed seeded process of embodiment to describe.
The described the most basic assembly that is used for the negative pressure device of cell inoculation of the utility model comprises: negative pressure generator 1, Y-tube 3 and inoculation storehouse.In order to measure and monitor the pressure in the negative pressure device, also increase a vacuum meter 2.
Fig. 1 has shown the described concrete synoptic diagram that is used for the negative pressure device of cell inoculation of the utility model.As shown in Figure 1; Described negative pressure device (for example adopts a big volume syringe; The 100ml syringe) as negative pressure generator 1; Be used in the tube chamber of whole negative pressure device producing the required negative pressure of cell inoculation, through the pumpback piston then the process of release piston in the tube chamber of whole negative pressure device, produce the required negative pressure of cell inoculation.This negative pressure device also comprises a vacuum meter 2, is used to measure and monitor the pressure in the negative pressure device.This negative pressure device comprises that also (this example is four Y-tubes to a plurality of Y-tubes 3; Also can connect more Y-tube according to actual needs); The person in charge's of first Y-tube a mouth of pipe is communicated with negative pressure generator 1 through pipe connecting 5, and another mouth of pipe connects the person in charge of second Y-tube, and the 3rd Y-tube successively is connected in series; Last Y-tube i.e. person in charge's of the 4th Y-tube the vacant mouth of pipe seals with closure 6 (for example, stopper or spiral cover).The arm of each Y-tube 3 is communicated with an inoculation storehouse respectively.In this example; The inoculation storehouse that is communicated with is small-sized inoculation storehouse 4; For example; By little centrifuge tube (for example, the eppendorf pipe that the laboratory the is commonly used) preparation of rubber plug 7 sealing be suitable for loading small-sized inoculation storehouse 4 less than the tissue engineering bracket 9 of 7mm diameter, it inserts rubber plugs 7 through web member 8 and is communicated with the arm of said Y-tube 3.Said web member 8 can adopt the thorn plug pin of vacuum blood taking needle, also can adopt the product of similar functions.
Fig. 2 has shown and the described large-scale inoculation storehouse 10 that is used for the supporting design of negative pressure device of cell inoculation of the utility model, can be used for replacing the small-sized inoculation storehouse 4 in the negative pressure device shown in Figure 1.For example, large-scale inoculation storehouse is the large-scale inoculation storehouse greater than the support of 7mm diameter that is suitable for by the arm that can directly be communicated with said Y-tube of 5ml or the preparation of 10ml syringe.As shown in Figure 2, pipe or the stopper 11 that stops the piston pumpback can be equipped with in the end in described large-scale inoculation storehouse.
Application examples: rabbit bone marrow mescenchymal stem cell inoculation
Before the described negative pressure device of use the utility model carries out the operation of negative pressure method inoculating cell; At first will carry out autoclaving with syringe, the rubber plug after the eppendorf pipe that cell suspension and tissue engineering bracket directly contact is perhaps reequiped; Other structures except that the negative pressure of vacuum table place in the Biohazard Safety Equipment classification inoculation apparatus after the sterilization subsequent use all through ethylene oxide sterilizing.Extract marrow 5ml from the new zealand white rabbit posterior superior iliac spine, use the adherent culture method to go down to posterity to be cultured to that to adjust concentration of cell suspension after the third generation be 1 * 10
6/ ml.Draw an amount of (confirming) cell suspension and will pass through the disinfectant support then and put into cell suspension, with the airtight inoculation of the rubber plug storehouse of inserting blood sampling acupuncture plug pin to inoculating in the storehouse according to the support volume; After at last each thorn plug pin and medical threeway being coupled together, each threeway is linked together, need keep and will tighten the sealing classification inoculation apparatus apart from the terminal spiral cover of vacuum table medical threeway farthest; Connect vacuum table and 100ml syringe and carry out cell inoculation, at the uniform velocity pumpback syringe piston to syringe tail end was kept about 10 seconds; Slowly discharge negative pressure to piston and be back to the syringe port place, repeat after about 10 times of above-mentioned " pumpback-releases " process support to be taken out from inoculating in the storehouse; Be placed in 24 orifice plates, shift out Biohazard Safety Equipment, in 37 ℃ of incubators, cultivated 3 hours; After treating that cell and support attach, support is moved in 6 orifice plates support in every hole; Perfect medium 6ml is added in every hole, continues to cultivate for use.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220254497 CN202610234U (en) | 2012-06-01 | 2012-06-01 | Negative pressure device for cell vaccination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220254497 CN202610234U (en) | 2012-06-01 | 2012-06-01 | Negative pressure device for cell vaccination |
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| Publication Number | Publication Date |
|---|---|
| CN202610234U true CN202610234U (en) | 2012-12-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220254497 Expired - Fee Related CN202610234U (en) | 2012-06-01 | 2012-06-01 | Negative pressure device for cell vaccination |
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| Country | Link |
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| CN (1) | CN202610234U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181668A (en) * | 2022-08-29 | 2022-10-14 | 上海大学 | A high-efficiency cell seeding device and method for tissue engineering scaffolds |
-
2012
- 2012-06-01 CN CN 201220254497 patent/CN202610234U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181668A (en) * | 2022-08-29 | 2022-10-14 | 上海大学 | A high-efficiency cell seeding device and method for tissue engineering scaffolds |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Jin Dan Inventor after: Xia Liheng Inventor before: Jin Dan Inventor before: Zhang Yongtao |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: JIN DAN ZHANG YONGTAO TO: JIN DAN XIA LIHENG |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121219 Termination date: 20150601 |
|
| EXPY | Termination of patent right or utility model |