A kind of stem cell extraction element
Technical field
The utility model relates to a kind of medical mechanism, particularly a kind of stem cell extraction element.
Background technology
Stem cell is meant the initiating cell with self and multidirectional differentiation potential, is the cells of origin of body, and can break up under given conditions becomes the cell that one or more constitute tissue or organ.Self is meant that stem cell can keep the size of stem cell colony through cell fission; Multidirectional differentiation is meant that stem cell has plasticity-; Under different internal milieus, can be divided into different histocyte (can be divided into neurocyte, scleroblast, myocardial cell etc. like mescenchymal stem cell) thereby the histoorgan of the various complicacies of formation body.(Mesenchymal Stem Cell MSC) is a kind of of stem cell to mescenchymal stem cell, gains the name because can be divided into stroma, has inferior all-round differentiation potential, under specific inside and outside environment, can induce differentiation to become multiple histocyte.Mescenchymal stem cell has the general character of stem cell, promptly has the biological characteristics of self and multidirectional differentiation potential; In addition, mescenchymal stem cell also has the characteristic of amplification in vitro.
After placenta mesenchyma stem cell was meant that the newborn infant is born, the stem cell that in placenta, exists wherein included with fetal tissue and joins the consistent stem cell of type, also contains the stem cell consistent with mother tissue matching.External evoked Study on Differentiation proves that placenta mesenchyma stem cell had both had the function (treatment disease in the blood system) that is divided into hemopoietic stem cell, has again to be divided into various histiocytic functions (being fit to histoorgan transplants and treatment).Because its source is easy and pure, regeneration power is strong,, immunogenicity more early stage than other adult mescenchymal stem cell tissue developments more a little less than, tissue is greatly compatible, the expense that obtains has become the main source of mescenchymal stem cell than plurality of advantages such as derived from bone marrow are low.
(placenta is a circle or oval-shaped plate-like organ, heavily about 500~600 grams, 18~20 centimetres of diameters, thick about 2.5 centimetres, thick middle, thin edge because the placenta volume is big.), extract the main mode of placenta mesenchyma stem cell at present, need be cut into 1cm to placenta with scissors earlier
3Following fritter, and then the further reaction digestion and the filtration etc. of milling, mainly there is following defective in the extraction element of existing placenta mesenchyma stem cell:
A. hand-manipulated loaded down with trivial details, manual work is relied on big;
B. length consuming time is unfavorable for that cell keeps active;
C. manual operation is unfavorable for stdn.
In sum, but study and develop a kind of mechanical automation and the controlled stem cell extraction element of standard becomes focus instantly.
The utility model content
The technical problem of the required solution of the utility model is to disclose a kind of stem cell extraction element; To overcome the existing artificial deficiency of extracting stem cell; Provide a kind of more robotization that is used for laboratory or medical institutions, cell preparation applicable industry circle, stdn, rapidly and efficiently, stem cell extraction element that use range is wider, make that laboratory, hospital or factory can safe mass-producing ground high-level efficiency and prepare stem cell in high quality.
More specifically; The purpose of the utility model is, adopts the modern mechanical automatic technology, the existing placenta cutting technique of improvement; A kind of placenta cutting machine of normal size and automation system device of subsequent reactions of cutting is provided, promptly a kind of automatically quick stem cell extraction element.This device can the whole placenta of disposable cutting, obtains to drop into behind the placenta fragment of identical size in the reaction tank to grind through filtering basin with the enzyme reaction solution reaction promptly get the placenta cells suspension after filtering i.e. stem cell suspension, formation one automatic stem cell extraction element.
A kind of stem cell extraction element that the utility model provides comprises placenta cutting unit, reaction tank, filtration unit and filtrate collection device, wherein:
Said placenta cutting unit comprises cutting part and interconnecting piece, and said cutting part is made up of cut part and base, and interconnecting piece is a lifting arm; Said interconnecting piece connects said base and said cut part; Described cut part is made up of horizontal cutter and the vertical cutter arranged in length and breadth; Described base is provided with the groove that the vertical and horizontal corresponding with said horizontal cutter and vertical cutter are arranged; Described interconnecting piece one end connects base, and the other end connects cutting part;
Said reaction tank links to each other with the placenta cutting unit, and the placenta fragment after the cutting directly gets into reaction tank and enzyme reaction solution reaction;
Said filtration unit links to each other with reaction tank;
Said filtrate collection device links to each other with filtration unit.
Preferably, said reaction tank is positioned at a side of placenta cutting unit, is provided with dog-house, liquid inlet and liquid outlet, and described dog-house and liquid outlet are positioned at the both sides of reaction tank, and liquid inlet is positioned at the upper surface of reaction tank.
Preferably, said reaction tank is provided with thermostat, CO
2Breather, automatic liquid feeder.
Preferably, the liquid outlet of said reaction tank is positioned at the top of said filtration unit.
Preferably, described filtration unit is positioned at the top of filtrate collection device.
Preferably, the cut part of the placenta cutting unit of said stem cell extraction element and the length of base are 20-30cm, and width is 20-30cm.
Preferably, the cut part of the placenta cutting unit of said stem cell extraction element is made up of box body, horizontal cutter and the vertical cutter that Open Side Down, is installed in the box body bottom at the bottom of the cutter of said horizontal cutter and vertical cutter.
Preferably, the horizontal cutter of placenta cutting unit of said stem cell extraction element and vertical cutter are to be movably arranged on said box body bottom.
Preferably, to surround length and width be the square of 0.2-1cm for per two vertical cuttves of the cut part of the placenta cutting unit of said stem cell extraction element and per two horizontal cuttves.
Preferably, the vertical cutter of the placenta cutting unit of said stem cell extraction element and horizontal cutter blade height are greater than 2.5cm.
Preferably, the vertical cutter of the placenta cutting unit of said stem cell extraction element, horizontal cutter and base adopt high temperature material.
Preferably, the blade thickness of the vertical cutter of the placenta cutting unit of said stem cell extraction element and horizontal cutter is 0.1-0.5mm.
Preferably, the blade of the vertical cutter of the placenta cutting unit of said stem cell extraction element and horizontal cutter adopts the stainless material that contains carbon, chromium, molybdenum to make.
Preferably, the base bottom of the placenta cutting unit of said stem cell extraction element is provided with a cooling thermostat.
Preferably, the constant temperature scope of the cooling thermostat of the base of the placenta cutting unit of said stem cell extraction element is: 0-6 ℃.
Preferably, the constant temperature scope of the cooling thermostat of the base of the placenta cutting unit of said stem cell extraction element is 4 ℃.
Preferably, the cutting part both sides of the placenta cutting unit of said stem cell extraction element can be furnished with handle, and it is flexible up and down to be used for the control linkage parts.
Preferably, the interconnecting piece of the placenta cutting unit of said stem cell extraction element is connected with an electric control gear, and the said interconnecting piece of electronic control is flexible up and down.
Preferably, the electric control gear of the placenta cutting unit of said stem cell extraction element is controlled by biswitch, and the distance between the biswitch is 20-100cm.
Preferably; The enzyme reaction solution that the reaction tank of described stem cell extraction element adds contains trypsin final concentration 0.125%), Dispase (Dispase II; Final concentration 0.2%) collagenase (final concentration 0.05%), DNase I (deoxyribonuclease I; Final concentration 0.2%), said enzyme reaction solution adds in the reaction tank through the liquid inlet of said reaction tank.
Preferably, the reaction tank reaction conditions of described stem cell extraction element is: the adding enzyme reaction solution stirred after the placenta fragment dropped into reaction tank, and reaction added calf serum after 15 minutes under 37 ℃ of following 5%CO2 conditions, in 30 seconds, stirred.
Preferably, the reaction tank of described stem cell extraction element is provided with reactive switches, and the reaction that is used to control reaction tank begins and finishes.
Preferably, the filtration unit of described stem cell extraction element is one 200 order metal filter screens.
Preferably, each sub-device of described stem cell extraction element all can be connected with computer and control.
The beneficial effect of the utility model is: after the utility model adopts said structure, according to the size and the thickness of placenta, can be disposable a whole placenta be cut into the fritter of fixed size, prepared fritter can directly be used for the extraction of stem cell; Because the material of blade adopts thin and sharp material, make and do not cause a large amount of cell injury in the cutting process because of reasons such as machinery, chemistry, temperature; Because the base of the cutting part of the said device of the utility model is furnished with the cooling thermostat, can guarantee that the cutting environment of placenta remains on 4 ℃ of activity with the stem cell of assurance gained; Described device of the utility model and the direct parts in contact of placenta are processed by high temperature material, the clean operation environment safety when having guaranteed to cut placenta but high-temperature sterilization is sterilized; The placenta fragment of cutting gained directly drops into reaction tank and enzyme reaction solution reaction, and reacted reaction solution can directly filter through filtration unit and be collected into the filtrate collection device, and can greatly reduce material and pollute, the manpower transport, and the reaction process stdn is controlled; The described device cutting efficiency of the utility model is high, easy to operate effectively to can greatly improve production efficiency, and its preparation technology is simple, and convenient quality control is more suitable for scale operation and commercial applications; Use range is wide especially, therefore applies easily, can have a tremendous social and economic benefits in the short period of time.
Description of drawings
Fig. 1 is the synoptic diagram of the utility model stem cell extraction element;
Fig. 2 is the diagrammatic cross-section of the placenta cutting unit of the utility model stem cell extraction element;
Fig. 3 is the cutting part cutting knife arrangement mode synoptic diagram of the placenta cutting unit of the stem cell extraction element among the utility model embodiment 1.
Embodiment
The utility model has been studied existing medical mechanical skill, and a kind of new stem cell extraction element and purposes are provided.
The utility model ultimate demand makes stem cell extraction element product and uses, and will enumerate embodiment below and further specify.If any problem, can contact directly with the contriver.Provide distribution and the position of the quantity of horizontal cutter and vertical cutter, the material of blade, horizontal cutter and vertical cutter, concrete shape and method of use and some experimental study contents of base groove by aforementioned summary of the invention in above-mentioned some experimental datas that provide and the following example; But should be appreciated that the utility model is not limited in listed research contents here; Should also be appreciated that term as used herein only is used to describe certain embodiments, and be not qualification the utility model.
Embodiment 1 stem cell extraction element structure and method of use thereof
The stem cell extraction element structure that is provided in order more clearly to describe the utility model below specifies conjunction with figs..Accompanying drawing 1 is the structural representation of the stem cell extraction element of the utility model.
A kind of stem cell extraction element that the utility model provides; Comprise placenta cutting unit (1), reaction tank (2), filtration unit (3) and filtrate collection device (4); Wherein: the placenta cutting unit comprises cutting part (5) and interconnecting piece (6); Cutting part is made up of cut part (7) and base (8), and interconnecting piece is a lifting arm; Interconnecting piece connects said base and said cut part; Cut part is made up of horizontal cutter (9) and the vertical cutter (10) arranged in length and breadth; Base is provided with the groove (11) that the vertical and horizontal corresponding with said horizontal cutter and vertical cutter are arranged; Interconnecting piece one end connects base, and the other end connects cutting part; Reaction tank links to each other with the placenta cutting unit; Placenta fragment after the cutting directly gets into reaction tank and enzyme reaction solution reaction; Reaction tank is positioned at a side of placenta cutting unit; Be provided with dog-house (12), liquid inlet (13) and liquid outlet (14), dog-house and liquid outlet are positioned at the both sides of reaction tank, and liquid inlet is positioned at the upper surface of reaction tank; Enzyme reaction solution contains trypsin final concentration 0.125%), Dispase (Dispase II, final concentration 0.2%) collagenase (final concentration 0.05%), in addition, reaction tank also is provided with thermostat, CO to DNase I (deoxyribonuclease I, final concentration 0.2%)
2Breather, automatic liquid feeder; Filtration unit links to each other with reaction tank, and the liquid outlet of reaction tank is positioned at the top of said filtration unit; Filtration unit is one 200 order metal filter screens (15); The filtrate collection device links to each other with filtration unit, and filtration unit is positioned at the top of filtrate collection device.
The method of use of the stem cell extraction element that the utility model provides is:
1. with all device sterilizations, sterilization in the system;
2. placenta is positioned on the base of placenta cutting part, opens electric-controlled switch placenta is cut into the fritter that even size is 1cm;
3. placenta fragment input reaction tank and enzyme reaction solution reaction stir, and reaction added calf serum after 15 minutes under 37 ℃ of following 5%C02 conditions, in 30 seconds, stirred;
4. after reaction finished, reaction solution flowed to metal filter screen from liquid outlet, is collected into the filtrate collection device after the filtration, promptly gets stem cell suspension-s.
Embodiment 2 stem cell extraction element placenta cutting unit structures
The placenta cutting unit of the stem cell extraction element that provides for more detailed explanation the utility model below specifies conjunction with figs..Accompanying drawing 2 is the diagrammatic cross-section of the placenta cutting unit of the utility model stem cell extraction element.
The placenta cutting unit of the stem cell extraction element that the utility model provides comprises cutting part and interconnecting piece (6), and wherein, cutting part is made up of cut part (7) and base (8), and interconnecting piece is a lifting arm; Interconnecting piece connects base and cut part; Cut part is made up of horizontal cutter (9) and the vertical cutter (10) arranged in length and breadth; Base is provided with the groove (11) that the vertical and horizontal corresponding with said horizontal cutter and vertical cutter are arranged; Interconnecting piece one end connects base, and the other end connects cutting part; The length of cut part and base is 25cm, and width is 25cm; Cut part is made up of box body, horizontal cutter (9) and the vertical cutter (10) that Open Side Down, is installed in the box body bottom at the bottom of the cutter of horizontal cutter and vertical cutter; Horizontal cutter and vertical cutter are to be movably arranged on the box body bottom, and each horizontal cutter or vertical cutter all are provided with a handle of a knife (16) that stretches out box body; It is the square of 5mm that per two vertical cuttves and per two horizontal cuttves surround length and width; Described vertical cutter and horizontal cutter blade height are 3cm; Vertical cutter, horizontal cutter and base adopt the high temperature resistant stainless steel material that contains carbon, chromium, molybdenum to make, and the thickness of blade is 0.2mm; The base bottom is provided with a cooling thermostat (17), can put into ice cube in this cooling thermostat, and keeping the temperature of base is 0~4 ℃; The cutting part both sides can be furnished with handle, and it is flexible up and down to be used for the control linkage parts; In addition, the interconnecting piece of this device also can be connected with an electric control gear, and electric control gear is controlled by biswitch, and between the biswitch apart from 30cm.The said interconnecting piece of electronic control is flexible up and down, realizes the automated operation of this device.
The device that the utility model provides is when operation; Can be with the parts that directly contact in placenta such as cut part, base etc. through the high-temperature sterilization sterilization; Then placenta directly is placed on the base, manually or automatically cut part is moved downward through the flexible up and down of interconnecting piece, make horizontal cutter and vertical cutter insert on the base with its corresponding groove in; This moment, placenta can be used for the operation of follow-up extraction stem cell by the disposable fritter that cuts into identical size.Placenta fragment after cutting is accomplished can directly add in the reaction tank through dog-house and reacts; Wherein enzyme reaction solution can be selected for use and contain trypsin final concentration 0.125%), Dispase (Dispase II; Final concentration 0.2%) collagenase (final concentration 0.05%), DNase I (deoxyribonuclease I; Final concentration 0.2%) reaction solution adds reaction tank through liquid inlet, after stirring, and 37 ℃ of following 5%CO
2Reaction added calf serum after 15 minutes under the condition, in 30 seconds, stirred, and the liquid outlet through reaction tank grinds through the filter screen of filter device and filters back inflow filtrate collection device and promptly get placenta stem-cell suspension-s.
The above-described preferred embodiment of utilizing specifies the utility model, and the scope of unrestricted the utility model.Those skilled in the art can be through after reading the utility model; Make trickle change box adjustment; For example: adjust distribution and position, the enzyme reaction solution of the quantity of horizontal cutter and vertical cutter, the material of blade, horizontal cutter and vertical cutter component and corresponding content, connect the pipeline material between each parts, the reaction times of reaction tank, material of filtering net or the like; Will the can yet be regarded as main idea place of the utility model does not also break away from the spirit and the scope of the utility model.
In sum, the utility model provides a kind of design of new stem cell extraction element, according to the size and the thickness of placenta, can be disposable a whole placenta be cut into the fritter of fixed size, and prepared fritter can directly be used for the extraction of stem cell; Because the material of blade adopts thin and sharp material, make and do not cause a large amount of cell injury in the cutting process because of reasons such as machinery, chemistry, temperature; Because the base of the cutting part of the said device of the utility model is furnished with the cooling thermostat, can guarantee that the cutting environment of placenta remains on 4 ℃ of activity with the stem cell of assurance gained; Described device of the utility model and the direct parts in contact of placenta are processed by high temperature material, the clean operation environment safety when having guaranteed to cut placenta but high-temperature sterilization is sterilized; The placenta fragment of cutting gained directly drops into reaction tank and enzyme reaction solution reaction, and reacted reaction solution can directly filter through filtration unit and be collected into the filtrate collection device, and can greatly reduce material and pollute, the manpower transport, and the reaction process stdn is controlled; The described device cutting efficiency of the utility model is high, easy to operate effectively to can greatly improve production efficiency, and its preparation technology is simple, and convenient quality control is more suitable for scale operation and commercial applications; Use range is wide especially, therefore applies easily, can have a tremendous social and economic benefits in the short period of time.