CN202063923U - Feedback pneumatic control compressive stress cell culture appliance - Google Patents
Feedback pneumatic control compressive stress cell culture appliance Download PDFInfo
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- CN202063923U CN202063923U CN 201120195323 CN201120195323U CN202063923U CN 202063923 U CN202063923 U CN 202063923U CN 201120195323 CN201120195323 CN 201120195323 CN 201120195323 U CN201120195323 U CN 201120195323U CN 202063923 U CN202063923 U CN 202063923U
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- cell culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The utility model relates to a feedback pneumatic control compressive stress cell culture appliance. According to the device, a high-pressure gas storage tank is connected to a three-way pipe a through a switch, a reducing valve and a linkage guide pipe, is connected with a micro booster pump through a lower linkage guide pipe, a linkage guide pipe and an injector, is connected to a three-way pipe b through the linkage guide pipe, and is communicated with a needle head through a connecting guide pipe, a pressure gauge, the upper end of a bacterial filter and the lower end of the bacterial filter; a rubber plug is arranged on a measuring cylinder glass container; an upper outer reinforcing plate is arranged on the rubber plug; a lower outer reinforcing plate is arranged below a tubular glass container; the needle head passes through the rubber plug and enters the tubular glass container; and a cell culture dish is arranged at the bottom of the measuring cylinder glass container. The feedback pneumatic control compressive stress cell culture appliance overcomes the defects that a closed container is not easy to open or close and cannot bear a certain stress. In the appliance, the micro booster pump is ingeniously integrated with the compressive stress cell culture appliance, and the compressive stress cell culture appliance which has a simple structure, is convenient to operate and has reliable effect is provided for biological scientific research.
Description
Technical field
The utility model relates to a kind of biological research equipment, particularly a kind of reaction type gas control stress cell culture apparatus.
Background technology
Before the present utility model, the stress cell culture apparatus that uses in the present biological scientific research all compares expensive, neither be very good to the control of persistence stress.The influence of mechanical stress cell growth also is the difficult point that always exists.Therefore, this research can't be carried out in a lot of laboratories, or it is too high to buy a cost, or the persistence stress is undesirable, causes the effect out of true, influences experimental data.
For not only easily opening and closing but also can bear the encloses container of a certain size stress (particularly malleation), effect is all undesirable at present.Present device has inlet mouth and air outlet more, and the two has all installed valve, has increased the loaded down with trivial details property of device.
Summary of the invention
The purpose of this utility model just is to overcome above-mentioned defective, develops a kind of reaction type gas control stress cell culture apparatus.
The technical solution of the utility model is:
A kind of reaction type gas control stress cell culture apparatus, its major technique is characterised in that high pressure tank through switch, reducing valve, and the link conduit is communicated with Y-tube a, and Y-tube a is communicated with link conduit, link conduit down again respectively; Following link conduit connects micro-force (forcing) pump by syringe, and the link conduit connects Y-tube b, and Y-tube b distinguishes linking conduit, tensimeter and sterilization filter upper end again, and the sterilization filter lower end is communicated with syringe needle; Rubber plug is to go up the external reinforcement plate on the rubber plug on the cylinder type Glass Containers, and following external reinforcement plate is below the cartridge type Glass Containers, and syringe needle penetrates in the rubber plug inlet cartridge type Glass Containers, and Tissue Culture Dish is placed in cylinder type Glass Containers bottom.
Reaction type gas control stress cell culture apparatus, its major technique are characterised in that by micro-force (forcing) pump analog feedback Controlling System, monitor and keep the required setting pressure of experiment in real time.
The beneficial effects of the utility model are, with micro-force (forcing) pump and the ingenious combination of stress cell culture apparatus, for biological scientific research provides a stress cell culture apparatus simple in structure, easy to operate, reliable for effect, greatly improved control, monitored and keep, and then improved the science and the accuracy of experiment stress.Y-tube not only can linking conduit conducting gas, can also change the direction of gas flow by the valve of swivel tee pipe dexterously, had both played the effect of switch, plays the effect of link again, and simplified device.
Other advantages of the present utility model and effect will go on to say below.
Description of drawings
Fig. 1--the utility model structural principle synoptic diagram.
The concrete dynamic indicator card of using of Fig. 2--the utility model.
Each label represents that each parts is as follows among the figure:
High pressure tank 1, switch 2, reducing valve 3, link conduit 4, Y-tube a5, micro-force (forcing) pump 6, syringe 7, down link conduit 8, link conduit 9, tensimeter 10, link conduit 11, Y-tube b12, sterilization filter 13, syringe needle 14, screw 15, go up cell 21 in external reinforcement plate 16, rubber plug 17, cylinder type Glass Containers 18, steel nail 19, Tissue Culture Dish 20, the Tissue Culture Dish, external reinforcement plate 22 down.
Embodiment
As shown in Figure 1 and Figure 2:
High pressure tank 1 is communicated to Y-tube a5 through the switch 2 on it and behind reducing valve 3 by link conduit 4, and Y-tube a5 second end, the 3rd end are communicated with link conduit 8, link conduit 9 down respectively; Link conduit 8 is connected with micro-force (forcing) pump 6 by syringe 7, and link conduit 9 is communicated with Y-tube b12, and Y-tube b12 second end is communicated with tensimeter 10 by linking conduit 11, and Y-tube b12 the 3rd end is communicated with the upper end of sterilization filter 13; The lower end of sterilization filter 13 is communicated with syringe needle 14; On cylinder type Glass Containers 18, add a rubber plug 17, form an encloses container; Last external reinforcement plate 16, following external reinforcement plate 22 are closely oppressed on rubber plug 17 and below the cylinder type Glass Containers 18 by quadruplet screw 15, steel nail 19 respectively; Syringe needle 14 penetrates in the rubber plug 17 inlet cartridge type Glass Containerss 18, and Tissue Culture Dish 20 is placed in the bottom of cylinder type Glass Containers 18, is the cultured cells of wanting 21 in it.
The utility model application process is described as follows:
What store in the high pressure tank 1 is to contain 5%CO2 and 95% air compressed mixed gas, the gas concentration in the simulation CO2 cell culture incubator; Shut immediately after switch 2 is opened, just can make and store a certain amount of mixed gas between switch 2 and the reducing valve 3; By reducing valve 3, linking conduit 4, Y-tube a5, linking conduit 9, Y-tube b12, sterilization filter 13, syringe needle 14, high pressure tank 1 adds gas carrier in the encloses container of cylinder type Glass Containers 18 and rubber plug 17 compositions, by the pressure in the tensimeter 10 detection encloses containers, after reaching the required pressure of experiment, regulating Y-tube is that (Fig. 2: A is to B for Y-tube a5, promptly be rotated counterclockwise 90 ° of angles), change the gas flow direction, cell stress loaded.Because device has not only been simplified in the use of Y-tube, has exempted the use of inlet mouth, air outlet and valve, and the flow direction of pilot-gas effectively, and then controlled effectively and tested required stress.
By micro-force (forcing) pump 6, realize feedback control to whole device stress.Set a stress before using earlier micro-force (forcing) pump 6 in, its size should be the indicated stress sizableness of tensimeter 10 with testing required stress, configures the flow velocity of gas simultaneously.When the indicated stress sizableness of the setting stress of micro-force (forcing) pump 6 and tensimeter 10, trace pressurization air pump inoperative; In case the stress size in the encloses container (tensimeter 10 indicated stress) is less than the required stress of experiment, promptly for micro-force (forcing) pump, true pressure is during less than setting pressure, then micro-force (forcing) pump 6 just can be with the mixed gas in certain flow velocity propelling syringe 7, up to reaching micro-force (forcing) pump 6 setting pressure in advance.So just, realized the real-time monitoring of testing required stress and continue to keep, experiment condition is more rigorous, science and controlled thereby make, compare with present existing stress cell culture apparatus, the use of trace force (forcing) pump is like being a feedback control system, it is not only detecting the stress of the process of experiment in real time, in a single day and find that stress has descended, also can in time replenish mixed gas, up to reaching and keeping and test required stress.
Last external reinforcement plate 16, following external reinforcement plate 22 and quadruplet screw 15, steel nail 19 can make rubber plug 17 and cylinder type Glass Containers 18 seal fully well, form an encloses container, and can bear certain stress, keep can not leaking gas for a long time.Experiment shows because the fixed action of external reinforcement plate, can make experimental pressure when 100kPa continues 24h fall less than 1%.Syringe needle 14 penetrates rubber plug 17, realizes the loading of stress by this syringe needle 14.
Objectionable impuritiess such as connecing sterilization filter 13 above the syringe needle 14 can the airborne impurity of filtering, bacterium meet Y-tube b12 above the sterilization filter 13, turnover direction that can adjustments of gas.Sterilization filter 13 contains the filter membrane that diameter is 0.22um, can remove the deleterious material of growth of the pair cells such as impurity, bacterium, mycoplasma of mixed gas in the high pressure tank.Reducing valve 3 is regulated the airflow of mixed gas amount, allows gas slowly flow into link and leads 4 pipes, and the pressure of whole device is slowly raise, and improves the security and the controllability of device; Pressure in the trace force (forcing) pump 6 monitoring encloses containers, micro-force (forcing) pump 6 volumes are little, can put into the CO2 cell culture incubator, and the battery of substituting the bad for the good before the use in the CO2 cell culture incubator, need not to connect power supply again;
The pressure-loaded process explanation:
After opening the switch 2 of high pressure tank 1, shut rapidly, just gathered a certain amount of mixed gas between reducing valve 3 and the switch 2 like this, regulate the fine setting of reducing valve 3 then, make mixed gas slowly flow into linking conduit 4, in Y-tube a5, linking conduit 9, Y-tube b12, sterilization filter 13, syringe needle 14 inlet cartridge type Glass Containerss 18, as shown 1; By the pressure in the 10 detection limit cartridge type Glass Containerss 18 of the tensimeter above the Y-tube b12, after reaching setting pressure, swivel tee pipe a5, entering of blocking-up mixed gas, as shown 2, just can realize storing a certain amount of compressed mixed gas in the cylinder type Glass Containers 18, thereby realize certain stress, 20 inner cells 21 of the Tissue Culture Dish in the cylinder type Glass Containers 18 are pressurizeed.
The pressure monitoring process explanation:
Under the situation of Fig. 2, by Y-tube a5, mixed gas changes direction, and (A is to B, promptly be rotated counterclockwise 90 ° of angles) flow in the syringe 7 of micro-force (forcing) pump 6, reach a constant volume after, just stored a certain amount of mixed gas in the syringe 7, (B is to C for swivel tee pipe a5 again, promptly along or be rotated counterclockwise 180 ° of angles), the air flow line that makes micro-force (forcing) pump 6 is realized the testing process of stress in cylinder type Glass Containers 18.
Set the pressure in the micro-force (forcing) pump 6, the pressure required with experiment equates.In case the stress size in the Tissue Culture Dish 20 is less than setting pressure, promptly for micro-force (forcing) pump, true pressure is during less than setting pressure, then by micro-force (forcing) pump 6 pressurizations, reach set the stress size after, stop pressurization, so just realized the real-time monitoring of stress and continue to keep.
The utility model is by micro-force (forcing) pump analog feedback Controlling System, not only detecting the stress of the process of experiment in real time, and in case find that stress has descended, also can in time replenish mixed gas, up to reaching and keeping the required stress of experiment, promptly monitor and keep the required setting pressure of experiment in real time.
Claims (2)
1. a reaction type gas control stress cell culture apparatus is characterized in that high pressure tank is communicated with Y-tube a through switch, reducing valve, link conduit, and Y-tube a is communicated with link conduit, link conduit down again respectively; Following link conduit connects micro-force (forcing) pump by syringe, and the link conduit connects Y-tube b, and Y-tube b distinguishes linking conduit, tensimeter and sterilization filter upper end again, and the sterilization filter lower end is communicated with syringe needle; Rubber plug is to go up the external reinforcement plate on the rubber plug on the cylinder type Glass Containers, and following external reinforcement plate is below the cartridge type Glass Containers, and syringe needle penetrates in the rubber plug inlet cartridge type Glass Containers, and Tissue Culture Dish is placed in cylinder type Glass Containers bottom.
2. a kind of reaction type gas control stress cell culture apparatus according to claim 1 is characterized in that external reinforcement plate, following external reinforcement plate pass through screw respectively, steel nail is oppressed on rubber plug and below the cylinder type Glass Containers, forms an enclosed space.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120195323 CN202063923U (en) | 2011-06-08 | 2011-06-08 | Feedback pneumatic control compressive stress cell culture appliance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120195323 CN202063923U (en) | 2011-06-08 | 2011-06-08 | Feedback pneumatic control compressive stress cell culture appliance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202063923U true CN202063923U (en) | 2011-12-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120195323 Expired - Fee Related CN202063923U (en) | 2011-06-08 | 2011-06-08 | Feedback pneumatic control compressive stress cell culture appliance |
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| Country | Link |
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| CN (1) | CN202063923U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229888A (en) * | 2011-06-08 | 2011-11-02 | 南京大学医学院附属鼓楼医院 | Feedback-type pneumatic-control pressure stress cell culture device |
-
2011
- 2011-06-08 CN CN 201120195323 patent/CN202063923U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229888A (en) * | 2011-06-08 | 2011-11-02 | 南京大学医学院附属鼓楼医院 | Feedback-type pneumatic-control pressure stress cell culture device |
| CN102229888B (en) * | 2011-06-08 | 2013-06-12 | 南京大学医学院附属鼓楼医院 | Feedback-type pneumatic-control pressure stress cell culture device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111207 Termination date: 20140608 |