CN201724904U - Vitamin fluorescence detection system - Google Patents
Vitamin fluorescence detection system Download PDFInfo
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- CN201724904U CN201724904U CN2010200432568U CN201020043256U CN201724904U CN 201724904 U CN201724904 U CN 201724904U CN 2010200432568 U CN2010200432568 U CN 2010200432568U CN 201020043256 U CN201020043256 U CN 201020043256U CN 201724904 U CN201724904 U CN 201724904U
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Abstract
The utility model belongs to the technical field of biological detection, and relates to a vitamin fluorescence detection system, comprising a light source, a micropore plate carried with a reagent, a signal acquisition processing circuit and a microprocessor, wherein the micropore is placed on a scanning device; the scanning device is controlled by the microprocessor and can move along the horizontal and vertical directions; the light source is a halogen lamp, and the light which is generated by the halogen lamp generates into ultraviolet exciting light by a narrowband light filter with the wavelength of 345nm; the signal acquisition device comprises a narrowband light filter, optical fiber, a condensing lens, a fluorescence filter and a photoelectric multiplier tube; and the signal processing circuit is used for converting fluorescence signals detected by the photoelectric multiplier tube into electric signals, processing the electric signals and sending the processed electric signals into the microprocessor. The vitamin fluorescence detection system has the advantages of simple design, low price, and accurate and fast detection.
Description
Technical field
The utility model belongs to technical field of biological, relates to a kind of fluorescence harvester that is used for the vitamin fluoroscopic examination.
Background technology
Nutrition is one of sign of a national economic development.Since China's reform and opening-up, very big variation has taken place in economy.Along with expanding economy, and the progress of hygiene industry, all obtain in various degree correction in the low albumen disease of China, iron deficiency disease and calcium deficiency disease.But nutritive disease is still perplexing China people.Chinese residents nutrition in 2002 and investigation of health conditions show, China resident's vitamin A, B
1, B
2, the C insufficiency of intake, upgrowth and development of children dead-band 14.3%, 3-12 year vitamin A edge shortage rate are 45.1%.
Wherein especially VAD is one of at present main in the world deficiency disease, is listed in the children's health problem that emphasis solves by world's children's summit meeting.Vitamin A has the normal growth of keeping, reproduction, vision and anti-infectious function, can reduce children's anti-infective function during famine, causes the incidence of childrens respiratory tract and infection of digestive canal disease to rise.This two classes disease is the one of the main reasons that threatens Chinese children health at present; The shortage of folic acid can cause the generation of baby's neural tube defects, has directly influenced the quality of newborn population.
Though a variety of vitamin detection techniques are arranged at present, but because checkout equipment, detection method, many effects limit such as sample size that detection needs and accuracy, can't adapt to wide clinical application, seriously hinder China's hypovitaminosis evaluation and intervened extensively carrying out of work.
The utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, provides a kind of fluorescence harvester that is applicable to that the vitamin fluoroscopic examination is used, to satisfy the needs of clinical practice.
For this reason, the utility model adopts following technical scheme.
A kind of vitamin fluorescence detecting system comprises light source, is loaded with microwell plate, signal pickup assembly, signal processing circuit and the microprocessor of reagent, and described microwell plate is placed on the scanister; Described scanister is subjected to the control of microprocessor, can be along level and movement in vertical direction; Described light source is a Halogen lamp LED, and the light of its generation generates uv excitation light by wavelength for the 345nm narrow band pass filter; Described signal pickup assembly comprises narrow band pass filter, optical fiber, collector lens, fluorescence filter sheet and photomultiplier, described optical fiber is Y type conduction optical fiber, its public port is near described microwell plate, a bifurcated port links to each other with described light source, uv excitation light is radiated on the reagent that places microwell plate after conducting Optical Fiber Transmission and collector lens through the Y type, the fluorescence of the 485nm wavelength that is excited by reagent place goes out from another divergent ends oral instructions of Y type conduction optical fiber, and after the fluorescence filter sheet filters, be admitted to photomultiplier; Described signal processing circuit is used for converting the fluorescence signal that described photomultiplier detects to electric signal, and to sending into microprocessor after this electric signal processing.
As preferred implementation, described Y type conduction optical fiber is quartz glass optical fiber, and the branch road of the ultraviolet light of its transmission light source emission is a silica fibre, is glass optical fiber and fluorescence signal is transmitted branch road.Described signal processing apparatus comprises anti-phase treatment circuit, screens amplification treatment circuit and microprocessor and A/D change-over circuit, is admitted to the examination amplification treatment circuit behind the anti-phase treatment circuit of electric signal process by described photomultiplier output; Described examination amplification treatment circuit comprises threshold circuit, on-off circuit, 10 times of amplifying circuits, 1 times of amplifying circuit and 1/10 times of amplifying circuit, described threshold circuit is by two voltage comparators, produce 9V and two threshold voltage signals of 0.9V, these two signals are admitted to microprocessor and on-off circuit simultaneously respectively, by on-off circuit according to a circuit in 10 times of amplifying circuits of two threshold voltage signal gatings, 1 times of amplifying circuit and the 1/10 times of amplifying circuit; Be admitted to microprocessor behind the output process A/D change-over circuit of described examination amplification treatment circuit.
The utlity model has following technique effect: (1) simplicity of design, cheap, detect accurately with quick; (2) the Y type quartz glass optical fiber that adopts of native system, what it adopted ultraviolet signal transmission branch road is silica fibre, and what fluorescence signal transmission branch road was adopted is glass optical fiber.This optical fiber structure has just met the designing requirement of native system.Because for the less ultraviolet light of wavelength, frequency is higher, its to the requirement of transmission medium also than higher, so adopt silica fibre to transmit, and for the long fluorescence of wavelength, its frequency is lower, so to the requirement of transmission medium also than higher, so adopt glass optical fiber to transmit.(3) the utility model is according to the size of the electric signal of photomultiplier output, designed a kind of signal screening amplification treatment circuit, make signal when entering A/D converter, meet the area requirement of its analog input, and meet the accuracy requirement that the vitamin fluorescence signal detects.
Description of drawings
Fig. 1 vitamin fluorescence detecting system principle.
Fig. 2 fluorescence signal collection of the present utility model and detection schematic diagram.
Fig. 3 signal processing circuit structure block diagram of the present utility model.
Embodiment
Below in conjunction with drawings and Examples vitamin fluorescence detecting system of the present utility model is described further.
Whole vitamin fluorescence detecting system principle of work as shown in Figure 1.Comprise light source, be loaded with microwell plate, scanister, signal pickup assembly, signal processing circuit and the microprocessor of reagent.
As light source, Halogen lamp LED is the uv excitation light that 345nm narrow band pass filter 6 generates by wavelength, through fiber-optic illuminated on the reagent for preparing with Halogen lamp LED.Owing to contain the fluorescent material of variable concentrations in the special reagent, so can inspire the fluorescence of varying strength, this fluorescence is delivered to photomultiplier through Optical Fiber Transmission, is admitted in the signal processing circuit by the electric signal of photomultiplier collection, carries out further processing and amplifying.
In this system, light source is fixed, realizes scanning so need the reality of sample to move.The utility model has designed mechanical scanner, and its main effect is to deposit the microwell plate that sample reagent is housed, and make the microwell plate that is placed on the carriage under the drive of stepper motor along level and movement in vertical direction.The realization of scanning process depends on the design and the control of scanister.The core component of scanister is 2 phase reaction formula stepper motors, and the scanning framework is fixed on by belt on the axle of stepper motor, and its operation is driven by the operation of stepper motor.On the other hand, the detected signal of photomultiplier is admitted in the microprocessor through after certain made of hardware circuits which process, is further processed and analyzes.Final result is exported by microprocessor.
As the control core of whole vitamin fluorescence detecting system, the effect of microprocessor is very important.It is controlling the operation of light source, various function realization parts; Realize the control detection of various kinds of sensors; Carry out signal Processing; And, drive scanning mechanism, thereby the scan task of finishing along level and vertical direction operation by driving and control to stepper motor.
The harvester of the utility model fluorescence mainly comprises light source 1, narrow band pass filter 6, optical fiber 2, collector lens 3, fluorescence filter sheet 4 and photomultiplier 5.Light source 1 produces luminous energy, is exactly information carrier; Photomultiplier 5 detects luminous energy, and it is for conversion into the form of electricity; Optical fiber 2 is used for conducting luminous energy.Concrete fluorescence signal collection and detection principle such as Fig. 2.
It is that Halogen lamp LED is the uv excitation light that 345nm narrow band pass filter 6 generates by wavelength that light source 1 adopts light source, by being radiated on the sample of microwell plate behind its ultraviolet light that emits process optical fiber 2 transmission and the collector lens 3.Contain vitamin sample in the sample through extraction, so, under the irradiation of ultraviolet light, can inspire wavelength and be the fluorescence about 485nm, fluorescence signal is sent into photomultiplier 5 through optical fiber 2 transmission, so that carry out next step processing.
For fibre system, light emitting diode is a kind of important light source, and the radiation that it takes place has the spectral range that is rather narrow, and has enough output powers and useful power conversion efficiency.The used optical fiber 2 of this device is Y type quartz glass optical fibers, and what it adopted ultraviolet signal transmission branch road is silica fibre, and what fluorescence signal transmission branch road was adopted is glass optical fiber.This optical fiber structure has just met the designing requirement of native system.Because for the less ultraviolet light of wavelength, frequency is higher, its to the requirement of transmission medium also than higher, so adopt silica fibre to transmit, and for the long fluorescence of wavelength, its frequency is lower, so to the requirement of transmission medium also than higher, so adopt glass optical fiber to transmit.
Signal processing circuit of the present utility model is seen Fig. 3, and the utility model adds a signal screening amplification treatment circuit between the input end of photomultiplier and A/D converter, makes signal meet the area requirement of its analog input when entering A/D converter.
Can signal be divided three classes according to the size of signal, classification reference point voltage is respectively 0.9V and 9V for this reason.Like this, the output signal of photomultiplier just be divided into less than 0.9 volt, greater than 0.9 volt less than 9 volts and greater than 9 volt of three class.For the signal that belongs to different range, especially the signal outside the analog input scope of A/D converter wishes to make within its analog input scope that is in A/D converter by it being done suitable processing and amplifying.Therefore, for less than 0.9 volt signal, can make that its gain is 10; For greater than 0.9 volt less than 9 volts signal, can make that its gain is 1; For greater than 9 volts signal, can make that its gain is 1/10.Like this, the scope of total output Vo of circuit just within 0～10V, satisfies the analog input requirement of AD.
The signal screening amplification treatment circuit can be divided into two parts by function, and the previous section circuit is a threshold circuit that comprises U1A and two voltage comparators of U1B.By reconciling input resistance, making the negative input end of U1A is 9V, and the positive input terminal of U1B is 0.9V.So, the scope under the input signal just can be determined by output signal A, the B of threshold circuit.Signal A, B send into microprocessor on the one hand, are used for identifying selected gain; Signal A, B be also as two four switching signals of selecting a switch chip 4052 on the other hand, selects which gain in order to the output Vo of decision-making circuit.The aft section circuit is the amplifier of three groups of different gains, and its size is respectively 10,1 and 1/10.For the input signal of different sizes,, just can select to select that road of a switch chip 4052 to export by two four through after the judgement of threshold circuit.
The output voltage of threshold circuit enters 74LS08 through behind the electric resistance partial pressure.The output voltage of LM324 fluctuates in a scope, for the I/O mouth of protecting microprocessor not because of voltage fluctuation damages, so need meet a 74LS08 voltage of fluctuation is become stable level.What also should be noted that a bit is, the 74LS08 operating voltage is 5V, so the size of the input signal of 74LS08 can not surpass 5V, adds a bleeder circuit in its front with protection 74LS08 for this reason.
Two four select the operational amplifier LM324 of a switch chip 4052 back to bear the effect of amplifier and follower.Since the voltage amplification factor of follower less than 1 but be bordering on 1, input voltage is identical with output voltage, and input resistance height and output resistance is low, so be 1 and 1/10 circuit for gain, adopts follower to export.It for gain 1/10 circuit; the realization of its gain realizes by electric resistance partial pressure; rather than realize by amplifier; 4052 operating voltage was 12V because the back connect; so the size of its input signal can not surpass this value; therefore, utilize electric resistance partial pressure can also protect 4052, its input value can not gone beyond the scope.
Theoretically, if input signal smaller or equal to 0.9V, Ci Shi signal gain is calculated according to 10 so, if signal between 0.9V to 9V, Ci Shi signal gain is calculated according to 1 exactly so.When signal the time, then gain and calculate according to 1/10 more than or equal to 9V.Should yield value calculate according to which actually in the time of near signal is in critical point? this is to be determined by the value of scope distinguishing mark signal A, B.For example, when signal was 0.9V, if A=0, B=0, yield value got 1 so; If A=0, B=1, yield value gets 10 so.Because signal value finally can be reverted to initial value by microprocessor, so handle so can not influence final result at all.
In actual treatment, the handled yield value of microprocessor is come definite by experiment, and is not the ideal value that obtains according to Theoretical Calculation.In order to improve system accuracy, microprocessor can utilize software that this value is revised when this yield value is handled, in the hope of reaching higher precision.
Because the built-in negative high voltage of photomultiplier, its output voltage are negative value, and the input signal that the amplifying circuit of back requires be on the occasion of, so before signal carries out processing and amplifying, need add an anti-phase treatment circuit.
Because in signal processing circuit, the operating voltage of operational amplifier be on the occasion of, and the output voltage of photomultiplier is a negative value, for this reason before signal enters the examination amplification treatment circuit, need be through an anti-phase treatment circuit, make the output signal of photomultiplier meet the input requirement of next stage circuit, yield value A
K=-1.
Claims (3)
1. vitamin fluorescence detecting system comprises light source, is loaded with microwell plate, signal pickup assembly, signal processing circuit and the microprocessor of reagent, it is characterized in that described microwell plate is placed on the scanister; Described scanister is subjected to the control of microprocessor, can be along level and movement in vertical direction; Described light source is a Halogen lamp LED, and the light of its generation generates uv excitation light by wavelength for the 345nm narrow band pass filter; Described signal pickup assembly comprises narrow band pass filter, optical fiber, collector lens, fluorescence filter sheet and photomultiplier, described optical fiber is Y type conduction optical fiber, its public port is near described microwell plate, a bifurcated port links to each other with described light source, uv excitation light is radiated on the reagent that places microwell plate after conducting Optical Fiber Transmission and collector lens through the Y type, the fluorescence of the 485nm wavelength that is excited by reagent place goes out from another divergent ends oral instructions of Y type conduction optical fiber, and after the fluorescence filter sheet filters, be admitted to photomultiplier; Described signal processing circuit is used for converting the fluorescence signal that described photomultiplier detects to electric signal, and to sending into microprocessor after this electric signal processing.
2. vitamin fluorescence detecting system according to claim 1 is characterized in that, described Y type conduction optical fiber is quartz glass optical fiber, and the branch road of the ultraviolet light of its transmission light source emission is a silica fibre, is glass optical fiber and fluorescence signal is transmitted branch road.
3. vitamin fluorescence detecting system according to claim 1, it is characterized in that, described signal processing apparatus comprises anti-phase treatment circuit, screens amplification treatment circuit and microprocessor and A/D change-over circuit, is admitted to the examination amplification treatment circuit behind the anti-phase treatment circuit of electric signal process by described photomultiplier output; Described examination amplification treatment circuit comprises threshold circuit, on-off circuit, 10 times of amplifying circuits, 1 times of amplifying circuit and 1/10 times of amplifying circuit, described threshold circuit is by two voltage comparators, produce 9V and two threshold voltage signals of 0.9V, these two signals are admitted to microprocessor and on-off circuit simultaneously respectively, by on-off circuit according to a circuit in 10 times of amplifying circuits of two threshold voltage signal gatings, 1 times of amplifying circuit and the 1/10 times of amplifying circuit; Be admitted to microprocessor behind the output process A/D change-over circuit of described examination amplification treatment circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010200432568U CN201724904U (en) | 2010-01-14 | 2010-01-14 | Vitamin fluorescence detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010200432568U CN201724904U (en) | 2010-01-14 | 2010-01-14 | Vitamin fluorescence detection system |
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| Publication Number | Publication Date |
|---|---|
| CN201724904U true CN201724904U (en) | 2011-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010200432568U Expired - Fee Related CN201724904U (en) | 2010-01-14 | 2010-01-14 | Vitamin fluorescence detection system |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2509716A (en) * | 2013-01-09 | 2014-07-16 | Internat Moisture Analysers Ltd | Optical chemical analyser and liquid depth sensor |
-
2010
- 2010-01-14 CN CN2010200432568U patent/CN201724904U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2509716A (en) * | 2013-01-09 | 2014-07-16 | Internat Moisture Analysers Ltd | Optical chemical analyser and liquid depth sensor |
| GB2509716B (en) * | 2013-01-09 | 2018-07-04 | International Moisture Analysers Ltd | Spatial Interference Fourier Transform Raman chemical analyser |
| US10041880B2 (en) | 2013-01-09 | 2018-08-07 | International Moisture Analysers Limited | Optical chemical analyser and liquid depth sensor |
| US10508989B2 (en) | 2013-01-09 | 2019-12-17 | International Moisture Analysers Limited | Optical chemical analyser and liquid depth sensor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110126 Termination date: 20140114 |