CN201464473U - Chromatography detecting device with main reaction channel and auxiliary reaction channel - Google Patents
Chromatography detecting device with main reaction channel and auxiliary reaction channel Download PDFInfo
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- CN201464473U CN201464473U CN2009200927661U CN200920092766U CN201464473U CN 201464473 U CN201464473 U CN 201464473U CN 2009200927661 U CN2009200927661 U CN 2009200927661U CN 200920092766 U CN200920092766 U CN 200920092766U CN 201464473 U CN201464473 U CN 201464473U
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Abstract
The utility model discloses a chromatography detecting device with a main reaction channel and an auxiliary reaction channel, which comprises an outer box. An upper cover is arranged at the upper part of the outer box. A chromatography detecting device carrier which is arranged at the bottom part of the outer box comprises the main reaction channel and at least one auxiliary reaction channel. The chromatography reaction detecting device at least comprises two reaction channels, and samples and marked objects can be diffused to a solid-phase reaction film by different channels so as to carry out reaction and display results on the solid-phase reaction film. Being provided with a plurality of channels, the chromatography detecting device can improve the reaction sensitivity and specificity, reduce mutual interference, and avoid low efficiency as well as low compatibility of reaction by single channel.
Description
Technical field
The utility model relates to a kind of chromatography pick-up unit of establishing reaction main channel and reaction secondary channels that is applicable to immune detection, gene diagnosis and biochip.
Background technology
At present, accurate, the method for quick based on diseases and conditions mainly contains immunochromatography, gene diagnosis and biochip.Immunochromatography is a kind of easy, detection method fast, can detect in real time at any time and any place; Gene diagnosis mainly is to carry out gene or detection of nucleic acids, mainly is based on the genetic test or the gene diagnosis of PCR or isothermal duplication at present; Biochip mainly comprises genetic chip and protein-chip.Gene diagnosis and biochip fast, accurately, the methodology of real-time online and the development of product, be the molecular diagnosis field analysis of Development, press for this class technology and product at present and enter clinically, enter family.
But, immunochromatography, gene diagnosis and biochip chromatography fast and accurately detect and also to be faced with many challenges, press in the following aspects to innovate and improve: (1) will tackle the detection needs of various samples: these samples comprise the complex sample of whole blood, serum, blood plasma, saliva, urine and food safety detection; (2) diversity of label and reagent: the detection of immunochromatography, gene diagnosis and biochip may be the detection of single component, but majority may be multicomponent detection, and this just needs multiple label and plurality of reagents composition to participate in reaction; (3) detection sensitivity and specific needs: multiple composition mixes and may influence each other, thereby influences the sensitivity and the specificity of its detection, separately shunts sensitivity and the specificity that reacts one by one and can improve reaction; (4) needs of detection by quantitative: detection by quantitative needs result high-visible, no background interference.
Flash chromatography detection based on the film holder is the direction that the present and the future is developed with diagnosis, but the technical matters that will solve mainly contains: low usefulness of (1) single pathway reaction and low compatibility; (2) be difficult to realize preferably that genetic chip and protein-chip are quick, flux detects; (3) be difficult to carry out detection by quantitative accurately; (4) biochip liquid phase reagent instability, shelf life weak point; (5) long, application of sample (referring to add sample and reagent) repeatedly of many times of gene diagnosis and biochip complicated operation, reactions steps, the multistep washing.
The utility model content
The purpose of this utility model provides a kind of chromatography pick-up unit of establishing reaction main channel and reaction secondary channels that is applicable to immune detection, gene diagnosis and biochip, and the chromatography of especially suitable quick, sensitive immunochromatography, nucleic acid or genetic test, genetic chip and protein-chip based on film detects.
For achieving the above object, the utility model is by the following technical solutions: the chromatography pick-up unit of establishing reaction main channel and reaction secondary channels, comprise outer box, outer box top is provided with loam cake, the bottom of described outer box is provided with chromatography reaction checking device carrier, described chromatography reaction checking device carrier comprises reaction main channel and at least one reaction secondary channels, described reaction main channel comprises a solid support, the upper surface of solid support is connected with the reactant pad from top to bottom successively, solid phase reaction film and absorbent material, described reaction secondary channels comprises a solid support, the upper surface of solid support is provided with the reactant pad, and the bottom of reactant pad connects with the solid phase reaction symphysis of reaction main channel; The reactant pad of the reactant pad of described reaction main channel and reaction secondary channels has at least one to be sample pad, and other reactant pad is labeling pad, sample pad or reagent pad.
When described reaction secondary channels was one, the reaction main channel was respectively labeling pad and sample pad or is respectively sample pad and labeling pad with the reactant pad of reaction secondary channels.
Cover on described and be provided with application of sample window and view window, the position of view window is corresponding with the position of solid phase reaction film on the reaction main channel, and the position of the application of sample window reactant pad position with reaction main channel and reaction secondary channels respectively is corresponding.
When described reaction secondary channels was two, the reaction secondary channels laid respectively at the both sides of reaction main channel, and the reactant pad of reaction main channel is a labeling pad, and the reactant pad of two reaction secondary channels is respectively sample pad and reagent pad.
Cover on described and be provided with application of sample window and view window, the position of view window is corresponding with the position of solid phase reaction film on the reaction main channel, and the position of application of sample window is corresponding with the position of the sample pad of the labeling pad of reaction main channel and reaction secondary channels, reagent pad respectively.
Chromatography reaction checking device of the present utility model is provided with two reaction channels at least, and sample can diffuse to the solid phase reaction film by different passages with label, reaction and display result on the solid phase reaction film; A plurality of reaction secondary channels are set when needing to use other reagent, and sample, label and reagent diffuse to the solid phase reaction film through different passages respectively, react then and display result.The utility model is provided with a plurality of passages, can improve the sensitivity and the specificity of reaction, reduces the phase mutual interference, avoids the low usefulness and the low compatibility of single pathway reaction.
The utility model both can be used for immunochromatography, can be used for gene diagnosis again, can also be used for biochip reaction, achieved many things at one stroke, and had accurate, quick, easy characteristics; Can realize easily that gene diagnosis, the many indexs of biochip, multicomponent flux detect; Can carry out the particularly detection by quantitative of many indexs of single index exactly, this has important practical value to serious infectious diseases (hepatitis, acquired immune deficiency syndrome (AIDS) etc.), major disease (cardiovascular and cerebrovascular disease, malignant tumour) and food safety detection (residues of pesticides, residue of veterinary drug etc.) etc.; The labeling pad shelf life can be 1～2 year, mainly preserves in the time of 4 ℃, but can preserve and the normal temperature transportation at normal temperature; Reactions steps is few, the reaction time is short, can obtain a result in 5～20 minutes, is suitable for the real-time online immediate reaction especially.
Description of drawings
Fig. 1 is the structural representation of embodiment 1;
Fig. 2 is the structural representation of the chromatography reaction checking device carrier of embodiment 1;
Fig. 3 is the structural representation of embodiment 1 reaction main channel;
Fig. 4 is the structural representation of embodiment 1 reaction secondary channels;
Fig. 5 is the structural representation of embodiment 2;
Fig. 6 is the structural representation of the chromatography reaction checking device carrier of embodiment 2;
Fig. 7 is the structural representation of embodiment 2 reaction main channels;
Fig. 8 is the structural representation of embodiment 2 reaction secondary channels 18.
Embodiment
Embodiment 1: twin-channel chromatography pick-up unit, as Fig. 1,2,3, shown in 4, comprise outer box 2, outer box 2 tops are provided with loam cake 1, the bottom of outer box 2 is provided with chromatography reaction checking device carrier 11, chromatography reaction checking device carrier 11 comprises reaction main channel 14 and a reaction secondary channels 15, reaction main channel 14 comprises a solid support 12, the upper surface of solid support 12 is connected with labeling pad 5 from top to bottom successively, solid phase reaction film 4 and absorbent material 22, reaction secondary channels 15 comprises a solid support 13, the upper surface of solid support 13 is provided with sample pad 8, the bottom of sample pad 8 is connected with the solid phase reaction film 4 of reaction main channel 14. and loam cake 1 is provided with application of sample window 7-1, application of sample window 7-2 and view window 3, the position of view window 3 is corresponding with the position of solid phase reaction film 4, and the position of application of sample window 7-1 and application of sample window 7-2 is corresponding with sample pad 8 positions of reaction secondary channels 15 with the labeling pad 5 of reaction main channel 14 respectively.
During use testing sample is added on the sample pad 8 through application of sample window 7-2, distilled water added on the labeling pad 5 through application of sample window 7-1 label is dissolved, label after testing sample and the dissolving diffuses on the solid phase reaction film 4 through different passages respectively, finally reaction and show testing result on solid phase reaction film 4.
Embodiment 2: three-channel chromatography pick-up unit, as Fig. 5,6,7, shown in 8, comprise outer box 2, outer box 2 tops are provided with loam cake 1, the bottom of outer box 2 is provided with chromatography reaction checking device carrier 11, chromatography reaction checking device carrier 11 comprises reaction main channel 16, reaction secondary channels 18 and reaction secondary channels 19, reaction secondary channels 18 and reaction secondary channels 19 are located at the both sides of reaction main channel 16 respectively, reaction main channel 16 comprises a solid support 12, the upper surface of solid support 12 is connected with labeling pad 5 from top to bottom successively, solid phase reaction film 4 and absorbent material 22, reaction secondary channels 18 and reaction secondary channels 19 include a solid support 13, the upper surface of the solid support 13 of reaction secondary channels 18 is provided with sample pad 10, the bottom of sample pad 10 is connected with the solid phase reaction film 4 of reaction main channel 16, the upper surface of the solid support 13 of reaction secondary channels 19 is provided with reagent pad 6, and the bottom of reagent pad 6 is connected with the solid phase reaction film 4 of reaction main channel 16.Loam cake 1 is provided with application of sample window 7-1, application of sample window 7-2, application of sample window 7-3 and view window 3, the position of view window 3 is corresponding with the position of solid phase reaction film 4, and the position of application of sample window 7-1, application of sample window 7-2 and application of sample window 7-3 is corresponding with the position of labeling pad 5, sample pad 10, reagent pad 6 respectively.
During use, testing sample is added on the sample pad 10 by application of sample window 7-2, required reaction reagent is added on the reagent pad 6 by application of sample window 7-3, distilled water is added on the labeling pad 5 through application of sample window 7-1 dissolves label, label after testing sample, required reaction reagent and the dissolving diffuses on the solid phase reaction film 4 through different passages respectively, finally reaction and demonstration testing result on solid phase reaction film 4.
Claims (5)
1. establish the chromatography pick-up unit of reaction main channel and reaction secondary channels, comprise outer box, outer box top is provided with loam cake, it is characterized in that: the bottom of described outer box is provided with chromatography reaction checking device carrier, described chromatography reaction checking device carrier comprises reaction main channel and at least one reaction secondary channels, described reaction main channel comprises a solid support, the upper surface of solid support is connected with the reactant pad from top to bottom successively, solid phase reaction film and absorbent material, described reaction secondary channels comprises a solid support, the upper surface of solid support is provided with the reactant pad, and the bottom of reactant pad connects with the solid phase reaction symphysis of reaction main channel; The reactant pad of the reactant pad of described reaction main channel and reaction secondary channels has at least one to be sample pad, and other reactant pad is labeling pad, sample pad or reagent pad.
2. the chromatography pick-up unit of establishing reaction main channel and reaction secondary channels as claimed in claim 1, it is characterized in that: when described reaction secondary channels was one, the reaction main channel was respectively labeling pad and sample pad or is respectively sample pad and labeling pad with the reactant pad of reaction secondary channels.
3. the chromatography pick-up unit of establishing reaction main channel and reaction secondary channels as claimed in claim 2, it is characterized in that: cover on described and be provided with application of sample window and view window, the position of view window is corresponding with the position of solid phase reaction film on the reaction main channel, and the position of the application of sample window reactant pad position with reaction main channel and reaction secondary channels respectively is corresponding.
4. the chromatography pick-up unit of establishing reaction main channel and reaction secondary channels as claimed in claim 1, it is characterized in that: when described reaction secondary channels is two, the reaction secondary channels lays respectively at the both sides of reaction main channel, the reactant pad of reaction main channel is a labeling pad, and the reactant pad of two reaction secondary channels is respectively sample pad and reagent pad.
5. the chromatography pick-up unit of establishing reaction main channel and reaction secondary channels as claimed in claim 4, it is characterized in that: cover on described and be provided with application of sample window and view window, the position of view window is corresponding with the position of solid phase reaction film on the reaction main channel, and the position of application of sample window is corresponding with the position of the sample pad of the labeling pad of reaction main channel and reaction secondary channels, reagent pad respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200927661U CN201464473U (en) | 2009-09-02 | 2009-09-02 | Chromatography detecting device with main reaction channel and auxiliary reaction channel |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200927661U CN201464473U (en) | 2009-09-02 | 2009-09-02 | Chromatography detecting device with main reaction channel and auxiliary reaction channel |
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| CN201464473U true CN201464473U (en) | 2010-05-12 |
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| CN2009200927661U Expired - Fee Related CN201464473U (en) | 2009-09-02 | 2009-09-02 | Chromatography detecting device with main reaction channel and auxiliary reaction channel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370871A (en) * | 2016-09-23 | 2017-02-01 | 武汉百美生物科技有限公司 | Test paper for testing chorionic gonadotropin in human saliva and preparation method thereof |
-
2009
- 2009-09-02 CN CN2009200927661U patent/CN201464473U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370871A (en) * | 2016-09-23 | 2017-02-01 | 武汉百美生物科技有限公司 | Test paper for testing chorionic gonadotropin in human saliva and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HEBEI YANDA MEDICAL RESEARCH INSTITUTE Free format text: FORMER OWNER: XIAO YUEYI Effective date: 20111013 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 450002 ZHENGZHOU, HENAN PROVINCE TO: 065201 LANGFANG, HEBEI PROVINCE |
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| TR01 | Transfer of patent right |
Effective date of registration: 20111013 Address after: 065201 Langfang city of Hebei province Sanhe Yanjiao Economic Development Zone on the west side of West Si Pu Lan Patentee after: HEBEI YANDA MEDICAL INSTITUTE Address before: 450002 Henan Province, Zhengzhou City Shakou road Zhongheng District No. 5 Building No. 8 hospital Patentee before: Xiao Leyi |
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| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100512 Termination date: 20130902 |