CN201302549Y - Non-destructive detecting device for cell components and intercellular components - Google Patents
Non-destructive detecting device for cell components and intercellular components Download PDFInfo
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- CN201302549Y CN201302549Y CNU200920136665XU CN200920136665U CN201302549Y CN 201302549 Y CN201302549 Y CN 201302549Y CN U200920136665X U CNU200920136665X U CN U200920136665XU CN 200920136665 U CN200920136665 U CN 200920136665U CN 201302549 Y CN201302549 Y CN 201302549Y
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- fluorescence excitation
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- extracellular matrix
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Abstract
The utility model relates to a non-destructive detecting device for cell components and intercellular components, which is characterized in that laser from a laser device enters into a scanning lens set of a scanner through a dichroic beam splitter, light beams from the scanning lens set are focused on a detected sample by an object lens; two-photon fluorescence excitation and secondary harmonic signals all generated by laser and the detected sample reversely penetrate through the same object lens to be collected, and then enter into a semi-transparent semi-reflective lens through the scanning lens set and the dichroic beam splitter to divide the signal transmission light formed by the two-photon fluorescence excitation and the secondary harmonic signals into two ways. The two ways of signal transmission light are converted into electric signals by a filter and a photoelectric multiplier, and are transmitted to an input terminal of a computer which is used for displaying microstructures of cells, elastic fiber and collagen fiber. The non-destructive detecting device for cell components and intercellular components can implement high-resolution, high-contrast, non-destructive, quick and real-time detection to microstructures of cells, elastic fiber and collagen fiber at molecular level.
Description
Technical field
The utility model relates to a kind of pair cell and the extracellular matrix composition carries out the device of lossless detection simultaneously.
Technical background
Present clinical medical pathology department every day all can pair cell, the microstructure of extracellular matrix principal ingredient collagenous fibres and elastic fibers is observed and detected.The Histopathology method that clinical medical detection method is normally traditional.The histopathologic slide that is used for liver histopathological analysis need obtain through a series of processes such as fixing, paraffin embedding, dewaxing, dyeing, these processes have not only been destroyed some relevant useful material true forms of tissue, and the time that needs is often long, each step institute's time spent all be with hour, or even calculate with the sky.In addition, sample disposal is incorrect and dyeing time is not sure all might cause check result to lose accuracy.If think to survey simultaneously the microstructure of cell, collagenous fibres and elastic fibers, needs are observed after using diverse dyestuff that histotomy is dyeed respectively again.Overcome the Histopathology method defective, seek a kind of high resolving power, microstructural new device harmless and that detect cell, collagenous fibres and elastic fibers in real time is target and the dream that a large amount of researchers and clinician make joint efforts always.
Summary of the invention
The purpose of this utility model provides a kind of pair cell and the extracellular matrix composition carries out the device of lossless detection simultaneously, this device adopts two-photon fluorescence excitation that light and matter interaction launch and second harmonic signal and pair cell and extracellular matrix composition microstructure are carried out the while lossless detection, can be on molecular level the microstructure of pair cell, collagenous fibres and elastic fibers carry out high resolving power, high-contrast, harmless, quick and real-time detection.
The technical solution of the utility model is achieved in that the light that laser instrument sends incides by a double-colored spectroscope on the scanning mirror group of scanner, the light beam by the scanning mirror group by object lens focusing on test sample; Laser is oppositely collected by identical object lens with two-photon fluorescence excitation and second harmonic signal that test sample produces, incide on the semi-transparent semi-reflecting lens by aforementioned scanning mirror group and double-colored spectroscope again, the light that transmits of two-photon fluorescence excitation and second harmonic signal composition is divided into two-way, leading up to filter plate 1 and photomultiplier 1 converts the second harmonic signal of collagenous fibres to electric signal A, leading up to filter plate 2 and photomultiplier in addition becomes electric signal B with the two-photon fluorescence excitation conversion of signals of cell and elastic fibers, described electric signal A and electric signal B are connected to input end and computer respectively, at last by computing machine while showed cell, the microstructure of elastic fibers and collagenous fibres.
Remarkable advantage of the present utility model is: its microstructure of input of utilizing the endogenous molecule interaction generation of laser and cell and extracellular matrix composition, therefore, do not need processing such as dyeing, directly can realize the high resolving power to sample, harmless and detection in real time.Though the microstructural detection of cell and elastic fibers all is by the two-photon fluorescence excitation signal, but the structure of the two has tangible difference, with the human body is example, the minimum diameter of human body cell is approximately 4 μ m, the similar sphere of shape, and under the Electronic Speculum, the diameter of elastic fibers has only 1-3nm, become wavy, therefore, the microstructure of the two can obviously distinguish, if select suitable optical maser wavelength, make collagenous fibres based on the emission second harmonic signal, can obtain the high-contrast microstructure of cell, collagenous fibres and elastic fibers.In addition, the use of scanner makes device have the function of fast detecting.
Description of drawings
Fig. 1 is the organigram of the utility model embodiment.
Embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is further elaborated, so that the utility model becomes apparent.
As shown in Figure 1, the light that laser instrument sends incides by a double-colored spectroscope on the scanning mirror group of scanner, the light beam by the scanning mirror group by object lens focusing on test sample; Laser is oppositely collected by identical object lens with two-photon fluorescence excitation and second harmonic signal that test sample produces, incide on the semi-transparent semi-reflecting lens by aforementioned scanning mirror group and double-colored spectroscope again, the light that transmits of two-photon fluorescence excitation and second harmonic signal composition is divided into two-way, leading up to filter plate 1 and photomultiplier 1 converts the second harmonic signal of collagenous fibres to electric signal A, leading up to filter plate 2 and photomultiplier in addition becomes electric signal B with the two-photon fluorescence excitation conversion of signals of cell and elastic fibers, described electric signal A and electric signal B are connected to input end and computer respectively, at last by computing machine while showed cell, the microstructure of elastic fibers and collagenous fibres.
In most preferred embodiment of the present utility model, the wavelength of laser instrument should be selected 810nm for use, this moment collagenous fibres based on the emission second harmonic signal, avoid pair cell particularly to the detection of elastic fibers microstructure high-contrast.If the wavelength of laser instrument is selected 810nm for use, double-colored spectroscope need see through the incident light greater than 700nm, reflects the emission light less than 700nm simultaneously.The filter range of filter plate 1 is realized the collection to the collagenous fibres second harmonic signal between 400-410nm, and the filter range of filter plate 2 is realized the collection of pair cell and elastic fibers two-photon fluorescence excitation signal between 440-650nm.
As long as above-mentioned laser instrument satisfies the condition of light and cell, collagenous fibres and elastic fibers interaction generation two-photon fluorescence excitation and second harmonic signal, can be dissimilar laser instruments, as different types of laser instruments such as femtosecond laser instrument, picosecond lasers.
Be provided with light intensity control and total reflective mirror between above-mentioned laser instrument and double-colored spectroscope, above-mentioned light intensity control is made up of 1/2nd wave plates and Glan prism.
The utility model is reasonable in design, is skillfully constructed, and has vast potential for future development and bigger dissemination.
Claims (5)
1. pair cell and extracellular matrix composition carry out the device of lossless detection simultaneously, it is characterized in that: the light that laser instrument sends incides by a double-colored spectroscope on the scanning mirror group of scanner, the light beam by the scanning mirror group by object lens focusing on test sample; Laser is oppositely collected by identical object lens with two-photon fluorescence excitation and second harmonic signal that test sample produces, incide on the semi-transparent semi-reflecting lens by aforementioned scanning mirror group and double-colored spectroscope again, the light that transmits of two-photon fluorescence excitation and second harmonic signal composition is divided into two-way, leading up to filter plate 1 and photomultiplier 1 converts the second harmonic signal of collagenous fibres to electric signal A, leading up to filter plate 2 and photomultiplier in addition becomes electric signal B with the two-photon fluorescence excitation conversion of signals of cell and elastic fibers, described electric signal A and electric signal B are connected to input end and computer respectively, at last by computing machine while showed cell, the microstructure of elastic fibers and collagenous fibres.
2. pair cell according to claim 1 and extracellular matrix composition carry out the device of lossless detection simultaneously, it is characterized in that: the optical maser wavelength that described laser instrument sends equals 810nm, described double-colored spectroscope can see through the incident light greater than 700nm, reflect emission light simultaneously less than 700nm, the filter range of filter plate 1 is between 400-410nm, realization is to the collection of collagenous fibres second harmonic signal, and the filter range of filter plate 2 is realized the collection of pair cell and elastic fibers two-photon fluorescence excitation signal between 440-650nm.
3. pair cell according to claim 1 and extracellular matrix composition carry out the device of lossless detection simultaneously, and it is characterized in that: described laser instrument is femto-second laser or picosecond laser.
4. pair cell according to claim 1 and extracellular matrix composition carry out the device of lossless detection simultaneously, it is characterized in that: be provided with light intensity control and total reflective mirror between described laser instrument and double-colored spectroscope.
5. pair cell according to claim 4 and extracellular matrix composition carry out the device of lossless detection simultaneously, it is characterized in that described light intensity control is made up of 1/2nd wave plates and Glan prism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200920136665XU CN201302549Y (en) | 2009-02-12 | 2009-02-12 | Non-destructive detecting device for cell components and intercellular components |
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| CNU200920136665XU CN201302549Y (en) | 2009-02-12 | 2009-02-12 | Non-destructive detecting device for cell components and intercellular components |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102297854A (en) * | 2011-05-23 | 2011-12-28 | 公安部第一研究所 | High-efficiency multi-mode laser-induced fluorescence optical path exciting system |
| CN103616359A (en) * | 2013-12-04 | 2014-03-05 | 中国检验检疫科学研究院 | Fluorescent quantitative detection method for green fluorescent protein (GFP) |
| CN104596964A (en) * | 2015-02-03 | 2015-05-06 | 黑龙江大学 | Device and method for measuring transient absorbency of semitransparent thin film material |
| CN104758058A (en) * | 2015-03-11 | 2015-07-08 | 苏州大学 | Synchronous pulse laser microimaging observation device for mechanical stress deformation of blood cells |
| CN105738254A (en) * | 2016-02-03 | 2016-07-06 | 苏州大学 | Mechanobiological coupling testing system and method |
| CN106841145A (en) * | 2017-02-28 | 2017-06-13 | 中国中医科学院医学实验中心 | Second Harmonic Imaging combined with fluorescent is imaged on the application in positioning material Transdermal absorption |
| CN106990077A (en) * | 2017-06-15 | 2017-07-28 | 福建师范大学 | It is a kind of based on the micro- high throughput screening system with microfluidic chip technology of multi-photon |
| CN109073539A (en) * | 2016-05-03 | 2018-12-21 | 文塔纳医疗系统公司 | System and method for monitoring reagent concentration |
| CN109813654A (en) * | 2019-02-20 | 2019-05-28 | 北京工业大学 | The time discrimination measurement device of material surface state second_harmonic generation |
| CN110082330A (en) * | 2019-05-16 | 2019-08-02 | 福建师范大学 | Combined system is imaged in a kind of laser optical tweezer Raman spectrum and multi-photon |
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2009
- 2009-02-12 CN CNU200920136665XU patent/CN201302549Y/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102297854A (en) * | 2011-05-23 | 2011-12-28 | 公安部第一研究所 | High-efficiency multi-mode laser-induced fluorescence optical path exciting system |
| CN103616359A (en) * | 2013-12-04 | 2014-03-05 | 中国检验检疫科学研究院 | Fluorescent quantitative detection method for green fluorescent protein (GFP) |
| CN104596964A (en) * | 2015-02-03 | 2015-05-06 | 黑龙江大学 | Device and method for measuring transient absorbency of semitransparent thin film material |
| CN104596964B (en) * | 2015-02-03 | 2017-05-31 | 黑龙江大学 | A kind of translucent thin membrane material transient absorbance degree measurement apparatus and method |
| CN104758058A (en) * | 2015-03-11 | 2015-07-08 | 苏州大学 | Synchronous pulse laser microimaging observation device for mechanical stress deformation of blood cells |
| CN105738254A (en) * | 2016-02-03 | 2016-07-06 | 苏州大学 | Mechanobiological coupling testing system and method |
| CN105738254B (en) * | 2016-02-03 | 2019-07-12 | 苏州大学 | A kind of Mechanobiology coupling testing system and method |
| CN109073539A (en) * | 2016-05-03 | 2018-12-21 | 文塔纳医疗系统公司 | System and method for monitoring reagent concentration |
| CN106841145A (en) * | 2017-02-28 | 2017-06-13 | 中国中医科学院医学实验中心 | Second Harmonic Imaging combined with fluorescent is imaged on the application in positioning material Transdermal absorption |
| CN106841145B (en) * | 2017-02-28 | 2020-06-12 | 中国中医科学院医学实验中心 | Application of second harmonic imaging and fluorescence imaging in positioning substance transdermal absorption |
| CN106990077A (en) * | 2017-06-15 | 2017-07-28 | 福建师范大学 | It is a kind of based on the micro- high throughput screening system with microfluidic chip technology of multi-photon |
| CN109813654A (en) * | 2019-02-20 | 2019-05-28 | 北京工业大学 | The time discrimination measurement device of material surface state second_harmonic generation |
| CN109813654B (en) * | 2019-02-20 | 2021-10-01 | 北京工业大学 | Time-resolved measuring device for material surface state second harmonic generation |
| CN110082330A (en) * | 2019-05-16 | 2019-08-02 | 福建师范大学 | Combined system is imaged in a kind of laser optical tweezer Raman spectrum and multi-photon |
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Granted publication date: 20090902 Termination date: 20120212 |