CN201272805Y - Novel fermentation tank - Google Patents
Novel fermentation tank Download PDFInfo
- Publication number
- CN201272805Y CN201272805Y CNU2008201419099U CN200820141909U CN201272805Y CN 201272805 Y CN201272805 Y CN 201272805Y CN U2008201419099 U CNU2008201419099 U CN U2008201419099U CN 200820141909 U CN200820141909 U CN 200820141909U CN 201272805 Y CN201272805 Y CN 201272805Y
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- Prior art keywords
- tank
- film
- fermentor tank
- membrane
- fermentation
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- Expired - Fee Related
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- 238000000855 fermentation Methods 0.000 title claims abstract description 32
- 230000004151 fermentation Effects 0.000 title claims abstract description 29
- 239000012528 membrane Substances 0.000 claims abstract description 47
- 239000000463 material Substances 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims description 33
- 238000005374 membrane filtration Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 238000001471 micro-filtration Methods 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 238000000108 ultra-filtration Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 3
- 239000007791 liquid phase Substances 0.000 abstract description 3
- 239000002245 particle Substances 0.000 abstract description 3
- 238000005842 biochemical reaction Methods 0.000 abstract description 2
- 238000004113 cell culture Methods 0.000 abstract description 2
- 230000010261 cell growth Effects 0.000 abstract description 2
- 230000019522 cellular metabolic process Effects 0.000 abstract description 2
- 230000008878 coupling Effects 0.000 abstract description 2
- 238000010168 coupling process Methods 0.000 abstract description 2
- 238000005859 coupling reaction Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 230000004907 flux Effects 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 238000010907 mechanical stirring Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
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- 244000005700 microbiome Species 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a novel fermentation tank, which comprises a tank body, a membrane filter and a pipe, wherein the membrane filter is arranged inside the tank body, one end of the pipe in the tank body is connected with the membrane filter and the other end extends to the outside of the tank body or extends to the outside of the tank body through a hollow agitating shaft. The membrane filter can be arranged on the tank wall through the pipe or can be arranged on the hollow agitating shaft, and an angle ranging from 0 degrees to 90 degrees can be formed by longitudinal axe of the membrane filter and the tank. Performing membrane separation along with bio-fermentation, positive pressure in the fermentation tank or/ and negative pressure of the outside of the tank enables liquid-phase material to penetrate through the membrane to reach other devices outside the fermenting tank via a pipe, and then suspended cells and intercepted particles are stopped to stay in the fermentation tank for continuous fermentation. By utilizing characteristics of combination and separation between the fermentation tank and the membrane, the fermentation tank realizes coupling between cell culture and product extraction in the tank without affecting growth and metabolism of cells, has good reaction effect, stable biochemical reaction system, low cost and small investment, and is adaptable to application of biological reaction.
Description
Technical field
The utility model relates to a kind of zymetology or microbiology device, has especially adopted the biological fermentation tank of membrane separation technique.
Background technology
Membrane separation technique has following advantage: carry out mild condition at normal temperatures; Material does not have phase transformation; Energy consumption is low; Equipment is simple, convenient operation and control.Film and membrane technique have obtained in fields such as chemical industry, medicine, food-drink, electronics and biochemical industries using more widely.Film and bio-reactor organically combine form a kind of integrated system---(MembraneReactor MBR), has become hot research in recent years to membrane bioreactor.
So far existing multiple membrane bioreactor is studied.Membrane bioreactor the earliest is the application at water treatment, also be the most sophisticated membrane bioreactor on using up to now, but this class reactor is not suitable for usually said biological fermentation, and is different with purpose of the present invention.Existing membrane bioreactor still can not satisfy the requirement of biological fermentation production application fully." mineral membrane biological reaction apparatus " (ZL 03221776.5) of Lin Xiao and Lu Fuping " adopting mineral membrane to realize the method for microorganism in situ separation and fermentation " (CN 1908176A) places film outside the fermentor tank, film is connected by pipeline with fermentor tank, belongs to film circulating fermentation jar; The shortcoming of this class membrane bioreactor is a cell in membrane module and pipeline the time, and conditions such as dissolved oxygen are uncontrollable, and the pump convection cell produces violent stirring, and easily cell growth and metabolism damage, and destroy the stability of biochemical reaction system easily.Also have the people that the organic hollow tunica fibrosa is placed fermentor tank, but the physical strength of this class film is low, flux is little, and treatment capacity is little, easy cleaning not, and poor chemical stability, work-ing life is short, does not more accommodate in the macro-organism reactor and uses.
The utility model content
The technical problems to be solved in the utility model provides a kind of novel fermentation jar, and it more meets the use operational requirement of bio-reactor from designing the shortcoming that has solved above-mentioned membrane bioreactor, and cost is low, the investment is little, simple in structure.
The utility model is achieved through the following technical solutions:
A kind of novel fermentation jar is the integral type film fermentor tank, comprises tank body and from the pipeline of fermentor tank pass-out, the end that described pipeline feeds tank body is connected with film filter, the other end of pipeline with jar be interlinked to the outside.
Described film filter is board-like film or tubular membrane, and wherein the material of film can be organic membrane or mineral membrane; Membrane pore size in the described film filter is molecular weight 1000 dalton～10 μ m; Described film filter is provided with direction and becomes 0-90 ° of angle with the longitudinal axis of jar in fermentor tank; The number of film filter is at least two; Film filter can be by pipe connection on tank wall; Described fermentor tank can be stirred-tank fermenter, comprises stir shaft and is installed in stirring arm on the stir shaft, the understirring type of described stir shaft for leading to the last stirring-type that gets off from tank deck or leading to up at the bottom of the jar; Described stir shaft can be hollow stir shaft, and film filter is connected with hollow stir shaft by pipeline and leads to outside the jar; Described fermentor tank also can be airlift fermentor or mechanical stirring self-suction fermentor, and fermentor tank can also be an anaerobic fermentation tank.Film is immersed in fermentor tank in the fermented liquid, sees through liquid in the film, passes to outside the jar by the pipeline that connects with symphysis, and pipeline can be to be connected with an end of film to feed a jar outer individual tubes, also can be to be connected two pipelines that pass to respectively outside the jar with the two ends of film.This pipeline has valve, can connect devices such as pump, storage tank, chromatographic column, membrane module, fermentor tank, reactor, concentrating unit, crystallization apparatus, extraction plant and distillation tower.
Airlift fermentor can be the interior circulation type or the external loop type of band circulating pipe, also can be the airlift fermentor of not being with circulating pipe, and the material of film is metal or pottery or resin, also can be matrix material; The shape of the cross section of tubular membrane can be circle, trilateral, water droplet shape, streamlining.Film can pass through pipeline or/and mounting block is fixed on the wall of fermentor tank.The pipeline of junctional membrane and fermentor tank passes the outer pipe connection of fermentor walls and fermentor tank.Film can be fixed on the stir shaft of fermentor tank by pipeline and/or mounting block, rotates with stir shaft, and mounting block rises and supports and fixed action, prevents that influence that film stirs liquid because of gravity, rotation and periphery from becoming flexible or come off from stir shaft.Film is by the pipeline of junctional membrane and stir shaft, and with the pipe connection in the hollow stir shaft, the pipeline in the hollow stir shaft is connected with jar pipeline outward again.Film filter and fermented liquid are realized relative movement in the membrane sepn process, constantly having avoided the film surface to form concentration polarization better in the motion, make film keep the higher flux that soaks into, thereby keep good filter effect on the one hand; Also can play the effect of stirring arm on the other hand, it is more abundant that fermented liquid is mixed.
Film can also be the combination of above-mentioned unlike material, different shapes, different fixing angle.The stationary arrangement mode of film can be single, individual layer, individual pen, also can be many rows, multilayer, multi-turn.Film with the mode of connection of pipeline and mounting block can be weld, be threaded, flange is connected, socket connection, fast-assembling clamp connection, also can be the combination of above-mentioned mode of connection.
The utility model places film in the fermentor tank, is immersed in the fermented liquid, in biological fermentation, carries out the membrane sepn operation.The requirement of biological fermentation is satisfied in the mounting means of film and the selection of film fully.Biomass cells does not enter film and reaches in the pipeline that connects with symphysis, and the growth metabolism condition of cell can be controlled well; The aseptic technique of fermentor tank can guarantee.Malleation in the fermentor tank arrives other outer device of film fermentation jar or/and a jar outer negative pressure makes liquid phase material see through film by pipeline, and the cell of suspension and trapped particle are trapped within the fermentor tank.Because be that external-compression type filters, the stirring of machinery or pressurized gas is arranged, make fermented liquid be in agitated conditions always, fermented liquid forms cross-flow around film, avoided forming concentration polarization on the film surface, makes film keep higher flux, prolongs the life cycle of film.In case of necessity, can oppositely in fermentor tank, feed liquid or gas, film is recoiled, make film recover flux by film.
The common practise that specifically is configured to those skilled in the art of the several types of fermentor tank also can be found in coherent reference data such as " biotechnology equipment ", does not influence concrete enforcement of the present invention.
Novel fermentation jar of the present utility model utilizes fermentor tank and membrane sepn bonded characteristics, can realize following technique effect: the 1. coupling of cell cultures and product extraction, and the original position of product is separated; 2. the recycling of cell and enzyme; 3. continuously ferment; 4. the high-density culture of cell; 5. reduce the matter and energy consumption of fermentation; 6. improve transformation efficiency, enhance productivity, improve plant factor; 7. reduce fermentation broth viscosity, solve the dissolved oxygen difficult problem of high viscosity fermentation; 8. simplify the technological process of production, improve the automatization level of production and the stability of production; 9. the reactive system to there being product to suppress is coupled by reaction-separations and can reduces production concentration in the reactant, alleviates or eliminates the product inhibition; 10. realize that fermentation and product are extracted in airtight carrying out in container and the pipeline, reduce external pollution and interference; The purpose that final realization reduces production costs.Present method is utilized fermentor tank and film integrated advantage, has simplified schedule of operation, and makes operational condition be easier to control, and Reaction Separation is gentle more, and the product purity that obtains is higher.
Description of drawings
Fig. 1 is the structural representation of the stirred-tank fermenter of the film that is arranged in parallel of built-in and the fermentor tank longitudinal axis.
Fig. 2 be Fig. 1 fermentor tank along A-A to sectional view, film is board-like film.
Fig. 3 be Fig. 1 fermentor tank along A-A to sectional view, film is a tubular membrane.
The structural representation of the stirred-tank fermenter of Fig. 4 the is built-in film vertical or angled with the fermentor tank longitudinal axis.
Fig. 5 be Fig. 4 fermentor tank along A-A to sectional view, film is board-like film.
Fig. 6 be Fig. 4 fermentor tank along A-A to sectional view, film is a tubular membrane.
Fig. 7 is the structural representation that the film and the fermentor tank longitudinal axis are connected the stirred-tank fermenter on the stir shaft abreast.
Fig. 8 be Fig. 7 fermentor tank along A-A to sectional view, film is board-like film.
Fig. 9 be Fig. 7 fermentor tank along A-A to sectional view, film is a tubular membrane.
Figure 10 is the structural representation that the film and the fermentor tank longitudinal axis vertically are connected the stirred-tank fermenter on the stir shaft.
Figure 11 be Figure 10 fermentor tank along A-A to sectional view, film is board-like film.
Figure 12 be Figure 10 fermentor tank along A-A to sectional view, film is a tubular membrane.
Figure 13 is that film and fermentor tank stir shaft are angled, by the structural representation of the understirring type fermentor tank of pipe connection on stir shaft.
The airlift fermentor structural representation of Figure 14 the is built-in film parallel with the fermentor tank longitudinal axis.
Figure 15 is the structural representation of the outside circulation gas lift type fermentor tank of built-in film in the circulating pipe outside.
Embodiment
Below in conjunction with accompanying drawing the utility model is described in further detail.
As shown in Figure 1, this integral type novel fermentation jar comprises tank body 1, film filter 6, and the pipeline 4 that is connected with film filter, film filter 6 places the inside of tank body 1, and the end by pipeline 4 joins, and pipeline 4 the other ends pass tank body 1 wall pass-out.Film filter 6 can be parallel with the longitudinal axis of tank body 1, and number can be symmetrical arranged in jar for two, and film can be board-like film, as shown in Figure 2, also can be tubular membrane, and sectional view as shown in Figure 3.Film filter 6 can also be installed on tank body 1 wall with the fermentor tank longitudinal axis is perpendicular, as shown in Figure 4, the periphery of film filter 6 has been provided with the mounting block 7 of support and fixed action, mounting block 7 with the mode of connection of film can be weld, be threaded, flange is connected, socket connection, fast-assembling clamp connection, also can be the combination of above-mentioned mode of connection.Film filter 6 also can be by on the angled wall that is installed in tank body 1 of pipeline 4.
When fermentor tank is stirred-tank fermenter, film filter 6 can be by parallel vertical or angled being installed on the stir shaft 2 with the fermentor tank longitudinal axis of pipeline 4, as Fig. 7-13, stir shaft 2 communicates with the pipeline 4 of junctional membrane strainer 6 for hollow stir shaft, stir shaft 2 pass-out jars are outer to be connected with other devices, and filtrate flows out fermentor tank by stir shaft.The quantity of film filter 6 every tunic elements and the number of plies of membrane element are according to the size of the size of membrane element and fermentor tank and decide.Each film filter 6 by the fast-assembling clip, weld, be threaded, mode of connection 5 such as flange connection, grafting is connected with pipeline 4.Film filter 6 can be microfiltration membrane or ultra-filtration membrane.Fermentor tank also can be an airlift fermentor, and film filter is installed on the wall of tank body 1 parallel with the fermentor tank longitudinal axis, as Figure 14; Also can be the built-in outside circulation gas lift type film fermentation jar of film in the circulating pipe outside, as Figure 15.Fermentor tank can also be mechanical stirring self-suction fermentor and anaerobic fermentation tank.In a word, the present invention is not limited to the form of fermentor tank, so long as film filter is arranged in the tank body, all belongs to protection scope of the present invention.
During fermentation, film is immersed in the fermented liquid ferments; When needed, start membrane filtration, carry out the membrane sepn operation, the malleation in the fermentor tank is or/and a jar outer negative pressure makes liquid phase material see through film, arrive other outer device of film fermentation jar by pipeline, the cell of suspension and trapped particle are trapped within and continue fermentation in the fermentor tank.Film sees through liquid and enters other devices, further extracts and process for refining.
Claims (10)
1. a novel fermentation jar comprises tank body (1) and pipeline (4), it is characterized in that, the end that described pipeline (4) feeds tank body (1) is connected with film filter (6), and the other end of pipeline (4) is interlinked to the outside with jar.
2. fermentor tank according to claim 1 is characterized in that, described film filter (6) is board-like film or tubular membrane.
3. fermentor tank according to claim 1 is characterized in that, described film filter (6) is ultra-filtration membrane or microfiltration membrane, and the aperture of film is molecular weight 1000 dalton～10 μ m.
4. fermentor tank according to claim 1 is characterized in that, described film filter (6) is at least two.
5. according to any described fermentor tank of claim of claim 1-4, it is characterized in that described film filter (6) is provided with direction and becomes 0-90 ° of angle with the longitudinal axis of jar in tank body (1).
6. fermentor tank according to claim 5 is characterized in that, described film filter (6) is connected on the wall of tank body (1) by pipeline (4).
7. according to the described fermentor tank of claim 5, it is characterized in that, be provided with stir shaft (2) in the described tank body (1) and be installed in stirring arm (3) on the stir shaft (2), the understirring type of described stir shaft (2) for leading to the last stirring-type that gets off from tank deck or leading to up at the bottom of the jar.
8. fermentor tank according to claim 7 is characterized in that, described stir shaft (2) is hollow stir shaft, and film filter (6) is connected with hollow stir shaft by pipeline (4) and leads to outside the jar.
9. fermentor tank according to claim 1 is characterized in that, the material of described film filter (6) is metal or pottery or resin or matrix material.
10. fermentor tank according to claim 2 is characterized in that, the shape of cross section of described tubular membrane is circle or trilateral or water droplet shape or streamlining.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201419099U CN201272805Y (en) | 2008-09-12 | 2008-09-12 | Novel fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201419099U CN201272805Y (en) | 2008-09-12 | 2008-09-12 | Novel fermentation tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201272805Y true CN201272805Y (en) | 2009-07-15 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201419099U Expired - Fee Related CN201272805Y (en) | 2008-09-12 | 2008-09-12 | Novel fermentation tank |
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| Country | Link |
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| CN (1) | CN201272805Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358169B (en) * | 2008-09-12 | 2011-09-07 | 姜泓芳 | Novel fermentation tank |
| CN104017724A (en) * | 2014-05-07 | 2014-09-03 | 广西地源之本肥业有限公司 | Gravity type fermentation and extraction integrated tank and extracting method thereof |
| CN104017725A (en) * | 2014-05-07 | 2014-09-03 | 广西地源之本肥业有限公司 | Stirring gravity type fermentation and extraction integrated tank and extraction method realized by using same |
| CN104031833A (en) * | 2014-05-07 | 2014-09-10 | 广西地源之本肥业有限公司 | Clutch gravity type fermentation and extraction integrated tank and extraction method thereof |
| CN106987527A (en) * | 2017-05-27 | 2017-07-28 | 福建省古田县程久红粬有限公司 | A kind of monascus culture medium and the monascus preparation technology using the culture medium |
| CN108624486A (en) * | 2018-07-05 | 2018-10-09 | 福建师范大学 | A kind of film forming fermentation tank and its application method |
-
2008
- 2008-09-12 CN CNU2008201419099U patent/CN201272805Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358169B (en) * | 2008-09-12 | 2011-09-07 | 姜泓芳 | Novel fermentation tank |
| CN104017724A (en) * | 2014-05-07 | 2014-09-03 | 广西地源之本肥业有限公司 | Gravity type fermentation and extraction integrated tank and extracting method thereof |
| CN104017725A (en) * | 2014-05-07 | 2014-09-03 | 广西地源之本肥业有限公司 | Stirring gravity type fermentation and extraction integrated tank and extraction method realized by using same |
| CN104031833A (en) * | 2014-05-07 | 2014-09-10 | 广西地源之本肥业有限公司 | Clutch gravity type fermentation and extraction integrated tank and extraction method thereof |
| CN106987527A (en) * | 2017-05-27 | 2017-07-28 | 福建省古田县程久红粬有限公司 | A kind of monascus culture medium and the monascus preparation technology using the culture medium |
| CN108624486A (en) * | 2018-07-05 | 2018-10-09 | 福建师范大学 | A kind of film forming fermentation tank and its application method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090715 Termination date: 20110912 |