CN201083712Y - Biosensor and detection device adopting same - Google Patents
Biosensor and detection device adopting same Download PDFInfo
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- CN201083712Y CN201083712Y CNU2007201757300U CN200720175730U CN201083712Y CN 201083712 Y CN201083712 Y CN 201083712Y CN U2007201757300 U CNU2007201757300 U CN U2007201757300U CN 200720175730 U CN200720175730 U CN 200720175730U CN 201083712 Y CN201083712 Y CN 201083712Y
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Abstract
The utility model provides a field effect transistor biosensor, comprising a basal layer and a sealing member which is provided with a plurality of grooved channels, a sample introduction channel and a sample sending channel of samples; each grooved channel is transversely fixed with at least one pair of metal electrodes respectively as a source electrode and a drain electrode of a field effect transistor; a carbon nano-tube is arranged between the metal electrodes which are welded with the carbon nano-tube together; the lower part of the basal layer is provided with a stitch which is connected with the metal electrodes through a conductive adhesive with an insulating layer. The utility model also provides a detection device which adopts the sensor. The biosensor and the detection device adopting the sensor of the utility model have the advantages that: the utility model can be used to detect a plurality of objects, achieves high-flux and high-sensitivity of the detection, has small size, less sample dosage, simple technique and low cost and is suitable for batch production. The detection device has simple and convenient operation, rapid analyzing speed and is easy to achieve online control and detecting automatization.
Description
Technical field
The utility model relates to biology sensor, the pick-up unit of particularly a kind of biology sensor and this sensor of employing.
Background technology
Biology sensor has broad application prospects in fields such as clinical, food, biology, environmental protection tests owing to its excellent selectivity and higher sensitivity.Field effect transistor biology sensor (FET) is a branch very important in the sensor.
The field effect transistor biology sensor is the metal gate electrode that the Biofunctional film replaces isolated gate FET, and the current potential of Biofunctional film and solution or gas interface (or electric charge) changes, and the electric current that is reacted to drain electrode changes, thereby realizes the purpose of detection.Along with the development of micro production technology in recent years, the FET biology sensor is considered to develop the effective means of microbiosensor.
The FET biology sensor of being reported at present utilizes the LB membrane technology mostly, on the FET insulated gate electrode, apply one or more layers Biofunctional materials, or on silicon chip, use the method for making integrated circuit, and several Sensitive Apparatuses are integrated, obtain multi-functional bio-sensing field effect transistor.The preparation of bio-sensitive film is very crucial, the substance fixed technology of multiple biological function of development is such as absorption method at present, cross-linking method, investment, methods such as covalent bond is legal respectively have relative merits, in the substance fixed process of the biological function of reality, usually need as the case may be, the whole bag of tricks is applied in a flexible way, in testing process, conditions such as temperature, pH value of solution value, sample size are also needed in addition strict control.Therefore, the field effect transistor biology sensor is still waiting to improve and development, to improve reliability, stability and the practicality of device.
Since carbon nano-tube in 1991 is found, because its unique 1-dimention nano line structure and electrical properties makes it that immeasurable application prospect be arranged aspect electronic device material.The various countries scientist has carried out the research based on the field effect transistor sensing device of carbon nano-tube in recent years.At present, the biology sensor based on carbon nano-tube also is in the laboratory examination stage.Method for making is carbon nano tube array grows on silicon chip at first, and then structure field effect transistor biology sensor, the sensor object that this method makes up is single, and also there are a lot of problems in the method for preparing carbon nano pipe array at present on chip, because carbon nano tube growth mechanism is complicated, unavoidable impurities (catalyzer, indefinite form carbon etc.) can influence the performance of device greatly in the diameter of carbon pipe, length, the direction of growth and the growth.Simultaneously, this mode can not prepare large-scale high-quality sample in enormous quantities, has seriously restricted its character and applied research.
By above analysis as can be known, at present the problem that exists of field effect transistor biology sensor is also a lot, and helping the biology sensor of marketing and checkout equipment thereof does not have as yet at present and see.
The utility model content
The purpose of this utility model is to provide a kind of high flux and high sensitivity that can detect a plurality of objects and realize detecting, analysis speed is fast, volume is little, sample dosage is few, cost is low, be easy to prepare in enormous quantities and be implemented in line traffic control and detect the field effect transistor biology sensor of robotization, preparation method and adopt the pick-up unit of this sensor.
For achieving the above object, the field effect transistor biology sensor that the utility model provides, comprise basalis and seal, the composition surface of described seal and described basalis is provided with a plurality of downward opening groove type passages, the two ends of described groove type passage vertically are provided with the sample intake passage and the sample output passage of sample respectively, the common fluid passage that forms sample is plugged with the sample introduction pipe respectively and goes out the sample pipe in described sample feeding passage and sample output passage; Be fixed with at least one pair of metal electrode on the described basalis in each described groove type passage respectively respectively as the source electrode and the drain electrode of field effect transistor, be overlapped with carbon nano-tube between the described metal electrode; Described basalis bottom is provided with stitch, and every pair of described metal electrode is connected by its corresponding a pair of described stitch of the conducting resinl that has insulation course respectively.
The utility model field effect transistor biology sensor, wherein said groove type passage is provided with S, P, N, four of B are distributed on the seal.
The utility model field effect transistor biology sensor, wherein said basalis is made of silicon chip and deposition silica coating thereon.
The utility model field effect transistor biology sensor, wherein said seal adopt the dimethyl silicone polymer oligomer to make.
The utility model field effect transistor biology sensor wherein is respectively equipped with a plurality of field effect transistors that are made of metal electrode and carbon nano-tube in each described groove type passage.
The utility model field effect transistor biology sensor, the carbon nano-tube f-CNTs of wherein said carbon nano-tube for modifying by covalent bond or non-covalent bond absorption probe molecule.
The utility model field effect transistor biology sensor, wherein said metal electrode is to being interlaced fork-join shape.
The utility model field effect transistor biology sensor, wherein said groove type passage length is 3-8mm, and width is 0.5-2mm, and the degree of depth is 60-80 μ m, and the diameter of described sample intake passage and sample output passage is respectively 300 μ m-1mm.
For reaching aforementioned purpose, the pick-up unit of the employing the sensor that the utility model provides, comprise field effect transistor biology sensor, sampling system, receiving system and waste liquid outflow system, wherein said sampling system, field effect transistor biology sensor and waste liquid outflow system link to each other successively, described field effect transistor biology sensor links to each other with described receiving system, described sampling system adopts receives the amount of liter syringe pump, and described receiving system adopts electrochemical analyser.
The utility model field effect transistor biology sensor and adopt the advantage of the pick-up unit of this sensor to be: adopt one or a branch of right through the carbon nano-tube connection electrode of modifying, owing to removed impurity through chemical treatment, got rid of or reduced the interference of impurity.Utilize the controllable operating of nano-manipulation instrument realization to carbon nano-tube.And this sensor contains at least four sample channels, each passage can be placed arbitrary number, can detect the field effect transistor of different objects, can be used for detecting a plurality of objects, the high flux and the high sensitivity that detect have been realized, have that volume is little, sample dosage is few, the advantage of easy microminiaturization, technology is simple, and is with low cost, is fit to produce in batches.The pick-up unit that the utility model field effect transistor biology sensor constitutes is simple, convenient, can be widely used in high flux, the detection of aspects such as highly sensitive farming is residual, medicine is residual, gene, accelerated analysis speed, and be easy to be implemented in line traffic control and detect robotization.Be elaborated with reference to accompanying drawing below in conjunction with embodiment, so that the purpose of this utility model, feature and advantage are had deep understanding.
Description of drawings
Fig. 1 is the outside synoptic diagram of the utility model field effect transistor biology sensor;
Fig. 2 adopts the structural representation of the pick-up unit of field effect transistor biology sensor for the utility model;
Fig. 3 is the structure cut-open view of the utility model field effect transistor biology sensor;
Fig. 4 is Fig. 3 vertical view;
Fig. 5 is the local A amplification view of Fig. 4;
Fig. 6 is the side view of Fig. 3.
Embodiment
With embodiment technical scheme is elaborated below.
With reference to Fig. 3, the utility model field effect transistor biology sensor comprises basalis and seal.Basalis is made of silicon chip 21 and deposition silicon dioxide (SiO2) rete 22 thereon.Seal 20 adopts dimethyl silicone polymer oligomer (PDMS) to make.In other embodiment of the utility model field effect transistor biology sensor, also can do basalis by the germanium material.Seal 20 also can adopt polymethyl methacrylate oligomer (PMMA) to make.
With reference to Fig. 2 and Fig. 4, in the utility model field effect transistor biology sensor, seal 20 with the composition surface of basalis on be provided with S, P, N, 1, four groove type passage 1 of four uniform groove type passages of B is respectively applied for treats test sample, the positive control sample, four kinds of sample feedings of negative control sample and blank sample detect.The two ends of groove type passage 1 vertically are provided with the sample intake passage 2 and the sample output passage 3 of sample respectively, form the fluid passage of sample jointly.In the embodiment of the utility model field effect transistor biology sensor, groove type passage 1 length is elected 7mm as, and width is 0.5mm, and the degree of depth is 70 μ m.Sample feeding passage 2 and sample output passage 3 be diameter 500 μ m.In sample feeding passage 2 and sample output passage 3, be plugged with sample introduction pipe 4 respectively and go out sample pipe 5, sample introduction pipe 4 and go out sample pipe 5 and adopt steel pipes or glass (quartz) pipe, internal diameter is 200 μ m or 300 μ m.
With reference to Fig. 3 and Fig. 6, be fixed with at least one pair of respectively (in different embodiment on the basalis in each groove type passage 1, needing to be provided with many at test to metal electrode) metal electrode 7,8 is as source S and drain D, and the carbon nano-tube 9 that employing is modified by covalent bond or non-covalent bond absorption probe molecule between the metal electrode 7,8 is welded to each other together.With reference to Fig. 5, metal electrode is made interlaced fork-join shape to 7,8, to increase the right contact probability of carbon nano-tube connection electrode.F-CNTs carbon nano-tube 9 is fixed on metal electrode on 7,8 by solder technology, and the lead-in wire of metal electrode 7,8 is drawn via the bottom of silicon chip 21 by conducting resinl 11, prevents that liquid from spilling.Basalis is provided with many to stitch 10, and every pair of metal electrode 7,8 links to each other by conducting resinl 11 its corresponding a pair of stitch 10 respectively.
With embodiment preparation process and the method for the utility model based on the field effect transistor biology sensor of carbon nano-tube is described below.
At first, preparation basalis: earlier at 1 * 1cm
2The thick silica coating of deposition 200nm on the silicon chip, the Ti/Au metal electrode of placing interlaced forked type then is right.Preparation dimethyl silicone polymer oligomer seal: use galvanoplastics to make nickel base mould earlier, water the dimethyl silicone polymer oligomer (PDMS) that the two ends that have groove type passage, each groove type passage bottom casting out have vertical sample feeding passage and sample output passage respectively then.The groove type passage length is elected 7mm as, and width is 0.5mm, and the degree of depth is 70 μ m.The diameter of sample feeding passage and sample output passage is 500 μ m.In sample feeding passage and sample output passage, be plugged with the sample introduction pipe respectively and go out the sample pipe, sample introduction pipe and go out the sample pipe and adopt steel pipe or glass (quartz) pipe, internal diameter is 200 μ m or 300 μ m.
Carry out the chemical modification of carbon nano-tube then: utilize covalent bond or the non-covalent bond mode of carboxyl by amido link or ester bond on the carbon nano-tube, antibody or antigen, DNA, fluorescence molecule biomolecule are fixed on the carbon tube-surface, obtain carbon nano-tube f-CNTs through modifying.
To be scattered in through the carbon nano-tube f-CNTs of probe molecule covalency or non-covalent bond chemical modification in aqueous solution or the organic solvent, then hanging drop is added to the surface of electrode pair, vacuum drying, after treating that solvent evaporates finishes, contact condition at indoor observation carbon nano-tube of focused particle beam (FIB) system vacuum and electrode, utilize the nano-manipulation instrument to move nanotube, it is right to make the nanotube two ends connect metal electrode respectively, after put the position, utilize solder technology that carbon nano-tube is fixed on the electrode pair.
Make the lead-in wire of electrode: adopt deep reaction ion etching (DRIE) technology earlier basalis to be run through punching, after having openning hole the oxide isolated processing is done in the hole, in the hole, inject conducting resinl then, the stitch and the conducting resinl of basalis bottom linked together by solder technology.
At last, the using plasma bonding techniques is glued together above-mentioned dimethyl silicone polymer oligomer seal and basalis.With reference to Fig. 2, adopt the pick-up unit of the sensor, comprise field effect transistor biology sensor 31, sampling system 30, receiving system 32 and waste liquid outflow system 33.Wherein sampling system 30, field effect transistor biology sensor 31 and waste liquid outflow system 33 link to each other successively, field effect transistor biology sensor 31 links to each other with receiving system 32, sampling system 30 adopts receives the amount of liter syringe pump, and receiving system 32 adopts electrochemical analysers.Pick-up unit is with fluid syringe pump, field effect transistor biology sensor and electrochemical analyser is effective combines, and can reduce sample size, accelerates analysis speed, has realized On-line Control and robotization.
The following describes the use and the course of work that the utility model adopts the pick-up unit of field effect transistor biology sensor:
Four injection ports of micro-injection pump are linked to each other with four injection ports of sensor respectively, inject detected sample solution simultaneously, contain, do not contain antigenic solution, blank solution with the antibody idiosyncrasy with the antigenic solution of antibody idiosyncrasy.Because the idiosyncrasy between antibody and the antigen causes the carbon nanotube field-effect transistor effect change, can observe the electric relatively variation of leading along with the time of concentration of antigen in the solution by the electric signal analyser.Because the difference of target detection antigenic content in the different solutions, therefore exporting different electricity leads time dependent signal curve, thus can the check and analysis unknown sample in target detection antigen.In like manner, also antigen molecule can be fixed on the carbon nano-tube, detect antibody molecule.
The utility model field-effect transistor biology sensor can be realized unimolecule, high flux, the height of four test sample simultaneously Sensitivity detects, and has that volume is little, sample dosage is few, the advantage of easy microminiaturization, and technology is simple, and is with low cost, is fit in batches Produce, its checkout gear is simple, and is easy to operate, can be used for high flux, the aspects such as highly sensitive farming is residual, medicine is residual, gene Detect.
Claims (10)
1. field effect transistor biology sensor, it is characterized in that: comprise basalis and seal (20), described seal (20) is provided with a downward opening groove type passage (1) at least with the composition surface of described basalis, the two ends of described groove type passage (1) vertically are provided with the sample intake passage (2) and the sample output passage (3) of sample respectively, be plugged with sample introduction pipe (4) respectively and go out sample pipe (5) in described sample feeding passage (2) and sample output passage (3); Be fixed with at least one pair of metal electrode (7,8) on the described basalis in each described groove type passage (1) respectively respectively as the source electrode (S) and the drain electrode (D) of field effect transistor, described metal electrode is overlapped with carbon nano-tube (9) between (7,8); Described basalis bottom is provided with stitch (10), and every pair of described metal electrode (7,8) is connected by its corresponding a pair of described stitch of conducting resinl (11) (10) that has insulation course respectively.
2. field effect transistor biology sensor according to claim 1 is characterized in that: wherein said groove type passage (1) is provided with S, P, and N, four of B are distributed on the seal (20).
3. field effect transistor biology sensor according to claim 1 and 2 is characterized in that: wherein said basalis is made of silicon chip (21) and deposition silicon dioxide (22) rete thereon.
4. field effect transistor biology sensor according to claim 3 is characterized in that: wherein said seal (20) adopts the dimethyl silicone polymer oligomer to make.
5. field effect transistor biology sensor according to claim 4 is characterized in that: wherein said metal electrode (7,8) is welded to each other together with described carbon nano-tube (9),
6. field effect transistor biology sensor according to claim 5 is characterized in that: the carbon nano-tube (f-CNTs) of wherein said carbon nano-tube (9) for modifying by covalent bond or non-covalent bond absorption probe molecule.
7. field effect transistor biology sensor according to claim 6 is characterized in that: wherein said metal electrode (7,8) is interlaced fork-join shape.
8. field effect transistor biology sensor according to claim 7, it is characterized in that: wherein said groove type passage (1) length is 3-8mm, width is 0.5-2mm, and the degree of depth is 60-80 μ m, and the diameter of described sample intake passage (2) and sample output passage (3) is respectively 300 μ m-1mm.
9. adopt claim 1,2,4,5,6,7, the pick-up unit of any sensor in 8, it is characterized in that: comprise field effect transistor biology sensor (31), sampling system (30), receiving system (32) and waste liquid outflow system (33), wherein said sampling system (30), field effect transistor biology sensor (31) links to each other successively with waste liquid outflow system (33), described field effect transistor biology sensor (31) links to each other with described receiving system (32), described sampling system (30) adopts receives the amount of liter syringe pump, and described receiving system (32) adopts electrochemical analyser.
10. adopt the pick-up unit of sensor in the claim 3, it is characterized in that: comprise field effect transistor biology sensor (31), sampling system (30), receiving system (32) and waste liquid outflow system (33), wherein said sampling system (30), field effect transistor biology sensor (31) and waste liquid outflow system (33) link to each other successively, described field effect transistor biology sensor (31) links to each other with described receiving system (32), described sampling system (30) adopts receives the amount of liter syringe pump, and described receiving system (32) adopts electrochemical analyser.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201757300U CN201083712Y (en) | 2007-09-30 | 2007-09-30 | Biosensor and detection device adopting same |
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| CNU2007201757300U CN201083712Y (en) | 2007-09-30 | 2007-09-30 | Biosensor and detection device adopting same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101126735B (en) * | 2007-09-30 | 2010-06-23 | 董益阳 | Field effect transistor biosensor preparation method |
| WO2012142852A1 (en) * | 2011-04-19 | 2012-10-26 | 浙江大学 | High resolution biosensor |
| CN101592626B (en) * | 2009-03-19 | 2013-03-06 | 中国科学院苏州纳米技术与纳米仿生研究所 | Quasi-one-dimensional metal oxide nano-material biosensor and method for manufacturing same |
| CN106706741A (en) * | 2016-11-30 | 2017-05-24 | 陈倩怡 | Preparation method and application of biological sensor using antibody-coupled carbon nanotube as sensitive material |
-
2007
- 2007-09-30 CN CNU2007201757300U patent/CN201083712Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101126735B (en) * | 2007-09-30 | 2010-06-23 | 董益阳 | Field effect transistor biosensor preparation method |
| CN101592626B (en) * | 2009-03-19 | 2013-03-06 | 中国科学院苏州纳米技术与纳米仿生研究所 | Quasi-one-dimensional metal oxide nano-material biosensor and method for manufacturing same |
| WO2012142852A1 (en) * | 2011-04-19 | 2012-10-26 | 浙江大学 | High resolution biosensor |
| CN106706741A (en) * | 2016-11-30 | 2017-05-24 | 陈倩怡 | Preparation method and application of biological sensor using antibody-coupled carbon nanotube as sensitive material |
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| CF01 | Termination of patent right due to non-payment of annual fee |