CN1973614A - Distant crossing method of bamboo and rice - Google Patents

Distant crossing method of bamboo and rice Download PDF

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Publication number
CN1973614A
CN1973614A CN 200610124072 CN200610124072A CN1973614A CN 1973614 A CN1973614 A CN 1973614A CN 200610124072 CN200610124072 CN 200610124072 CN 200610124072 A CN200610124072 A CN 200610124072A CN 1973614 A CN1973614 A CN 1973614A
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China
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bamboo
rice
selection
hybridizing
generation
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CN 200610124072
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Chinese (zh)
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钟章美
黄森伦
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梅州农业学校
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Publication of CN1973614A publication Critical patent/CN1973614A/en

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Abstract

The present invention discloses distant crossing method of bamboo and rice, belongs to the field of rice hybridizing technology, and aims at transferring excellent gene of heterogeneous plant to rice to expand and enrich rice gene bank, create new germplasm source and culturing new rice breed. The method includes the following steps: 1. hybridizing Raoke F4 and green bark bamboo to obtain bamboo rice; 2. obtaining the tillering seedling of the first generation of bamboo rice, treating with colchicina solution and cultivating to obtain second generation bamboo rice F2 seed; 3. hybridizing bamboo rice F2 with Baomei F3 to obtain bamboo Baomei F3; 4. hybridizing bamboo rice F2 with Haikechuan rice to obtain bamboo Kechuan F2 and hybridizing bamboo Kechuan F2 with Guichao 2 to obtain Zhuzazao rice; and 5. hybridizing bamboo Baomei F3 with Zhuzazao rice to obtain Zhudao 966 rice and Zhudao 989 rice.

Description

Bamboo and paddy rice distant crossing method

Technical field

The present invention relates to the paddy rice cross breeding technology, especially a kind of bamboo and paddy rice distant crossing method.

Background technology

The kind of hybrid rice is numerous, at present, yet there are no the relevant report of utilizing bamboo and paddy rice cross breeding.

Summary of the invention

The technical problem to be solved in the present invention is that the excellent genes of allos plant is transferred in the paddy rice, and enlarging and to enrich the paddy gene storehouse, the initiative new germplasm source is cultivated new varieties and a kind of bamboo and paddy rice distant crossing method are provided.

Technical scheme of the present invention is: a kind of bamboo and paddy rice distant crossing method, comprise the steps: (1) adopt the nigihira and the strain system of No. 3 selection cross of section's feelings have mercy on the F4 of section and bambusa texlitis hybridize bamboo rice; (2) the bamboo rice first generation of hybrid prescinds acrial part, and the Mu root is passed the winter, and strips the tillered nursery plant on the first generation of hybrid Mu root, and the colchicine liquid with 0.05% soaks seedling to be handled 3 hours, cleaned implanting and cultivating, two generation seed bamboo rice F2 of results then; (3) bamboo rice F2 gets bamboo Baomei F3 with bag U.S. F3 selection cross of the U.S. 2800 selection cross No. 2 with selecting with bag; (4) get bamboo Kechuan F2 with bamboo rice F2 and Haikechuan selection cross, get Zhuzazao rice with bamboo Kechuan F2 and osmanthus towards 2 selection cross again; (5) get bamboo rice 966, bamboo rice 989 strains with bamboo Baomei F3 and Zhuzazao rice selection cross.

When hybridizing with rich F4 of section and bambusa texlitis in above-mentioned bamboo and the paddy rice distant crossing method, select the colored fringe of the rich F4 of section in full bloom on the same day, killed male method promptly with lukewarm water at 5 o'clock in the morning: 45---46 ℃ Temperature Treatment 3---4 minute clock time, kill male pollen, pat flower fringe grain immediately and extract flower pesticide, bagging behind the arrangement flower fringe; And then the bambusa texlitis pollen that puts out with clean paper bag and loose is cut and is plucked back speed and receive bambusa texlitis pollen and be dispersed on the rich F4 of the section acceptor style to be pollinated, and puts paper bag immediately again, in rascal bamboo full-bloom stage, pollinates continuously 2~3 times.

The hybrid rice new varieties that method of the present invention is cultivated have following advantage:

(1) can open up a new paddy rice cross breeding breeding technique for the crossbreeding paddy rice provides a kind of brand-new cross method.

(2) the paddy rice new lines of the inventive method cultivation, tillering ability is stronger, and plant forms is good, and cane is sturdy, the stem wall thickness, resistant to lodging, well developed root system, drought resistance is strong, resistant to diseases and insects is stronger, and the later stage, ripe look good, about the high 105cm of section, big panicle many grains per panicle, the every fringe of average are more than 140, and many reaches more than 400, ripening rate is more than 90%, and thousand kernel weight 21~23 restrains, 500 kilograms of general per mu yields, reaching more than 600 kilograms of high yield, proterties is stable, no segregation phenomenon; Have higher economic value and application value.

(3) in the bamboo rice new lines of cultivating, contain after testing from the genomic composition of bambusa texlitis by the inventive method.

Description of drawings

Below in conjunction with drawings and Examples the present invention is described in further detail, but does not constitute any limitation of the invention.

Fig. 1 is a schematic flow sheet of the present invention;

Fig. 2 is the gene signal contrast figure of bamboo rice 966 among the present invention, bamboo rice 989 strains and bambusa texlitis.

Embodiment

The selection cross of embodiment 1 bamboo rice 966 and bamboo rice 989

Bamboo of the present invention and paddy rice distant crossing method, its flow process is consulted shown in Figure 1, implements method of the present invention and will achieve success, and solved three big technical barriers: (1) has solved the pollination insemination techniques; (2) solved the sterile problem of hybrid generation; (3) solve breeding cycle length and prevented the problem that the bamboo gene is lost.Below in conjunction with the selection cross process of bamboo rice 966 and bamboo rice 989, details are as follows to each step of the inventive method:

1. grasp the bambusa texlitis flowering habit, select the maternal and suitable male temperature of killing

The rule of blooming of bambusa texlitis has been grasped in logical observation in a few years, and promptly be season of flowers annual 3~June, serving as 4~May to contain to spend period; Under the normal weather condition, the period o'clock is spent for containing in every morning 5~7; The pollen of bambusa texlitis is easy to thirst after surpassing 25 ℃ temperature; Secondly, the bamboo of blooming is then promptly removed bamboo flower fringe after the pollen utilization is intact, and reducing the nutrient consumption of bamboo, control moisture is beneficial to the next year bamboo and continues to bloom again.

2. selecting the rich F4 of section material is maternal and bambusa texlitis hybridization

The rich F4 of section is the strain system that goes out with nigihira and No. 3 selection cross of section's feelings nineteen sixty-eight, and the present inventor once adopted the rich F1 of section, Rao Ke F2, Rao Ke F3 and bambusa texlitis hybridization, all not success before 71 years; In late May, 71, hybridize with rich F4 of section and bambusa texlitis again, and select the colored fringe of the rich F4 of section in full bloom on the same day, killed male pollen in 3~4 minutes with 45~46 ℃ Temperature Treatment at 5 o'clock in the morning, pat flower fringe grain immediately and extract flower pesticide, bagging behind the arrangement flower fringe; And then the blue or green bamboo pollen that puts out with clean paper bag and do not loose, cut and pluck back speed and receive rascal bamboo pollen and be dispersed on the rich F4 of the section acceptor style to be pollinated, put paper bag immediately again, in the bambusa texlitis full-bloom stage, pollination is 2~3 times continuously, evenly is scattered on each column cap of spike of rice grain to guarantee bambusa texlitis pollen.

3. the heredity of bamboo rice F1 performance

Do the hybrid receptor parent with the rich F4 of section and handled 215 colored fringe grains, award and only gather in the crops wherein two fringes totally three hybrids, ripening rate 1.39% behind the bambusa texlitis pollen; In late June, 1971 is received three seeds; dry the back in July 20, sowing was potted plant then; be put in outdoor; when the long 1 cun left and right sides of hybrid seedling, wherein two hybrid seedling are withered naturally, and only depositing a strain, not have dead seedling to move on to little potted plant; take in the room; be placed on window daytime, be placed on night in the open, careful protection.When the hybrid seedling grows 3 when tillering, move on to potted plantly again in the open from little basin, growing way is energetic gradually.Up in mid-January, 1972, only see that a main tiller grows heading, 35 centimetres of its spike lengths that grain is dredged, husk is thick, the fringe grain is many but all sterile.

4. adopt colchicine liquid to handle bamboo rice F1, solve sterile problem

Winter in 1972, the bamboo rice first generation of hybrid prescinds acrial part, and the Mu root is passed the winter, in mid-April, 1973, strip 6 tillered nursery plants on the first generation of hybrid Mu root, colchicine liquid with 0.05% soaks seedling to be handled 3 hours, cleaned implanting and cultivating, on July 26th, 1973 then, hybrid tillered nursery plant after colchicine liquid is handled, have 17 fringes can ear solid, results two generation 162 in seed, ripening rate 8.9~13.3%.Bamboo rice first generation of hybrid plant height 84.6cm, cane is hard, basal part of stem joint is close, the stem wall thickness, blade is smooth, middle arteries and veins white obviously, well developed root system is sturdy, leaf look viridescent has the form of bambusa texlitis at a glance.

5. the heredity of bamboo rice F2 performance

It is very inconsistent to germinate, and makes evening in 1973 and receives 162 of bamboo rice hybrid F2 generations, and seed carries out presoaking and germinating, and the 5th day beginning of vernalization seen germination, the 7th day germination rate be 32.5%, the 11 day be 77.5%, becoming the seedling plant is 92 strains, accounts for 56.8%.

Breeding time is very inconsistent, is 4.3% when making the individual plant that can ear, all forms young fringe after the Beginning of Winter, just heading behind the heavy snow, and 1~February of next year is just ripe, and breeding time was at 180~200 days.Great majority are just gathered in the crops hybrid generation's seed in year the first tenday period of a month in May in mid-July, 1974 to 1975, and be 693 days breeding time the longest, and deviation is big.

The plant forms variation is big, and 51 strains of purple stem account for 55.4%; 41 strains of cyan stem account for 44.6%; Growth potential shows by force, in, weak three classes, be respectively 57.6%, 33.6%, 8.8%.The plant height variation width of cloth is at 60~180cm, and great majority are about 120cm.Length of blade is 14~60cm, and most of blades account for 73.9% at 14~29cm.Master pulse of blade is in the majority, but long two master pulses, three master pulses and not obvious master pulse are also arranged, and also has tubular or a slice leaf upper half part to become two to split shape, or the plant type collection that two leaves grow simultaneously has 19 strains, account for 20.65%, scattered have 73 strains, accounts for 79.35%.The grain form has round shaped grain, long grain, rice end purple, redness, long awns, short awns is arranged and do not have awns etc.

Between second generation colony, that has outstanding performance has: growth vigor is big, well developed root system, and tillering ability is strong, and the leafiness number is many, and blade is firmly directly thick, cane is hard, and the stem wall thickness is close anti-between stipes, the feature of strong stress resistance etc.

6. the bamboo rice hybrid utilizes and bamboo rice 966, bamboo rice 989 hereditary and selections

For the bamboo gene of guaranteeing Rao Ke F4/ bambusa texlitis filial generation is not lost, early made the filial generation of just adopting bamboo rice since 74 years, even the complex of two kinds of bamboo rice carries out backcrossing repeatedly, reestablishes diplomatic relations, made until 91 year evening and adopt bamboo Baomei F3/ Zhuzazao rice, therefore make in bamboo rice 966, bamboo rice 989 genes and still remain with the genomic composition of bambusa texlitis so far, process is as follows:

Early made in (1) 1974 year, and utilized bamboo rice F2 and the cross-breeding of the beautiful F3 material of bag, wrap beautiful F3 and be bag and select No. 2 with the U.S. 2800 hybridization back seed selections, hybridize with bamboo rice F2 then, its offspring experiences time systematic breeding more than 5 years and names and be bamboo Baomei.

Make (2) 1974 year evening, utilize bamboo rice F2 and the breeding of Haikechuan paddy rice cross breeding, and its offspring names through seed selection in 3 years and is bamboo Kechuan.

Make (3) 1977 year evening, utilizes bamboo Kechuan F 2With osmanthus towards No. 2 hybridization, name through 5 years systematic breedings and to be Zhuzazao rice.

Make (4) 1991 year evening, in order to strengthen the effect of bamboo gene, utilizes bamboo Baomei F3 and Zhuzazao rice to hybridize again, when making 8 hybrids of results, early made sowing in 1992, and 8 hybrids all survive.Making selected 2 individual plants in evening in 1992 is, its performance: tillering ability is strong, and cane is sturdy, the stem wall thickness, well developed root system, big panicle many grains per panicle, solid normal, the plant forms ideal, comprehensive proterties two individual plants are more stable, make morning in 1993 selected 44 individual plants, 11 comprehensive proterties unanimities of individual plant wherein, mixed seeding selects 108 individual plant systems again, is divided into six types and carries out systematic breeding.

It is more stable to go into roguing system each proterties of performance after (5) 1994 years, again through cultivating seed selection, is to name to be bamboo rice 966 in strain in 1996 the 6th; No. 9 strain in 1998 system names the target that has reached seed selection into the characteristic of 989, two strains of bamboo rice, and progressively is used for producing.

7. feature of bamboo rice 966, bamboo rice 989 and output performance

Bamboo rice 966, bamboo rice 989 in 1996,1998 respectively in Meizhou City Jiangling, Guangdong Province, part small towns, Mei County plants experimentally, it is existing to take temperature through the district in 2 years of the land for growing field crops illness that has not attacked the vital organs of the human body and the Meizhou City institute of agricultural sciences, bamboo rice 966, bamboo rice 989 all belong to the temperature sensitive type kind, early make and plant about 126 days and the 132 days time of infertility, make evening and plant about 109 days and the 115 days time of infertility.Tillering ability is stronger, and plant forms is good, and cane is sturdy, the stem wall thickness, resistant to lodging, well developed root system, drought resistance is strong, and resistant to diseases and insects is stronger, and the later stage, ripe look good, about the high 105cm of section, big panicle many grains per panicle, general every total grain panicle is several more than 140, many reaches more than 400, and ripening rate is more than 90%, and thousand kernel weight 21~23 restrains, 500 kilograms of general per mu yields, the reaching more than 600 kilograms of high yield, outward appearance rice is of fine quality.

Embodiment 2

Genetic test

Detection method: adopt the Southern hybrid method, with japonica rice variety " Japan is fine " and the negative contrast of rice variety " 93-11 ", the positive contrast of original male parent " bambusa texlitis " with the bamboo rice, extract " Japan is fine " respectively, " 93-11 ", " bamboo rice 966 ", the genomic DNA of " bamboo rice 989 " and " bambusa texlitis ", five parts of DNA samples are carried out complete degestion with EcoR I and HindIII respectively, after cutting, enzyme carries out electrophoresis, change film and crosslinked, with " Japan fine " of complete degestion and the genomic DNA of " 93-11 " blockade (prehybridization), " bambusa texlitis " genomic DNA with the tagging complete degestion is hybridized as probe, washes film at last, compressing tablet, develop a film.

Testing result: the Southern results of hybridization is seen shown in Figure 2, as can be seen from Figure 2, the very strong and negative control signal of positive control signal a little less than, bamboo rice fret signal obviously is better than negative control, can infer the genomic composition that contains in the genome of " bamboo rice 966 " and " bamboo rice 989 " from " bambusa texlitis "; Among the figure: J: fine, the I:93-11,6 of Japan: bamboo rice 966,8: bamboo rice 989, B: bambusa texlitis.

Claims (2)

1. bamboo and paddy rice distant crossing method, it is characterized in that comprising the steps: (1) adopt the nigihira and the strain system of No. 3 selection cross of section's feelings have mercy on the F4 of section and bambusa texlitis hybridize bamboo rice; (2) the bamboo rice first generation of hybrid prescinds acrial part, and the Mu root is passed the winter, and strips the tillered nursery plant on the first generation of hybrid Mu root, and the colchicine liquid with 0.05% soaks seedling to be handled 3 hours, cleaned implanting and cultivating, two generation seed bamboo rice F2 of results then; (3) bamboo rice F2 gets bamboo Baomei F3 with bag U.S. F3 selection cross of the U.S. 2800 selection cross No. 2 with selecting with bag; (4) get bamboo Kechuan F2 with bamboo rice F2 and Haikechuan selection cross, get Zhuzazao rice with bamboo Kechuan F2 and osmanthus towards 2 selection cross again; (5) get bamboo rice 966, bamboo rice 989 strains with bamboo Baomei F3 and Zhuzazao rice selection cross.
2. bamboo according to claim 1 and paddy rice distant crossing method, when it is characterized in that hybridizing with rich F4 of section and bambusa texlitis, select the colored fringe of the rich F4 of section in full bloom on the same day, kill male method with conventional lukewarm water at 5 o'clock in the morning and kill male pollen, pat flower fringe grain immediately and extract flower pesticide, bagging behind the arrangement flower fringe; And then the bambusa texlitis pollen that puts out with clean paper bag and loose is cut and is plucked back speed and receive rascal bamboo pollen and be dispersed on the rich F4 of the section acceptor style to be pollinated, and puts paper bag immediately again, in rascal bamboo full-bloom stage, pollinates continuously 2~3 times.
CN 200610124072 2006-12-07 2006-12-07 Distant crossing method of bamboo and rice CN1973614A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039352A (en) * 2011-10-17 2013-04-17 钟献铭 Method for cross breeding of paddy and bamboo
CN107306787A (en) * 2017-07-26 2017-11-03 中国林业科学研究院亚热带林业研究所 A kind of method of bamboo hybridization pollination

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039352A (en) * 2011-10-17 2013-04-17 钟献铭 Method for cross breeding of paddy and bamboo
CN107306787A (en) * 2017-07-26 2017-11-03 中国林业科学研究院亚热带林业研究所 A kind of method of bamboo hybridization pollination

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