CN1866008A - Assembled type multifunctional sensor device and preparation method thereof - Google Patents
Assembled type multifunctional sensor device and preparation method thereof Download PDFInfo
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- CN1866008A CN1866008A CN 200610040216 CN200610040216A CN1866008A CN 1866008 A CN1866008 A CN 1866008A CN 200610040216 CN200610040216 CN 200610040216 CN 200610040216 A CN200610040216 A CN 200610040216A CN 1866008 A CN1866008 A CN 1866008A
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Abstract
The disclosed assembly-type multifunction sensor fit for large-scale production comprises: a mobile sensing unit (1) arranged on a sensing energy-conversion platform (2), a control device (3) on side of (3) better on bottom, and an auxiliary unit. This invention also discloses the preparation method.
Description
Technical field
The present invention relates to a kind of sensor and preparation method thereof, especially a kind of sensor that is used for synchronous detection biology in enormous quantities, chemical substance, specifically a kind of assembled type multifunctional sensor device based on functional particulate and preparation method thereof.
Background technology
Can detect simultaneously multiple material the multifunctional sensing device since its volume little, contain much information, functional, easy to use, reliability is high, be developing direction biological, the chemical sensitisation device, be subjected to showing great attention to of whole world researcher and sensor supplier.At present, biological, chemical material multifunctional sensing device mainly concentrates on array sensing chip and the micro-fluid chip.The array sensing chip is mainly by detecting multiple biology, chemical substance with example reaction after preparing sensing responsive material array in that certain carrier surface is synthetic by point sample, original position.It is low that the subject matter that this method exists is diffusion reaction time length, the sensing responsive storeroom may exist mutual pollution problems and usable range to be confined to nucleic acid and protein material and point sample method density, and the high problem of original position synthetic technology cost.The major advantage of this method is that flux is big.Micro-fluid chip also cry micro-fluidic chip it by the microchannel network function in whole laboratory is comprised sampling, dilutes, adds reagent, reaction, separation, detection etc. and be integrated on the reused microchip.The advantage of micro-fluid chip is applied widely.But exploitation that its subject matter comprises that flux is low, gordian technique is still needed etc. limits its widespread use.
Summary of the invention
The purpose of this invention is to provide a kind of array-type sensor spare that is suitable for standardized production in enormous quantities and preparation method thereof.
Technical scheme of the present invention is:
A kind of assembled type multifunctional sensor device based on functional particulate, comprise some identical or different portable sensing function unit (1) that the sensing responsive material can detect specific detected material that have, sensing transducing platform (2), frame (4), cover plate (5) and actuation means (3), it is characterized in that the surface is connected with the portable sensing function unit (1) of sensing responsive material can be under the energy field effect of actuation means (3), by sensing transducing platform, cover plate, (6) and detected material effect in the sensing transducing space that frame constitutes, also can under the effect of the energy field of actuation means, carry out separating substances, also can be under the effect of the energy field of actuation means and be positioned to be used on the sensing transducing platform (2) to detect.
Detected material is being handled the back or is directly being introduced sensing transducing space through the external world or auxiliary unit, under the actuation means effect, mix, act on portable sensing function unit (1), other reagent, then under actuation means (3) effect, the sensing function cell location detects under the situation of detected material, other reagent existence or separation on sensing transducing platform (2).
Portable sensing function unit 1 is can be to the functional unit of certain biology, the responsive response of chemical substance.Portable sensing function unit is a kind of functional particulate, and pattern, material are optional, preferred tumbler structure, and size is between 20 nanometers to 1 centimetre.So-called tumbler structure is meant that the bottom of structure is an arc, and center of gravity is in the structure of bottom, and this class formation can guarantee that sensing function unit 1 can freely stand in the plane, and is perhaps fixing closely in the hole, hole 4 of sensing transducing platform 2.To guarantee that it can be fixed in the hole, hole of flue of a kang formula sensing transducing platform easily or disperses freely to switch between motion outside the fixing and hole, hole in the hole, hole.
Portable sensing function unit contains sensing responsive material and support structure material at least.Sensing responsive material and support structure material can be that different materials also can be with a kind of material.Sensing responsive material part can be that the smooth structure that also can be coarse or porous structure but preferably adopt high-specific surface area is to improve sensing capabilities.Functional particulate in same sensor preferably is connected with the surface size unanimity of sensing responsive material or has the functional particulate of comparability surface area.For improve portable sensing function unit can inside, handling sensing function unit or the surface preferably comprise magnetic, electricity, sound, heat or electromagnetic response component.Portable sensing function unit can carry out mark when being used to detect also can not carry out mark, but preferably carries out mark.
Sensing transducing platform 2 is the functional units that can carry the certain face structure of having of sensing function unit 1.Its effect shows that mainly carrying sensing function unit and conduction external energy field orientation sensing functional unit are on the sensing transducing platform 1 and guarantee that sensing function unit 1 can be positioned on the sensing transducing platform 2 and externally disperse under the effect of energy field, switches between motion.Sensing transducing platform can be actual platform and also can be virtual platform.When sensing transducing platform is actual platform, the face structure of sensing transducing platform can be the optional face structure of plane, curved surface or pattern and preferably have planar structure with the flue of a kang array structure of sensing function units match, footpath, the hole of flue of a kang size is between 20 nanometers to 1 centimetre, and its surface can be loaded with the P.e.c. that links to each other with each hole, hole as required.Sensing transducing platform can be the single or multiple lift structure, but preferred sandwich construction, the upper strata is to be loaded with hole hole array and is the good conductor of energy field in the thin energy field poor conductor lower floor of hole hole sole, perhaps the upper strata is that the energy field good conductor lower floor that is loaded with hole hole array is except then being energy field poor conductor material with corresponding part at the bottom of the hole, hole for the other parts energy field good conductor, to guarantee the energy field useful effect, make sensing transducing unit can be fixed in sensing transducing platform effectively or in sensing transducing space, disperse in sensing transducing unit, the convenient switching between motion.It also can be that the sensing function unit can switch between fixing on the sensing transducing platform and dispersion, motion that the sensing function unit can be fixed on the sensing transducing platform.The shape of sensing transducing platform comprises optional planar structures such as ribbon, rectangle, also can be band on rotating disk, micro-fluid chip shape, array chip shape, pen type etc.When sensing detection utilizes galvanochemistry, resistance, inductance, electric capacity etc. to relate to the use of electricity, also be printed on the P.e.c. that is communicated with external power source on the sensing transducing platform 2.When sensing transducing platform was virtual platform, actuation means can be fixed on portable sensing transducing unit on one or more plane or curved surface that can detect by energy field.
Sensing transducing platform can have the diaphragm structure of hole, extraordinary hole array by offset printing-etching combined techniques, template stamped method, injection molding preparation.Template stamped method described here refers to the template that will be loaded with the thrust array corresponding with extraordinary pit structure and is pressed into owing to former thereby softening material surfaces such as fusion, dissolvings, can obtain hole, extraordinary hole array after the softener material hardening is solidified.The template that is loaded with the thrust array corresponding with extraordinary pit structure can be by offset printing-etching combined techniques or the preparation of other method.Can comprise as required that above the diaphragm structure that is printed on hole, extraordinary hole array processing such as film sedimentation, circuit printing, offset printing-etching make this structure and sensing function unit compatibility and suitable relevant detection means detect then and should print P.e.c. on the surface with galvanochemistry as needs.Be to have good energy field ducting capacity and can combine good material below the diaphragm structure that is printed on hole, extraordinary hole array, as magnetic field being had good ducting capacity and combining good macromolecular material with hole, hole array diaphragm structure macromolecular material with the diaphragm structure that is printed on hole, extraordinary hole array.Auxiliary unit comprises that micropump etc. can also be fixed on the sensing transducing platform except can being fixed on sensing transducing space top cover.
Control mode based on the actuation means of the assembled type multifunctional sensor device of functional particulate comprises mixing, location, separation and temperature control.Mixed method has mechanical raking, mechanical mixture, magnetic agitation, ultrasonic mixing, rotating electric field to mix, and localization method has gravitational orientation, location, magnetic field, electric field location, centrifugal force location, centripetal force location, mechanical positioning.Separation method has that filtration, absorption, mechanical fixation separate, centrifugal, extraction, electrophoresis, chromatography.When adopting magnetic force to control, portable sensing function unit preferably comprises magnetic component.Actuation means can be combined with assembled type multifunctional sensor device, also can separate with assembled type multifunctional sensor device.Mechanical actuation means based on the assembled type multifunctional sensor device of functional particulate can have only one, also can have a plurality of.
Assembled type multifunctional sensor device has cover plate, frame, and cover plate (5), frame are formed transducing space (6) with sensing transducing platform (2).Sensing transducing space 6 is can hold the sensing function unit 1 that leaves sensing transducing platform 2, detect the space with reagent and test sample.It is that sensing transducing space 6 can have only a chamber also can comprise a plurality of chambers that sensing transducing space can be separated into completely separated or partially separated structural unit by separation structure.When surpassing a chamber, can circulate fully between chamber, can also not communicate mutually by valve controlling flow is logical.Different types of sensing function unit chamber that can coexist also can be at different chamber, and reagent between chamber, solution can free flow, controlled circulations or can not be circulated.When needs are separated different types of sensing transducing unit 1, also have dam formula structure dividing strip to make the distance at top in the top of dam formula dividing strip and sensing transducing space 6 on the sensing transducing platform 2 less than the size of sensing transducing unit 1.Can have injection orifice (501) on the cover plate (5).In the outside in sensing transducing space (6) auxiliary unit (7) is installed.Auxiliary unit 7 has the structural unit of subsidiary function such as sample injecting unit, promotes solution round-robin micropump, temperature regulating device etc. for some.Injecting unit is fixed on the top cover 5 in sensing transducing space 6 usually or side micropump etc. then is fixed on the sensing transducing platform 2 usually.Auxiliary unit can realize sampling, purifies, in the separation, enrichment, reagent interpolation, reaction, cleaning, discharging, temperature control, fluid FLOW CONTROL, pressure control function, data acquisition, analysis, output one or more.
Can being realized of said modules formula multifunctional sensing device by following preparation method:
A, at first will as organic material, inorganic material or the inorganic-organic composite material of preparation material by injection molding, microballoon polymerization synthetic method, patterning method, offset printing-etching combined techniques or fluid do not have the mould solidification method simultaneously prepared in batches obtain the idiosome particle;
Secondly b, the surface is not had the idiosome particle that the connects functional group method by chemistry or physics and make its surface form connection functional group; The surface itself is had the idiosome particle that connects functional group, then directly carry out next step operation;
C, then the method by chemistry or physics above-mentioned have connect functional group the idiosome particle surface fixedly the sensing responsive material promptly make the sensing function unit;
D, comprise flat board by choosing suitable plate again, have the sensing transducing platform of the optional panel in the plane in the hole, array hole that the size of size and corresponding sensing function unit is complementary or curved surface shape sheet material or pattern, also can choose and to be empty sensing transducing platform by the dummy rules face that sensing transducing cell location be controlled in the external energy field as reality;
E, at last with the above-mentioned sensing function unit that makes, sensing transducing platform and cover plate, frame, auxiliary unit assembling, promptly get assembled type multifunctional sensor device of the present invention with actuation means combination assembling or that separate then.
Sensing function unit among the preparation method of assembled type multifunctional sensor device has sensing mark, this sensing mark or when the idiosome preparation of granules, make, or the increase when fixing sensing responsive material, or fixedly increasing behind the sensing responsive material.The method of mark comprises that single or multiple fluorescent material carries out mark, single or multiple color material (comprises dyestuff, non-ferrous metal, the schemochrome material comprises interference, diffraction, photon crystal structure look material) carries out mark, single or multiple luminescent material carries out mark, simple or complex geometric shapes comprises that the geometry sizes mode carries out mark, utilize the position to carry out mark, utilize element to comprise that radioactive isotope carries out mark, conductivity carries out mark, magnetic property carries out mark, bar code is carried out mark, and two kinds of above labeling method or two or more compound token methods are carried out mark.
The assembling of assembled type multifunctional sensor device (being about to structures such as cover plate, frame, sensing transducing platform, control device connects) can method such as be sticked, inlay by tackifier, heat and be carried out.Can sensing transducing unit 1 be directed into the surface of sensing transducing platform 2 during concrete the use by methods such as point sample method, fluid transport methods to the assembling of sensing transducing unit 1 on sensing transducing platform 2, realize fixing by the control device 3 of sensing transducing platform 2 belows then, and sensing transducing unit 1 leave sensing transducing platform 2 disperse, move also can by above the sensing transducing platform 2 or below control device 3 implement.
Assembled type multifunctional sensor device detects as uv-vis spectra by electromagnetic signal, infrared spectrum, atomic spectrum, luminous detection, waveguide detects, unit such as surface is sharp from spectral technique, white light interference spectrum etc., electrical property detects and leads detection as electricity, capacitance detecting, Electrochemical Detection, piezoelectric detection technology etc., magnetic property detects as magnetic resonance detection, magnetic coercive force detection etc., power Performance Detection such as gravimetric analysis, pressure analysis etc., acoustical behavior detects and detects as audio frequency, sound width of cloth detection etc., thermal behavior detects as thermometric analysis, differential thermal analysis (DTA), thermal conductance detections etc., imaging detect i.e. two dimension or the above multidimensional physics of two dimension, chemistry, one or more methods in the methods such as the detection of biological parameter such as microscopic examination detect.During use, can also can be to the detection of assembled type multifunctional sensor device by unmarked detection by marker detection.
As mentioned above, sensing transducing of the present invention unit, sensing transducing platform and other auxiliary unit can be concentrated in batches by the standard component formula and produce, and its sensing transducing cell size is between between 20 nanometers to 1 centimetre; Can be earlier during manufacturing by injection molding, microballoon polymerization synthetic method, patterning method, offset printing-etching combined techniques or there is not the sensing function unit embryo that template fluid solidifies method is prepared into extraordinary 3D shape, thereby functional group then by existing or the tool linkage function introduced such as hydroxyl, carboxyl, amido, aldehyde radical etc. connect sensing responsive material preparation sensing transducing unit.Described injection molding is meant that the fluid attitude material that can solidify injects and solidifies in the mould of given shape and obtain the method for given shape particle.The material of injection molding comprises under the normal temperature normality for solid-state and also to comprise and can become solid-state material by chemical reaction owing to dissolving, fusion become the material of fluid state.The microballoon synthetic method be meant utilize surface tension with fluid attitude material by polymerization, solidify, method that solvent evaporates, curing reaction form ball material.Patterning method is meant by solid-state material being cut into the method that specific 3D shape obtains the given shape particle.Cutting method comprises machine cuts, chemical corrosion, fluid cutting.No template fluid solidifies method is meant that the fluid that can solidify solidifies and obtains the method for given shape particle under the certain condition of no template.Fluid described in above-mentioned all methods can be that gas, liquid, supercritical fluid, plasma, liquid particles are contained in carrying object, solid granulates is contained in carrying object.The functional group that wherein introduces the tool linkage function introduces the functional group of tool linkage function or introduces the functional group of tool linkage function or the functional group that introduces the tool linkage function by methods such as vacuum coating, magnetron sputtering plating, vapour depositions behind the surface deposition film of the sensing transducing unit of extraordinary 3D shape by the plasma gas reaction or by chemical reaction by coupling reagent such as silane coupling agent, titanate coupling agent, aluminate coupling agent etc.Described sensing responsive material comprises cell sensitive material, bacterium sensitive material, viral sensitive material, albumen sensitive material, nucleic acid sensitive material, ion-sensitive material, micromolecule sensitive material, is loaded with the carrier of sensing responsive material as introducing the macromolecular material of sensing responsive functional group or functional group's group.Described sensing function unit is used for the sensing purpose by assembling on sensing transducing platform.The sensing function unit can be fixed on the sensing transducing platform by the external energy field, also can comprise that with other material separates in detected sample by the external energy field, can also be dispersed in the sensing transducing space by the external energy field, these processes can repeat or circulate to carry out.Described external energy field can be mechanicals efforts, magnetic field, electric field, sound field, electromagnetic field.The preferred tumbler structure in described sensing transducing unit is that the bottom is an arc, and the shape on top then can be chosen wantonly, and sensing transducing unit center of gravity guarantees that the sensing function unit can stand on the plane.
The sensing function unit can be fixed on Wu Keng hole or the cratered sensing transducing platform by energy field.For sensing transducing platform that the flue of a kang is arranged and the bottom that need dock with the flue of a kang on the sensing transducing platform is the sensing function unit of arc, when not being spherical as the sensing function unit, the sensing function unit combines the cross section of the cross section near coal-mine place more than or equal to sensing function unit, near coal-mine below and junction, sensing transducing platform hole with sensing transducing platform, when sensing function unit when being spherical, the radian of sensing function unit smaller or equal to the radian in sensing transducing platform hole with the sensing responsive material of guaranteeing sensing transducing unit sensing transducing platform in conjunction with the hole outside.
Sensing function unit 1, sensing transducing platform 2, sensing transducing space 6 and auxiliary unit 7 particularly sensing transducing unit 1 all can be by standard component formula batch centralized production.
The material of making the sensing function unit comprises organic material such as organic macromolecular material, organic small molecule material, inorganic material such as inorganic ceramic material, inorganic metal material and inorganic-organic composite material.
The assembling of multiple sensing function unit on sensing transducing platform can be controlled method by point sample method, machinery, fluid is written into method and is written into assembling.
The material that is detected comprises miniature organism bodies such as cell, bacterium, virus, biology, chemical micromolecule, gases such as the big molecule of biological chemistries such as albumen, nucleic acid, ion, sugar, amino acid.
Beneficial effect of the present invention:
The notion that the present invention produces standard component in batches is first introduced multifunctional bio chemical sensitisation device field, produces sensing transducing unit, sensing transducing platform and other auxiliary unit in batches by the standard component formula and produces multifunctional bio, chemical sensitisation device by assembling then.Can effectively disperse by the control of external energy field, move and the narrow application range, the sensing responsive material that fixedly have solved array biochip exists and pollute mutually and shortcoming such as diffusion reaction time length and the micro-fluid chip flux is low, prepare complicated problems because the multifunctional sensing device output of producing by this technology is big, cost is low, be easy to the multifunctional sensing device sensing transducing unit after the microminiaturized and assembling, can be efficient thereby can obtain, the high-performance multifunctional sensing device of the multiple biology of synchronous detection, chemical substance with cheap price.
The advantage applies of concentrated batch process assembly type sensing of the present invention is aspect following two:
The one, what use when making the idiosome particle of sensing function unit is the method for idiosome particle of specification of the same race of can be mass-produced, and the idiosome particle that the method that also can adopt chemistry or physics when the idiosome particle manufactured the sensing function unit that has the sensing responsive material obtains above-mentioned batch process is produced the sensing function unit of different sensing responsive materials again with form in batches, thereby available a kind of method obtains large batch of sensing function of the same race unit simultaneously, different combinations can be produced different sensing transducing unit in enormous quantities, therefore can reduce the manufacturing cost of sensing transducing unit greatly.
The 2nd, with sensing function unit and the assembling of sensing transducing platform the time, on same platform, can assemble the sensing function unit of multiple different sensing responsive materials simultaneously, make that a sensor can the different object of while batch detection.
Description of drawings
Fig. 1 is the structural representation of senser element of the present invention.
Embodiment
The present invention is further illustrated below in conjunction with drawings and Examples.
As shown in Figure 1.
A kind of assembled type multifunctional sensor device, comprise sensing function unit 1, sensing transducing platform 2, actuation means 3, frame 4, cover plate 5, auxiliary unit 7, the array that sensing function unit 1 is installed on the sensing transducing platform 2 is cheated in the hole 8, and magnetic force generating means 3 is positioned at the below of sensing transducing platform 2; Hole 8, array hole on the profile of sensing function unit 1 and the sensing transducing platform 2 matches, its internal package particle 101 that is magnetic, its surface is connected with the sensing responsive material, the size of sensing function unit 1 is between 20 nanometers to 1 centimetre, on the top of sensing function unit 1 cover plate 5 that has injection orifice 501 is installed, cover plate 5 is formed a transducing space 6 with sensing transducing platform 2.Sensing transducing platform 2 is provided with several dam shape structures that is divided into the district.In the bottom of sensing transducing platform 2, the top of magnetic force generating means 3 is provided with the magnetic conductance plate that matches with sensing transducing platform 2.The outside at sensing transducing platform 2 is equipped with auxiliary unit 7; Auxiliary unit 7 can be miniature circulating pump.
It is made up of four parts usually.Its bottom is preferably arcuate structure to guarantee it and can be fixed in the hole, hole of flue of a kang formula sensing transducing platform easily or to disperse freely to switch between motion outside the fixing and hole, hole in the hole, hole.Its middle part is the optional supporting construction material of geometric configuration, be used to connect bottom and top, its top is the quick material membrane of sensing, contain sensing responsive component, marker components, include in the supporting construction material and be convenient to whole sensing function singly location, fixing magnetic-particle on sensing transducing platform 2, the sensing responsive material membrane can be smooth also can be coarse or porous structure.The sensing responsive material membrane can be arc also can be plane.When being plane, sensing responsive film can select plane geometric shape arbitrarily for use.For non-arc sensing transducing unit 1, sensing transducing unit 1 is fixed on the cross section of hole, hole 4 oral areas of sensing transducing platform 2 smaller or equal to the cross section of sensing transducing unit 1 top sensing responsive material membrane, to guarantee that sensing responsive material membrane for non-arc sensing transducing unit 1 is outside the hole, hole of sensing transducing platform 2.For arc sensing transducing unit 1, the radian of sensing transducing unit 1 can be fixed in the hole, hole of sensing transducing platform 24 smaller or equal to the radian in hole 4, sensing transducing platform 2 hole to guarantee arc sensing transducing unit 1.
Sensing transducing platform 2 is the functional units that can carry sensing transducing unit 1.Its effect mainly show selectivity conduction external energy field fixedly the sensing function unit in the hole, hole 4 of sensing transducing platform 1 in and guarantee sensing transducing unit 1 can be the hole, hole 4 of being fixed in sensing transducing platform 2 densely in and externally dispersion under the effect of energy field, move between switching.Sensing transducing platform 2 is main by forming with hole, hole 4 arrays of sensing function unit 1 driving fit and the magnetic force generating means 3 of below thereof.Magnetic force generating means 3 comprises can be reversible or irreversibly fixing or disperse the magnetic field, electric field, sound field, electromagnetic field etc. of sensing transducing unit.When sensing detection utilizes galvanochemistry, resistance, inductance, electric capacity etc. to relate to the use of electricity, also be printed on the P.e.c. that is communicated with external power source on the sensing transducing platform 2.
Details are as follows:
Can at first be prepared into extraordinary 3D shape for sensing transducing unit by impression injection molding (being similar to the method for casting), microballoon polymerization synthetic method, offset printing-etching combined techniques, patterning method, no template fluid solidifies method, obtain the idiosome particle that inside includes magnetic-particle, if containing, the top layer of preparation material connects functional group, as hydroxyl, carboxyl, amido, aldehyde radical etc., the functional group that then need not introduce the tool linkage function; If not then need to comprise that silane coupling agent, titanate coupling agent, aluminate coupling agent etc. are introduced the functional group of tool linkage functions or by plasma gas reaction or the functional group that introduces the tool linkage function by chemical reaction by coupling reagent; As required can be if its surface is not suitable for fixedly connected functional group in the one side of fixing sensing responsive material by methods such as vacuum coating, magnetron sputtering plating, vapour depositions on the surface of the sensing transducing unit of extraordinary 3D shape by gas phase or liquid deposition film, and then introduce and connect functional group.For containing or having introduced fixedly sensing responsive material of method that the top layer that connects functional group then can be by chemical Covalent Immobilization or physisorption.The sensing responsive material comprises the material of albumen, nucleic acid, ion, micromolecule sensitivity and is loaded with the macromolecular material of the carrier of sensing responsive material as introducing sensing responsive functional group or functional group's group.Here the impression injection molding of being said refers to melted material, macromolecule is organic or inorganic material solution, can react the organic and inorganic small molecule material solution that becomes macromolecular material and inject the mould that can finally form required sensing transducing unit three-dimensional shape, the elimination of solvent in the organic or inorganic material solution by cooling, the macromolecule of fused solution, can form the germule of extraordinary sensing transducing unit by the elimination that reaction becomes the polymerization of organic and inorganic small molecule material solution of macromolecular material and solvent.The microballoon synthetic method refer to by in solvent or gas, utilize surface tension make macromolecular material solution or can form sphere by solution or the melted material that polymerization, other chemical reaction form macromolecular material, utilize then solvent in the macromolecule material solution elimination, can form the polymerization of solution of macromolecular material and the elimination of solvent by polymerization, other chemical reaction, the cooling of melted material obtains microballoon.Offset printing-etching method refers to by lithographic printing and at first is printed on special selected pattern then by selecting suitable lithographic technique to etch special three-dimensional appearance at certain material surface, can repeat offset printing-etching process if desired so that obtain final required three-dimensional appearance.Material therefor can be that fluent material also can be the potpourri of fluid and solid material or be the particle of solid-state material fully completely in impression injection molding and the microballoon synthetic method.
During concrete enforcement, sensing transducing platform also can be plate armature, and its surface can not process or process very shallow hole, aforesaid hole, the effect in the magnetic field that fixedly places one's entire reliance upon of sensing transducing unit.
Labeling method in the marker detection comprises that single or multiple fluorescent material carries out mark, single or multiple color material (comprising that dyestuff, non-ferrous metal, schemochrome material comprise interference, diffraction structure look material) and carries out mark, single or multiple luminescent material and carry out mark, simple or complex geometric shapes and comprise that the mode of geometric configuration, size carries out mark, utilizes the position to carry out mark, utilizes element to comprise that radioactive isotope carries out mark.Mark can adopt a kind of labeling method, also can adopt two kinds or two or more labeling methods.That to comprise that absorption spectrum, fluorescence spectrum, element detection, Electrochemical Detection, luminous detection, piezoelectric detection, thermal conductance detect, electricity is led detection, optical microscope, fluorescent microscope, infrared microscope, elliptic polarization microscope, resistance detection, capacitance detecting, inductance detections, waveguide detection, white light interference spectrum, surface etc. first is sharp from spectral technique etc. for the method that detects by mark.Detection can adopt a kind of detection method also can adopt two kinds or two or more detection methods simultaneously.
The material of the detection by assembled type multifunctional sensor device comprises tiny organism bodies such as cell, bacterium, virus, biology, chemical micromolecule, gases such as albumen, nucleic acid etc. are biological, the big molecule of chemistry, ion, sugar, amino acid.
Describe the present invention referring now to the following examples, these embodiment only are used to explain the present invention and are not used for limiting scope of the present invention.
Embodiment one:
It is smooth earlier to obtain the about 50 microns upper surface of big or small homogeneous diameter by injection molding, diameter is about the granules of polystyrene that includes magnetic-particle of tumbler structure of 50 microns circular surface, handling its upper surface by the nitrogen plasma gas then makes its surface have amino, connect hepatitis A antibody with connecting the reagent glutaraldehyde then, obtain to detect the sensing function unit of hav antigen.Repeat above-mentioned steps, the prepared in batches surface is the polystyrene sensing function unit that can detect hepatitis B, third liver that includes magnetic-particle of the tumbler structure of square and rhombus respectively.Above-mentioned three kinds of sensing function unit are respectively got one, and to put into a loam cake transparent, it is smooth to go to the bottom, the about one centimetre little box of diameter in, and under magnetic agitation, react with the human serum sample, and then by magnetic force fixedly the sensing function unit be on the sensing transducing platform in going to the bottom, and clean with reagent, clean again with behind fluorescently-labeled hepatitis A antibody, hbv antibody, the c-hepatitis antibody solution reaction, can judge whether contain hepatitis A, hepatitis B, hepatitis C virus among the human serum sample by microscopic examination pattern and fluorescence.
Embodiment two:
It by microballoon synthetic method prepared in batches diameter earlier 10 microns silicon dioxide microsphere, immerse in 1% the amine propyl group three own TMOS ethanolic solutions reaction then after 2 hours, take out the cleaning back and connect anti-Avidin and influenza B virus monoclonal antibody respectively in batches with glutaraldehyde, a kind of antibody in influenza A virus monoclonal antibody and the influenza virus C monoclonal antibody, then respectively with mark different spectral responses the zinc selenide quantum dot particle Avidin and the reaction of above-mentioned three kinds of microballoons and clean after these three kinds of microballoons put into a detachable centrifuge tube cover embedded microcavity, have on the microcavity towards the micropore of centrifuge tube inside.The centrifuge tube of microcavity above-mentioned three kinds of sensing function unit are housed is mark quantum dot microsphere injects the solution of blood sample to be analyzed and fluorescently-labeled three kinds of influenza virus polyclonal antibodies in lid, cover the lid of centrifuge tube and the cell in the centrifugal removal blood, then centrifuge tube is inverted and under proper temperature, is rocked hybrid reaction, just put centrifuge tube then and separating sensing function unit microballoon and serum mixed liquor, making sensing function unit microballoon stand on the substrate that centrifuge tube covers the band micropore in the centrifuge tube of microcavity is on the sensing transducing platform, is undertaken whether containing three kinds of influenza viruses in quantum dot-labeled identification microballoon and the fluorescence labeling identification institute working sample by microscope then.
Embodiment three:
Earlier the diameter that includes the superparamagnetism magnetic-particle by microballoon synthetic method prepared in batches is 10 microns a silicon dioxide microsphere, immerse in 1% the amine propyl group three own TMOS ethanolic solutions reaction then after 2 hours, take out the cleaning back and connect anti-Avidin and influenza B virus monoclonal antibody respectively in batches with glutaraldehyde, a kind of antibody in influenza A virus monoclonal antibody and the influenza virus C monoclonal antibody, then respectively with mark different spectral responses the zinc selenide quantum dot particle Avidin and the reaction of above-mentioned three kinds of microballoons and clean after these three kinds of microballoons are injected in the micro-fluid chip by an injection orifice.Inject the solution of blood sample to be analyzed and fluorescently-labeled three kinds of influenza virus polyclonal antibodies and under proper temperature, carry out the magnetic agitation hybrid reaction by the another one injection orifice, be positioned at the position that the optical detection system can detect by external cylindricality permanent magnet with the silicon dioxide microsphere that certain frequency will have magnetic-particle then, thereby form periodic signal, noise measuring two dimensional image, institute's images acquired is carried out statistical study carry out whether containing three kinds of influenza viruses in quantum dot-labeled identification microballoon and the fluorescence labeling identification institute working sample.
Embodiment four:
It by microballoon synthetic method prepared in batches diameter earlier 10 microns silicon dioxide microsphere, immerse the reaction after 2 hours in the TMOS ethanolic solution of 1% amine propyl group three then, take out the cleaning back and connect anti-Avidin and influenza B virus monoclonal antibody respectively in batches with glutaraldehyde, a kind of antibody in influenza A virus monoclonal antibody and the influenza virus C monoclonal antibody, then respectively with mark different spectral responses the zinc selenide quantum dot particle Avidin and the reaction of above-mentioned three kinds of microballoons and clean after these three kinds of microballoons are put into 24 orifice plates, and inject the solution of blood sample to be analyzed and fluorescently-labeled three kinds of influenza virus polyclonal antibodies respectively, under proper temperature, rock hybrid reaction then, thereby and in 24 orifice plates, separate with blood sample that comprises analysis and the mixed solution that adds solution by magnetic force fixed magnetic microballoon, clean then, at the bottom of by magnetic force sensing function unit microballoon being positioned at the plate of 24 orifice plates at last, undertaken whether containing three kinds of influenza viruses in quantum dot-labeled identification microballoon and the fluorescence labeling identification institute working sample by microscope at last.
Claims (10)
1, a kind of assembled type multifunctional sensor device based on functional particulate, comprise some identical or different packaged type sensing function unit (1) that the sensing responsive material can detect specific detected material that have, sensing transducing platform (2), frame (4), cover plate (5) and actuation means (3), it is characterized in that the surface is connected with the portable sensing function unit (1) of sensing responsive material can be under the energy field effect of actuation means (3), by sensing transducing platform, cover plate, (6) and detected material effect in the sensing transducing space that frame constitutes, also can under the effect of the energy field of actuation means, carry out separating substances, also can be under the effect of the energy field of actuation means and be positioned to be used on the sensing transducing platform (2) to detect.
2, a kind of assembled type multifunctional sensor device according to claim 1 based on functional particulate, it is characterized in that detected material handling the back or directly introducing sensing transducing space through the external world or auxiliary unit, under the actuation means effect, mix, act on portable sensing function unit (1), other reagent, then under actuation means (3) effect, the sensing function cell location detects under the situation of detected material, other reagent existence or separation on sensing transducing platform (2).
3, a kind of assembled type multifunctional sensor device according to claim 1 based on functional particulate, it is characterized in that described portable sensing function unit is a kind of functional particulate, pattern, material are chosen wantonly, and the tumbler structure that the surface that preferably is connected with the sensing responsive material makes progress, size is between 20 nanometers to 1 centimetre.Functional particulate in same sensor preferably is connected with the surface size unanimity of sensing responsive material or has the functional particulate of comparability surface area.The surface that the sensing function unit is connected with the sensing responsive material can be that the smooth structure that also can be coarse or porous structure but preferably adopt high-specific surface area is to improve sensing capabilities.Can preferably comprise magnetic, electricity or electromagnetic response component in inside, handling sensing function unit for what improve portable sensing function unit.Portable sensing function unit can carry out mark when being used to detect also can not carry out mark, but preferably carries out mark.
4, a kind of assembled type multifunctional sensor device based on functional particulate according to claim 1 is characterized in that the platform of described sensing transducing platform for carrying portable sensing function unit and/or actuation means.Sensing transducing platform can be actual platform and also can be virtual platform.When sensing transducing platform was actual platform, sensing transducing platform can be the sheet material or the sheet material of plane or curved surface, and plane or curved surface can be smooth also can the containing flues of a kang that can carry portable sensing transducing unit.When sensing transducing platform was virtual platform, actuation means can be fixed on portable sensing transducing unit on one or more plane or curved surface that can detect by energy field.
5, a kind of assembled type multifunctional sensor device based on functional particulate according to claim 1 is characterized in that the control mode of described actuation means comprises mixing, location, separation and temperature control.Mixed method has mechanical raking, mechanical mixture, magnetic agitation, ultrasonic mixing, rotating electric field to mix, and localization method has gravitational orientation, location, magnetic field, electric field location, centrifugal force location, centripetal force location, mechanical positioning.Separation method has that filtration, absorption, mechanical fixation separate, centrifugal, extraction, electrophoresis, chromatography.When adopting magnetic force to control, portable sensing function unit preferably comprises magnetic component.Actuation means can be combined with assembled type multifunctional sensor device, also can separate with assembled type multifunctional sensor device.Mechanical actuation means based on the assembled type multifunctional sensor device of functional particulate can have only one, also can have a plurality of.
6, a kind of assembled type multifunctional sensor device according to claim 1 based on functional particulate, it is characterized in that assembled type multifunctional sensor device has cover plate, frame, and cover plate (5), frame are formed transducing space (6) with sensing transducing platform (2).The transducing space can be separated into completely separated or partially separated structural unit by separation structure.Can have injection orifice (501) on the cover plate (5).
7,, it is characterized in that auxiliary unit (7) being installed in the outside or the inside in sensing transducing space (6) according to claim 1,2,5,6 described a kind of assembled type multifunctional sensor devices.Auxiliary unit can realize sampling, purifies, in the separation, enrichment, reagent interpolation, reaction, cleaning, discharging, temperature control, fluid FLOW CONTROL, pressure control function one or more.
8, a kind of preparation method of assembled type multifunctional sensor device is characterized in that may further comprise the steps:
A, at first will not have mould solidification method while prepared in batches by injection molding, microballoon polymerization synthetic method, patterning method, offset printing-etching combined techniques or fluid and obtain the idiosome particle as organic material, inorganic material or the inorganic-organic composite material of preparation material;
Secondly b, the surface is not had the idiosome particle that the connects functional group method by chemistry or physics and make its surface form connection functional group; The surface itself is had the idiosome particle that connects functional group, then directly carry out next step operation;
C, then the method by chemistry or physics above-mentioned have connect functional group the idiosome particle surface fixedly the sensing responsive material promptly make the sensing function unit;
D, comprise flat board by choosing suitable plate again, have the sensing transducing platform of the optional panel in the plane in the hole, array hole that the size of size and corresponding sensing function unit is complementary or curved surface shape sheet material or pattern, also can choose and to be empty sensing transducing platform by the dummy rules face that sensing transducing cell location be controlled in the external energy field as reality;
E, at last with the assembling of the above-mentioned sensing function unit that makes, sensing transducing platform and cover plate, frame, promptly get assembled type multifunctional sensor device of the present invention with actuation means combination assembling or that separate then.
9, the preparation method of assembled type multifunctional sensor device according to claim 8, it is characterized in that described sensing function unit has sensing mark, this sensing mark or when the idiosome preparation of granules, make, or the increase when fixing sensing responsive material, or fixedly increasing behind the sensing responsive material.The method of mark comprises that single or multiple fluorescent material carries out mark, single or multiple color material (comprises dyestuff, non-ferrous metal, the schemochrome material comprises interference, diffraction, photon crystal structure look material) carries out mark, single or multiple luminescent material carries out mark, simple or complex geometric shapes comprises geometric configuration, the size mode is carried out mark, utilize the position to carry out mark, utilize element to comprise that radioactive isotope carries out mark, electrical property carries out mark, magnetic property carries out mark, bar code is carried out mark, and two kinds of above labeling method or two or more compound token methods are carried out mark.
10, assembled type multifunctional sensor device according to claim 1 is characterized in that electromagnetic field detection, the element of assembled type multifunctional sensor device by comprising spectral detection detects, electrical property detects, magnetic property detects, one or more methods in the imaging detection method detect.
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| CN 200610040216 CN1866008A (en) | 2006-05-11 | 2006-05-11 | Assembled type multifunctional sensor device and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103970982A (en) * | 2013-01-31 | 2014-08-06 | 施乐公司 | Control-based Inversion For Estimating A Biological Parameter Vector For A Biophysics Model From Diffused Reflectance Data |
| CN110592989A (en) * | 2019-09-17 | 2019-12-20 | 广东迈诺工业技术有限公司 | Manufacturing method of prefabricated parallel steel wire preformed strand for main cable of suspension bridge |
| CN113167790A (en) * | 2018-10-24 | 2021-07-23 | 凸版印刷株式会社 | Cup for immunoassay, method for manufacturing same, and immunoassay method |
-
2006
- 2006-05-11 CN CN 200610040216 patent/CN1866008A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103970982A (en) * | 2013-01-31 | 2014-08-06 | 施乐公司 | Control-based Inversion For Estimating A Biological Parameter Vector For A Biophysics Model From Diffused Reflectance Data |
| CN113167790A (en) * | 2018-10-24 | 2021-07-23 | 凸版印刷株式会社 | Cup for immunoassay, method for manufacturing same, and immunoassay method |
| CN110592989A (en) * | 2019-09-17 | 2019-12-20 | 广东迈诺工业技术有限公司 | Manufacturing method of prefabricated parallel steel wire preformed strand for main cable of suspension bridge |
| CN110592989B (en) * | 2019-09-17 | 2021-11-30 | 广东迈诺工业技术有限公司 | Manufacturing method of prefabricated parallel steel wire preformed strand for main cable of suspension bridge |
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